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| 1 | Regulation of extracellular bioactive cations in bone tissue microenvironment induces favorable osteoimmune conditions to accelerate in situ bone regeneration显示文摘The design of orthopedic biomaterials has gradually shifted from“immune-friendly”to“immunomodulatory,”in which the biomaterials are able to modulate the inflammatory response via macrophage polarization in a local immune microenvironment that favors osteogenesis and implant-to-bone osseointegration.Despite the well-known effects of bioactive metallic ions on osteogenesis,how extracellular metallic ions manipulate immune cells in bone tissue microenvironments toward osteogenesis and subsequent bone formation has rarely been studied.Herein,we investigate the osteoimmunomodulatory effect of an extracellular bioactive cation(Mg^(2+))in the bone tissue microenvironment using custom-made poly lactic-co-glycolic acid(PLGA)/MgO-alendronate microspheres that endow controllable release of magnesium ions.The results suggest that the Mg^(2+)-controlled tissue microenvironment can effectively induce macrophage polarization from the M0 to M2 phenotype via the enhancement of anti-inflammatory(IL-10)and pro-osteogenic(BMP-2 and TGF-β1)cytokines production.It also generates a favorable osteoimmune microenvironment that facilitates the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.The in vivo results further verify that a large amount of bony tissue,with comparable bone mineral density and mechanical properties,has been generated at an early post-surgical stage in rat intramedullary bone defect models.This study demonstrates that the concept of in situ immunomodulated osteogenesis can be realized in a controlled magnesium tissue microenvironment. | Zhengjie Lin Danni Shen Weixiao Zhou Yufeng Zheng Tiantian Kong Xuanyong Liu Shuilin Wu Paul K.Chu Ying Zhao Jun Wu Kenneth M.C.Cheung Kelvin W.K.Yeung | 2021 | Bioactive Materials2021，6，8： | 5 |
| 2 | 遗传性骨软骨瘤软骨细胞的生物学特性显示文摘目的:观察遗传性骨软骨瘤软骨细胞的生物学特性。方法:采用透射电镜、细胞培养等方法观察肿瘤软骨细胞形态特征;同时观察肿瘤软骨细胞的增殖、贴附能力,与正常人关节软骨细胞做对照。结果:透射电镜(TEM)发现肿瘤软骨细胞膜附近含有大量的微丝结构,集结成束,与细胞突起有关;体外单层培养发现肿瘤软骨细胞突起增多,细胞呈现星形;骨软骨瘤软骨细胞的增殖与贴附能力随着传代的增加减慢和减低,传4代细胞增殖能力和贴附能力均与肿瘤原代细胞及正常关节软骨细胞具有明显差别(P<0.1)。结论:骨软骨瘤软骨细胞虽然与正常软骨细胞大体相似,但生物学特性存在明显区别:细胞形态改变,微丝增加,细胞增殖与贴附能力等均不同于正常软骨细胞。 | 杨柳 罗卓荆 颉强 靳小兵 Wing-Sum Hui Danny Chan Jian-Dong Huang Kenneth M.C.Cheung Kathryn S.E.Cheah | 2003 | 中国矫形外科杂志2003，11，22： | 3 |
| 3 | Engineered polycaprolactone–magnesium hybrid biodegradable porous scaffold for bone tissue engineering显示文摘In this paper, we describe the fabrication of a new biodegradable porous scaffold composed of polycaprolactone(PCL) and magnesium(Mg)micro-particles. The compressive modulus of PCL porous scaffold was increased to at least 150% by incorporating 29% Mg particles with the porosity of 74% using Micro-CT analysis. Surprisingly, the compressive modulus of this scaffold was further increased to at least 236% when the silane-coupled Mg particles were added. In terms of cell viability, the scaffold modified with Mg particles significantly convinced the attachment and growth of osteoblasts as compared with the pure PCL scaffold. In addition, the hybrid scaffold was able to attract the formation of apatite layer over its surface after 7 days of immersion in normal culture medium, whereas it was not observed on the pure PCL scaffold. This in vitro result indicated the enhanced bioactivity of the modified scaffold. Moreover, enhanced bone forming ability was also observed in the rat model after 3 months of implantation. Though bony in-growth was found in all the implanted scaffolds. High volume of new bone formation could be found in the Mg/PCL hybrid scaffolds when compared to the pure PCL scaffold. Both pure PCL and Mg/PCL hybrid scaffolds were degraded after 3 months. However, no tissue inflammation was observed. In conclusion, these promising results suggested that the incorporation of Mg micro-particles into PCL porous scaffold could significantly enhance its mechanical and biological properties. This modified porous bio-scaffold may potentially apply in the surgical management of large bone defect fixation. | Hoi Man Wong Paul K.Chu Frankie K.L.Leung Kenneth M.C.Cheung Keith D.K.Luk Kelvin W.K.Yeung | 2014 | Progress in Natural Science:Materials International2014，24，5： | 3 |
| 4 | Stepwise 3D-spatio-temporal magnesium cationic niche: Nanocomposite scaffold mediated microenvironment for modulating intramembranous ossification显示文摘The fate of cells and subsequent bone regeneration is highly correlated with temporospatial coordination of chemical,biological,or physical cues within a local tissue microenvironment.Deeper understanding of how mammalian cells react to local tissue microenvironment is paramount important when designing next generation of biomaterials for tissue engineering.This study aims to investigate that the regulation of magnesium cationic(Mg^2+)tissue microenvironment is able to convince early-stage bone regeneration and its mechanism undergoes intramembranous ossification.It was discovered that moderate Mg^2+content niche(~4.11 mM)led to superior bone regeneration,while Mg^2+-free and strong Mg^2+content(~16.44 mM)discouraged cell adhesion,proliferation and osteogenic differentiation,thereby bone formation was rarely found.When magnesium ions diffused into free Mg zone from concentrated zone in late time point,new bone formation on free Mg zone became significant through intramembranous ossification.This study successfully demonstrates that magnesium cationic microenvironment serves as an effective biochemical cue and is able to modulate the process of bony tissue regeneration.The knowledge of how a Mg^2+cationic microenvironment intertwines with cells and subsequent bone formation gained from this study may provide a new insight to develop the next generation of tissuerepairing biomaterials. | Jie Shen Bo Chen Xinyun Zhai Wei Qiao Shuilin Wu Xuanyong Liu Ying Zhao Changshun Ruan Haobo Pan Paul K.Chu Kenneth M.C.Cheung Kelvin W.K.Yeung | 2021 | Bioactive Materials2021，6，2： | 2 |
| 5 | Magnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone显示文摘Regardless of the advancement of synthetic bone substitutes,allograft-derived bone substitutes still dominate in the orthopaedic circle in the treatments of bone diseases.Nevertheless,the stringent devitalization process jeopardizes their osseointegration with host bone and therefore prone to long-term failure.Hence,improving osseointegration and transplantation efficiency remains important.The alteration of bone tissue microenvironment(TME)to facilitate osseointegration has been generally recognized.However,the concept of exerting metal ionic cue in bone TME without compromising the mechanical properties of bone allograft is challenging.To address this concern,an interfacial tissue microenvironment with magnesium cationc cue was tailored onto the gamma-irradiated allograft bone using a customized magnesium-plasma surface treatment.The formation of the Mg cationic cue enriched interfacial tissue microenvironment on allograft bone was verified by the scanning ion-selective electrode technique.The cellular activities of human TERT-immortalized mesenchymal stem cells on the Mg-enriched grafts were notably upregulated.In the animal test,superior osseointegration between Mg-enriched graft and host bone was found,whereas poor integration was observed in the gamma-irradiated controls at 28 days post-operation.Furthermore,the bony in-growth appeared on magnesium-enriched allograft bone was significant higher.The mechanism possibly correlates to the up-regulation of integrin receptors in mesenchymal stem cells under modified bone TME that directly orchestrate the initial cell attachment and osteogenic differentiation of mesenchymal stem cells.Lastly,our findings demonstrate the significance of magnesium cation modified bone allograft that can potentially translate to various orthopaedic procedures requiring bone augmentation. | Wenhao Wang Jie Shen Yuan Meng Miaoman Ye Shaozhang Lin Qi Zhao Le Wang Kenneth M.C.Cheung Shuilin Wu Yufeng Zheng Xuanyong Liu Paul K.Chu Kelvin W.K.Yeung Zhi-Yong Zhang | 2022 | Bioactive Materials2022，7，4： | 0 |
| 6 | 遗传性骨软骨瘤软骨细胞的体外培养显示文摘目的:通过对人遗传性骨软骨瘤软骨细胞的体外培养,观察肿瘤软骨细胞的生长特性及相关生物学特点,为进一步研究骨软骨瘤细胞学起因奠定基础。方法:原代培养遗传性骨软骨瘤组织中的软骨细胞并进行传代,进行活细胞形态观察,同时测定细胞增殖能力及贴壁率,与正常人关节软骨细胞做对照。结果:原代细胞在经过完全消化后,细胞活力仍达到95%。传代后骨软骨瘤细胞体积增大,细胞呈现星形,树突增多,细胞内颗粒多且明显。骨软骨瘤细胞增殖随着传代的增加减慢,第2代细胞增殖能力已经与原代细胞产生明显差别(P<0.01,t=3.203)。原代骨软骨瘤细胞在24h内大部分贴壁,随着传代的增加,细胞贴附能力下降,第4代细胞贴壁率与原代细胞产生明显区别(P<0.01,t=4.611)。结论:体外培养的骨软骨瘤细胞从形态,生物学特性(细胞增殖情况、细胞贴附能力)均不同于正常软骨细胞,为今后更加深入的研究遗传骨软骨瘤的病因学奠定一定的体外培养基础。 | 杨柳 罗卓荆 颉强 靳小兵 Danny Chan Wing-Sum Hui Jian-Dong Huang Kenneth M.C.Cheung Kathryn S.E.Cheah | 2003 | 中国临床康复2003，7，26： | 0 |
| 7 | Corrigendum to“Magnesium cationic cue enriched interfacial tissue microenvironment nurtures the osseointegration of gamma-irradiated allograft bone”[Bioact.Mater.10C(April 2022)32-47]显示文摘The authors regret a mistake of funding numbers in the Acknowledgment Section failed to be corrected during proof reading.Below is the corrected funding statement in Acknowledgment SECTION This work was supported by the National Natural Science Foundation of China(NSFC)(Nos.81902189,81772354,82002303,31570980),Clinical Innovation Research Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory(2018GZR0201002),National Key Research and Development Plan(2018YFC1105103). | Wenhao Wang Jie Shen Yuan Meng Miaoman Ye Shaozhang Lin Qi Zhao Le Wang Kenneth M.C.Cheung Shuilin Wu Yufeng Zheng Xuanyong Liu Paul K.Chu Kelvin W.K.Yeung Zhi-Yong Zhang | 2023 | Bioactive Materials2023，，2： | 0 |
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