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| 1 | Selective feeding of bay scallop Argopecten irradians on phytoplankton community revealed by HPLC analysis of phytopigments in Bohai Sea, China显示文摘Understanding the feeding selectivity on phytoplankton by shellfi sh is currently a big challenge. In order to investigate the feeding behavior of bay scallop ( Argopecten irradians ) on phytoplankton, we compared its compositions of phytopigments in digestive glands with those in the surrounding seawater, and conducted fi ve consecutive investigations between July and November 2016 in a bay scallop culture area along coast of Qinghuangdao City, northwest of the Bohai Sea, China. Phytopigments in four-size fractionated phytoplankton of seawater (micro-(20-200 μm);nano(L)-[10-20 μm];nano(S)-[2.7-10 μm], and pico-[<2.7 μm]) and digestive glands of A . irradians were examined to investigate the selective feeding of A . irradians . Results show that fucoxanthin and peridinin constituted the major part of taxonomically diagnostic carotenoids (TDCs) in the micro- and nano(L)-phytoplankton in seawater. Compared with total phytoplankton biomass of seawater (TPB, sum of the four sizes), a substantial decrease of fucoxanthin proportion to total DCs in digestive glands was observed while that of peridinin, 19′-butanoyloxyfucoxanthin, alloxanthin and 19′-hexanoyloxy-fucoxanthin showed an obvious increase when those pigments were mainly confi ned to micro-sized phytoplankton (20-200 μm). However, zeaxanthin and prasinoxanthin were mainly confi ned to nano(s)- and pico-phytoplankton, of which the proportions in digestive glands were usually lower in TPB. The contribution of lutein to total DCs in digestive glands (with an average of 7.23%) increased compared with TPB of seawater (with an average of 0.63%) during all fi ve sampling times. | JIANG Tao WANG Longhua ZHANG Fuchong FANG Xiao LU Lin ZHANG Jihong WANG Wei QU Keming CHAI Chao | 2019 | Journal of Oceanology and Limnology2019，37，5： | 4 |
| 2 | Quantifying the characteristics of particulate matters captured by urban plants using an automatic approach显示文摘It is widely accepted that urban plant leaves can capture airborne particles. Previous studies on the particle capture capacity of plant leaves have mostly focused on particle mass and/or size distribution. Fewer studies, however, have examined the particle density, and the size and shape characteristics of particles, which may have important implications for evaluating the particle capture efficiency of plants, and identifying the particle sources. In addition, the role of different vegetation types is as yet unclear. Here, we chose three species of different vegetation types, and firstly applied an object-based classification approach to automatically identify the particles from scanning electron microscope(SEM)micrographs. We then quantified the particle capture efficiency, and the major sources of particles were identified. We found(1) Rosa xanthina Lindl(shrub species) had greater retention efficiency than Broussonetia papyrifera(broadleaf species) and Pinus bungeana Zucc.(coniferous species), in terms of particle number and particle area cover.(2) 97.9% of the identified particles had diameter ≤10 μm, and 67.1% of them had diameter ≤2.5 μm. 89.8% of the particles had smooth boundaries, with 23.4% of them being nearly spherical.(3) 32.4%–74.1% of the particles were generated from bare soil and construction activities, and 15.5%–23.0% were mainly from vehicle exhaust and cooking fumes. | Jingli Yan Lin Lin Weiqi Zhou Lijian Han Keming Ma | 2016 | Journal of Environmental Sciences2016，28，1： | 3 |
| 3 | Aquaporin-1 Expressed in Cultured Human Trabecular Meshwork Cells显示文摘Objective:To determine if aquaporin-1 could be detected in cultures of human trabecularshwork cells. Methods: Using primers specific for aquaporin-1, reverse transcription combined withpolymerase chain reaction (RT-PCR) yielded a product and its size with total RNAprepared from the human trabecular meshwork cells. SDS-PAGE and immunoblottingwere also used in this study to detect the specific water channel.Results: The presence of this product and its size (298 base pairs) are consistent withthat of an aquaporin-1 message in these cells. A band of 28 kD in agreement with themolecular size of aquaporin-1 was showed in a film by immunoblotting.Conclusion: The presence of aquaporin-1 in human trabecular meshwork cells, thepredominant cell-type of the primary outflow region of the human eye, suggests that waterchannels may be involved in the movement of aqueous fluid out of the eye. In addition,the existence of aquaporin-1 on cultures of human trabecular meshwork cells provides anin vitro model to study the endogenous expression of aquaporin-1 and its possible role inthe regulation of aqueous outflow. | Mingkai Lin, Jian Ge, Yehong Zhuo, Yuqing Lan, Keming Yu, Jianliang ZhengZhongshan Ophthalmic center, Sun Yat-sen University, Guangzhou 510060, China | 2002 | Eye Science2002，18，1X： | 2 |
| 4 | KIFC1 participates in acrosomal biogenesis, with discussion of its importance for the perfo ratorium in the Chinese mitten crab Eriocheir sinensis 显示文摘 | Yu Keming Hou Lin Zhu Junquan | 2009 | Cell Tissue Res2009，337，1： | 1 |
| 5 | Extraction and antioxidant activities of intracellular polysaccharide from Pleurotus sp. mycelium显示文摘 | Xiaonan Liu Bo Zhou Rongshan Lin Le Jia Peng Deng Keming Fan Guoyi Wang Li Wang Jianjun Zhang | 2010 | International Journal of Biological Macromolecules2010，，2： | 1 |
| 6 | Effects of road dust on the growth characteristics of Sophora japonica L.seedlings显示文摘Road dust is one of the most common pollutants and causes a series of negative effects on plant physiology. Dust's impacts on plants can be regarded as a combination of load,composition and grain size impacts on plants; however, there is a lack of integrated dust effect studies involving these three aspects. In our study, Sophora japonica seedlings were artificially dusted with road dust collected from the road surface of Beijing so that we could study the impacts of this dust on nitrogen/carbon allocation, biomass allocation and photosynthetic pigments from the three aspects of composition, load and grain size. The results showed that the growth characteristics of S. japonica seedlings were mostly influenced by dust composition and load. Leaf N, root–shoot ratio and chlorophyll a/b were significantly affected by dust composition and load; leaf C/N, shoot biomass, total chlorophyll and carotenoid were significantly affected by dust load; stem N and stem C/N were significantly affected by dust composition; while the dust grain size alone did not affect any of the growth characteristics. Road dust did influence the growth characteristics more extensively than loam. Therefore, a higher dust load could increase the differences between road dust and loam treatments. The elements in dust are well correlated to the shoot N, shoot C/N, and root–shoot ratio of S. japonica seedlings. This knowledge could benefit the management of urban green spaces. | Le Bao Laiye Qu Keming Ma Lin Lin | 2016 | Journal of Environmental Sciences2016，28，8： | 1 |
| 7 | Production and in vitro antioxidant activity of exopolysaccharide by a mutant, Cordyceps militaris SU5-08显示文摘 | Rongshan Lin Honghong Liu Suqian Wu Lifei Pang Mengshi Jia Keming Fan Shouhua Jia Le Jia | 2012 | International Journal of Biological Macromolecules (-)2012，，1： | 1 |
| 8 | Woody plant cell walls:Fundamentals and utilization显示文摘Cell walls in plants,particularly forest trees,are the major carbon sink of the terrestrial ecosystem.Chemical and biosynthetic features of plant cell walls were revealed early on,focusing mostly on herbaceous model species.Recent developments in genomics,transcriptomics,epigenomics,transgenesis,and associated analytical techniques are enabling novel insights into formation of woody cell walls.Here,we review multilevel regulation of cell wall biosynthesis in forest tree species.We highlight current approaches to engineering cell walls as potential feedstock for materials and energy and survey reported field tests of such engineered transgenic trees.We outline opportunities and challenges in future research to better understand cell type biogenesis for more efficient wood cell wall modification and utilization for biomaterials or for enhanced carbon capture and storage. | Wei Li Ying-Chung Jimmy Lin Ying-Lan Chen Chenguang Zhou Shuang Li Nette De Ridder Dyoni M.Oliveira Lanjun Zhange Baocai Zhang Jack P.Wang Changzheng Xu Xiaokang Fu Keming Luo Ai-Min Wu Taku Demura Meng-Zhu Lu Yihua Zhou Laigeng Li Toshiaki Umezawa Wout Boerjan Vincent L.Chiang | 2024 | Molecular Plant2024，17，1： | 0 |
| 9 | Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins显示文摘Background:Existing treatments for cholangiocarcinoma have poor efficacy.However,chimeric antigen receptor-T(CAR-T)cells are emerging as a potential therapeutic strategy.Solid tumors possess multiple adverse factors in an immunosuppressive microenvironment that impair CAR-T cell infiltration and function.This study aimed to improve the function of CAR-T cells through knock down immune checkpoints and immunosuppressive molecular receptors.Methods:We evaluated the expression of epidermal growth factor receptor(EGFR)and B7 homolog 3 protein(B7H3)antigens in cholangiocarcinoma tissues using immunohistochemistry and screened specific immune checkpoints in the cholangiocarcinoma microenvironment via flow cytometry.Subsequently,we engineered CAR-T cells targeting EGFR and B7H3 antigens.We simultaneously knocked down immune checkpoints and immunosuppressive molecular receptors in CAR-T cells by constructing two clusters of small hairpin RNAs and evaluated the engineered CAR-T cells for antitumor activity both in vitro,using tumor cell lines and cholangiocarcinoma organoid models,and in vivo,using humanized mouse models.Results:We observed high expression of EGFR and B7H3 antigens in cholangiocarcinoma tissues.EGFR-CAR-T and B7H3-CAR-T cells demonstrated specific anti-tumor activity.We found an abundance of programmed cell death protein 1(PD-1),T cell immunoglobulin and mucin domain-containing protein 3(Tim-3),and T cell immunoglobulin and ITIM domain(Tigit)on infiltrated CD8^(+)T cells in the cholangiocarcinoma microenvironment.We then decreased the expression of these 3 proteins on the surface of CAR-T cells,named PTG-scFV-CAR-T cells.Furthermore,we knocked-down the expression of transforming growth factor beta receptor(TGFβR),interleukin-10 receptor(IL-10R),and interleukin-6 receptor(IL-6R)of PTG-scFV-CAR-T cells.Those cells,named PTG-T16R-scFVCAR-T cells,potently killed tumor cells in vitro and promoted apoptosis of tumor cells in a cholangiocarcinoma organoidmodel.Finally,the PTG-T16R-scFv-CART cells showed greater inhibitory effect on tumor growth in vivo,and were superior in prolonging the survival of mice.Conclusions:Our results revealed that PTG-T16R-scFV-CAR-T cells with knockdown of sextuplet inhibitory molecules exhibited strong immunity against cholangiocarcinoma and long-term efficacy both in vitro and in vivo.This strategy provides an effective and personalized immune cell therapy against cholangiocarcinoma. | Yidan Qiao Jie Chen Xuemei Wang Shumei Yan Jizhou Tan Baijin Xia Yongjian Chen Keming Lin Fan Zou Bingfeng Liu Xin He Yiwen Zhang Xu Zhang Hui Zhang Xiangyuan Wu Lijuan Lu | 2023 | Cancer Communications2023，43，7： | 0 |
| 10 | Expert Consensus on Nutritional Support for Children with Congenital Heart Disease(2023 Edition)显示文摘The second edition of the expert consensus on pediatric nutrition was formed based on a global update of pedia-tric nutrition guidelines or consensus worldwide,the management of congenital heart disease,and the results of multi-center clinical nutrition research for congenital heart disease following thefirst Chinese consensus edition of 2016.The consensus was also shaped by the results of three discussion sessions and two questionnaires con-ducted by the 13-member collaboration group.This process was informed by both clinical guidelines and expert consensus.The quality of literature,both in English and Chinese,and the level of recommendations were evaluated using the Grading of Recommendations Assessment,Development,and Evaluations(GRADE)system. | Xuming Mo Wei Cai Jirong Qi Zhuoming Xu Ying Wang Weihui Yan Shoujun Li Nianguo Dong Xinxin Chen Jinfen Liu Qiang Shu Jimei Chen Haibo Zhang Hao Zhang Quansheng Xing Qi An Xiaofeng Li Xu Wang Yan He Junwu Su Taibing Fan Teng Ming Weibing Tang Li Hong Jinghao Zheng Ming Ye Guocheng Sun Yiqun Ding Liang Tao Yifeng Yang Zhongshi Wu Hua Cao Qiang Wang Keming Yang Libing Zhang Ping Wen Yanqin Cui Bo Zhai Yong Zou Qingya Tang Rui Chen Chun Wu Zhiyu Feng Caixia Liu Yaping Mi Rufang Zhang Ke Lin Xin Li Mingan Pi Xiangming Fan Shanshan Shi Peng Huang Zhengxia Pan Jiafeng Qi Renwei Chen Shuguang Tao Yaqin Shu Huifeng Zhang Lan Jiang Min Da Nishant Patel Liang Hu Cardiac Surgery Group of Pediatric Surgery Society of Chinese Medical Association and Parenteral Enteral Nutrition Society of Chinese Medical Association | 2023 | Congenital Heart Disease2023，18，6： | 0 |
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