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| 1 | Mucosal bacterial microflora and mucus layer thickness in adolescents with inflammatory bowel disease显示文摘AIM:To assess the mucosa-associated bacterial microflora and mucus layer in adolescents with inflammatory bowel disease(IBD) .METHODS:Sixty-one adolescents(mean age 15 years,SD ± 4.13) were included in the study.Intestinal biopsies from inflamed and non-inflamed mucosa of IBD patients and from controls with functional abdominal pain were cultured under aerobic and anaerobic conditions.The number of microbes belonging to the same group was calculated per weight of collected tissue.The mucus thickness in frozen samples was measured under a fluorescent microscope.RESULTS:The ratios of different bacterial groups in inflamed and non-inflamed mucosa of IBD patients and controls were specific for particular diseases.Streptococcus spp.were predominant in the inflamed mucosa of Crohn's disease(CD) patients(80% of all bacteria) ,and Lactobacillus spp.were predominant in ulcerative colitis patients(90%) .The differences were statistically significant(P = 0.01-0.001) .Lower number of bifidobacteria was observed in the whole IBD group.A relation was also found between clinical and endoscopic severity and decreased numbers of Lactobacillus and,to a lesser extent,of Streptococcus in biopsies from CD patients.The mucus layer in the inflamed sites was significantly thinner as compared to controls(P = 0.0033) and to non-inflamed areas in IBD patients(P = 0.031) .CONCLUSION:The significantly thinner mucosa of IBD patients showed a predominance of some aerobes specific for particular diseases,their numbers decreased in relation to higher clinical and endoscopic activity of the disease. | Krzysztof Fyderek Magdalena Strus Kinga Kowalska-Duplaga Tomasz Gosiewski Andrzej Wedrychowicz Urszula Jedynak-Wasowicz Malgorzata Sladek Stanislaw Pieczarkowski Pawel Adamski Piotr Kochan Piotr B Heczko | 2009 | World Journal of Gastroenterology2009，15，42： | 14 |
| 2 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 3 | 瞬时受体电位通道TRPV1、TRPA1和TRPM8拮抗剂在小鼠神经源性和神经病理性疼痛模型中的镇痛作用(英文)显示文摘目的:评价瞬时受体电位通道(TRP通道)TRPV1、TRPA1和TRPM8拮抗剂在小鼠神经源性、持续性和神经病理性疼痛模型中的作用。方法:通过辣椒素实验、异硫氰酸烯丙酯(AITC)实验和福尔马林实验,评估TRP通道拮抗剂在小鼠神经源性疼痛模型中的镇痛作用;通过建立紫杉醇诱导的小鼠神经病理性疼痛模型,对TRP通道拮抗剂的抗痛觉(冷、热、触觉)过敏效应进行评估;通过旋转法实验对小鼠的运动协调性进行评估。结论:TRP通道家族包含了不同的小鼠疼痛模型。TRP通道拮抗剂能减轻神经源性、持续性和神经病理性疼痛,但是其镇痛效果与疼痛模型有关。 | Kinga SALAT Barbara FILIPEK | 2015 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2015，16，3： | 5 |
| 4 | Levels and activities of von Willebrand factor and metalloproteinase with thrombospondin type-1 motif, number 13 in inflammatory bowel diseases显示文摘AIM To evaluate the levels of von Willebrand factor(VWF) and metalloproteinase with thrombospondin type-1 motif, number 13(ADAMTS13) in inflammatory bowel disease(IBD) and correlate them with the disease activity.METHODS Consecutive patients with IBD aged 18 years or older were enrolled in the study. Forty-seven patients with ulcerative colitis(UC), 38 with Crohn's disease(CD), and 50 healthy controls were included. The white blood cell count, haematocrit, platelet count, fibrinogen, partial activated thromboplastin time, C-reactive protein, albumin, VWF antigen level(VWF:Ag), VWF ristocetin cofactor activity(VWF:RCo), VWF collagen-binding activity(VWF:CB), and ADAMTS13 antigen level(ADAMTS13:Ag) and activity(ADAMTS13act) were measured. The following ratios were assessed: V W F : R C o/V W F : A g, V W F : C B/V W F : A g, V W F : A g/ADAMTS13 act, and ADAMTS13act/ADAMTS13:Ag. RESULTS Compared to controls, the odds ratio(OR) of an elevated VWF: Ag > 150% was 8.7(95%CI: 2.7-28.1) in the UC group and 16.2(95%CI: 4.8-54.0) in the CD group. VWF:CB was lower in UC patients, and active CD was associated with a higher VWF: RCo(+38%). The ORs of VWF:CB/VWF:Ag < 0.7(a marker of acquired von Willebrand syndrome) in the UC and CD groups were 11.9(95%CI: 4.4-32.4) and 13.3(95%CI: 4.6-38.1), respectively. Active UC was associated with lower ADAMTS13:Ag(-23%) and ADAMTS13act(-20%) compared to UC in remission. Patients with active CD had a 15% lower ADAMTS13 act than controls. The activity of UC, but not that of CD, was inversely correlated with ADAMTS13:Ag(r =-0.76) and ADAMTS13act(r =-0.81). CONCLUSION Complex VWF-ADAMTS13-mediated mechanisms disturb haemostasis in IBD. A reduced WVF:CB is a risk factor for bleeding, while a lower ADAMTS13 level combined with an elevated VWF:Ag could predispose one to thrombosis. | Dorota Cibor Danuta Owczarek Saulius Butenas Kinga Salapa Tomasz Mach Anetta Undas | 2017 | World Journal of Gastroenterology2017，23，26： | 3 |
| 5 | IL23R single nucleotide polymorphisms could be either beneficial or harmful in ulcerative colitis显示文摘AIM To investigate the association of seven single nucleotide polymorphisms(SNPs) of the IL23 R gene with the clinical picture of ulcerative colitis(UC). METHODS Genomic DNA samples of 131 patients (66 males, 65 females, mean age 55.4 ± 15.8 years) with Caucasian origin, diagnosed with UC were investigated. The diagnosis of UC was based on the established clinical, endoscopic, radiological, and histopathological guidelines. DNA was extracted from peripheral blood leukocytes by routine salting out method. Polymerase chain reaction and restriction fragment length polymorphism were used to identify the alleles of seven SNPs of IL23 R gene(rs11209026, rs10889677, rs1004819, rs2201841, rs7517847, rs10489629, rs7530511).RESULTS Four out of seven analyzed SNPs had statistically significant influence on the clinical picture of UC. Two SNPs were associated with greater colonic extension(rs2201841 P = 0.0084; rs10489629 P = 0.0405). For two of the SNPs, there was more frequently need for operations (rs2201841 P = 0.0348, OR = 8.0; rs10889677 P = 0.0347, OR = 8.0). The rs2201841 showed to be a risk factor for the development of iron deficiency (P = 0.0388, OR = 6.1837). For patients with the rs10889677, a therapy with azathioprine was more frequently necessary(P = 0.0116, OR = 6.1707). Patients with rs10489629 SNP had a lower risk for weight loss(P = 0.0169, OR = 0.3394). Carriers of the heterozygous variant had a higher risk for an extended disease (P = 0.0284). The rs7517847 showed a protective character leading to mild bowel movements. Three SNPs demonstrated no statistically significant influence on any examined clinical features of UC.CONCLUSION We demonstrated susceptible or protective character of the investigated IL23 R SNPs on the phenotype of UC, confirming the genetic association. | Sarah Fischer Erzsébet Kovesdi Lili Magyari Veronika Csongei Kinga Hadzsiev Béla Melegh Péter Hegyi Patrícia Sarlós | 2017 | World Journal of Gastroenterology2017，23，3： | 3 |
| 6 | The immunomodulating enteral nutrition in malnourished surgical patients – A prospective, randomized, double-blind clinical trial显示文摘 | Stanislaw Klek Marek Sierzega Piotr Szybinski Kinga Szczepanek Lucyna Scislo Elzbieta Walewska Jan Kulig | 2010 | Clinical Nutrition2010，，3： | 2 |
| 7 | Cells and material-based strategies for regenerative endodontics显示文摘 The carious process leads to inflammation of pulp tissue.Current care options include root canal treatment or apexification.These procedures,however,result in the loss of tooth vitality,sensitivity,and healing.Pulp capping and dental pulp regeneration are continually evolving techniques to regenerate pulp tissue,avoiding necrosis and loss of vitality.Many studies have successfully employed stem/progenitor cell populations,revascularization approaches,scaffolds or material-based strategies for pulp regeneration.Here we outline advantages and disadvantages of different methods and techniques which are currently being used in the field of regenerative endodontics.We also summarize recent findings on efficacious peptide-based materials which target the dental niche.. | Zain Siddiqui Amanda M.Acevedo-Jake Alexandra Griffith Nurten Kadincesme Kinga Dabek Dana Hindi Ka Kyung Kim Yoshifumi Kobayashi Emi Shimizu Vivek Kumar | 2022 | Bioactive Materials2022，7，8： | 2 |
| 8 | Trends in NO x abatement: A review显示文摘 | Kinga Skalska Jacek S. Miller Stanislaw Ledakowicz | 2010 | Science of the Total Environment2010，，19： | 2 |
| 9 | Down-Regulation of P27 is associated with development of colorectal adenocarcinoma metastases显示文摘 | Georeg V Kinga s Michael M | 1998 | Am J Pathol1998，153，3： | 1 |
| 10 | Knowledge about cervical cancer among Polish and Finnish female students显示文摘 | Kinga Kuczkowska Kamila Jankowiak-Siuda Zbigniew Wronkowski | 2011 | European Journal of Obstetrics and Gynecology2011，，2： | 1 |
| 11 | Influence of melatonin upon the protective action of conventionsl anti-epileptic drugs against maximal electroshock in mice显示文摘 | Kinga K Borowicz RK Maciej G | 1999 | Europ Neuropsychopharmacol1999，9，： | 1 |
| 12 | Recent developments in polymeric phase change materials for energy storage: poly(ethylene oxide)/ stearic acid blends显示文摘 | Krzysztof Pielichowski Kinga Flejtuch | 2005 | Polymers for Advanced Technologies2005，16，23： | 1 |
| 13 | Oral administration of Aloe vera and honey reduces walker tumour growth by decreasing cell proliferation and increasing apoptosis in tumour tissue显示文摘 | Robert T Kinga P Simone G | 2011 | Neopla- sia2011，25，： | 1 |
| 14 | Is interview a reliable method to verify the compliance with antihypertensive therapy? ?,n international central-European study 显示文摘 | George JF Marian K Kinga B | 2005 | Journal of Hypertension2005，23，： | 1 |
| 15 | Five novel RPGR mutations in families with X-linked retinitis pigmentosa显示文摘 | STACEY F KINGA B | 2001 | Hum Mutat2001，17，2： | 1 |
| 16 | Zeta chain expression in T and NK cells in peripheral tlood of children with nephrotic syndrome 显示文摘 | Kinga M Lidia E Agata Kosmaczewska | 2010 | Pediatr Nephrol2010，25，1： | 1 |
| 17 | Molecular diagnosis Polymor-phisms of tumor necrosis factor and myeloperoxidasegenes in patients with chronic renal failure 显示文摘 | Buraczynska Kinga/k | 2009 | Peritoneal dialysis2009，7，3： | 1 |
| 18 | Apical and rootcanal space sealing abilities of resin and glass ionomerbasedroot canal obturation systems显示文摘 | Royer Kinga Liu Xue Jun Zhu Qiang | 2013 | The Chinese journalof dental research:the official journal of the ScientificSection of the Chinese Stomatological Association (CSA)2013，16，1： | 1 |
| 19 | Proto colsand architectrure formanaging TCP/IP network infrastructures显示文摘 | HuntbR | 2000 | Computer Communications2000，，： | 1 |
| 20 | 使用口腔液对后备猪群进行蓝耳病病毒驯化显示文摘一个健康发展的猪场每年都保持30%~50%的引种率。引种既是维持繁殖猪群数量稳定和胎次结构科学分布、保证猪场生产成绩必不可少的环节,又是充满风险的环节。一旦引种后处理不当,则可能导致疾病的暴发,而科学的隔离和驯化则是降低疾病暴发风险的关键环节。以蓝耳病病毒驯化为例,驯化的目标是让同一批后备猪同期感染本场流行的蓝耳病病毒,在并群前既要产生针对性的免疫保护、又要结束蓝耳病病毒的排毒。为了尽可能实现驯化的目标,本文介绍了一种非常实用的后备猪驯化方法,该方法基于用棉绳收集病毒血症期猪群口腔液,随后将棉绳悬挂于后备猪栏,让后备猪感染棉绳中的蓝耳病病毒而实现驯化。本方法适用于大、中、小各种类型的猪场,为实现稳定猪场的蓝耳病、改善生产指标并在断奶时获得蓝耳病病毒阴性仔猪增加了可能。 | 刘远佳 郑国超 Piotr Biernacki Kinga Biernacka | 2017 | 中国猪业2017，12，9： | 1 |
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