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| 1 | Liver-specific gene expression in mesenchymal stem cells is induced by liver cells显示文摘AIM: The origin of putative liver cells from distinct bone marrow stem cells, e.g. hematopoietic stem cells or multipotent adult progenitor cells was found in recent in vitro studies. Cell culture experiments revealed a key role of growth factors for the induction of liver-specific genes in stem cell cultures. We investigated the potential of rat mesenchymal stem cells (MSC) from bone marrow to differentiate into hepatocytic cells in vitro. Furthermore,we assessed the influence of cocultured liver cells on induction of liver-specific gene expression.METHODS: Mesenchymal stem cells were marked with green fluorescent protein (GFP) by retroviral gene transduction. Clonal marked MSC were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with SCF, HGF,EGF, and FGF-4 alone, or in presence of freshly isolated rat liver cells. Cells in cocultures were harvested and GFP+ or GFP- cells were separated using fluorescence activated cell sorting. RT-PCR analysis for the stem cell marker Thy1 and the hepatocytic markers CK-18, albumin, CK-19,and AFP was performed in the different cell populations.RESULTS: Under the specified culture conditions, rat MSC cocultured with liver cells expressed albumin-, CK-18,CK-19, and AFP-RNA over 3 weeks, whereas MSC cultured alone did not show liver specific gene expression.CONCLUSION: The results indicate that (1) rat MSC from bone marrow can differentiate towards hepatocytic lineage in vitro, and (2) that the microenvironment plays a decisive role for the induction of hepatic differentiation of rMSC. | Claudia Lange Philipp Bassler Michael V. Lioznov Helge Bruns Dietrich Kluth Axel R. Zander Henning C. Fiegel | 2005 | World Journal of Gastroenterology2005，11，29： | 31 |
| 2 | Hepatocytic differentiation of mesenchymal stem cells in cocultures with fetal liver cells显示文摘瞄准:为了与胎儿的肝细胞(FLC ) 和可能性在合作文化调查间充质的干细胞(MSC ) 的 hepatocytic 区别膨胀,区分了 hepatocytic 房间。方法:MSC 被制动火箭与绿荧光灯的蛋白质(GFP ) 标记病毒的基因转导变异。同种细胞的显著 MSC 在用与干细胞补充的fibronectin涂的培养皿和媒介刺激条件的肝下面是也有教养的因素( SCF ), hepatocyte 生长因素( HGF ),表皮的生长因素( EGF ),和成纤维细胞生长因素 4 ( FGF-4 )独自一个,或在刚孤立的 FLC 的存在。在合作文化的房间被收获,并且 GFP+ 或 GFP- 房间用荧光被分开激活的房间排序。为肝 specific 标记 cytokeratin-18 (CK-18 ) 的反向的抄写聚合酶链反应(RT-PCR )( 法新社) ,白朊,和 alpha-fetoprotein 在不同房间人口被执行。结果:在指定文化条件下面,与 FLC co 有教养的老鼠 MSC 超过二个星期表示了白朊, CK-18,和 AFP-RNA。在 wk 3, MSC 失去了 hepatocytic 基因表示,可能由于 cocultured FLC 的增生。FLC 也在合作文化和一个很高的生长潜力显示出稳定的肝 specific 基因表情。结论:从骨髓的老鼠 MSC 能面对 FLC 在试管内区分 hepatocytic 房间,在合作文化的 MSC 的存在也为 FLC 的扩大和区别提供有益的环境。 | Claudia Lange Helge Bruns Dietrich Kluth Axel R Zander Henning C Fiegel | 2006 | World Journal of Gastroenterology2006，12，15： | 23 |
| 3 | Integrated Control of Root and Crown Rot in Sugar Beet: Combined Effects of Cultivar, Crop Rotation, and Soil Tillage显示文摘 | Cord Buhre Christian Kluth Klaus Barcky Bemward M?rl?nder and Mark Varrelmann | 2009 | Plant Disease2009，93，2： | 1 |
| 4 | Theprincipleofnormalandabnormalhindgutdevelopment显示文摘 | KLUTH D HILLEN M L AMBRECHT W | 1995 | PediatrSurg1995，30，： | 1 |
| 5 | New materials anddeposition techniques for highly efficient silicon thinfilmsolar cells显示文摘 | Rech B Kluth O Repmann T | | 0，，： | 1 |
| 6 | Microcrystalline n-i-p solar ceils deposited at 1 nm/s by VHF-PECVD 显示文摘 | FEITKNECHT L KLUTH O ZIEGLER Y | 2001 | Solar Energy Mate and Solar Cells2001，66，8： | 1 |
| 7 | The pathogenesis of omphaloeele and gastroschisis:an unsolved problem显示文摘 | Kluth D Lambrecht WG | 1996 | Pediatr Surg Int1996，11，1： | 1 |
| 8 | New materials and deposition techniques for highly efficient silicon thin film solar cells显示文摘 | Rech B Kluth O Repmann T | 2002 | Solar Energy Materials & Solar Cells2002，74，1234： | 1 |
| 9 | Advanced sensor infrastructure for real time reservoir monitor显示文摘 | Kluth E L E Varham M P Clowes J R | 2000 | Paper SPE Paper 651522000，，： | 1 |
| 10 | 显示文摘 | Kluth O Schope G Hupkes J | 2003 | Thin Solid Films2003，442，： | 1 |
| 11 | Current concepts in the embryology of anoreetal malformations显示文摘 | Lambrecht W | 1997 | Semin Pediatr Surg1997，6，4： | 1 |
| 12 | Studies on the use of lucerne silage source for high-milk-producing dairy cows显示文摘 | Bulang M Kluth H Engelhard T | 2006 | J Anita Physiol Anita-Nutr (Berl)2006，90，34： | 1 |
| 13 | Protein requirements in maintenance hemodialysis patients显示文摘 | Kluthe R Luttgen M Capetiance T | 1978 | Am J Clin Nutr1978，31，： | 1 |
| 14 | IL-3 induces down-regulation of CCR3 protein and mRNA in human eosinophils显示文摘 | Dulkys Y Kluthe C Buschermohle T | 2001 | Immunology2001，167，： | 1 |
| 15 | A note on digest- ibility and energy value for sheep of different grape pomace 显示文摘 | Baumgartel T Kluth H Epperlein K | 2007 | Small Ruminant Research2007，，67： | 1 |
| 16 | Association of diabetes mellitus and metformin use with ontological outcomes of patients with non- muscle-invasive bladder cancer显示文摘 | Rieken M Xylinas E Kluth L | 2013 | BJU Int2013，112，8： | 1 |
| 17 | Texture Etched ZnO∶ Al Coated Glass Substrates for Silicon Based Thin Film Solar Cells显示文摘 | Kluth O Rech B Houben L | 1999 | Thin Solid Films1999，351，： | 1 |
| 18 | A note ondigestibility and energy value for sheep of different grape pomace显示文摘 | Baumg'artel T Kluth H Epperlein K | 2007 | Small rumi- nant research2007，67，23： | 1 |
| 19 | Laparoscopic ver- sus open partial nephrectomy for clinical T1 renal masses:no im pact of surgical approach on perioperative complications and long-term postoperative quality of life显示文摘 | BECKER A PRADEL L KLUTH L | 2015 | World J Urol2015，33，3： | 1 |
| 20 | The demise of strategic groups as an Influence on firm performance: Lessons from the UK plastics industry显示文摘 | Nicholas ORegan Carole Kluth John Parnell | 2011 | Strategic Change2011，，20： | 1 |
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