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| 1 | 间接荧光抗体法快速诊断海兰褐蛋鸡J亚群禽白血病的研究显示文摘某地区海兰褐蛋鸡发生肿瘤性传染病 ,经临床症状和病理学检查 ,在肝、脾、肾、卵巢、输卵管、肺、骨髓、腺胃、肠道等可见胞浆内充满球形嗜酸性颗粒的骨髓瘤细胞 ,呈灶状或弥漫性的分布。该变化是禽骨髓细胞瘤病病理学的特征性变化 ,具有证病意义。经用特异性抗 J亚群白血病病毒囊膜蛋白 gp85的单克隆抗体的免疫组化方法 ,在病料组织切片上检出病毒抗原 ,从而确诊为蛋鸡 J亚群禽白血病。本研究是尝试用间接荧光抗体法来快速诊断该病。采用特异性抗 J亚群禽白血病病毒囊膜蛋白 gp85单克隆抗体对组织触片作间接荧光抗体试验 ,检测 J亚群禽白血病病毒抗原。在骨髓、食管、肌肉、输卵管、肾都出现了范围广而且很明亮的荧光 ;肝、肺、脾的荧光较为明亮 ;心脏的荧光较弱 ,但清晰可见。表明在病料组织中存在特异性病毒抗原。 | 徐镔蕊 董卫星 何召庆 冯小宇 余春明 张丽 任艳丽 L F LEE Mao-xiang LI | 2002 | 中国兽医杂志2002，38，9： | 49 |
| 2 | Evolving concepts in bone infection: redefining “biofilm”,“acute vs. chronic osteomyelitis”, “the immune proteome” and “local antibiotic therapy”显示文摘Osteomyelitis is a devastating disease caused by microbial infection of bone. While the frequency of infection following elective orthopedic surgery is low, rates of reinfection are disturbingly high. Staphylococcus aureus is responsible for the majority of chronic osteomyelitis cases and is often considered to be incurable due to bacterial persistence deep within bone. Unfortunately, there is no consensus on clinical classifications of osteomyelitis and the ensuing treatment algorithm. Given the high patient morbidity,mortality, and economic burden caused by osteomyelitis, it is important to elucidate mechanisms of bone infection to inform novel strategies for prevention and curative treatment. Recent discoveries in this field have identified three distinct reservoirs of bacterial biofilm including: Staphylococcal abscess communities in the local soft tissue and bone marrow, glycocalyx formation on implant hardware and necrotic tissue, and colonization of the osteocyte-lacuno canalicular network(OLCN) of cortical bone. In contrast, S.aureus intracellular persistence in bone cells has not been substantiated in vivo, which challenges this mode of chronic osteomyelitis. There have also been major advances in our understanding of the immune proteome against S. aureus, from clinical studies of serum antibodies and media enriched for newly synthesized antibodies(MENSA), which may provide new opportunities for osteomyelitis diagnosis, prognosis, and vaccine development. Finally, novel therapies such as antimicrobial implant coatings and antibiotic impregnated 3D-printed scaffolds represent promising strategies for preventing and managing this devastating disease. Here, we review these recent advances and highlight translational opportunities towards a cure. | Elysia A. Masters Ryan P. Trombetta Karen L. de Mesy Bentley Brendan F Boyce Ann Lindley Gill Steven R. Gill Kohei Nishitani Masahiro Ishikawa Yugo Morita Hiromu Ito Sheila N. Bello-Irizarry Mark Ninomiya James D. Brodell Jr. Charles C. Lee Stephanie P. Hao Irvin Oh Chao Xie Hani A. Awad John L. Daiss John R. Owen Stephen L. Kates Edward M. Schwarz Gowrishankar Muthukrishnan | 2019 | Bone Research2019，7，3： | 19 |
| 3 | 气相色谱法在中草药及其制剂中痕量有机氯类农药残留分析上的应用进展显示文摘综述了中草药及其制剂中有机氯类农药残留的气相色谱分析技术,包括样品前处理方法,气相色谱分离分析及确证鉴定技术,对近几年来该领域研究进展进行了总结和讨论。 | 徐晓琴 胡广林 王小如 LEE F S C | 2004 | 分析测试学报2004，23，4： | 15 |
| 4 | 柑桔黄龙病的鉴定和柯赫氏定理(英文)显示文摘黄龙(梢)病被认为是世界柑橘生产上的毁灭性病害已超过一个世纪,但该病害的病原学至今还没有清晰地建立起来。根据16S rRNA基因序列分析,一组被称为'Candidatus Liberibacter species'的难培养细菌被认为与黄龙病相关。然而,要确定'Ca.Liberibacter spp.'是黄龙病病原的柯赫氏定理并没有真正完成。令我们担忧的是,近年来有些文献频频指出'Ca.Liberibacter spp.'是黄龙病的病原,其实该细菌与柑橘寄主的直接病理反应还没有完全确定。我们建议,在黄龙病病原学清楚之前,文献报道在这方面需要有准确的阐述。 | Chen J Deng X Civerolo E L Lee R F Jones J B Zhou C Hartung J S Manjunath K L Brlansky R H | 2011 | 植物病理学报2011，41，2： | 10 |
| 5 | 开关电容PWM DC-DC电压调节模块显示文摘介绍了一种开关电容PWM DC-DC电压调节模块,该变换器是开关电容变换器和PWM变换器的结合,具有如下优点:①开关管的零电压开关(Zero-Voltage-Switching,ZVS);②运用自耦变压器实现同步整流管(Synchronous Rectifier,SR)的自驱动,减小了驱动损耗和体二极管导通损耗;③变压器漏感对效率的影响小,可以使用常规的分立式变压器,从而降低成本;④变换器是单相的,结构简单,应用灵活。在理论分析的基础上,搭建了一台四相700kHz 1.2V/130AVRM原理样机验证了理论分析的正确性。 | 曹文静 金科 Ming Xu F C Lee | 2012 | 电工技术学报2012，27，10： | 8 |
| 6 | 层状边坡渐进破裂与失稳过程数值模拟探讨显示文摘运用岩石破裂与失稳过程分析RFPA2D系统 ,以含层理弱层边坡的渐进破裂与失稳分析为例 ,通过对边坡渐进破裂与失稳发生孕育机制及其锚固控制规律的数值模拟 ,数值模拟再现了含层理弱层和锚固边坡渐进破坏与失稳的发生、发展过程。模拟结果表明 。 | 芮勇勤 贺春宁 王惠勇 陈宇亮 Yue Z Q Lee C F 唐春安 | 2002 | 长沙交通学院学报2002，18，3： | 7 |
| 7 | 经动脉和静脉入路行神经介入手术:美国神经介入外科学会(SNIS)标准与指南委员会共识——美国神经介入外科学会发布显示文摘文章旨在对神经介入手术中建立血管通路的技术和工具进行全面的综述。利用已发表的文章,回顾神经介入手术中血管通路的建立方法、部位、工具及技术相关资料,并根据数据质量、证据水平和适当的专家共识综合给出推荐意见。即使建立血管入路的工具和技术仍在不断发展,但目前的文献和经验仍支持神经介入手术中血管通路建立的一些原则。 | Starke RM Snelling B Al-Mufti F Gandhi CD Lee SK Dabus G Fraser JF 刘焕(译) 王丽娜(译) 贺迎坤(译) 朱良付(译) 李天晓(译) 王子亮(译) 白卫星(译) 薛绛宇(译) 冯光(译) 段光明(译) 何艳艳(译) | 2020 | 中华介入放射学电子杂志2020，8，2： | 5 |
| 8 | MRI,^18F—FDG PET/CT及PET/MR成像评估妇科肿瘤显示文摘MRI和^18F-脱氧葡萄糖(FDG)PET/CT在妇科癌症患者的诊疗中扮演着核心和互补角色。由于治疗通常需要联合手术、放疗以及化疗,因此成像对病情分期和评估预后非常重要。该文综述了运用上述2种成像模式在早期评估3种常见妇科肿瘤(宫颈癌、子宫内膜癌和卵巢上皮肿瘤)中的应用。重点提出了影响治疗的影像特征,以及2种成像方式的相对优势和不足。影像评估先期治疗后肿瘤复发以及后续姑息治疗方法的选择。并对MRI和PET中较新的功能和分子技术进行效果评估。最后,该文还介绍PET/MR成像的初期经验,PET/MR是一种新型技术,很有潜力成为个体化妇科肿瘤治疗方案制订的重要方法。 | 王玉 彭新桂 滕皋军 Susanna I. Lee Catalano OA Dehdashti F | 2018 | 中华核医学与分子影像杂志2018，38，4： | 4 |
| 9 | Charge pump power-factorcorrection technologies part I: concept and principle 显示文摘 | QIAN J R LEE F C | 2000 | IEEE Transition on Power Electronics2000，15，1： | 3 |
| 10 | Reproducibility of thrombus volume quantification in multicenter computed tomography pulmonary angiography studies显示文摘AIM To evaluate reproducibility of pulmonary embolism(PE) clot volume quantification using computed tomography pulmonary angiogram(CTPA) in a multicenter setting.METHODS This study was performed using anonymized data in conformance with HIPAA and IRB Regulations(March 2015-November 2016). Anonymized CTPA data was acquired from 23 scanners from 18 imaging centers using each site's standard PE protocol. Two independent analysts measured PE volumes using a semi-automated region-growing algorithm on an FDA-approved image analysis platform. Total thrombus volume(TTV) was calculated per patient as the primary endpoint. Secondary endpoints were individual thrombus volume(ITV), Qanadli score and modified Qanadli score per patient. Inter-and intra-observer reproducibility were assessed using intra-class correlation coefficient(ICC) and BlandAltman analysis. RESULTS Analyst 1 found 72 emboli in the 23 patients with a mean number of emboli of 3.13 per patient with a range of 0-11 emboli per patient. The clot volumes ranged from 0.0041-47.34 cm3(mean +/-SD, 5.93 +/-10.15 cm3). On the second read, analyst 1 found the same number and distribution of emboli with a range of volumes for read 2 from 0.0041 – 45.52 cm3(mean +/-SD, 5.42 +/-9.53 cm3). Analyst 2 found 73 emboli in the 23 patients with a mean number of emboli of 3.17 per patient with a range of 0-11 emboli per patient. The clot volumes ranged from 0.00459-46.29 cm3(mean +/-SD, 5.91 +/-10.06 cm3). Inter-and intraobserver variability measurements indicated excellent reproducibility of the semi-automated method for quantifying PE volume burden. ICC for all endpoints was greater than 0.95 for inter-and intra-observer analysis. Bland-Altman analysis indicated no significant biases.CONCLUSION Semi-automated region growing algorithm for quantifying PE is reproducible using data from multiple scanners and is a suitable method for image analysis in multicenter clinical trials. | Audrey E Kaufman Alison N Pruzan Ching Hsu Sarayu Ramachandran Adam Jacobi Indravadan Patel Lee Schwocho Michele F Mercuri Zahi A Fayad Venkatesh Mani | 2018 | World Journal of Radiology2018，10，10： | 3 |
| 11 | Increased catabolism and decreased unsaturation of ganglioside in patients with inflammatory bowel disease显示文摘AIM: To investigate whether accelerated catabolism of ganglioside and decreased ganglioside content contribute to the etiology of pro-inflammatory intestinal disease. METHODS: Intestinal mucosa from terminal ileum or colon was obtained from patients with ulcerative colitis or inflammatory Crohn's disease(n = 11) undergoing bowel resection and compared to control samples of normal intestine from patients with benign colon polyps(n = 6) and colorectal cancer(n = 12) in this observational case-control study. Gangliosides and phospholipids of intestinal mucosa were characterized by class and ceramide or fatty acid composition using liquid chromatography triple-quad mass spectrometry. Content and composition of ganglioside classes GM1, GM3, GD3, GD1 a, GT1 and GT3 were compared among subject groups. Content and composition of phospholipid classes phosphatidylcholine(PC) and phosphatidylethanolamine were compared among subject groups. Unsaturation index of individual ganglioside and phospholipid classes was computed and compared among subject groups. Ganglioside catabolism enzymes beta-hexosaminidase A(HEXA) and sialidase-3(NEU3) were measured in intestinal mucosa using western blot and compared among subject groups. RESULTS: Relative GM3 ganglioside content was 2-fold higher(P < 0.05) in intestine from patients with inflammatory bowel disease(IBD) compared to control intestine. The quantity of GM3 and ratio of GM3/GD3 was also higher in IBD intestine than control tissue(P < 0.05). Control intestine exhibited 3-fold higher(P < 0.01) relative GD1 a ganglioside content than IBD intestine. GD3 and GD1 a species of ganglioside containing three unsaturated bonds were present in control intestine, but were not detected in IBD intestine. The relative content of PC containing more than two unsaturated bonds was 30% lower in IBD intestine than control intestine(P < 0.05). The relative content of HEXA in IBD intestine was increased 1.7-fold(P < 0.05) and NEU3 was increased 8.3-fold(P < 0.01) compared to normal intestine. Intestinal mucosa in IBD is characterized by increased GM3 content, decreased GD1 a, and a reduction in polyunsaturated fatty acid constituents in GD3, GD1 a and PC.CONCLUSION: This study suggests a new paradigm by proposing that IBD occurs as a consequence of increased metabolism of specific gangliosides. | John J Miklavcic Glen K Shoemaker Vera C Mazurak M Tom Clandinin Tasha DL Hart Kareena L Schnabl Gordon M Lees Bodil MK Larsen Oliver F Bathe Alan BR Thomson M Tom Clandinin | 2015 | World Journal of Gastroenterology2015，21，35： | 3 |
| 12 | 丁醇同分异构体/汽油混合燃料的燃烧和非常规污染物特性的试验研究(英文)显示文摘本文研究了丁醇同分异构体与汽油混合燃料在进气道喷射的火花点火发动机中的燃烧和排放特性。试验在发动机转速为1200 r/min、发动机负荷为3 bar BMEP 和5 bar BMEP 以及不同混合气空燃比条件下(φ=0.83~1.25)进行,使用了70%体积比的纯汽油和30%体积比的丁醇同分异构体混合燃料(N30,S30,I30 和T30)。结果表明,相比于纯汽油,所有的丁醇同分异构体/汽油混合燃料表现出更高的缸内压力,其中N30 有最高的缸内压力峰值和最早的缸内压力峰值相位;T30 表现出较高的brake燃油消耗率(BSFC)和较低的制动热效率(BTE);相比于其他燃料,N30 的UHC 排放较低而I30 的CO排放较高。在非常规排放物特性方面,相比于纯汽油,丁醇同分异构体/汽油混合燃料有较高的乙醛排放;相比于其他混合燃料,N30 的1,3-丁二烯排放较高。使用丁醇同分异构体/汽油混合燃料可以有效地抑制单环芳烃类质(BTEX)的排放。 | 李元绪 宁智 闫峻豪 Timothy H LEE Chia-fon F LEE | 2019 | Journal of Central South University2019，26，8： | 3 |
| 13 | Total dose effects in composite Nitride- Oxide films显示文摘 | Sung- Chul Lee Raparla A Li Y F | 2000 | IEEE Transactions on Nuclear Science2000，47，6： | 2 |
| 14 | How often are angiotentensin Ⅱ and aldosterrone concentrations raised during chronic ACEinhibitortreatment in cardiacfailure显示文摘 | MAC FADYEN R J LEE A F MORTON J J | 1999 | Heart1999，82，1： | 2 |
| 15 | 用萤光抗体试验筛选能表达鸡马立克病病毒pp38基因的重组昆虫杆状病毒显示文摘用抗鸡马立克病病毒(MDV)的38Kd磷蛋白的单克隆抗体作萤光抗体试验,直接从用可转染性野生型杆状病毒AcMNPV株DNA与含MDVpp38基因的重组转基因质粒载体DNA共同转染的Sf 9单层细胞培养中筛选到能表达pp38基因产物的重组杆状病毒感染斑,并从保存于琼脂醣凝胶上的复印斑中收复并进一步克隆了该基因重组病毒.该法易于实施,即使未能熟练掌握根据杆状病毒多角体来区别野生型杆状病毒斑与基因重组病毒斑的技术,也可顺利地筛选到能表达目的基因的重组合病毒。 | 崔治中 Lee L F | 1992 | 江苏农学院学报1992，13，1： | 2 |
| 16 | Analyzing the H19-and T65-epitopes in 38 kd phosphory-lated protein of Marek's disease viruses and comparing chicken immunological reactions to viruses point-mutated in the epitopes显示文摘DNA sequencing analysis in 38 kd phosphorylated protein (pp38) ORF of Marek's disease viruses (MDV) indicated that all tested 10 virulent strains with different pathotypes had 'A' at base #320 and glutamine at aa#107 while reacted with monoclonal antibody (Mab) H19 in indirect fluorescence antibody test (IFA). However, vaccine strain CVI988 had 'G' at base#320 and arginine at aa#107 instead, when it was negative in IFA with Mab H19. Some strains were also reactive with Mab T65 in IFA while there was 'G' at base #326 and glycine at aa#109, but the other strains, which had 'A' at base #326 and glutamic acid at aa#109, did not react with Mab T65. By comparison of CVI988 to its point mutants CVI/rpp38(AG) and CVI/rpp38(AA) with 1 or 2 base(s) changes at bases #320 and /or #326 of pp38 gene for their reactivity with Mab H19 and T65, it was confirmed that the glutamine at aa#107 and glycine at aa#109 were critical to epitopes H19 and T65 respectively. Immuno-reactions to MDV were compared in SPF chickens inoculated with cloned CVI988 and its mutant CVI/rpp38(AG). It was found that antibody responses to MDV in chickens inoculated with CVI/rpp38(AG) were delayed and significantly lower than that in chickens inoculated with the native CVI988. By differential comparison of antibody titers to different antigens, a third epitope specific to CVI988 and dependent on arginine at aa#107 was suggested to be responsible for the big difference in antibody responses induced by native CVI988 and its mutant. | Lee Lucy F | 2004 | Science China(Life Sciences)2004，47，1： | 2 |
| 17 | Landslide risk assessment and management:an overview显示文摘 | F C Dai C F Lee Y Y Ngai | 2002 | Engineering Geology2002，64，： | 2 |
| 18 | Inverse kinematics of serialchain manipulators显示文摘 | Lee H Y Reinholtz C F | 1996 | ASME Journal of Mechanical Design1996，118，3： | 2 |
| 19 | Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000)显示文摘 | Eng L F Ghirnikar R S Lee Y L | 2000 | Neurochem Res2000，25，910： | 2 |
| 20 | 马立克氏病病毒MEQ蛋白对MDV增殖影响的研究显示文摘在马立克氏病病毒 MDV致病机理的研究中 ,弄清致病基因 meq与病毒增殖之间的关系及其分子机制是十分重要的基础。以重组反转录病毒 (RCAS meq)感染鸡胚成纤维细胞 (CEF) ,使源于 MDV强毒参考株 (v MDV) GA株的 meq基因表达于宿主细胞内 ,然后再用 GA株感染这些细胞。通过利用 MDV强毒株特异的抗 pp3 8单克隆抗体所进行的“黑斑”试验以确定 MDV的增殖水平 ,并与未接种 RCAS meq的CEF进行比较。研究结果发现 ,细胞内表达的 meq基因产物可促进 GA株于体外培养细胞中的感染与增殖(病毒斑数增多 )。根据试验的结果 ,作者认为 meq基因在感染细胞内的表达水平是 MDV增殖以及进而能致病。 | 韦平 Lucy F Lee | 2002 | 广西农业生物科学2002，21，1： | 2 |
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