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| 1 | Variation in needle and cone traits in natural populations of Pinus yunnanensis显示文摘Pinus yunnanensis Franch. is a major component of coniferous forests in southwestern China. Little is known about its intraspecific variation. Morphological variations in needle and cone traits of P. yunnanensis were analyzed to quantify variability among and within natural populations.Seven traits were measured on 10 needles collected from 30 trees in each of the 18 sampled populations of P. yunnanensis. Four cone traits were measured in 221 individual trees from nine populations. The results showed that there were significant differences(p \ 0.01) both among populations and within populations in each needle and cone trait.The proportion of phenotypic variation of nearly all needle and cone traits was over 50 % within populations, which showed trees within populations accounted for a majority of the total variation. The needle traits showed higher variability within population than cone traits. Variability in theneedle traits was correlated with geo-climatic parameters(longitude, latitude, altitude, temperature, and precipitation).Needle length and the ratio of needle length to fascicle sheath length showed clinal variation in response to latitudinal and altitudinal gradients. A hierarchical classification of all populations based on needle traits led to the formation of four major groups. The findings provide important genetic information for the evaluation of variation. Moreover, it will assist in management of genetic diversity of P. yunnanensis. | Yulan Xu Keith Woeste Nianhui Cai Xiangyang Kang Genqian Li Shi Chen Anan Duan | 2016 | Journal of Forestry Research2016，27，1： | 15 |
| 2 | Germination and early seedling growth of Pinus densata Mast.provenances显示文摘We studied seed germination and early seedling growth of Pinus densata to explore the range of variability within the species and to inform afforestation practices.Phenotypes were evaluated at a forest tree nursery under conditions that support Pinus yunnanensis, one of the presumed parental species of P. densata. Seeds were collected from 20 open-pollinated trees within each of eight autochthonous populations representing the natural distribution of P. densata in China to assess variation in germination traits and early seedling growth, and to examine the relationships among these traits. Results showed that seeds from all populations germinated and seedlings established successfully. There were significant differences among populations in 13 of 14 traits evaluated. Seed germination and early seedling growth were strongly related to seed size and seed weight. Bigger seeds germinated earlier and faster than small seeds, and seedling size was positively correlated with seed size. Some germination traits were strongly and significantly correlated with climatic variables associated with the provenance of the studied populations. Based on these observations, we conclude there were large, significant, and biologically important differences among P. densata populations in seed germination and seedling growth traits. The observed variability probably reflects a high degree of adaptive differentiation among populations that is likely to be relevant for future afforestation. | Yulan Xu Nianhui Cai Bin He Ruili Zhang Wei Zhao Jianfeng Mao Anan Duan Yue Li Keith Woeste | 2016 | Journal of Forestry Research2016，27，2： | 5 |
| 3 | 胡桃属植物分子系统发育和生物地理研究进展显示文摘胡桃属植物约有21种,在亚洲、欧洲和美洲均有分布,为间断分布类群。该属植物种质资源丰富,栽培历史久,具有重要的生物学意义和经济价值。综述胡桃属植物分子系统发育和生物地理方面的研究历史和进展,对美国白核桃及我国特有种麻核桃、野核桃和胡桃楸的归属及系统地位等有争议的问题进行分析,对该属植物遗传多样性、起源分布与进化历史方面的研究前景进行展望。随着分子生物技术的迅速发展,预计二代测序技术将成为研究非模式植物胡桃属系统发育和生物地理的有利工具。 | 赵鹏 周惠娟 刘占林 Keith E. Woeste 呼东峰 党萌 李忠虎 王玛丽 赵桂仿 | 2014 | 林业科学2014，50，11： | 5 |
| 4 | Effects of gibberellin A_(4/7), 6-benzylaminopurine and chlormequat chloride on the number of male and female strobili and immature cones in Chinese Pine (Pinus tabuliformis) with foliar sprays显示文摘Three kinds of plant growth regulators, gibberellinA 4/7 (GA 4/7 ), 6-benzylaminopurine (BA), and chlormequat chloride (CCC), were evaluated for their ability to promote strobilus and cone production in a Chinese pine (Pinus tabuliformis Carr.) clonal seed orchard. Treatments (0, 250, 500, or 1000 mg L -1 ) were applied during three periods (June- July, July-August and August-September) in 2005. Of the three plant growth regulators, GA 4/7 was the best for promoting flower and cone production. Trees sprayed with GA 4/7 (500 mg L -1 ) from June to September had significantly more female strobili and immature cones than controls and other treatments (p≤0.0001). The best time to apply GA 4/7 was in June. BA at 500 mg L -1 significantly increased female flower and immature cone production, compared to other treatments and controls (p≤0.0001), while promotion of male strobili was unchanged. Chlormequat chloride at 1000 mg L -1 significantly increased the number of male strobili, compared to the other treatments (p≤0.0001). Spraying CCC at500 mg L -1 significantly increased the number of female strobili, compared to controls (p<0.05), but it had no effect on immature cone production (p>0.05). These results are important for improving seed production and seed orchard management of Chinese pine. | ZHAO Peng Keith E. Woeste ZHANG Shuo-xin YANG Peihua FAN Jun-feng HUANG Zhong-lian MA Zhen-hua | 2011 | Journal of Forestry Research2011，22，3： | 3 |
| 5 | Gene flow among wild and cultivated common walnut(Juglans regia)trees in the Qinling Mountains revealed by microsatellite markers显示文摘Patterns of gene flow and gene introgres sion can be used to assess the risk of genetic pollution of wild forest trees from widespread cultivated trees.A comprehensive understanding of the genetic relationships and levels of gene flow among wild and cultivated common walnut(Juglans regia)has become an urgent issue.Using twelve microsatellite markers,we investigated the genetic diversity and gene flow between cultivated and wild trees of J.regia in the Qinling Mountains,China.A high level of genetic variation was detected in both cultivated and wild trees.The mean number of alleles per locus was 17.5.Observed heterozygosity(HO)and expected heterozygosity(HE)were 0.777 and 0.800,respectively.Pollination of mother trees was not by nearest neighbors,and a paternity of 60.7%of offspring evaluated could not be assigned to a local,sampled tree.Pollen flow from cultivated trees to wild trees was infrequent(5.4%),and selfing rates ranged from zero to 25.0%.Male parents were located from 0 to 1005 m from their female partners,with an average pollination distance of 285.1 m.These results are discussed in light of the cultivated species’diversity,outlining the frequent spontaneous genetic contributions from the wild to the cultivated compartment.In addition,the pollen flow parameters provide useful information about the dynamics of pollen movement within J.regia populations. | Huijuan Zhou Peng Zhao Keith Woeste Shuoxin Zhang | 2021 | Journal of Forestry Research2021，32，5： | 0 |
| 6 | Pan-genome and transcriptome analyses provide insights into genomic variation and differential gene expression profiles related to disease resistance and fatty acid biosynthesis in eastern black walnut (Juglans nigra)显示文摘Walnut(Juglans)species are used as nut crops worldwide.Eastern black walnut(EBW,Juglans nigra),a diploid,horticultural important woody species is native to much of eastern North America.Although it is highly valued for its wood and nut,there are few resources for understanding EBW genetics.Here,we present a high-quality genome assembly of J.nigra based on Illumina,Pacbio,and HiC technologies.The genome size was 540.8 Mb,with a scaffold N50 size of 35.1 Mb,and 99.0%of the assembly was anchored to 16 chromosomes.Using this genome as a reference,the resequencing of 74 accessions revealed the effective population size of J.nigra declined during the glacial maximum.A single whole-genome duplication event was identified in the J.nigra genome.Large syntenic blocks among J.nigra,Juglans regia,and Juglans microcarpa predominated,but inversions of more than 600 kb were identified.By comparing the EBW genome with those of J.regia and J.microcarpa,we detected InDel sizes of 34.9 Mb in J.regia and 18.3 Mb in J.microcarpa,respectively.Transcriptomic analysis of differentially expressed genes identified five presumed NBS-LRR(NUCLEOTIDE BINDING SITELEUCINE-RICH REPEAT)genes were upregulated during the development of walnut husks and shells compared to developing embryos.We also identified candidate genes with essential roles in seed oil synthesis,including FAD(FATTY ACID DESATURASE)and OLE(OLEOSIN).Our work advances the understanding of fatty acid bioaccumulation and disease resistance in nut crops,and also provides an essential resource for conducting genomics-enabled breeding in walnut. | Huijuan Zhou Feng Yan Fan Hao Hang Ye Ming Yue Keith Woeste Peng Zhao Shuoxin Zhang | 2023 | Horticulture Research2023，10，3： | 0 |
| 7 | Measuring tree stem diameters and straightness with depth-image computer vision显示文摘Current techniques of forest inventory rely on manual measurements and are slow and labor intensive.Recent developments in computer vision and depth sensing can produce accurate measurement data at significantly reduced time and labor costs.We developed the ForSense system to measure the diameters of trees at various points along the stem as well as stem straightness.Time use,mean absolute error(MAE),and root mean squared error(RMSE)metrics were used to compare the system against manual methods,and to compare the system against itself(reproducibility).Depth-derived diameter measurements of the stems at the heights of 0.3,1.4,and 2.7 m achieved RMSE of 1.7,1.5,and 2.7 cm,respectively.The ForSense system produced straightness measurement data that was highly correlated with straightness ratings by trained foresters.The ForSense system was also consistent,achieving sub-centimeter diameter difference with subsequent measures and less than 4%difference in straightness value between runs.This method of forest inventory,which is based on depth-image computer vision,is time efficient compared to manual methods and less computationally and technologically intensive compared to Structure-from-Motion(SFM)photogrammetry and ground-based LiDAR or terrestrial laser scanning(TLS). | Hoang Tran Keith Woeste Bowen Li Akshat Verma Guofan Shao | 2023 | Journal of Forestry Research2023，34，5： | 0 |
| 8 | 美国农作物育种与农村发展显示文摘农作物育种家为美国农业和经济发展做出了巨大贡献。他们通过提高农业生产效益,改善了农民的经济条件,推动了农村社区的结构调整和发展。自第二次世界大战以来,通过鼓励农业产业化生产模式和补贴政策有力地促进了农业发展和农村生活水平的提高。育种家为农业开发新技术,提供适宜的品种,其主要贡献在于培育适应环境变化和机械化收割、运输的高产作物新品种。随着社会、技术和经济的不断变化,农业生产经营模式不断演化,农村人口不断向外转移。与此同时,多种社会、经济因素促使本土膳食主义、社区农业等新型食品系统模式产生。可持续发展指数反映了大型零售商希望消费者与食品生产之间关系更透明的愿望。这些因素,连同美国对农村生活质量的关注,为育种家解决新的客户需要、开发符合其价值观的技术以及以新的方式服务传统客户提供了机遇。 | 何艳琴 Keith E Woeste Sterling B Blanche Karen A Moldenhaver C Dana Nelson | 2012 | 中国种业2012，，8： | 0 |
| 9 | The de novo genome assembly of Tapiscia sinensis and the transcriptomic and developmental bases of androdioecy显示文摘Tapiscia sinensis(Tapisciaceae)possesses an unusual androdioecious breeding system that has attracted considerable interest from evolutionary biologists.Key aspects of T.sinensis biology,including its biogeography,genomics,and sexlinked genes,are unknown.Here,we report the first de novo assembly of the genome of T.sinensis.The genome size was 410 Mb,with 22,251 predicted genes.Based on whole-genome resequencing of 55 trees from 10 locations,an analysis of population genetic structure indicated that T.sinensis has fragmented into five lineages,with low intrapopulation genetic diversity and little gene flow among populations.By comparing whole-genome scans of male versus hermaphroditic pools,we identified 303 candidate sex-linked genes,79 of which(25.9%)were located on scaffold 25.A 24-kb region was absent in hermaphroditic individuals,and five genes in that region,TsF-box4,TsF-box10,TsF-box13,TsSUT1,and TsSUT4,showed expression differences between mature male and hermaphroditic flowers.The results of this study shed light on the breeding system evolution and conservation genetics of the Tapisciaceae. | Peng Zhao Guiliang Xin Feng Yan Huan Wang Xiaolong Ren Keith Woeste Wenzhe Liu | 2020 | Horticulture Research2020，7，1： | 0 |
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