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| 1 | Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata显示文摘Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs. | Anna Pick Kiong LING Kinn Poay TAN Sobri HUSSEIN | 2013 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2013，14，7： | 2 |
| 2 | Occult breast cancer presenting with axillary metastases 显示文摘 | Baron PL Moore MP Kinne DW | 1990 | Arch Surg1990，125，2： | 1 |
| 3 | Major hepatic resection using vascular isolation and hypothermic perfusion显示文摘 | Fortner JG Shiu MH Kinne DW | 1974 | Ann Surg1974，180，4： | 1 |
| 4 | Epithelial transport of magnesium in the kidney of fish显示文摘 | Beyenbach K W Freire C A Kinne R K | 1993 | Miner Electrolyte Metab1993，19，45： | 1 |
| 5 | Estrogtu receptor protein inbreast cancer as a predictor of recurrence显示文摘 | Kinne DW | 1981 | Cancer1981，47，10： | 1 |
| 6 | Cellular uptake of dietary flavonoid quercetin 4'-beta-glucoside by sodium-dependent glucose transporter SGLT1 显示文摘 | Walgren RA Lin JT Kinne RK | 2000 | J Pharmacol Exp Ther2000，294，： | 1 |
| 7 | Preoperative fasting for adults to prevent perioperative complications 显示文摘 | Brady M Kinn S Stuart P | 2008 | Coehrane Datab Syst Rev2008，6，4： | 1 |
| 8 | We now report on ''' versus 'Let us now see how'' ': Author roles and interactionwith readers in research articles 显示文摘 | Flottum K Kinn T Dahl T | 2006 | Academic discourse across disciplines2006，，： | 1 |
| 9 | Breastcarcinoma presenting with axillary lymph node metastasis: An analysis of specific histopathologic features 显示文摘 | Haupt HM Rosen PP Kinne DW | 1999 | Am J Surg Pathol1999，9，3： | 1 |
| 10 | Preoperative fasting for preventing perioperative complications in children显示文摘 | Brady M Kinn S Ness V | 2009 | Cochrane Database Syst Rev2009，18，00： | 1 |
| 11 | Design of a low noise propulsion diesel installation显示文摘 | Kinns R Faulder B M | | INEC0，92，： | 1 |
| 12 | Vegetative Target Enhancement in Natural Scenes Using Multiband Polarization Methods显示文摘 | Duggin M J Kinn G J Bohling E | 1997 | SPIE1997，3121，： | 1 |
| 13 | Proliferation of T-cells, macrophages, neutrophilic granulocytes and fibroblast-like cells in the synovial membrane of patients with rheumatioid arthritis 显示文摘 | Schaser K Kinne RW Beil H | 1996 | Verh Dtsch Ges Pathol1996，80，： | 1 |
| 14 | Minimisation of the sound power radiated by a submarine through optimisation of its resonance changer显示文摘 | MERZ S KESSISSOGLOU N KINNS R | 2010 | Journal of Sound and Vibration2010，329，： | 1 |
| 15 | Occult breast presenting with axillary metastases显示文摘 | Baron PL Moore MP Kinne DW | 1990 | Arch Surg1990，125，： | 1 |
| 16 | Effect of platelet activation on coronary collateral blood flow显示文摘 | Kinn JW Bache RJ | 1998 | Circulation1998，98，： | 1 |
| 17 | Neomysis vulgaris thompsoneine autokologische studies显示文摘 | Kinne O | 1955 | Biologisches Zentralblatt1955，74，： | 1 |
| 18 | Phlorizin recognition in a C-terminal fragment of SGLT1 studied by tryptophan scanning and affinity labeling 显示文摘 | Raja M Tyagi NK Kinne RK | 2003 | Biol Chem2003，278，49： | 1 |
| 19 | Nanoscale proteinpores modified with PAMAM dendrimers 显示文摘 | Hugh Martin Helen Kinns Nick Mitchell ef al | 2007 | Jacs2007，129，： | 1 |
| 20 | Hull vibratory forces transmitted via the fluid and the shaft from a submarine propeller显示文摘 | KINNS R THOMPSON I KESSISSOGLOU N | 2007 | Ships & Offshore Structures2007，2，2： | 1 |
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