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| 1 | Peripheral portal vein-oriented non-dilated bile duct puncture for percutaneous transhepatic biliary drainage显示文摘AIM: To evaluate the efficacy of peripheral portal vein(PV)-oriented non-dilated bile duct(BD) puncture for percutaneous transhepatic biliary drainage(PTBD).METHODS: Thirty-five patients with non-dilated BDs underwent PTBD for the management of various biliary disorders, including benign bilioenteric anastomotic stricture(n = 24), BD stricture(n = 5) associated with iatrogenic BD injury, and postoperative biliary leakage(n = 6). Under ultrasonographic guidance, percutaneous transhepatic puncture using a 21-G needle was performed along the running course of the peripheral targeted non-dilated BD(preferably B6 for right-sided approach, and B3 for left-sided approach) or along the accompanying PV when the BD was not well visualized. This technique could provide an appropriate insertion angle of less than 30° between the puncture needle and BD running course. The puncture needle was then advanced slightly beyond the accompanying PV. The needle tip was moved slightly backward while injecting a small amount of contrast agent to obtain the BD image, followed by insertion of a 0.018-inch guide wire(GW). A drainage catheter was then placed usinga two-step GW method. RESULTS: PTBD was successful in 33(94.3%) of the 35 patients with non-dilated intrahepatic BDs. A rightsided approach was performed in 25 cases, while a left-sided approach was performed in 10 cases. In 31 patients, the first PTBD attempt proved successful. Four cases required a second attempt a few days later to place a drainage catheter. PTBD was successful in two cases, but the second attempt also failed in the other two cases, probably due to poor breath-holding ability. Although most patients(n = 26) had been experiencing cholangitis with fever(including septic condition in 8 cases) before PTBD, only 5(14.3%) patients encountered PTBD procedure-related complications, such as transient hemobilia and cholangitis. No major complications such as bilioarterial fistula or portal thrombosis were observed. There was no mortality in our series.CONCLUSION: Peripheral PV-oriented BD puncture for PTBD in patients with non-dilated BDs is a safe and effective procedure for BD stricture and postoperative bile leakage. | Hiroaki Shimizu Atsushi Kato Tsukasa Takayashiki Satoshi Kuboki Masayuki Ohtsuka Hideyuki Yoshitomi Katsunori Furukawa Masaru Miyazaki | 2015 | World Journal of Gastroenterology2015，21，44： | 10 |
| 2 | Clinicopathological features and prognosis of surgical resected cases of biliary cancer with pancreaticobiliary maljunction显示文摘To the Editor:Pancreaticobiliary maljunction(PBM)is a congenital anomaly in which the pancreatic and bile ducts join anatomically outside of the duodenal wall away from the Oddi’s sphincter.This condition causes the reflux of pancreatic juice into the bile duct under high pressure,resulting in various pathologic changes.The features of PBM patients are common bile duct dilatation,long common channel,and high amylase levels in bile juice.Among them,one of the most significant problems is the development of biliary cancer,including extrahepatic bile duct and gallbladder cancers[1].A nationwide survey in Japan reported biliary cancer in 21.6%of adult patients with PBM concomitant with congenital biliary dilatation,32.1%and 62.3%extrahepatic bile duct and gallbladder cancers,respectively[2]. | Tsukasa Takayashiki Hideyuki Yoshitomi Katsunori Furukawa Satoshi Kuboki Masaru Miyazaki Masayuki Ohtsuka | 2020 | Hepatobiliary & Pancreatic Diseases International2020，19，1： | 3 |
| 3 | 胶原支架上体外三维培养成年大鼠心肌细胞显示文摘背景:前期研究中已经在Atelocollagen胶原支架培养出了具有收缩功能的乳鼠心肌细胞。目的:在Atelocollagen胶原支架上三维培养成年大鼠心肌细胞。方法:将成年SD大鼠心肌细胞经消化培养并纯化后,种植到Atelocollagen胶原支架上进行三维培养。应用倒置显微镜动态观察心肌细胞在Atelocollagen胶原支架上的生长状况;对三维培养的心肌细胞块进行冰冻切片,分别进行肌钙蛋白免疫组织化学和免疫荧光显色,对支架上生长的细胞进行鉴定。结果与结论:成年SD大鼠心肌细胞接种12h开始贴附支架生长,24h与支架汇合、紧密黏附,但心肌细胞并不产生自律性搏动,细胞之间夹杂少量崩解的细胞碎片;48h换液后支架网孔中崩解的细胞被换掉,剩下大部分细胞贴附在支架壁边缘生长。苏木精-伊红染色显示培养48h细胞与支架形成复合体。形态学鉴定证明支架网孔中生长的主要是心肌细胞。免疫组织化学和免疫荧光显色均显示在支架内生长的大部分是心肌细胞,并且紧贴支架,生长良好。 | 王慧玲 李琼 郭志坤 孙永琨 Kuboki Y | 2013 | 中国组织工程研究2013，17，16： | 2 |
| 4 | Osteoblast related gene expression of bone marrowcell during the osteoblastic differentiation induced by type Ⅰ collagen 显示文摘 | Mizuno M Kuboki | 2001 | Bioehen (TO kyo)2001，14，1： | 1 |
| 5 | Loop-mediated isothermalamplification for detection of African trypanosomes显示文摘 | Kuboki N Inoue N Sakurai T | 2003 | J Clin Microbiol2003，41，12： | 1 |
| 6 | Avirulent avain influenza virus as a vaccine strain against a potential human pandemic 显示文摘 | Ayato Takada Noritaaka Kuboki katsunori Okazaki | 1999 | J of Virology1999，73，10： | 1 |
| 7 | High glucose level s enhance TGF-β1-thrombospondin-1 path way in cultured human mesangial cells via mechanisms dependent on glucose-induced PKC activation 显示文摘 | Tada H Kuboki K Nomura K | 2001 | J Diabetes Complications2001，15，： | 1 |
| 8 | Fine mapping of quantitative trait loci Hd-l,Hd-2 and Hd-3,controlling heading date of rice,as single Mendelian factors显示文摘 | YAMAMOTO T KUBOKI Y LIN S Y | 1998 | Theor Appl Genet1998，97，： | 1 |
| 9 | Connective tis- sue growth factor expressed in rat alveolar bone regeneration sites after tooth extraction显示文摘 | Kanyama M Kuboki T Akiyama K | 2003 | Arch Oral Biol2003，，： | 1 |
| 10 | A case of severe acute necrotizing pancreatitis after administration of sitagliptin显示文摘 | Sue M Yoshihara A Kuboki K | | 0，，： | 1 |
| 11 | The validity of clini- cal examination for diagnosing anterior disk displacement without reduction显示文摘 | Yatani H Suzuki K Kuboki T | 1998 | Oral Surg Oral Med Oral Pathol Oral RadiolEndod1998，85，6： | 1 |
| 12 | Clinicopathological sig- nificance of somatic RNF43 mutation and aberrant expression of ring finger protein 43 in intraductal papillary mucinous neoplasms of the pancreas显示文摘 | Sakamoto H Kuboki Y Hatori T | 2015 | Mod Pathol2015，28，2： | 1 |
| 13 | Geometry of carriers controlling phenotypie expression in BMP-induced osteogenesis and chondrogenesis显示文摘 | Kuboki Y Jin Q Takita H | 2001 | J Bone Joint Surg Am2001，831，1： | 1 |
| 14 | Periodontal regeneration fol owing application of basic fibroblast growth factor-2 in combination with beta tricalcium phosphate in class III furcation defects in dogs显示文摘 | Saito A Saito E Kuboki Y | | 0，，02： | 1 |
| 15 | Regulation of endothelial constitutive nitric oxide synthase gene expression in endothelial cells and in vivo : a specific vascular action of insulin显示文摘 | Kuboki K Jiang ZY Takahara N | 2000 | Circulation2000，101，： | 1 |
| 16 | Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors显示文摘 | T. Yamamoto Y. Kuboki S. Y. Lin T. Sasaki M. Yano | 1998 | TAG Theoretical and Applied Genetics (-)1998，，1： | 1 |
| 17 | Activation of peroxisome proliferator activated receptor-gamma during hepatic ischemia is agedependent显示文摘 | Shin T Kuboki S Huber N | 2008 | J Surg Res2008，147，2： | 1 |
| 18 | Fine mapping of quantitative trait loci Hd-1,Hd-2 and Hd-3,controlling heading date of rice,as single Mendelian factors显示文摘 | Kuboki Y Lin S Y | 1998 | Theor AppI Genet1998，97，： | 1 |
| 19 | Nonlinearity analysis of resistor string A/D converters显示文摘 | Kuboki S Kato K Miyakawa N | 1982 | IEEE Trans Circ and Syst1982，29，6： | 1 |
| 20 | Chylous ascites after hepatopancreatobiliary surgery显示文摘 | Kuboki S Shimizu H Yoshidome H | | 0，，04： | 1 |
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