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| 1 | Expression of co-stimulator 4-1BB molecule in hepatocellular carcinoma and adjacent non-tumor liver tissue,and its possible role in tumor immunity显示文摘AIM:To investigate the expression of 4-1BB molecule in hepatocellular carcinoma (HCC) and its adjacent tissues.METHODS:Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the gene expression of 4-1BB in hepatocarcinoma and its adjacent tissues,and peripheral blood mononuclear cells (PBMCs) from both HCC and health control groups.Flow cytometry was used to analyse the phenotypes of T cell subsets from the blood of HCC patients and healthy volunteers,and further to determine whether 4-1BB molecules were also expressed on the surface of CD4+ and CD8+ T cells. The localization of 4-1BB proteins on tumor infiltrating T cells was determined by direct immunofluorescence cytochemical staining and detected by confocal microscopy.RESULTS:4-1BB mRNA, which was not detectable in normal liver,was found in 19 liver tissues adjacent to tumor edge(<1.0cm).Low expression of 4-1BB mRNA was shown in 8 tumor tissues and 6 liver tissues located within 1 to 5cm away from tumor edge. In PBMCs, 4-1BB mRNA was almost not detected. Percentage of CD4^+, CD8^+ and CD3^+/CD25^+ T cells, as well as ratio of CD4 to CD8 revealed no difference between groups (P>0.05,respectively),while a significant lower percentage of CD3^+ T cell was found in HCC group as compared to healthy control group (P<0.05).However, 4-1BB molecules were almost not found on the surface of CD4+ and CD8+ T cells in HCC and healthy control group.Double-staining of 4-1BB^+/CD4^+ and 4-1BB^+/CD8^+ immunofluorescence on tumor infiltrating T cells was detected in 13 liver tissues adjacent to tumor edge (<1.0cm) by confocal microscopy.CONCLUSION: Although HCC may escape from immune attack by weak immunogenicity or downregulated expression of MHC-1 molecules on the tumor cell surface,tumor infiltrating T cells can be activated via other costimulatory signal pathways to exert a limited antitumor effect on local microenvironment. The present study also implicates that modulating 4-1BB/4-1BBL costimulatory pathway may be an effective immunotherapy strategy to augment the host response. | Wan, YL Zheng, SS Zhao, ZC Li, MW Jia, CK Zhang, H | 2004 | World Journal of Gastroenterology2004，10，2： | 6 |
| 2 | Effect of IFNα-2a on Fas expression and apoptosis rate of peripheral blood cytotoxic T cells in patients with hepatitis B显示文摘Interferon(IFN) with antiviral and im-munomodulatory activities is one of the most important therapeutic agents for the treatment of chronic hepatitis. The apoptotic effect of IFN is influenced by cell type and the types of IFN, which suppresses proliferation and induces apoptosis in some cell types while inhibiting apoptosis in others. The aim of this study was to explore the effect of IFNα-2a on Fas expression and the apoptosis rate of peripheral blood cytotoxic T cells (CTLs) in patients with hepatitis B. METHODS:Peripheral blood mononuclear cells were isolated from 26 patients with hepatitis B including 16 patients with chronic hepatitis B and 10 patients with chronic severe hepatitis B. Fas expression and apoptosis rate of CTLs were analyzed with flow cytometry before and after IFNα-2a treatment. RESULTS:Before IFNα-2a treatment, Fas expression and apoptosis rate of CTLs from patients with chronic hepatitis B were significantly higher than those from patients with chronic severe hepatitis B and healthy controls respectively. No significant difference was observed between Fas expression and apoptosis rate of CTLs from patients with chronic severe hepatitis B and healthy controls. After IFNα-2a treatment,Fas expression and apoptosis rate of CTLs from different groups were compared with those before IFNα-2a treatment, showing no significant difference despite alternation of different degree. CONCLUSIONS:Activation induced cell death (AICD) exists in peripheral blood CTLs from patients with hepatitis B. No effect of IFNα-2a exerts on Fas expression and apoptosis rate of Fas in patients with hepatitis B. | Institute of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou 310003, China (Hou W, Liu KZ, Li MW and Wo JE) | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 4 |
| 3 | Accelerated maturation of primate testis by xenografting into mice显示文摘 | Honaramooz A Li MW Penedo MC | 2004 | Biol Reprod2004，70，： | 2 |
| 4 | Preservation of endo- thelium- dependent relaxation in atherosclerotic mice with endo- thelium- restricted endothelin-1 overexpression显示文摘 | Mian MO Idris-Khodja N Li MW | 2013 | J Pharmacol Exp Ther2013，347，1： | 1 |
| 5 | Combined 1H MR spectroscopy and diffusion-weighted MRI improves the prediction of stroke outcome显示文摘 | Parsons MW Li T Barber PA | 2000 | Neurology2000，55，： | 1 |
| 6 | Genetic complementation in apicomplexan parasites显示文摘 | Striepen B White MW Li C | 2002 | Proc Natl Acad Sci USA2002，99，9： | 1 |
| 7 | Rare ehromones from a fungal mutant of the marine-derived Penicillium purpurogenum G59 显示文摘 | Xia MW Cui CB Li CW et ol | 2015 | MarDrugs2015，13，8： | 1 |
| 8 | Droplet bar- coding for single-cell transcriptomics applied to embryonic stem cells显示文摘 | Klein AM Mazutis L Akartuna I Tallapragada N Veres A Li V Peshkin L Weitz DA Kirschner MW | 2015 | Ceil2015，161，： | 1 |
| 9 | Sparganosis in mainland China 显示文摘 | Li MW Song HQ Li C | 2011 | Int J Infect Dis2011，15，3： | 1 |
| 10 | Minocycline inhibits caspase-1 and caspase-3 expression and delays mortality in a transgenic mouse model of Huntington disease显示文摘 | CHEN M ONA VO LI MW | 2000 | Nat Med2000，6，7： | 1 |
| 11 | Neuroglobin protects PC12 ceils against oxidative stress显示文摘 | Li RC Morris MW Lee SK | 2008 | Brain Res2008，1190，23： | 1 |
| 12 | The pathological characteristics of glioma stem cell niches显示文摘 | He H Li MW Niu CS | 2012 | J Clin Neurosci2012，19，1： | 1 |
| 13 | SirTl inhibition reduces IGF-1/IRS-2/Ras/ERK1/2 signaling and protects neurons显示文摘 | Li Y Xu W McBumey MW | 2008 | Cell Metab2008，8，1： | 1 |
| 14 | Combined l H MR spectrosco- py and diffusion-weighted MRI improves the prediction of stroke out- come显示文摘 | Parsons MW Li T Barber PA | 2000 | Neurology2000，55，： | 1 |
| 15 | Surgical management of late capsular block syndrome 显示文摘 | Qu JF Bao YZ Li MW | 2010 | J Cataract Refract Surg2010，，： | 1 |
| 16 | Neurotoxicity induces cleavage of p35 to p25 by calpain显示文摘 | Lee MS Kwon YT Li MW | 2000 | Nature2000，405，18： | 1 |
| 17 | Expression of Itch in Sertoli cells is controlled via the interaction of E2F1/DP1 complex with E2F and GATA motifs显示文摘 | Li MW Lee WM Lui WY | 2011 | Spermatogenesis2011，1，2： | 1 |
| 18 | Studies on chacacteristics of AmpC- type beta-lactamases of Enterobacter cloacae显示文摘 | Zhang YL Li JT Zhao MW | 2000 | Chin J Antibiot2000，20，6： | 1 |
| 19 | Reappearance and global of variants of influenza B/Victoria/2/87 Lineage viruses in the 2000 - 2001 and 2001 - 2002 seasons 显示文摘 | Shaw MW Xu X Li Y | 2002 | Virology2002，303，1： | 1 |
| 20 | Late postoperative capsular block syndrome versus liquefied after-cataract 显示文摘 | Bao YZ Pei XT Li MW | 2008 | J Cataract Refract Surg2008，34，10： | 1 |
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