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1A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s).QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China 2003Chinese Science Bulletin2003,48,10:121
2Clinical Features of Adult/Adolescent Atopic Dermatitis and Chinese Criteria for Atopic Dermatitis显示文摘Ping Liu Yan Zhao Zhang-Lei Mu Qian-Jin Lu Qian-Jin L U Li Zhang Xu Yao Min Zheng Yi-Wen Tang Xin-Xiano Lu Xiu-Juan xia You-Kun Lin Yu-Zhen Li Cai-Xia Tu Zhi-Rong Yao Jin-Hua Xu Wei Li Wei Lai Hui-Min Yang Hong-Fu Xie Xiu-Ping Han Zhi-Qiang Xie Xiang Nong Zai-Pei Guo Dan-Qi Deng Tong-Xin Shi Jian-Zhong Zhang 2016Chinese Medical Journal2016,,7:64
3Molecular and cellular characterization during chondrogenic differentiation of adipose tissue-derived stromal cells in vitro and cartilage formation in vivo显示文摘Lin Y Luo E Chen X Liu L Qiao J Yan Z Li Z Tang W Zheng X Tian W 2006中国生物学文摘2006,20,5:31
4Extending the CONSORT Statement to moxibustion显示文摘The STandards for Reporting Interventions in Clinical Trials Of Moxibustion (STRICTOM), in the form of a checklist and descriptions of checklist items, were designed to improve reporting of moxibustion trials, and thereby facilitating their interpretation and replication. The STRICTOM checklist included 7 items and 16 sub-items. These set out reporting guidelines for the moxibustion rationale, details of moxibustion, treatment regimen, other components of treatment, treatment provider background, control and comparator interventions, and precaution measures. In addition, there were descriptions of each item and examples of good reporting. It is intended that the STRICTOM can be used in conjunction with the main CONSORT Statement, extensions for nonpharmacologic treatment and pragmatic trials, and thereby raise the quality of reporting of clinical trials of moxibustion. Further comments will be solicited from the experts of the CONSORT Group, the STRICTA Group, acupuncture and moxibustion societies, and clinical trial authors for optimizing the STRICTOM.Chung-wah Cheng Shu-fei Fu Qing-hui Zhou Tai-xiang Wu Hong-cai Shang Xu-dong Tang Zhi-shun Liu Jia Liu Zhi-xiu Lin Lixing Lao Ai-ping Lü Bo-li Zhang Bao-yan Liu Zhao-xiang Bian 2013Journal of Integrative Medicine2013,11,1:27
5Recent developments and future prospects of Vitreoscilla hemoglobin application in metabolic engineering显示文摘Zhang, L Li, YJ Wang, ZN Xia, Y Chen, WS Tang, KX 2007中国生物学文摘2007,21,10:25
6高级别(WHO G3)胰腺神经内分泌肿瘤分类中形态学和生物学的异质性:包括分化好和分化差的肿瘤显示文摘WHO(2010)分类推荐胰腺神经内分泌肿瘤(PanNETs)的分级建立在核分裂象和Ki-67增殖指数的基础上,2级(G2)PanNETs定义为核分裂象2~20个/10HPF或Ki-67增殖指数在3%~20%之间,3级(G3)胰腺神经内分泌癌(NEC)定义为核分裂象〉20个/10HPF或Ki-67增殖指数〉20%。Basturk O Yang Z Tang L H 魏建国 许春伟 邰艳红 2015临床与实验病理学杂志2015,31,4:25
7Intracellular HMGB1 as a novel tumor suppressor of pancreatic cancer显示文摘尽管有最近的进展, oncogenic K 地岬驾驶的胰腺的 ductal 腺癌(PDAC ) 在最致命的人的癌症之中留在现代医学。PDAC 的致病对内在的染色体不稳定性和外来的发炎激活部分可归因。然而,在在胰腺的 tumorigenesis 的这二个事件之间的分子的连接充分还没被建立了。这里,我们证明(HMGB1 ) 细胞内部的高活动性组盒子 1 显著地压制 oncogenic 由禁止染色体的 K-Ras-driven 胰腺的 tumorigenesis 调停不稳定性的支持 inflammatory nucleosome 版本。任何一个单身者的有条件的基因脱离或在胰的 HMGB1 的两等位基因在出生使老鼠变为极其敏感到先锋损害的 oncogenic K-Ras-driven 开始,包括胰腺的 intraepithelial 瘤, intraductal 乳突的 mucinous 瘤,和 mucinous 膀胱的瘤。在胰的 HMGB1 的损失与染色体重新整理和 telomere 畸形描绘的氧化 DNA 损坏和 chromosomal 不稳定性被联系。这些导致煽动性的 nucleosome 版本并且宣传 K-Ras-driven 胰腺的 tumorigenesis。细胞外的 nucleosomes 支持 interleukin 6 (IL-6 ) 由渗入 macrophages/neutrophils 的分泌物并且提高在胰腺的损害表明激活的 oncogenic K 地岬。到 IL-6 或 histone H3 或为先进 glycation 的受体的大美人的抵销的抗体结束产品都限制表明激活的 K 地岬,阻止癌症开发和转移 / 侵略,并且延长在 Pdx1-Cre 的动物幸存; K 地岬 G12D/+;Hmgb1/ 鼠标。由 glycyrrhizin 的 HMGB1 损失的药理学抑制在煽动性的条件下面在老鼠限制 oncogenic K-Ras-driven tumorigenesis。减少在 PDAC 病人的 HMGB1 的原子、全部的细胞的表示与差的全面幸存相关,在 PDAC 与预示、治疗学的关联作为新奇肿瘤 suppressor 支持细胞内部的 HMGB1。Rui Kang Yangchun Xie Qiuhong Zhang Wen Hou Qingping Jiang Shan Zhu Jinbao Liu Dexing Zeng Haichao Wang David L Bartlet Timothy R Billiar Herbert J Zeh III Michael T Lotze Daolin Tang 2017Cell Research2017,27,7:22
8Exosomes from Suxiao Jiuxin pill-treated cardiac mesenchymal stem cells decrease H3K27 demethylase UTX expression in mouse cardiomyocytes in vitro显示文摘Suxiao Jiuxin 药片(SJP ) 是为在中国的急性冠的症候群的处理的繁体中文药,它包含二个主要部件, tetramethylpyrazine (TMP ) 和龙脑(BOR ) 。因此远然而,在心脏的微型环境上位于 SJP 的有益的效果下面的分子的机制是未知的。心脏的间充质的干细胞(C-MSCs ) 通过 microvesicles (exosomes ) 的版本与 cardiomyocytes (厘米) 交流恢复心脏的动态平衡并且得到修理,部分地通过 epigenetic 规章的机制。在这研究,我们检验了 SJP 处理是否改变了 C-MSC-derived exosomes (SJP-Exos ) 引起 epigenetic 在接受者厘米的色彩的改变。从老鼠心孤立的 C-MSC 是有 SJP (SJP-Exos ) 的 pretreated, TMP (TMP-Exos ) 或 BOR (BOR-Exos ) 。然后, HL-1 房间,一根鼠标 cardiomyocyte 线,从控制与 exosomes 被对待 C-MSCs (Ctrl-Exos ) , SJP-Exos, TMP-Exos 或 BOR-Exos。有 SJP-Exos 的处理显著地增加了 histone 的蛋白质层次 3 离氨酸 27 trimethylation (H3K27me3 ) ,为心脏的 transcriptional 抑制的一个关键 epigenetic 染色质标记,在 HL-1 房间。为了推进,探索 SJP-Exo-mediated H3K27me3 upregulation 的机制,我们在对待 exosome 的 HL-1 房间估计了关键 histone methylases (EZH1, EZH2 和 EED ) 和 demethylases (JMJD3 和 UTX ) 的 mRNA 表示层次。有 SJP-Exo 的处理有选择地在接受者 HL-1 房间压制了 UTX 表示。而且, PCNA,房间复制的一个内长的标记,比在 Ctrl-Exo-treated HL-1 房间在 SJP-Exo-treated HL-1 房间是显著地更高的。这些结果证明 SJP-Exos 增加 cardiomyocyte 增长并且证明 SJP 能调制 C-MSC-derived exosomes 引起 epigenetic 染色质在接受者 cardiomyocytes 改变;因而, SJP-Exos 可能被用来支持 cardiomyocyte 增长。Xiao-fen RUAN Yongdun LI Cheng-wei JU Yan SHEN Wei LEI Can CHEN Yang LI Hong YU Yu-tao LIU II-man KIM Xiao-long WANG Neal L WEINTRAUB Yaoliang TANG 2018Acta Pharmacologica Sinica2018,39,4:23
9Zircon U-Pb and Hf isotopes of volcanic rocks from the Batamayineishan Formation in the eastern Junggar Basin显示文摘内部结构的研究被进行从 Batamayineishan 形成调查 U-Pb 年龄,踪迹元素和玄武岩的锆石的 Hf 同位素。暗岩从练习井圣罐头被获得 1 在 Junggar 盆以内在东方 Luliang 上高举。跟踪所有样品是 magmatic 的锆石表演的元素数据,与类似的 REE 模式,包括积极 Ce (Ce=5.06134 ) ,而是否定 Eu (Eu=0.06 ? 0.55 ) 在重稀土元素元素的异例和丰富。在 25 谷物之中,一致年龄被细分进三个组;300.4 整潲祺潧整的年龄?SU YuPing ZHENG JianPing GRIFFIN William L TANG HuaYun O'REILLY Suzanne Y LIN XiangYang 2010Chinese Science Bulletin2010,55,36:21
10Multi-level decollement zones and detachment deformation of Longmenshan thrust belt,Sichuan Basin,southwest China显示文摘As is well known that many decollement layers were developed in the Longmenshan thrust belt,Si-chuan Basin,China. Through field investigation,explanation of seismic profiles and analysis of the balanced sections,we can divide the decollement zones into 3 categories: (1) the deep level decolle-ment zones,including the crust-mantle decollement layer,intracrustal decollement layer,and presinian basal decollement layer. The main structural styles of their deformation are the crust-mantle decoup-ling detachment deformation,the basal ductile shear deformation,etc.; (2) the middle level decollement zones,including the Cambrian-Ordovician decollement layer,the Silurian decollement layer,etc.,the main structural styles of their deformation are the isopachous fold,the angular fold,the saddle struc-ture,and the combination styles of them; and (3) the shallow level decollement zones,including the Xujiahe Formation decollement layer of Upper Triassic and the Jurassic decollement layers,the main structural styles of their deformation are the thrust-nappe tectonic,the pop-up,the triangle zone ,the duplex,etc. Multi-level decollement zones not only made the Longmenshan thrust belt develop many different deformation styles from deep place to shallow place,but also made some local areas have the superimposition of the tectonic deformation apparently. This study indicates that the multi-level de-collement zones have a very important effect on the shaping and evolution of the Longmenshan thrust belt.TANG LiangJie1,2,3,YANG KeMing4,JIN WenZheng1,2,3,L ZhiZhou4 & YU YiXin1,2,3 1 State Key Laboratory of Petroleum Resource and Prospecting,China University of Petroleum,Beijing 102249,China 2 Key Laboratory for Hydrocarbon Accumulation Mechanism,China University of Petroleum,Ministry of Education,Beijing 102249,China 3 Basin and Reservoir Research Center,China University of Petroleum,Beijing 102249,China 4 Southwest Division Company,SINOPEC,Chengdu 610081,China 2008Science China Earth Sciences2008,51,S2:18
11贝伐珠单抗联合mFOLFOX6与单独使用mFOLFOX6作为一线治疗用于RAS突变型不可切除的结直肠癌局限性肝转移的随机对照试验显示文摘目的:评估贝伐珠单抗联合氟尿嘧啶、亚叶酸钙和奥沙利铂(mFOLFOX6)作为RAS突变型不可切除的结直肠癌肝转移一线治疗的疗效。方法:将2013年10月至2017年12月伴有RAS突变型不可切除的结直肠癌局限性肝转移患者随机分为mFOLFOX6+贝伐珠单抗治疗组(A组)和单独mFOLFOX6治疗组(B组)。肝转移灶的可切除性由本地的多学科讨论小组商讨决定。该研究的主要终点是肝转移灶经过治疗后转化为R0切除的比率。次要终点包括肿瘤反应、生存率和药物毒性。该研究采用区组随机法。潘杰斌 TANG W T REN L LIU T S 2020结直肠肛门外科2020,26,5:17
12Aminoglycoside-induced and non-syndromic hearing loss is associated with the G7444A mutation in the mitochondrial COI/tRNA (Ser(UCN)) genes in two Chinese families显示文摘Zhu Y Qian YP Tang XW Wang JD Yang L Liao ZS Li RH Ji JZ Li ZY Chen JF Choo DI Lu JX Guan MX 2006中国生物学文摘2006,20,4:16
13Multilineage differentiation of adipose-derived stromal cells from GFP transgenic mice显示文摘Lin Y Chen X Yan Z Liu L Tang W Zheng X Li Z Qiao J Li S Tian W 2006中国生物学文摘2006,20,5:16
14非小细胞型胃肠道高级别神经内分泌癌作为独立亚型的病理特征显示文摘Shia J Tang L H Weiser M R 王文军(摘译) 张帆(审校) 2008临床与实验病理学杂志2008,24,4:16
15Incipient motion of sediment in presence of submerged flexible vegetation显示文摘The presence of submerged vegetation on river beds can change the water flow structure and alter the state of sediment motion. In this study,the incipient motion of sediment in the presence of submerged flexible vegetation in open channels was investigated in a laboratory experiment.The vegetation was simulated with flexible rubber cylinders arranged in parallel arrays. The effect of the vegetation density, water depth, and sediment grain size on the incipient motion was investigated. The experimental results indicate that the incipient motion velocity of sediment increases as the vegetation density decreases and the water depth and sediment grain size increase. With flexible plants, the incipient motion velocity of sediment is lower than it is without vegetation, and is larger than it is with rigid vegetation. A general incipient motion velocity equation was derived, which can be applied to both flexible and rigid vegetation conditions.Hao Wang Hong-wu Tang Han-qing Zhao Xuan-yu Zhao Sheng-qi Lü 2015Water Science and Engineering2015,8,1:12
16Formation of active oxygen species on single-atom Pt catalyst and promoted catalytic oxidation of toluene显示文摘Catalytic oxidation of toluene over noble metal catalysts is a representative reaction for elimination of volatile organic compounds(VOCs).However,to fully understand the activation of molecular oxygen and the role of active oxygen species generated in this reaction is still a challenging target.Herein,MgO nanosheets and single-atom Pt loaded MgO(Pt SA/MgO)nanosheets were synthesized and used as catalysts in toluene oxidation.The activation process of molecular oxygen and oxidation performance on the two catalysts were contrastively investigated.The Pt SA/MgO exhibited significantly enhanced catalytic activity compared to MgO.The oxygen vacancies can be easily generated on the Pt SA/MgO surface,which facilitate the activation of molecular oxygen and the formation of active oxygen species.Based on the experimental data and theoretical calculations,an active oxygen species promoted oxidation mechanism for toluene was proposed.In the presence of H2O,the molecular oxygen is more favorable to be dissociated to generate•OH on the oxygen vacancies of the Pt SA/MgO surface,which is the dominant active oxygen species.We anticipate that this work may shed light on further investigation of t10.1007/s12274-020-2765-1he oxidation mechanism of toluene and other VOCs over noble metal catalysts.Shunzheng Zhao Yanfeng Wen Xijun Liu Xianyun Pen Fang Lü Fengyu Gao Xizhou Xie Chengcheng Du Honghong Yi Dongjuan Kang Xiaolong Tang 2020Nano Research2020,13,6:12
17Effect of Morinda officinalis capsule on osteoporosis in ovariectomized rats显示文摘AIM:To explore the therapeutic effects of Morinda officinalis capsules(MOP) on osteoporosis in ovariectomized rats.METHOD:Six-month-old female Sprague–Dawley rats were induced for postmenopausal osteoporosis(PMOP) by bilateral ovariectomy and divided into seven groups as follows:sham-operated group,ovariectomized(OVX) control group,OVX treated with xianlinggubao(XLGB)(270 mg·kg-1·d-1),OVX treated with alendronate sodium(ALN)(3 mg·kg-1·d-1),and OVX treated with Morinda officinalis capsule(MOP) of graded doses(90,270 and 810 mg·kg-1·d-1) groups.Oral treatments were administered daily on the 4th week after ovariectomy and lasted for 12 weeks.The bone mineral density was evaluated by dual-energy X-ray absorptiometry.The tartrate-resistant acid phosphatase(TRAP),alkaline phosphatase(AKP),and osteocalcin(OC) levels in the serum and plasma were determined by standard colorimetric and enzyme immunoassays methods.Bone biomechanical properties and morphological parameters were analyzed by three-point bending test and histomorphometry respectively.RESULTS:Morinda officinalis capsules at all doses were able to significantly prevent the OVX-induced loss of bone mass due to diminishing serum AKP and TRAP levels while elevating OC level in the plasma.Morinda officinalis capsules also enhanced the bone strength and prevented the deterioration of trabecular microarchitecture.CONCLUSION:Morinda officinalis capsules possess potent anti-osteoporotic activity in OVX rats which could be an effective treatment for postmenopausal osteoporosis.LI Ye Lü Shan-Shan TANG Gui-Ying HOU Min TANG Qing ZHANG Xiao-Na CHEN Wei-Hai CHEN Gang XUE Qiang ZHANG Cong-Cong ZHANG Ji-Fen CHEN Yi XU Xiao-Yu 2014Chinese Journal of Natural Medicines2014,12,3:10
18Identification of Orientia tsutsugamushi,spotted fever group and typhus group Rickettsia by duplex and nested PCR methods显示文摘Objective:To identify members of genera of rickettsia and O.tsutsugammhi simultaneously.Methods:Rapid and duplex and nested PCR methods have been established by designing primers based on the conserved regions of heat shock protein GroEL gene.345 mouse viscera samples including liver,spleen and kidney,96 Xenopsylla cheopis and 32 chiggers collected from Hongta areas of Yuxi city,Yunnan province were tested by the new PCR methods.Results:The result of the study showed that the new PCR methods could identify most members of genera -Rickettsia and Orientia simultaneously with 100%specificity and its sensitivity could test one copy per microliter.The results of detection prevalence of rickettsioses in mouse,flea and mites DNA samples showed that the total rickettsia infection rate in mouse was 34.78%(120/345).The total infection rates in R.typhi,O.t Karp and R.sibirica of mouse samples were 28.12%(97/345),19.71%(68/345) and O. 29%(1/345) respectively.Co-infection rates in R.typhi and 0.t Karp of mouse samples were 13.33%(46/ 345).O.t Karp type has been the main epidemic strain in these areas.Conclusion:We concluded that this PCR method could be used to detect multi-genera rickettsia simultaneously.Molecular evidences provided in this and previous studies strongly support that Hongta areas of Yuxi city are a natural focus for typhus and scrub typhus with the common occurrence of their confection.M-C Luan D-Z Yu L Tang L-J Zhang 2008Asian Pacific Journal of Tropical Medicine2008,1,4:10
19Tectonic Evolution of the Middle Frontal Area of the Longmen Mountain Thrust Belt,Western Sichuan Basin,China显示文摘由分析平衡的生气的节和 Longmen 山戳带的沉淀历史,中国,我们断定它经历了五个构造阶段:(1 ) 形成阶段(T 3 x ) Longmen 的缩影,山,早 Indosinian 运动,和 Anxian 构造运动创造了 Longmen 山;(2 ) 稳定的构造阶段(更弱的构造运动导致了 Longmen 山戳带的 J 1) 稍微正在被高举并且稍微减退低岬盆;(3 ) 强烈构造阶段(J2.3 ) ,也就是早 Yanshan 运动;(4 ) 连续构造运动(K-E ) ,也就是迟了的 Yanshan 运动和早喜玛拉雅的运动;并且(5 ) Longmen 山(N-Q ) 的形成,也就是晚喜玛拉雅的运动。在那些构造变丑阶段期间, Anxian 运动和喜玛拉雅的运动在 Longmen 山的形成起了重要作用。喜玛拉雅的运动影响了 Longmen 山大多数;阶层强烈地推进了并且严重地被侵蚀。因为一些越过水坝落下的水被推,在 Longmen 山戳带的中间的部分有某 klippes 在以后向东南构造变丑。JIN Wenzheng TANG Liangjie YANG Keming WAN Guimei Lü Zhizhou YU Yixin 2009Acta Geologica Sinica(English Edition)2009,83,1:10
20Community Dynamics of Seed Rain in Mixed Evergreen Broad-leaved and Deciduous Forests in a Subtropical Mountain of Central China显示文摘Seed dispersal is a key process within community dynamics.The spatial and temporal variations of seed dispersal andthe interspecific differences are crucial for understanding species coexistence and community dynamics.This might alsohold for the mixed evergreen broadleaved and deciduous forests in the mountains of subtropical China,but until now littleexisting knowledge is available for this question.In 2001,we chose to monitor the seed rain process of our mixed evergreenbroad-leaved and deciduous forest communities in Mount Dalaoling National Forest Park,Yichang,Hubei Province,China.The preliminary analyses show obvious variations in seed rain density,species compositions and timing of seed rain amongfour communities.The average seed rain densities of the four communities are 2.43±5.15,54.13±182.75,10.05±19.30and 24.91±58.86 inds./m^2,respectively;about one tenth the values in other studies in subtropical forests of China.In eachcommunity,the seed production is dominated by a limited number of species,and the contributions from the others aregenerally minor.Fecundity of evergreen broadleaved tree species is weaker than deciduous species.The seed rain of fourcommunities begins earlier than September,and stops before December,peaking from early September to late October.The beginning date,ending date and peak times of seed rain are extensively varied among the species,indicating differenttypes of dispersal strategies.According to the existing data,the timing of seed rain is not determined by the climateconditions in the same period,while the density of seed rain may be affected by the disturbances of weather variations ata finer temporal resolution.Ze-Hao Shen Yuan-Yuan Tang Nan Lü Jun Zhao Dao-Xing Li Gong-Fang Wang 2007Journal of Integrative Plant Biology2007,49,9:9
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