|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Antisense Oligodeoxynucleotide Inhibits Expression of Re-com binantPorcine Follicle-Stim ulating Horm one Receptor显示文摘To assess the role of follicle stimulating hormone receptor(FSHR) gene expression in regulating expression of FSHR protein in the plasma membrane, the effects of a porcine FSHR cDNA antisense oligodeoxynucleotide (ODN) on FSHR mRNA levels and 125 I FSH binding were determined in Chinese hamster ovary cells expression recombinant porcine FSHR (pFSHR CHO cells). An 18 mer phosphorothioate endcapped antisense ODN that corresponded to the region surrounding the translation initiation codon of the porcine FSHR cDNA was synthesized. An 18 mer nonsense sequence of identical nucleotide composition, which had little homology to known DNA sequences, was synthesized for use as a control. pFSHR CHO cells were cultured in 24 well plates (10 5 cells/well) in the absence or presence of 1 20 μmol/L antisense or nonsense ODN for 24 h and then assayed for porcine FSHR mRNA, using quantitative reverse transcription and competitive polymerase chain reaction, and for 125 I FSH binding activity. Treatment with 10 μmol/L antisense ODN caused a paradoxical increase in porcine FSHR mRNA from 0.89±0.06 to 1.64±0.08 ng/mg total RNA ( P <0.05). Transfection with lipofectamine and 0.33 μmol/L antisense ODN caused an increase in porcine mRNA from 0.95±0.08 to 1.53±0.07 ng/mg total RAN. This was probably due to upregulation of mRNA synthesis resulting from inhibition of porcine FSHR protein translation. The nonsense ODN had no effect on porcine FSHR mRNA. Antisense, but not nonsense, ODN (10 μmol/L) inhibited membrane binding of 125 I FSH by 13.6± 0.8 % ( P <0.05) in 24 h. Treatment of cells with antisense ODN (10 μmol/L) for 48 h resulted in a 76±1.5 % ( P <0.05) inhibition of 125 I FSH binding. In contrast, transfection with lipofectamine and 0.33 μmol/L antisense ODN at 0 h caused a 76.1±1.3 % ( P <0.05) reduction in binding within 24 h. Binding had returned to 52.3±2.3 % ( P < 0.05) of normal by 48 h. These results indicate that an antisense ODN corresponding to the region of the translation start site of the porcine FSHR cDNA is an effective specific inhibitor of porcine FSHR synthesis and that inhibition of receptor synthesis causes a decrease in functional membrane bound FSHR. | ZHU Changhong 1, Mark D. Nixon 2, WANG Yifang 1, Andrew R. LaBarbera 3 1 Center of Reproductive Medicine, Tongji Hospital, Tongji Medical University, Wuhan 430030 2 Department of Obstetrics and Gynecology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0526 3 Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0526 | 1999 | Journal of Huazhong University of Science and Technology(Medical Sciences)1999，19，3： | 2 |
| 2 | Comparison of tissue damage, cleansing and cross-contamination potential during wound cleansing via two methods: lavage and negative pressure wound therapy with instillation 显示文摘 | Allen D LaBarbera LA Bondre IL | 2014 | Int Wound J2014，11，2： | 1 |
| 3 | Premature coronary artery disease in a patient with glycogen storage disease 显示文摘 | Labarbera M Milechman G Dullbeeco F | 2010 | J Invasive Cardiol2010，22，8： | 1 |
| 4 | Follicle-stimulating hormone(FSH)receptors and FSH-responsive adenosine 3',5' -cyclic monophosphate production in porcine granulose cells dechne with follicular growth显示文摘 | | 1994 | Endoer Res1994，20，： | 1 |
| 5 | Consumer Behavior in Web-Based Commerce: An Empirical Study显示文摘 | KOUFARIS M LABARBERA | 2002 | International Journal of Electronic Commerce2002，62，： | 1 |
| 6 | The marine alkaloid naamidine A promotes caspase-dependent apoptosis in tumor cells 显示文摘 | LaBarbera DV Modzelewska K Glazar AI | 2009 | Anticancer Drugs2009，20，： | 1 |
| 7 | A Longitudinal Assessment of Consumer Satisfaction / Dissatisfaction; The Dynamic Aspect of the Cognitive Process 显示文摘 | Priscilla A Labarbera David Mazursky | 1983 | Journal of Marketing Research (pre-1986)1983，20，： | 1 |
| 8 | Synthesis of imidazo quinoxalin 9 ones, benzimidazole analogues of pyrroloiminoquinone marine natural products显示文摘 | LABARBERA D V SKIBO E B | 2005 | Bioorg Med Chem2005，13，： | 1 |
| 9 | Analyzing body size as a factor in ecology and evo- lution 显示文摘 | LaBarbera M | 1989 | Annual Review of Ecology and Systematics1989，20，： | 1 |
| 10 | Principles of design of fluid transport systems in zoology显示文摘 | LaBarbera M | 1990 | Science1990，249，： | 1 |
| 11 | Autoilnmune etiology in premature ovarian failure 显示文摘 | LaBarbera AR Miller MM Ober C | 1988 | Am J Reprod Immunol Microbiol1988，16，3： | 1 |
| 12 | Consumer behavior in web- based commerce: An empirical study显示文摘 | Koufaris M Kambil A LaBarbera P A | 2001 | International Journal of Electronic Commerce2001，6，2： | 1 |
| 13 | Consumer Behavior in web-based Commerce: An empirical study显示文摘 | Koufaris M Kambil A Labarbera P A | 2001 | The International Journal of Electronic Commerce2001，，6： | 1 |
| 14 | Synthesis of imidazo[1,5,4- de ]quinoxalin-9-ones, benzimidazole analogues of pyrroloiminoquinone marine natural products显示文摘 | Daniel V. LaBarbera Edward B. Skibo | 2004 | Bioorganic & Medicinal Chemistry2004，，2： | 1 |
| 15 | Effects of constant mechanical tension on the healing of rabbit flexor tendons显示文摘 | Mass DP Tuel RJ Labarbera M | 1993 | Clin Orthop1993，296，： | 1 |
| 16 | A longitudinal assessment of consumer satisfaction / dissatisfaction : The dynamic aspect of the cognitive process 显示文摘 | LaBarbera P A Mazursky D | 1983 | Journal of Marketing Research ( JMR )1983，20，4： | 1 |
| 17 | Analyzing body size as a factor in ecology and evolution显示文摘 | LaBarbera M | 1989 | Annual Review of Ecology and Systematics1989，20，： | 1 |
| 18 | On the fractal dimension of stream networks 显示文摘 | LaBarbera P Rosso R | 1989 | Water Resources Research1989，25，4： | 1 |
| 19 | Fractal relation of mainstream length to catchment area in river networks 显示文摘 | Rosso R Bacchi B LaBarbera P | 1991 | Water Resources Research1991，27，3： | 1 |
| 20 | A Longitudinal Assessment of Consumer Satisfaction/Dissatisfaction: The Dynamic Aspect of the Cognitive Process 显示文摘 | LaBarbera Priscilla A Mazursky David | 1983 | Journal of Marketing Research1983，，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员