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| 1 | Effect of IL-18 on peripheral blood mononuclear cells of chronic hepatitis B and hepatitis B virus DNA released by HepG2.2.15 cell lines显示文摘BACKGROUND: Interleukin-18 (IL-18), a pro-inflamma- tory cytokine that induces interferon-γ (IFN-γ) production in T cells and natural killer cells, plays a critical role in the T-lymphocyte helper type 1 ( Th1) response. This study was designed to explore the effect of IL-18 on peripheral blood mononuclear cells ( PBMCs) derived from chronic hepatitis B (CHB) and on hepatitis B virus (HBV) DNA released by HepG2.2.15 cell lines, which were transfected with hepatitis B virus gene in vitro. METHODS: PBMCs isolated from 25 healthy people and 25 patients with CHB were stimulated with HBcAg and IL-18 of various concentrations for 72 hours. The levels of IFN-γ in the supernatants of cultured PBMCs were determined by ELISA. After the stimulation of IL-18 of various concentra- tions, PBMCs derived from one patient were co-cultured for 96 hours with HepG2. 2. 15 cells which had been cul- tured for 24 hours, and then the supernatants were collected by centrifugation and used for HBV DNA quantitative as- say. RESULTS: When PBMCs were stimulated by HBcAg and IL-18 at various concentrations, the levels of IFN-γ in the supernatants of CHB groups were much higher than those in normal control groups, at 0.2 ng/ml: t =11.70, P< 0.01; at 1.0 ng/ml: t =16.19, P<0.01; and at5.0 ng/ml: t =20.12, P <0.01. In the CHB groups, the levels of IFN-γ in the supernatants of PBMCs stimulated by HBcAg alone were lower than both those stimulated by HBcAg and EL-18 at various concentrations and those stimulated by HBcAg and EL-18 (5.0 ng/ml) together with EL-12 (mild: t = 2.20, P<0.05; moderate; t=2.97, P<0.05; severe; t = 0.66, P >0.05). The content of HBV DNA in the superna- tant of co-cultivation of HepG2. 2. 15 cells and PBMCs without stimulated materials was higher than that stimula-ted by HBcAg and EL-18 at various concentrations of HBc- Ag and IL-18 together with IL-12/IFN-α1lb. CONCLUSION: DL-18 can induce IFN-γ secretion and pro- bably play a key role in the modulation of both innate and adaptive immunity. It has implications in improving im- munoregulatory effect and increasing the ability of immune cells to kill cells infected by virus. | Ying Sun, Huan-Yong Chen and Shao-Jie Xin Harbin, China Department of Infectious Diseases, First Clinical Col- lege, Harbin Medical University, Harbin 150001 , China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，2： | 19 |
| 2 | Factors associated with long-term survival after liver transplantation:A retrospective cohort study显示文摘AIM To identify predictive factors associated with long-term patient and graft survival(> 15 years) in liver transplant recipients.METHODS Medical charts of all de novo adult liver transplant recipients(n = 140) who were transplanted in Hamburg between 1997 and 1999 were retrospectively reviewed.In total,155 transplantations were identified in this time period(15 re-transplantations).Twenty-six orthotopic liver transplant(OLT) recipients were early lost to followup due to moving to other places within 1 year after transplantation.All remaining 114 patients were included in the analysis.The following recipient factors were analysed:Age,sex,underlying liver disease,pre-OLT body mass index(BMI),and levels of alanine aminotransferase(ALT),bilirubin,creatinine and gammaglutamyltransferase(gamma-GT),as well as warm and cold ischemia times.Furthermore,the following donor factors were assessed:Age,BMI,cold ischemia time and warm ischemia time.All surviving patients were followed until December 2014.We divided patients into groups according to their underlying diagnosis:(1) hepatocellularcarcinoma(n = 5,4%);(2) alcohol toxic liver disease(n = 25,22.0%);(3) primary sclerosing cholangitis(n = 6,5%);(4) autoimmune liver diseases(n = 7,6%);(5) hepatitis C virus cirrhosis(n = 15,13%);(6) hepatitis B virus cirrhosis(n = 21,19%);and(7) other(n = 35,31%).The group 'other' included rare diagnoses,such as acute liver failure,unknown liver failure,stenosis and thrombosis of the arteria hepatica,polycystic liver disease,Morbus Osler and Caroli disease.RESULTS The majority of patients were male(n = 70,61%).Age and BMI at the time point of transplantation ranged from 16 years to 69 years(median:53 years) and from 15 kg/m^2 to 33 kg/m^2(median:24),respectively.Sixty-six OLT recipients(58%) experienced a follow-up of 15 years after transplantation.Recipient's age(P = 0.009) and BMI(P = 0.029) were identified as risk factors for death by χ~2-test.Kaplan-Meier analysis confirmed BMI or age above the median as predictors of decreased long-term survival(P = 0.008 and P = 0.020).Hepatitis B as underlying disease showed a trend for improved long-term survival(P = 0.049,χ~2-test,P = 0.055;Kaplan-Meier analysis,Log rank).Pre-transplant bilirubin,creatinine,ALT and gamma-GT levels were not associated with survival in these patients of the pre-era of the model of end stage liver disease.CONCLUSION The recipients' age and BMI were predictors of longterm survival after OLT,as well as hepatitis B as underlying disease.In contrast,donors' age and BMI were not associated with decreased survival.These findings indicate that recipient factors especially have a high impact on long-term outcome after liver transplantation. | Sven Pischke Marie C Lege Moritz von Wulffen Antonio Galante Benjamin Otto Malte H Wehmeyer Uta Herden Lutz Fischer Bjorn Nashan Ansgar W Lohse Martina Sterneck | 2017 | World Journal of Hepatology2017，9，8： | 5 |
| 3 | The change of Morin transition temperature in nanocrystalline α-Fe_2O_3显示文摘THE magnetic property of the ultrafine particles differs from that ot the bulk materials and has a lot of applications. Because of the size effect, magnetically ordered materials exhibit both superparamagnetism and collective magnetic excitation, and the magnetic transition temperature changes in the ultrafine particles with respect to the bulk materials as well. Bulk α-Fe2O3 is weakly ferromagnetic above the Morin transition temperature TM | LI Haibo, GONG Jie, ZHENG Weitao, CHEN Gang and YU Ruihuang1. Department of Materials Science, Jilin University, Changchun 130023, China 2 Department of Physics, Siping Normal ( lege, Siping 136000, China | 1997 | Chinese Science Bulletin1997，42，4： | 2 |
| 4 | Plane stress yield function for aluminum alloy sheets—part 1: theory显示文摘 | F. Barlat J.C. Brem J.W. Yoon K. Chung R.E. Dick D.J. Lege F. Pourboghrat S.-H. Choi E. Chu | 2002 | International Journal of Plasticity2002，，9： | 2 |
| 5 | A six-component yield function for anisotropic metals显示文摘 | Lege D J Brem J C | 1991 | Inernational Journal of Plasticity1991，427，13： | 1 |
| 6 | Comparison of immer- sion ultrasound biometry and partial coherence iterferome- try for intraocular lens calculation according to Haigis 显示文摘 | Haigis W Lege B Miller N | 2001 | Graefes Arch Clip Exp Ophthalmol2001，138，9： | 1 |
| 7 | A six-component yield function for anisotropic materials显示文摘 | BARLAT F LEGE D J BREM J C | | 0，，07： | 1 |
| 8 | Permeability of soil magnetic composites from flakes of nanocrystalline ribbon 显示文摘 | MAZALEYRAT F LEGE V LEBOUGEO R | 2002 | IEEE Trans Magn2002，38，5： | 1 |
| 9 | Comparison of immersion ul- trasound biometry and partial coherence interferometry for intraocu- lar lens calculation according to Haigis显示文摘 | Haigis W Lege B Miller N | 2000 | Graefes Arch Clin Exp Ophthalmo12000，238，9： | 1 |
| 10 | A six-component yield function for anisotropic materials显示文摘 | BARLAT F LEGE D J BREM J C | 1991 | Int J Plasticity1991，7，： | 1 |
| 11 | Yielding description of solution strengthened aluminium alloys显示文摘 | BARLAT F BECKER R C HAYASHIDA Y MAEDA Y YANAGAWA M CHUNG K BREM J C LEGE D J MATSUI K MURTHA S J HATTORI S | 1997 | Int J Plasticity1997，13，： | 1 |
| 12 | Yield function development for aluminium alloy sheets显示文摘 | BARLAT F MAEDA Y CHUNG K YANAGAWA M BREM J C HAYASHIDA Y LEGE D J MATDUI K MURTHA S J HATTORI S BECKER R C MAKOSEY S | 1997 | J Mech Phys Solids1997，45，1112： | 1 |
| 13 | A six-component yield function for anisotropic materials显示文摘 | BARLAT F LEGE D J BREM J C | 1991 | International Journal of Plasticity1991，7，7： | 1 |
| 14 | Laser interference biometry versus ultrasound bi- ometry in certain clinical conditions 显示文摘 | Lege BA Haigis W | 2004 | Graefes Arch Clin Exp Oph- thalmol2004，242，1： | 1 |
| 15 | Plane stress yield function for aluminum alloy sheets (Part I):Theory显示文摘 | BARLAT F BREM J C YOON J W CHUNG K DICK R E LEGE D J POURBOGHRAT F CHOI S H CHU E | 2003 | Int J Plasticity2003，19，： | 1 |
| 16 | Comparison of immersion ultrasound biometry and partial coherence in- terferometry for intraocular lens calculation according to Halgis显示文摘 | HAIGIS W LEGE B MILLER N SCHNEIDER B | 2000 | Graefes Arch Clin Exp Ophthalmol2000，238，9： | 1 |
| 17 | The feasibility hemoglobin combined with 2 point blood sugar test gest&tional diabetes mellitus显示文摘 | XIANG Jin-cao WANG study of glycosylated in the diagnosing of Medical Co):lege 2014 Yan-yan HUANG Xiao-hang et aI | 2014 | Journal of Bengbu 39(7):2014，，： | 1 |
| 18 | Comparison of immersion ultrasound biometry and partial coherence interferometry for intraocular lens calculation according to Haigis显示文摘 | Higis W Lege B Miller N | 2000 | Graefe''s Arch Chin Exp Ophthalmol2000，238，： | 1 |
| 19 | Static and dynamic rotational eye tracking during LASIK treatment of myopic astigmatism with the Zyoptix laser platform and Advanced Control Eye Tracker显示文摘 | Neuhann IM Lege BA Bauer M | | 0，，： | 1 |
| 20 | Comparison of immersion ultrasound biometry and partial coherence interferometry for intraocular lens calculation according to Haigis显示文摘 | Halgis W Lege B Miller N Schneider B | 2000 | Graefes Arch Clin Exp Ophthalmol2000，238，9： | 1 |
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