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| 1 | A micropore array-based solid lift-off method for highly efficient and controllable cell alignment and spreading显示文摘Interpretation of cell–cell and cell-microenvironment interactions is critical for both advancing knowledge of basic biology and promoting applications of regenerative medicine.Cell patterning has been widely investigated in previous studies.However,the reported methods cannot simultaneously realize precise control of cell alignment and adhesion/spreading with a high efficiency at a high throughput.Here,a novel solid lift-off method with a micropore array as a shadow mask was proposed.Efficient and precise control of cell alignment and adhesion/spreading are simultaneously achieved via an ingeniously designed shadow mask,which contains large micropores(capture pores)in central areas and small micropores(spreading pores)in surrounding areas contributing to capture/alignment and adhesion/spreading control,respectively.The solid lift-off functions as follows:(1)protein micropattern generates through both the capture and spreading pores,(2)cell capture/alignment control is realized through the capture pores,and(3)cell adhesion/spreading is controlled through previously generated protein micropatterns after lift-off of the shadow mask.High-throughput(2.4–3.2×10^(4) cells/cm 2)cell alignments were achieved with high efficiencies(86.2±3.2%,56.7±9.4%and 51.1±4.0%for single-cell,double-cell,and triple-cell alignments,respectively).Precise control of cell spreading and applications for regulating cell skeletons and cell–cell junctions were investigated and verified using murine skeletal muscle myoblasts.To the best of our knowledge,this is the first report to demonstrate highly efficient and controllable multicell alignment and adhesion/spreading simultaneously via a simple solid lift-off operation.This study successfully fills a gap in literatures and promotes the effective and reproducible application of cell patterning in the fields of both basic mechanism studies and applied medicine. | Tingting Hun Yaoping Liu Yechang Guo Yan Sun Yubo Fan Wei Wang | 2020 | Microsystems & Nanoengineering2020，6，1： | 1 |
| 2 | 支气管肺泡灌洗液对肺结节鉴别诊断的临床意义显示文摘基于BALF的液态活检能够从细胞和分子层面提供病变信息,可以克服影像学检查难以确诊、组织活检取样技术复杂且困难等问题,有助于肺结节性质的早期判定,对优化肺结节的诊断流程具有重要意义。本文探讨BALF的肺癌液态活检技术对肺结节性质鉴别的价值,分析不同方法的优势、局限性和应用过程的注意事项,思考相关技术未来的发展方向并提出建议,以期促进前沿技术与现有临床诊断模式的有机结合,有望为肺癌的早期诊断提供有力帮助。 | 刘姚萍 冯云爱 邓润 李晋鹏 聂立功 李海潮 王玮 | 2023 | 中华结核和呼吸杂志2023，46，7： | 0 |
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