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| 1 | Micro RNA-320 family is downregulated in colorectal adenoma and affects tumor proliferation by targeting CDK6显示文摘AIM: To investigate the microR NA(miR NA) expression during histological progression from colorectal normal mucosa through adenoma to carcinoma within a lesion. METHODS: Using microarray, the sequential changes in miR NA expression profiles were compared in colonic lesions from matched samples; histologically, nonneoplastic mucosa, adenoma, and submucosal invasive carcinoma were microdissected from a tissue sample. Cell proliferation assay was performed to observe the effect of miR NA, and its target genes were predicted using bioinformatics approaches and the expression profile of SW480 transfected with the miR NA mimics. mR NA and protein levels of the target gene in colon cancer cell lines with a mimic control or miR NA mimics were measured using qR TPCR and Western blotting. The expression levels of miR NA and target gene in colorectal tissue samples were also measured.RESULTS: Microarray analysis identified that the miR-320 family, including miR-320 a, miR-320 b, miR-320 c, miR-320 d and miR-320 e, were differentially expressed in adenomaand submucosal invasive carcinoma. The mi R-320 family, which inhibits cell proliferation, is frequently downregulated in colorectal adenoma and submucosal invasive carcinoma tissues. Seven genes including CDK6 were identified to be common in the results of gene expression array and bioinformatics analyses performed to find the target gene of the miR-320 family. We confirmed that mR NA and protein levels of CDK6 were significantly suppressed in colon cancer cell lines with miR-320 family mimics. CDK6 expression was found to increase from non-neoplastic mucosa through adenoma to submucosal invasive carcinoma tissues and showed an inverse correlation with miR-320 family expression.CONCLUSION: MiR-320 family affects colorectal tumor proliferation by targeting CDK6, plays important role in its growth, and is considered to be a biomarker for its early detection. | Toshihiro Tadano Yoichi Kakuta Shin Hamada Yosuke Shimodaira Masatake Kuroha Yoko Kawakami Tomoya Kimura Hisashi Shiga Katsuya Endo Atsushi Masamune Seiichi Takahashi Yoshitaka Kinouchi Tooru Shimosegawa | 2016 | World Journal of Gastrointestinal Oncology2016，8，7： | 9 |
| 2 | Gastrointestinal polyposis with esophageal polyposis is useful for early diagnosis of Cowden's disease显示文摘Cowden's disease, one of the several hamartoma syndromes, is characterized by hyperplastic lesions and hamartomas distributed in the whole body. About thirty percent of patients with Cowden's disease have been reported to be complicated by malignant tumors. Based on the criteria of the International Cowden Consortium, this disease is mainly diagnosed as trichilemmoma of the face and oral mucosal papillomatosis. However, Cowden's disease patients themselves often do not recognize trichilemmoma of the face and oral mucosal papillomatosis. We report a case of Cowden's disease in a 33-year-old female patient who was diagnosed based on the characteristic findings at gastrointestinal endoscopy. Clinically, the patient was aware of having bloody stools. Multiple polyps found endoscopically in the esophagus, stomach, ileum, colon and rectum showed histopathologically hamartomatous changes and epithelial hyperplasia. Physical examination revealed oral papillomatosis and facial trichilemmomas. A germline mutation in exon 8 of the phosphatase and tensin homolog deleted on chromosome ten (PTEN) gene was found in this case. It was a point mutation of C to T at codon 1003 (CGA→TGA, arginine→stop codon). The characteristic findings on gastrointestinal endoscopy led us to a diagnosis of Cowden's disease. It has been reported that gastrointestinal polyposis with esophageal polyposis is found in about 85.7% of Japanese patients with Cowden's disease. The characteristic findings on gastrointestinal endoscopy can be a useful diagnostic clue to Cowden's disease. | Ken Umemura Sho Takagi Yasushi Ishigaki Masahiro Iwabuchi Shigeru Kuroki Yoshitaka Kinouchi Tooru Shimosegawa | 2008 | World Journal of Gastroenterology2008，14，37： | 6 |
| 3 | Short and long-term outcomes of endoscopic balloon dilatation for Crohn's disease strictures显示文摘AIM:To investigate the short and long-term outcomes of endoscopic balloon dilatation(EBD) for Crohn's disease(CD) strictures.METHODS:Between January 1995 and December 2011,47 EBD procedures were performed in 30 patients(8 females and 22 males) with CD.All patients had strictures through which an endoscope could not pass,and symptoms of these strictures included abdominal pain,abdominal fullness,nausea,and/or vomiting.The 47 strictures included 17 anastomotic and 30 de novo strictures.Endoscopy and dilatation were performed under conscious sedation with intravenous diazepam or flunitrazepam.The dilatations were all performed using through-the-scope balloons with diameters from 8 mm to 20 mm on inflation and lengths of 30-80 mm.Each dilatation session consisted of two to four,3-min multistep inflations of the balloon,repeated at intervals of 1 wk until adequate dilatation(up to 15-20 mm in diameter) was achieved.The follow-up data were collected from medical records and analyzed retrospectively.Primary success was defined as passage of the scope through the stricture after EBD.Longterm outcomes were analyzed focusing on interventionfree survival and surgery-free survival demonstrated by the Kaplan-Meier method.(Intervention-free meant cases in which neither endoscopic balloon re-dilatation nor surgery was needed after the first dilatation during the observation period).The log rank test was used to evaluate the difference in long-term outcomes between anastomotic and de novo stricture cases.RESULTS:Primary success was achieved in 44 of the 47 strictures(93.6%).Balloon dilatations failed in 3 cases(6.4%).In 1 case,EBD was a technical failure because the guide-wire could not be passed through the stricture which showed severe adhesion and was a flexural lesion of the intestine.In 2 cases,unexpected perforations occurred immediately after balloon dilatation.Of the 47 treatments,complications occurred in 5(10.6%).All 5 patients had de novo strictures.One suffered bleeding,two high fever and there were colorectal perforations.One of the patients with a colorectal perforation was treated surgically,the other was managed conservatively.These 2 cases correspond to the two aforementioned EBD failures.Long-term outcomes were evaluated for the 44 successfully-treated strictures after a median follow-up of 26 mo(range,2-172 mo).During the observation period,re-strictures after EBDs occurred in 26 cases(60.5%).Fourteen of these 26 re-stricture cases underwent EBD again,but in two EBD failed and surgery was ultimately performed in both cases.Twelve of the 26 re-stricture cases were initially treated surgically when the re-strictures occurred.Finally,30 of the 47 strictures(63.8%) were successfully managed with EBD,allowing surgery to be avoided.Intervention-free survival evaluated by the Kaplan-Meier method was 75% at 12 mo,58% at 24 mo,and 43% at 36 mo.There was no significant difference between the anastomotic strictures(n = 16) and de novo strictures(n = 28) in the intervention-free survival as evaluated by the log-rank test.Surgery-free survival evaluated by the Kaplan-Meier method was 90% at 12 mo,75% at 24 mo,and 53% at 36 mo.The 16 anastomotic strictures were associated with significantly better surgery-free survivals than the 28 de novo strictures(log-rank test:P < 0.05).CONCLUSION:Anastomotic strictures were associated with better long-term outcomes than de novo strictures,indicating that stricture type might be useful for predicting the long-term outcomes of EBD. | Katsuya Endo Seiichi Takahashi Hisashi Shiga Yoichi Kakuta Yoshitaka Kinouchi Tooru Shimosegawa | 2013 | World Journal of Gastroenterology2013，19，1： | 3 |
| 4 | Introduction of antisense CD44S cDNA down regulates expression of overall CD44 isoforms and inhibits tumor growth and metastasis in highly metastatic colon carcinoma cells显示文摘 | HaradaN Mizoi T Kinouchi M | | 0，，： | 2 |
| 5 | Current Status of Pylorus-Preserving Gastrectomy for the Treatment of Gastric Cancer: A Questionnaire Survey and Review of Literatures显示文摘 | Chikashi Shibata Fumito Saijo Masayuki Kakyo Makoto Kinouchi Naoki Tanaka Iwao Sasaki Takashi Aikou | 2012 | World Journal of Surgery2012，，4： | 2 |
| 6 | Attenuation of focal cerebral ischemic injury in transgenic mice overexpressing CuZn superoxide dismutase 显示文摘 | Kinouchi H Epstein C J Mizui T | 1991 | Proc Natl Acad Sci USA1991，88，11： | 1 |
| 7 | A brief history of excitable map-based neurons and neural networks 显示文摘 | GIRARDI-SCHAPPO M TRAGTENBERG M H KINOUCHI O | 2013 | J Neurosci Methods2013，220，2： | 1 |
| 8 | Measurement of the electrical bio-impedance of breast tumors显示文摘 | Morimoto T Kinouchi Y lritani T | 1990 | Eur Surg Res1990，22，： | 1 |
| 9 | Introduction of antisense CD44S cDNA down-regulates expression of overall CD44 isoforms and inhibits tumor growth and metastasis in highly metastatic colon carcinoma cells显示文摘 | Harada N Mizoi T Kinouchi M | 2001 | Int J Cancer2001，91，1： | 1 |
| 10 | Is it possible to compare researchers with different scientific interests 显示文摘 | BATISTA P D CAMPITELI M G KINOUCHI O | 2006 | Scientometrics2006，68，1： | 1 |
| 11 | Differential diag- nosis of hyponatremia following subarachnoid hemorrhage 显示文摘 | Ogawasara K Kinouchi H Nagamine Y | 1998 | No Shinkei Geka1998，26，6： | 1 |
| 12 | Inflammatory bowel disease:IBD显示文摘 | Endo K Shiga H Kinouchi Y | 2009 | Rinsho Byori2009，57，： | 1 |
| 13 | Combined embolization and microsurgery for cerebral arteriovenous malformation显示文摘 | Kinouchi H Mizoi K Takahashi A | 2002 | Neurol Med Chir (Tokyo)2002，42，9： | 1 |
| 14 | HLAB is the best candidate of susceptibility genes in HLA for Japanese ulcerative colitis 显示文摘 | Aizawa H Kinouchi Y Negoro K | 2009 | Tissue Antigens2009，73，3： | 1 |
| 15 | Effects of cilostazol on cerebral vasospasm after aneurysmal subarach- noid hemorrhage: a multicenter prospective, randomized, open- label blinded end point trial 显示文摘 | Senbokuya N Kinouchi H Kanemaru K | 2012 | J Neurosurg2012，46，5： | 1 |
| 16 | Ipsilateral adrenal involvement from renal cell carcinoma: retrospective study of the predictive value of computed tomography显示文摘 | SAWAI Y KINOUCHI T MANO M | 2002 | Urology2002，59，1： | 1 |
| 17 | Results of modified uncut Roux-en-Y reconstruction after distal gastrectomy for gastric cancer 显示文摘 | Shibata C Kakyo M Kinouchi M | 2013 | Hepatogastroenterology2013，60，127： | 1 |
| 18 | Serum lipid metabolism abnormalities and change in lipoprotein contents in patients with advancedstage renal disease显示文摘 | Tsumura M Kinouchi T Ono S | 2001 | Clin Chim Acta 2001 Dec2001，314，12： | 1 |
| 19 | Noninvasive measurement of the electrical bioimpedance of breast tumors 显示文摘 | Ohmine Y Morimoto T Kinouchi Y | 2000 | Anticancer Res2000，20，3： | 1 |
| 20 | Incidence rate of satellite tumors in renal cell carcinoma 显示文摘 | Kinouchi T Mano M Saiki S | 1999 | Cancer1999，86，11： | 1 |
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