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| 1 | The p53-induced lincRNA-p21 derails somatic cell reprogramming by sustaining H3K9me3 and CpG methylation at pluripotency gene promoters显示文摘最近的研究在众多的生物过程增加了我们长 noncoding RNA (lncRNAs ) 的理解,但是很少在体的房间 reprogramming 检验了他们的角色。通过表示介绍并且功能的屏蔽,我们鉴别了大 intergenic noncoding RNA p21 (lincRNA-p21 ) 损害 reprogramming。尤其是, lincRNA-p21 被 p53 导致,但是不在 reprogramming 支持 apoptosis 或房间老朽。相反, lincRNA-p21 与 H3K9 methyltransferase SETDB1 和维护 DNA methyltransferase DNMT1 联系,它被 RNA 有约束力的蛋白质 HNRNPK 便于。因而, lincRNA-p21 由在 pluripotency 基因倡导者支撑 H3K9me3 或 CpG methylation 阻止 reprogramming。我们的结果在 reprogramming 提供卓见进 lncRNAs 的角色并且建立在 p53 和 heterochromatin 规定之间的一个新奇连接。 | Xichen Bao Haitao Wu Xihua Zhu Xiangpeng Guo Andrew P Hutchins Zhiwei Luo Hong Song Yongqiang Chen Keyu Lai Menghui Yin Lingxiao Xu Liang Zhou Jiekai Chen Dongye Wang Baoming Qin Jon Frampton Hung-Fat Tse Duanqing Pei Huating Wang Biliang Zhang Miguel A Esteban | 2015 | Cell Research2015，25，1： | 21 |
| 2 | Up-regulation of mitochondrial chaperone TRAP1 in ulcerative colitis associated colorectal cancer显示文摘AIM:To characterize tumor necrosis factor receptorassociated protein 1(TRAP1)expression in the progression of ulcerative colitis(UC)-associated colorectal cancer.METHODS:Chronic UC is an inflammatory bowel disease that predisposes to colorectal cancer.Immunohistochemical analysis was used to evaluate TRAP1expression on tissue microarrays containing colonic tissues from 42 UC progressors(patients with cancer or dysplasia)and 38 non-progressors(dysplasia/cancer free patients).Statistical analyses of the TRAP1immunohistochemistry staining were performed using Graph Pad Prism.Differences in the TRAP1 level between non-progressors and progressors were tested for statistical significance using the Mann-Whitney test.Receiver operating characteristic curve method was used to quantify marker performance in distinguishing diseased cases from controls.RESULTS:TRAP1 was up-regulated in the colon tissues from UC progressors,but not in the colon tissues from UC non-progressors.Moreover,up-regulation of TRAP1 preceded the neoplastic changes:it was present in both the dysplastic and non-dysplastic tissues of UC progressors.When TRAP1 staining in rectal tissue was used as a diagnostic marker,it could distinguish progressors from non-progressors with 59%sensitivity and 80%specificity.Our study further showed that the increase of TRAP1 expression positively correlated with the degree of inflammation in the colorectal cancer tissues,which could be related to the increased oxidation present in the colonic mucosa from UC progressors.We then investigated the cellular proteome changes underlying oxidative stress,and found that oxidative stress could induce up-regulation of TRAP1 along with several other negative modulators of apoptosis.CONCLUSION:These results suggest that oxidative stress in long standing UC could lead to the increase of cytoprotective protein TRAP1,which in turn could promote cancer progression by preventing or protecting the oxidative damaged epithelial cells from undergoing apoptosis.TRAP1 could be a potential diagnostic marker for UC associated colorectal cancer. | Ru Chen Sheng Pan Keith Lai Lisa A Lai David A Crispin Mary P Bronner Teresa A Brentnall | 2014 | World Journal of Gastroenterology2014，20，45： | 9 |
| 3 | 毛细管电泳法分析蜂花粉中黄酮类化合物(英文)显示文摘使用毛细管电泳法(CE)检测了健康食品中常见的六种黄酮类化合物:橙皮甙、海棠甙、异鼠李素、山奈酚、槲皮素、芦丁。研究了一系列的试验参数,如:pH、缓冲溶液浓度、分离电压以及UV检测器波长等,以确定出最佳条件。使用H3BO3-Na2B4O7缓冲液(pH9.2),各分析物可在8min内分出。相对标准偏差(RSD):8次进样的迁移时间为0.77%~0.93%;峰面积为3.8%~8.6%;各检测限(S/N=3)范围为0.34μg/ml~2.9μg/ml,回收率为80.4%~113.9%。方法简单、灵敏,重现性高,线性好,无须固相萃取前处理,用于蜂花粉分析结果准确。 | 卢挺 Edward P C Lai Jorn C C Yu 胡风祖 | 2006 | 食品科学2006，27，12： | 9 |
| 4 | Activation of human fibroblast-like synoviocytes by uric acid crystals in rheumatoid arthritis显示文摘Hyperuricemia-mediated uric acid crystal formation may cause joint inflammation and provoke the destruction of joints through the activation of inflammasome-mediated innate immune responses.However,the immunopathological effects and underlying intracellular regulatory mechanisms of uric acid crystal-mediated activation of fibroblast-like synoviocytes(FLS)in rheumatoid arthritis(RA)have not been elucidated.Therefore,we investigated the in vitro effects of monosodium urate crystals,alone or in combination with the inflammatory cytokines tumor-necrosis factor(TNF)-a or interleukin(IL)-1b,on the activation of human FLS from RA patients and normal control subjects and the underlying intracellular signaling mechanisms of treatment with these crystals.Monosodium urate crystals were able to significantly increase the release of the inflammatory cytokine IL-6,the chemokine CXCL8 and the matrix metalloproteinase(MMP)-1 from both normal and RA-FLS(all P,0.05).Moreover,the additive or synergistic effect on the release of IL-6,CXCL8 and MMP-1 from both normal and RA-FLS was observed following the combined treatment with monosodium urate crystals and TNF-a or IL-1b.Further experiments showed that the release of the measured inflammatory cytokine,chemokine and MMP-1 stimulated by monosodium urate crystals were differentially regulated by the intracellular activation of extracellular signal-regulated kinase and c-Jun N-terminal kinase pathways but not the p38 mitogen-activated protein kinase pathway.Our results therefore provide a new insight into the uric acid crystal-activated immunopathological mechanisms mediated by distinct intracellular signal transduction pathways leading to joint inflammation in RA. | Da P Chen Chun K Wong Lai S Tam Edmund K Li Christopher WK Lam | 2011 | Cellular & Molecular Immunology2011，8，6： | 5 |
| 5 | Human Immunodeficiency Virus Infection-Associated Mortality during Pulmonary Tuberculosis Treatment in Six Provinces of China显示文摘Objective To investigate the risk factors attributable to tuberculosis-related deaths in areas with human immunodeficiency virus(HIV) infection epidemics. Methods A prospective cohort study of newly registered patients in tuberculosis(TB) dispensaries in six representative Chinese provinces was conducted from September 1, 2009 to August 31, 2011. Risk factors for TB-associated death were identified through logistic regression analysis. Results Of 19,103 newly registered pulmonary TB patients, 925(4.8%) were found to be HIV-positive. Miliary TB and acid-fast bacillus smear-negative TB were more common among these patients. Out of a total of 322(1.7%) deaths that occurred during TB treatment, 85(26%) of the patients were co-infected with HIV. Multivariate analysis revealed that HIV infection was the strongest predictor of death [adjusted odds ratio(aO R) 7.86]. Other significant mortality risk factors included presentation with miliary TB(aO R 4.10; 95% confidence interval: 2.14-7.88), ≥35 years of age(aO R 3.04), non-Han ethnicity(aO R 1.67), and farming as an occupation(aO R 1.59). For patients with TB/HIV co-infection, miliary TB was the strongest risk factor for death(aO R 5.48). A low CD4 count(≤ 200 cells/μL)(aO R 3.27) at the time of TB treatment initiation and a lack of antiretroviral therapy(ART) administration(aO R 3.78) were also correlated with an increased risk of death. Conclusion Infection with HIV was independently associated with increased mortality during TB treatment. Offering HIV testing at the time of diagnosis with TB, early TB diagnosis among HIV/acquired immunodeficiency syndrome patients, and the timely provision of ART were identified as the key approaches that could reduce the number of HIV-associated TB deaths. | LAI Yu Ji LIU Er Yong WANG Li Ming Jamie P MORANO WANG Ning Kaveh KHOSHNOOD ZHOU Lin CHENG Shi Ming | 2015 | Biomedical and Environmental Sciences2015，28，6： | 4 |
| 6 | Design, Synthesis and Biological Evaluation of Isothiazole Based 1,2,4-Trizaole Derivatives显示文摘 | Lai Chen Qifan Wu Zhijin Fan Hongpeng Li Jiwei Li Wenhao Hu Xiumei Liu Nataliya P Belskaya Tatiana Glukhareva Bin Zhao | 2018 | Chinese Journal of Chemistry2018，36，8： | 2 |
| 7 | Preparation of stable micropatterns of gold on cell-adhesion-resistant hydrogels assisted by a hetero-bifunctional macromonomer linker显示文摘Surface patterning is very useful in biomaterial studies, yet it is not easy to prepare a micropattern with cell-adhesion contrast that is stable in a wet environment. Recently, a platform technique of transfer photolithography was invented to fabricate stable metal microarrays on the surface of a cell-adhesion resistant and mechanically biomimetic poly(ethylene glycol) hydrogel; the linker is the key chemical in such a transfer strategy. This article reports the design and synthesis of a hetero-bifunctional macromonomer linker with a thiol group at one end and an acryloyl group at the other end. The bifunctional linker was characterized by GPC and 1H NMR, and the average number of thiol groups in the bifunctional linker was detected by Ellman's reagent. The regent stability under wet conditions was also confirmed by the model reactants. The resultant micropatterned surfaces are meaningful for future studies of cell behaviors on mechanically biomimetic matrixes. | SUN JianGuo GRAETER Stefan V TANG Jian HUANG JingHuan LIU Peng LAI YuXiao YU Lin MAJER Günter SPATZ Joachim P DING JianDong | 2014 | Science China Chemistry2014，57，4： | 2 |
| 8 | Corrosion protection of magnesium by electroactive polypyrrole/paint coatings显示文摘 | Truong V T Lai P K Moore B T | 2000 | Synthetic Metals2000，111，： | 2 |
| 9 | Desferrioxamine in warm reperfusion media decreases liver injury aggravated by cold storage显示文摘AIM:To evaluate whether desferrioxamine decreases ischemia and perfusion injury aggravated by cold storage(CS)in a rat liver perfusion model. METHODS:Isolated rat livers were kept in CS in University of Wisconsin Solution for 20 h at 4℃,then exposed to 25 min of warm ischemia(WI)at 37℃ followed by 2 h of warm perfusion(WP)at 37℃with oxygenated(95%oxygen and 5%carbon dioxide) Krebs-Henseleit buffer.Desferrioxamine(DFO),an iron chelator,was added at different stages of storage,ischemia and perfusion:in CS only,in WI only,in WP only, in WI and perfusion,or in all stages.Effluent samples were collected after CS and after WI.Perfusate samples and bile were collected every 30 min(0,0.5,1,1.5 and 2 h)during liver perfusion.Cellular injury was assessed by the determination of lactate dehydrogenase(LDH) and aspartate aminotransferase(AST)in the effluent and perfusate samples.Total iron was analysed in the perfusate samples.After WP,the liver was collected for the determination of liver swelling(wet to dry ratio) and liver morphological examination(hematoxylin and eosin staining). RESULTS:Increased CS time caused increased liver dysfunction during WP.After 2 h of WP,liver injury was indicated by increased release of AST(0.5 h CS:9.4± 2.2 U/g liver vs 20 h CS:45.9±10.8 U/g liver,P<0.05) and LDH(0.5 h CS:59±14 U/g liver vs 20 h CS:297 ±71 U/g liver,P<0.05).There was an associated increase in iron release into the perfusate(0.5 h CS:0.11 ±0.03μmoL/g liver vs 20 h CS:0.58±0.10μmoL/g liver,P<0.05)and reduction in bile flow(0.5 h CS: 194±12μL/g vs 20 h CS:71±8μL/g liver,P<0.05). When DFO was added during WI and WP following 20 h of CS,release of iron into the perfusate was de- creased(DFO absent 0.58±0.10μmoL/g liver vs DFO present 0.31±0.06μmoL/g liver,P<0.05),and liver function substantially improved with decreased release of AST(DFO absent 45.9±10.8 U/g liver vs DFO present 8.1±0.9 U/g liver,P<0.05)and LDH(DFO absent 297±71 U/g liver vs DFO present 56±7 U/g liver,P<0.05),and increased bile flow(DFO absent 71±8μL/g liver vs DFO present 237±36μL/g liver, P<0.05).DFO was also shown to improve liver morphology after WP.Cellular injury(the release of LDH and AST)was significantly reduced with the addition of DFO in CS medium but to a lesser extent compared to the addition of DFO in WP or WI and perfusion.There was no effect on liver swelling or bile flow when DFO was only added to the CS medium. CONCLUSION:DFO added during WI and perfusion decreased liver perfusion injury aggravated by extended CS. | Peter G Arthur Xian-Wa Niu Wen-Hua Huang Bastiaan DeBoer Ching Tat Lai Enrico Rossi John Joseph Gary P Jeffrey | 2013 | World Journal of Gastroenterology2013，19，5： | 2 |
| 10 | High alpha-fetoprotein level correlates with high stage, early recurrence and poor prognosis of hepatocellular carcinoma : significance of hepatitis virus infection ,age ,p53 ,and beta-catenin mutations显示文摘 | PENG S Y CHEN W J LAI P L | 2004 | Int J Cancer2004，112，1： | 1 |
| 11 | A comparison of the effects of problem-based learning and lecturing on the development of student's critical thinking显示文摘 | Tiwari A Lai P So M | 2006 | Medical Education2006，40，6： | 1 |
| 12 | Upregulation of stromal cell-derived factor 1 (SDF-1) expression in microvasculature endothelial cells in retinal ischemia-reperfusion injury显示文摘 | Lai P Li T Yang J | 2008 | Graefe''s Arch Clin Exp Ophthalmol2008，246，12： | 1 |
| 13 | Electrodeposition of Aluminum in Aluminum Chloride/1-methyl-3-ethylimidazolium Chlorlde显示文摘 | Lai P K Skyllas-kazacos M | 1988 | Journal of Electroanalytical Chemistry1988，248，2： | 1 |
| 14 | Chin Tradit Pat Med 显示文摘 | Liu Z Q Lai X P Wu Y H | 2001 | 23(8): 550-5522001，23，8： | 1 |
| 15 | Nitrogen loss in a nitrifying rotating contactor treating ammonium-rich leachate without organic carbon显示文摘 | Siegrist H Reithaar S Lais P | 1998 | Water Sci Technol1998，37，45： | 1 |
| 16 | Electrodeposition of alumin- ium in aluminium chloridel- metheyl-3- ethylimidzaoliumchloride显示文摘 | Lai P K Skyllas-Kazacos M | 1988 | J Electro Chem1988，248，2： | 1 |
| 17 | Application of decolourised hsian-tsao leaf gum to low-fat salad dressing model emulsions: a rheological study显示文摘 | Lai L S Lin P H | 2004 | Journal of the Science of Food and Agriculture2004，84，11： | 1 |
| 18 | Prevalence of irritable bowel syndrome in Italian rural and urban areas显示文摘 | Usai P Manca R Lai MA | 2010 | Eur J Intern Med2010，21，4： | 1 |
| 19 | Synergistic cytotoxicity of artemisinin and sodium butyrate on human cancer cells显示文摘 | Singh N P Lai H C | | 0，，6： | 1 |
| 20 | Preparation and properties of nano-silica modified nega- tive acrylate photoresist 显示文摘 | Lee C K Don T M Lai W C Chert C C Lin D J Cheng L P | 2008 | Thin Solid Films2008，516，23： | 1 |
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