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| 1 | Genetic characterization and potential molecular dissemination mechanism of tet(31) gene in Aeromonas caviae from an oxytetracycline wastewater treatment system显示文摘Recently, the rarely reported tet(31) tetracycline resistance determinant was commonly found in Aeromonas salmonicida, Gallibacterium anatis, and Oblitimonas alkaliphila isolated from farming animals and related environment. However, its distribution in other bacteria and potential molecular dissemination mechanism in environment are still unknown. The purpose of this study was to investigate the potential mechanism underlying dissemination of tet(31) by analysing the tet(31)-carrying fragments in A. caviae strains isolated from an aerobic biofilm reactor treating oxytetracycline bearing wastewater. Twenty-three A. caviae strains were screened for the tet(31) gene by polymerase chain reaction(PCR). Three strains(two harbouring tet(31), one not) were subjected to whole genome sequencing using the PacBio RSII platform. Seventeen A. caviae strains carried the tet(31) gene and exhibited high resistance levels to oxytetracycline with minimum inhibitory concentrations(MICs)ranging from 256 to 512 mg/L. tet(31) was comprised of the transposon Tn6432 on the chromosome of A. caviae, and Tn6432 was also found in 15 additional tet(31)-positive A. caviae isolates by PCR. More important, Tn6432 was located on an integrative conjugative element(ICE)-like element, which could mediate the dissemination of the tet(31)-carrying transposon Tn6432 between bacteria. Comparative analysis demonstrated that Tn6432 homologs with the structure ISCR2-ΔphzF-tetR(31)-tet(31)-ΔglmM-sul2 were also carried by A. salmonicida, G. anatis, and O. alkaliphila, suggesting that this transposon can be transferred between species and even genera. This work provides the first report on the identification of the tet(31) gene in A. caviae, and will be helpful in exploring the dissemination mechanisms of tet(31) in water environment. | Yanhong Shi Zhe Tian Sébastien Olivier Leclercq Hong Zhang Min Yang Yu Zhang | 2019 | Journal of Environmental Sciences2019，31，2： | 4 |
| 2 | Effects of multisite biventricular pacing in patients with heart failure and intraventricular conduction delay显示文摘 | Cazeau S Leclercq C Lavergne T | | 0，，： | 3 |
| 3 | Frequent mutations of the CA simple sequence repeat in intron 1 of EGFR in mismatch repair-deficient colorectal cancers显示文摘AIM:To investigate the polymorphic simple sequencerepeat in intron 1 of the epidermal growth factor receptorgene(EGFR)(CA-SSRⅠ),which is known to affect theefficiency of gene transcription as a putative target of themismatch repair(MMR)machinery in colorectal tumors.METHODS:The CA-SSRⅠgenotype was analyzed ina total of 86 primary colorectal tumors,selected upontheir microsatellite instability(MSI)status[42 with highfrequency MSI(MSI-H)and 44 microsatellite stable(MSS)]and their respective normal tissue.The effect of the CA-SSRⅠgenotype on the expression of the EGFR gene wasevaluated in 18 specimens using quantitative real-timereverse transcription PCR and immunohistochemistry.RESULTS:Mutations in CA-SSRⅠwere detected in 86%(36 of 42)of MSI-H colorectal tumors and 0%(0 of 44)ofMSS tumors,indicating the EGFR gene as a novel putativespecific target of the defective MMR system(P<0.001).Impaired expression of EGFR was detected in most ofthe colorectal tumors analyzed[6/12(50%)at the mRNAlevel and 15/18(83%)at the peptide level].However,noassociation was apparent between EGFR expression andCA-SSRⅠstatus in tumors or normal tissues.CONCLUSION:Our results suggest that CA-SSRⅠsequence does not contribute to the regulation of EGFRtranscription in colon,and should thus not be consideredas a promising predictive marker for response to EGFRinhibitors in patients with colorectal cancer. | Marie-Pierre Buisine Agnès Wacrenier Christophe Mariette Emmanuelle Leteurtre Fabienne Escande Sana Aissi Amandine Ketele Annette Leclercq Nicole Porchet Thécla Lesuffleur | 2008 | World Journal of Gastroenterology2008，14，7： | 3 |
| 4 | Human B cell growth and differentiation in the spleen of immunodeficient mice显示文摘 | Verhoye L Leclercq G | 2001 | J Immunol2001，166，5： | 1 |
| 5 | A pilot experience with permanent biventricular pacing to treat advanced heart failure显示文摘 | LECLERCQ C CAXEAU S RITTER P | 2000 | Am Heart J2000，140，12： | 1 |
| 6 | Effects of multisite biventricular pacing in patients with heart failure and intraventricular conduction delay显示文摘 | Cazeau S Leclercq C Lavergne T | 2001 | N Engl J Med2001，344，12： | 1 |
| 7 | Development of a carbon-carbon hip prosthesis显示文摘 | Christel P Menuier A S Leclercq B | 1987 | Biomed Mater Res1987，21，： | 1 |
| 8 | Acute hemodynamic effects of biventricular DDD pacing in patients with end- stage heart failure显示文摘 | Cazeau S Le Breton H | 1998 | J Am Coll Cardiol1998，32，： | 1 |
| 9 | Hypoxia-inducible factor 1 a inhibits the fibroblast-like markers type I and type III collagen during hypoxia-induced chondrocyte redifferentiation:hypoxia not only induces type II collagen and aggreean, but it also inhibits type I and type II collagen in the hypoxia-inducible factor 1a-dependent redifferentiation of chondroeytes 显示文摘 | Dural E Leclercq S Elissalde J M | 2009 | Arthritis Rheum2009，60，10： | 1 |
| 10 | Rapid responses to anakinra in patients with refractory adult-onset still's dis- ease显示文摘 | Fitzgerald A A Leclercq S A Yan A | 2005 | Arthritis and rheumatism2005，52，6： | 1 |
| 11 | Comparative effects of permanent biventricular and right-univentricular pacing in heart failure patients with chronic atrial fibrillation显示文摘 | Walker S Linde C | 2002 | Eur Heart J2002，23，22： | 1 |
| 12 | Effects of multisite biventricular pacing in patients with heart failure and intraventricular conduction delay显示文摘 | Cazeau S Leclercq C Lavergne T | | 0，，： | 1 |
| 13 | Development of a carbon-carbon hip prosthesis 显示文摘 | Christel P Meunier A Leclercq S | 1987 | J Biomed Mater Res1987，21，2： | 1 |
| 14 | Long-term benefits of biventricular pacing in congestive heart failure:results from the MUltisite STimulation in cardiomyopathy (MUSTIC)study显示文摘 | Leclercq C Rex S | 2002 | J Am Coll Cardiol2002，40，1： | 1 |
| 15 | Ca2 + currents in compensated hypertrophy and heart failure 显示文摘 | Richard S Leclercq F Lemaire S | 1998 | Cardiovasc Res1998，37，2： | 1 |
| 16 | Vancomycin resistance gene VanC is specific to Enterococcus gallinarum 显示文摘 | Leclercq R Malen S Duval J | 1992 | Antimicrob Agents Chemother1992，36，9： | 1 |
| 17 | Effects of muhisite biventricular pacing in patients with heart failure and intraventricular conduction delay 显示文摘 | Cazeau S Leclercq C Lavergne T | 2001 | N Engl J Med2001，344，： | 1 |
| 18 | J Biomed Mater Res显示文摘 | Christel P Meunier A Leclercq S | 1987 | 21(A2 Suppl):1911987，21，2： | 1 |
| 19 | Effects of multisite biventricular pacing in patients with heart failure and intraventricular conduction delay显示文摘 | Cazeau S Leclercq C lavergne T | 2002 | N Engl J Med2002，344，： | 1 |
| 20 | Effects of multisite biventricular pacing in patients with heart failure and intraventricular conduction delay显示文摘 | Cazeau S Leclercq C Lavergne T | 2001 | N Engl J Med2001，344，： | 1 |
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