| 1 | Neither gastric topological distribution nor principle virulence genes of Helicobacter pylori contributes to clinical outcomes显示文摘AIM: Studies on Helicobacter pylori (H pylon) and gastroduodenal diseases have focused mainly on the distal sites of the stomach, but relationship with the gastric cardia is lacking. The aim of this study is to determine if the gastric topology and genotypic distribution of Hpyloriwere associated with different upper gastrointestinal pathologies in a multiethnic Asian population. METHODS: Gastric biopsies from the cardia, body/corpus and antrum were endoscoped from a total of 155 patients with dyspepsia and/or reflux symptoms, with informed consent. H pylori isolates obtained were tested for the presence of 26kDa, ureC, cagA, vacA, iceA1, iceA2 and babA2 genes using PCR while DNA fingerprints were generated using random amplification polymorphic DNA (RAPD). RESULTS: Hpyloriwas present in 51/155 (33%) of patients studied. Of these, 16, 15 and 20 were isolated from patients with peptic ulcer diseases, gastroesophageal reflux diseases and non-ulcer dyspepsia, respectively. Of the Hpyloripositive patients, 75% (38/51) had Hpyloriin all three gastric sites. The prevalence of various genes in the H pylori isolates was shown to be similar irrespective of their colonization sites as well as among the same site of different patients. The RAPD profiles of H pylori isolates from different gastric sites were highly similar among intra-patients but varied greatly between different patients. CONCLUSION: Topographic colonization of H pylori and the virulence genes harboured by these isolates have no direct bearing to the clinical state of the patients. In multiethnic Singapore, the stomach of each patient is colonized by a predominant strain of H pylori,irrespective of the clinical diagnosis. | YanWingHo KhekYuHo FelipeAscencio BowHo | 2004 | World Journal of Gastroenterology2004，10，22： | 5 |
| 2 | Effect of biopsies on sensitivity and specificity of ultra-rapid urease test for detection of Helicobacter pylori infection:A prospective evaluation显示文摘AIM: To prospectively assess the sensitivity, specificity and time to positivity of theUItra-rapid urease test (URUT) for Helicobacter pylori ( H pylori ), and compare the results of one with those of two biopsies.METHODS: Five antral biopsies were taken in consecutive patients undergoing upper endoscopy: one and two biopsies for URUT, and one each for H pylori culture and histology.URUT was read at 1, 5, 10, 20 and 30 rain, 1, 2, 3 and 24 h after biopsy insertion into the reagent. A positive histology and/or culture was used as positive reference “gold standards”:RESULTS: URUT was more sensitive for detecting Hpy/ori with two biopsies rather than one, at all time points up to 120 rain. The sensitivity improved from 3.6% to 82.1% for one biopsy and 10.7% to 85.7% for two biopsies from 1 to 120 rain. The sensitivity reached 96.4% at 24 h for both,but the specificity reduced from 100% to 96% and 92% for one and two biopsies, respectively.CONCLUSION: Development of a positive URUT result is hastened by doubling the number of gastric biopsies. We recommend taking two instead of one biopsy to achieve an earlier positive URUT result so that H pylorieradication therapy can be initiated before patient is discharged from the endoscopy suite. | LiLinLim KhekYuHo Bow Ho Manuel Salto-Tellez | 2004 | World Journal of Gastroenterology2004，10，13： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员