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| 1 | Identification of the novel recessive gene pi55(t) conferring resistance to Magnaporthe oryzae显示文摘The elite rice cultivar Yuejingsimiao 2 (YJ2) is characterized by a high level of grain quality and yield, and resistance against Magnaporthe oryzae. YJ2 showed 100% resistance to four fungal populations collected from Guangdong, Sichuan, Liaoning, and Heilongjiang Provinces, which is a higher frequency than that shown by the well-known resistance (R) gene donor cultivars such as Sanhuangzhan 2 and 28zhan. Segregation analysis for resistance with F 2 and F 4 populations indicated the resistance of YJ2 was controlled by multiple genes that are dominant or recessive. The putative R genes of YJ2 were roughly tagged by SSR markers, located on chromosomes 2, 6, 8, and 12, in a bulked-segregant analysis using genome-wide selected SSR markers with F 4 lines that segregated into 3 resistant (R):1 susceptible (S) or 1R:3S. The recessive R gene on chromosome 8 was further mapped to an interval 1.9 cM/152 kb in length by linkage analysis with genomic position-ready markers in the mapping population derived from an F 4 line that segregated into 1R:3S. Given that no major R gene was mapped to this interval, the novel R gene was designated as pi55(t). Out of 26 candidate genes predicted in the region based on the reference genomic sequence of the cultivar Nipponbare, two genes that encode a leucine-rich repeat-containing protein and heavy-metalassociated domain-containing protein, respectively, were suggested as the most likely candidates for pi55(t). | HE XiuYing LIU XinQiong WANG Li WANGLing LIN Fei CHENG YongSheng CHEN ZhaoMing LIAO YaoPing PAN QingHua | 2012 | Science China(Life Sciences)2012，55，2： | 15 |
| 2 | Modeling the radiation balance of different urban underlying surfaces显示文摘An urban net all-wave radiation parameterization scheme is evaluated using annual datasets for 2010 recorded at a Beijing urban observation site.The statistical relationship between observed data and simulation data of net radiation has a correlation coefficient of 0.98 and model efficiency of 0.93.Therefore,it can be used to simulate the radiation balance of Beijing.This study analyzes the variation in the radiation balance for different underlying surfaces.To simulate radiation balance differences,we set four pure land-cover types(forest,grass,roads,and buildings).Keeping all other conditions inputted unchanged,we model the radiation balance by changing the land-cover type.The results show that the effects of different underlying surfaces on radiation differ,and that there is much upward long-wave radiation,accounting for 84.3% of the total radiation energy falling incident on the land surface.The annual averages of net radiation for the four land-cover types are in the range of 38.2-53.4 W/m2.The net radiation of the grass surface is minimal while that of the roads surface is maximal.Additionally,with urbanization the net radiation values of common types of land-cover change,such as conversion from forest to roads,grass to roads,and grass to buildings,all have increasing trends,indicating that net radiation usually increases with urban sprawl. | CUI YaoPing LIU JiYuan HU YunFeng WANG JunBang KUANG WenHui | 2012 | Chinese Science Bulletin2012，57，9： | 9 |
| 3 | 2.5-Dimensional Parylene C micropore array with a large area and a high porosity for high-throughput particle and cell separation显示文摘Large-area micropore arrays with a high porosity are in high demand because of their promising potential in liquid biopsy with a large volume of clinical sample.However,a micropore array with a large area and a high porosity faces a serious mechanical strength challenge.The filtration membrane may undergo large deformation at a high filtration throughput,which will decrease its size separation accuracy.In this work,a keyhole-free Parylene molding process has been developed to prepare a large(>20 mm×20 mm)filtration membrane containing a 2.5-dimensional(2.5D)micropore array with an ultra-high porosity(up to 91.37% with designed pore diameter/space of 100μm/4μm).The notation 2.5D indicates that the large area and the relatively small thickness(approximately 10μm)of the fabricated membranes represent 2D properties,while the large thickness-to-width ratio(10μm/<4μm)of the spaces between the adjacent pores corresponds to a local 3D feature.The large area and high porosity of the micropore array achieved filtration with a throughput up to 180 mL/min(PBS solution)simply driven by gravity.Meanwhile,the high mechanical strength,benefiting from the 2.5D structure of the micropore array,ensured a negligible pore size variation during the high-throughput filtration,thereby enabling high size resolution separation,which was proven by single-layer and multi-layer filtrations for particle separation.Furthermore,as a preliminary demonstration,the prepared 2.5-dimensional Parylene C micropore array was implemented as an efficient filter for rare cancer cell separation from a large volume,approximately 10 cells in 10 mL PBS and undiluted urine,with high recovery rates of 87±13% and 56±13%,respectively. | Yaoping Liu Han Xu Wangzhi Dai Haichao Li Wei Wang | 2018 | Microsystems & Nanoengineering2018，4，1： | 2 |
| 4 | A Compact Implementation of AES S-Box Using Evolutionary Algorithm显示文摘S-Box based on Composite field arithmetic(CFA) technology is optimized by Genetic algorithm(GA)and Cartesian genetic programming(CGP) model for reducing the hardware complexity. After using the CFA technique to map Multiplicative inverse(MI) over GF(2~8)into composite field GF((2~4)~2), the compact MI circuit over GF(2~4) is selected from 100 evolved circuits, and same design method is applied to the compact multiplication circuit over GF(2~2). Compared with the direct implementations, the areas of optimized circuits of MI over GF(2~4) and multiplication over GF((2~2)2) are reduced by66% and 57.69%, respectively. The area reductions for MI over GF(2~8) and the whole of S-Box are up to 59.23%and 56.14%, separately. In 180 nm 1.8V COMS technology,compared to previous works, the S-Box proposed in this paper has the minimum area and minimum power, which are 11.27% and 6.65% smaller than that of the smallest area S-Box, respectively. | LIU Yaoping WU Ning ZHANG Xiaoqiang ZHOU Fang GE Fen | 2017 | Chinese Journal of Electronics2017，26，4： | 2 |
| 5 | The relationships between urban-rural temperature difference and vegetation in eight cities of the Great Plains显示文摘Interpreting the relationship between urban heat island (UHI) and urban vegetation is a basis for understanding the impacts of underlying surfaces on UHL The calculation of UHI intensity (UHII) requires observations from paired stations in both urban and rural areas.Due to the limited number of paired meteorological stations,many studies have used remotely sensed land surface temperature,but these time-series land surface temperature data are often heavily affected by cloud cover and other factors.These factors,together with the algorithm for inversion of land surface temperature,lead to accuracy problems in detecting the UHII,especially in cities with weak UHII.Based on meteorological observations from the Oklahoma Mesonet,a world-class network,we quantified the UHII and trends in eight cities of the Great Plains,USA,where data from at least one pair of urban and rural meteorological stations were available.We examined the changes and variability in urban temperature,UHII,vegetation condition (as measured by enhanced vegetation index,EVI),and evapotranspiration (ET).We found that both UHI and urban cold islands (UCI) occurred among the eight cities during 2000-2014 (as measured by impervious surface area).Unlike what is generally considered,UHII in only three cities significantly decreased as EVI and ET increased (p < 0.1),indicating that the UHI or UCI cannot be completely explained simply from the perspective of the underlying surface.Increased vegetative cover (signaled by EVI) can increase ET,and thereby effectively mitigate the UHI.Each study station clearly showed that the underlying surface or vegetation affects urban-rural temperature,and that these factors should be considered during analysis of the UHI effect over time. | Yaoping CUI Xiangming XIAO Russell B DOUGHTY Yaochen QIN Sujie LIU Nan LI Guosong ZHAO Jinwei DONG | 2019 | Frontiers of Earth Science2019，13，2： | 2 |
| 6 | 35 km amplifier-less four-level pulse amplitude modulation signals enabled by a 23 GHz ultrabroadband directly modulated laser显示文摘The 4-level pulse amplitude modulation(PAM4)based on an 23 GHz ultrabroadband directly modulated laser(DML)was proposed.We have experimentally demonstrated that based on intensity modulation and direct detection(IMDD)56 Gbps per wavelength PAM4 signals transferred over 35 km standard single mode fiber(SSMF)without any optical amplification and we have achieved the bit error rate(BER)of the PAM4 transmission was under 2.9×10–4 by using feed forward equalization(FFE). | Yaoping Xiao Yu Liu Yiming Zhang Haotian Bao Ninghua Zhu | 2020 | Journal of Semiconductors2020，41，3： | 1 |
| 7 | Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway显示文摘Metabolic engineering strategies have been successfully implemented to improve the production of isobutanol,a next-generation biofuel,in Saccharomyces cerevisiae.Here,we explore how two of these strategies,pathway re-localization and redox cofactor-balancing,affect the performance and physiology of isobutanol producing strains.We equipped yeast with isobutanol cassettes which had either a mitochondrial or cytosolic localized isobutanol pathway and used either a redox-imbalanced(NADPH-dependent)or redox-balanced(NADH-dependent)ketol-acid reductoisomerase enzyme.We then conducted transcriptomic,proteomic and metabolomic analyses to elucidate molecular differences between the engineered strains.Pathway localization had a large effect on isobutanol production with the strain expressing the mitochondrial-localized enzymes producing 3.8-fold more isobutanol than strains expressing the cytosolic enzymes.Cofactor-balancing did not improve isobutanol titers and instead the strain with the redox-imbalanced pathway produced 1.5-fold more isobutanol than the balanced version,albeit at low overall pathway flux.Functional genomic analyses suggested that the poor performances of the cytosolic pathway strains were in part due to a shortage in cytosolic Fe-S clusters,which are required cofactors for the dihydroxyacid dehydratase enzyme.We then demonstrated that this cofactor limitation may be partially recovered by disrupting iron homeostasis with a fra2 mutation,thereby increasing cellular iron levels.The resulting isobutanol titer of the fra2 null strain harboring a cytosolic-localized isobutanol pathway outperformed the strain with the mitochondrial-localized pathway by 1.3-fold,demonstrating that both localizations can support flux to isobutanol. | Francesca V.Gambacorta Ellen R.Wagner Tyler B.Jacobson Mary Tremaine Laura K.Muehlbauer Mick A.McGee Justin J.Baerwald Russell L.Wrobel John F.Wolters Mike Place Joshua J.Dietrich Dan Xie Jose Serate Shabda Gajbhiye Lisa Liu Maikayeng Vang-Smith Joshua J.Coon Yaoping Zhang Audrey P.Gasch Daniel Amador-Noguez Chris Todd Hittinger Trey K.Sato Brian F.Pfleger | 2022 | Synthetic and Systems Biotechnology2022，7，2： | 1 |
| 8 | A micropore array-based solid lift-off method for highly efficient and controllable cell alignment and spreading显示文摘Interpretation of cell–cell and cell-microenvironment interactions is critical for both advancing knowledge of basic biology and promoting applications of regenerative medicine.Cell patterning has been widely investigated in previous studies.However,the reported methods cannot simultaneously realize precise control of cell alignment and adhesion/spreading with a high efficiency at a high throughput.Here,a novel solid lift-off method with a micropore array as a shadow mask was proposed.Efficient and precise control of cell alignment and adhesion/spreading are simultaneously achieved via an ingeniously designed shadow mask,which contains large micropores(capture pores)in central areas and small micropores(spreading pores)in surrounding areas contributing to capture/alignment and adhesion/spreading control,respectively.The solid lift-off functions as follows:(1)protein micropattern generates through both the capture and spreading pores,(2)cell capture/alignment control is realized through the capture pores,and(3)cell adhesion/spreading is controlled through previously generated protein micropatterns after lift-off of the shadow mask.High-throughput(2.4–3.2×10^(4) cells/cm 2)cell alignments were achieved with high efficiencies(86.2±3.2%,56.7±9.4%and 51.1±4.0%for single-cell,double-cell,and triple-cell alignments,respectively).Precise control of cell spreading and applications for regulating cell skeletons and cell–cell junctions were investigated and verified using murine skeletal muscle myoblasts.To the best of our knowledge,this is the first report to demonstrate highly efficient and controllable multicell alignment and adhesion/spreading simultaneously via a simple solid lift-off operation.This study successfully fills a gap in literatures and promotes the effective and reproducible application of cell patterning in the fields of both basic mechanism studies and applied medicine. | Tingting Hun Yaoping Liu Yechang Guo Yan Sun Yubo Fan Wei Wang | 2020 | Microsystems & Nanoengineering2020，6，1： | 1 |
| 9 | Nanostructure Forma- tion and Passivation of Large-Area Black Silicon for Solar Cell Applications显示文摘 | Liu Yaoping I_ai Tao Li Hailing | 2012 | Small2012，8，9： | 1 |
| 10 | Continued Increases of Gross Primary Production in Urban Areas during 2000-2016显示文摘Urbanization affects vegetation within city administrative boundary and nearby rural areas.Gross primary production(GPP)of vegetation in global urban areas is one of important metrics for assessing the impacts of urbanization on terrestrial ecosystems.To date,very limited data and information on the spatial-temporal dynamics of GPP in the global urban areas are available.In this study,we reported the spatial distribution and temporal dynamics of annual GPP during 2000–2016 from 8,182 gridcells(0.5°by 0.5°latitude and longitude)that have various proportion of urban areas.Approximately 79.3%of these urban gridcells had increasing trends of annual GPP during 2000-2016.As urban area proportion(%)within individual urban gridcells increased,the means of annual GPP trends also increased.Our results suggested that for those urban gridcells,the negative effect of urban expansion(often measured by impervious surfaces)on GPP was to large degree compensated by increased vegetation within the gridcells,mostly driven by urban management and local climate and environment.Our findings on the continued increases of annual GPP in most of urban gridcells shed new insight on the importance of urban areas on terrestrial carbon cycle and the potential of urban management and local climate and environment on improving vegetation in urban areas. | Yaoping Cui Xiangming Xiao Jinwei Dong Yao Zhang Yuanwei Qin Russell BDoughty Xiaocui Wu Xiaoyan Liu Joanna Joiner Berrien Moore III | 2022 | Journal of Remote Sensing2022，，1： | 0 |
| 11 | Enhanced YOLOv5 network-based object detection(BALFilter Reader)promotes PERFECT filter-enabled liquid biopsy of lung cancer from bronchoalveolar lavage fluid(BALF)显示文摘Liquid biopsy of cancers,detecting tumor-related information from liquid samples,has attracted wide attentions as an emerging technology.Our previously reported large-area PERFECT(Precise-Efficient-Robust-Flexible-Easy-ControllableThin)filter has demonstrated competitive sensitivity in recovering rare tumor cells from clinical samples.However,it is time-consuming and easily biased to manually inspect rare target cells among numerous background cells distributed in a large area(Φ≥13 mm).This puts forward an urgent demand for rapid and bias-free inspection.Hereby,this paper implemented deep learning-based object detection for the inspection of rare tumor cells from large-field images of PERFECT filters with hematoxylin-eosin(HE)-stained cells recovered from bronchoalveolar lavage fluid(BALF).CenterNet,EfficientDet,and YOLOv5 were trained and validated with 240 and 60 image blocks containing tumor and/or background cells,respectively.YOLOv5 was selected as the basic network given the highest mAP@0.5 of 92.1%,compared to those of CenterNet and EfficientDet at 85.2%and 91.6%,respectively.Then,tricks including CIoU loss,image flip,mosaic,HSV augmentation and TTA were applied to enhance the performance of the YOLOv5 network,improving mAP@0.5 to 96.2%.This enhanced YOLOv5 network-based object detection,named as BALFilter Reader,was tested and cross-validated on 24 clinical cases.The overall diagnosis performance(~2 min)with sensitivity@66.7%±16.7%,specificity@100.0%±0.0%and accuracy@75.0%±12.5%was superior to that from two experienced pathologists(10–30 min)with sensitivity@61.1%,specificity@16.7%and accuracy@50.0%,with the histopathological result as the gold standard.The AUC of the BALFilter Reader is 0.84±0.08.Moreover,a customized Web was developed for a user-friendly interface and the promotion of wide applications.The current results revealed that the developed BALFilter Reader is a rapid,bias-free and easily accessible AI-enabled tool to promote the transplantation of the BALFilter technique.This work can easily expand to other cytopathological diagnoses and improve the application value of micro/nanotechnology-based liquid biopsy in the era of intelligent pathology. | Zheng Liu Jixin Zhang Ningyu Wang Yun’ai Feng Fei Tang Tingyu Li Liping Lv Haichao Li Wei Wang Yaoping Liu | 2023 | Microsystems & Nanoengineering2023，9，5： | 0 |
| 12 | Four-inch high quality crack-free AlN layer grown on a hightemperature annealed AlN template by MOCVD显示文摘In this work,based on physical vapor deposition and high-temperature annealing(HTA),the 4-inch crack-free high-quality AlN template is initialized.Benefiting from the crystal recrystallization during the HTA process,the FWHMs of X-ray rocking curves for(002)and(102)planes are encouragingly decreased to 62 and 282 arcsec,respectively.On such an AlN template,an ultra-thin AlN with a thickness of~700 nm grown by MOCVD shows good quality,thus avoiding the epitaxial lateral over-growth(ELOG)process in which 3-4μm AlN is essential to obtain the flat surface and high crystalline quality.The 4-inch scaled wafer provides an avenue to match UVC-LED with the fabrication process of traditional GaN-based blue LED,therefore significantly improving yields and decreasing cost. | Shangfeng Liu Ye Yuan Shanshan Sheng Tao Wang Jin Zhang Lijie Huang Xiaohu Zhang Junjie Kang Wei Luo Yongde Li Houjin Wang Weiyun Wang Chuan Xiao Yaoping Liu Qi Wang Xinqiang Wang | 2021 | Journal of Semiconductors2021，42，12： | 0 |
| 13 | Some possible station layouts and characteristics of evacuated tube transportation显示文摘Since Maglev vehicles will run in a closed vacuum tube,the layout of the terminal stations of evacuated tube transportation(ETT) will differ from the traditional railway stations.This paper deals with some possible station layouts of ETT,e.g.,a station with an airlock,a station without an airlock,above ground and underground stations,and stations with either level arrayed or rotation platforms.Then different station layouts are compared,and characteristics of each are analyzed.Finally,a more secure mode for ETT station layouts is suggested,which can be the basis for future ETT station layout and designs. | Yaoping ZHANG Benlin LIU Yong ZHAO | 2011 | Journal of Modern Transportation2011，19，3： | 0 |
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