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| 1 | The linkage maps of Dendrobium species based on RAPD and SRAP markers显示文摘Dendrobium plants are used commonly as tonic herbs and health food in many Asian countries,especially in China.Here we report the genetic map construction of two Dendrobium species with a double pseudo-testcross strategy using random amplified polymorphic DNA(RAPD) and sequence-related amplified polymorphism(SRAP) markers.A F1 mapping population of 90 individuals was developed from a cross between D.officinale and D.hercoglossum.A total of 307 markers,including 209 RAPD and 98 SRAP,were identified and used for genetic linkage group(LG) analysis.The D.officinale linkage map consisted of 11 major linkage groups and 3 doublets,which covered 629.4 cM by a total of 62 markers with an average locus distance of 11.2 cM between two adjacent markers.The D.hercoglossum linkage map contained 112 markers mapped on 15 major and 4 minor linkage groups,spanning a total length of 1,304.6 cM with an average distance of 11.6 cM between two adjacent markers.The maps constructed in this study covered 92.7% and 82.7% of the D.hercoglossum and D.officinale genomes respectively,providing an important basis for the mapping of horticultural and medicinal traits and for the application of marker-assisted selection in Dendrobium breeding program. | Dawei Xue Shangguo Feng Hongyan Zhao Hua Jiang Bo Shen Nongnong Shi Jiangjie Lu Junjun Liu Huizhong Wang | 2010 | Journal of Genetics and Genomics2010，37，3： | 26 |
| 2 | Synthesis and supercapacitor characteristics of PANI/CNTs composites显示文摘The polyaniline coated multi-walled carbon nanotubes (PANI/CNTs) composites were synthesized by in situ polymerization. The microstructure and component of the composites were characterized by scanning electron microscope (SEM), Fourier transfer infrared spectroscopy (FTIR), X-ray diffraction (XRD), and N2 adsorption BET. The electrochemical performances of the samples were tested by cyclic voltammogram (CV), electrochemical impedance spectroscopy (EIS) and galvanostatic charge/discharge. The results showed that the thickness of PANI layer on the surface of CNTs was ca. 50 nm. The specific capacitance of the ob- tained composite material was about 305.3 F g-1, which was much better than the specific capacitance of the pure CNTs (31 F g-1). | LI Fang SHI JunJun QIN Xue | 2010 | Chinese Science Bulletin2010，55，11： | 6 |
| 3 | Up-regulation of glycolipid transfer protein by bicyclol causes spontaneous restriction of hepatitis C virus replication显示文摘Bicyclol is a synthetic drug for hepatoprotection in clinic since 2004. Preliminary clinical observations suggest that bicyclol might be active against hepatitis C virus(HCV) with unknown mechanism. Here, we showed that bicyclol significantly inhibited HCV replication in vitro and in hepatitis C patients. Using bicyclol as a probe, we identified glycolipid transfer protein(GLTP) to be a novel restrictive factor for HCV replication. The GLTP preferentially bound host vesicle-associated membrane protein-associated protein-A(VAP-A) in competition with the HCV NS5 A, causing an interruption of the complex formation between VAP-A and HCV NS5 A. As the formation of VAP-A/NS5 A complex is essential for viral RNA replication, up-regulation of GLTP by bicyclol reduced the level of VAP-A/NS5 A complex and thus inhibited HCV replication. Bicyclol also exhibited an inhibition on HCV variants resistant to direct-acting antiviral agents(DAAs) with an efficacy identical to that on wild type HCV. In combination with bicyclol, DAAs inhibited HCV replication in a synergistic fashion. GLTP appears to be a newly discovered host restrictive factor for HCV replication, Up-regulation of GLTP causes spontaneous restriction of HCV replication. | Meng-Hao Huang Hu Li Rong Xue Jianrui Li Lihua Wang Junjun Cheng Zhouyi Wu Wenjing Li Jinhua Chen Xiaoqin Lv Qiang Li Pei Lan Limin Zhao Yongfeng Yang Zonggen Peng Jiandong Jiang | 2019 | Acta Pharmaceutica Sinica B2019，9，4： | 3 |
| 4 | Numerical study of water mist centerline velocity in microgravity显示文摘With water mist regarded as a promising fireextinguishing agent in spacecraft,it is necessary to investigate characteristics of water mist in microgravity.In this paper,FDS 6.0 is used to measure the centerline velocity of water mist with various initial velocities and the spray distance.The droplets will approach their terminal velocity after leaving the nozzle in normal-gravity,which agrees with the theoretical calculation.The velocity of water mist declines in microgravity.The polynomials of the velocity change are given in microgravity,which makes it possible to obtain the velocity with the spray distance increase when arbitrary initial velocity is given. | Xue Han Junjun Tao Jun Qin | 2014 | Chinese Science Bulletin2014，59，25： | 2 |
| 5 | Physiological,Biochemical and Molecular Responses of Barley Seedlings to Aluminum Stress显示文摘Barley(Hordeum vulgare L.)is one of the most Aluminum(Al)sensitive cereal species.In this study,the physiological,biochemical,and molecular response of barley seedlings to Al treatment was examined to gain insight into Al response and tolerance mechanisms.The results showed that superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activity were inhibited to different degrees following Al exposure.The MDA content also significantly increased with increasing Al concentrations.SRAP results indicated significant differences between Al treatments and controls in terms of SRAP profile,and the genomic template stability(GTS)decreased with increasing Al concentration and duration.These integrative results help to elucidate the underlying mechanisms that the barley response to Al toxicity. | Xiaoqin Zhang Tao Tong Bin Tian Yunxia Fang Jiangjie Pan Junjun Zheng Dawei Xue | 2019 | Phyton-International Journal of Experimental Botany2019，88，3： | 2 |
| 6 | Establishment of two-dimensional gel electrophoresis for soybean protein isolate and its application显示文摘To optimize the conditions for the establishment of two-dimensional gel electrophoresis(2-DE)of soybean protein isolate(SPI),we investigated Ampholine mixture,anodic and cathodic electrolytes,loading amount of sample,acrylamide concentration,p H gradient and gel staining method in twodimensional gel electrophoresis to optimize the protein imaging conditions in two-dimensional gel.The results of mixed-level design experiments showed that Ampholine,loading amount and gel staining method had significant effect(P<0.05)on 2-DE of SPI.The optimal conditions were Ampholine mixture(pH 3–10+pH 5–7 or pH 4–6+pH 5–7),loading amount of 2 mg sample and silver staining.Although the acrylamide concentration of the gel,the p H gradient,the anodic and cathodic electrolyte solutions had significant statistical effects on the protein separation degree,the complexity of the protein composition and the visibility of the gel images were more inclined to the 12%gel,the 3–10 pH gradient and the H3PO4/NaOH electrolyte.According to the established conditions,the hydrolyzed products of SPI emulsion were determined by 2-DE,and the dynamic changes of protein in the process of enzymatic hydrolysis were described. | Xinkai Lu Yaoming Cui Junjun Guan Xue Liu Hao Zhu Xuyang Ji Jianzhang Zheng Yunlong Cheng Xiaofei Fu | 2020 | Grain & Oil Science and Technology2020，3，3： | 2 |
| 7 | ALYREF mediates RNA m^(5)C modification to promote hepatocellular carcinoma progression显示文摘Dear Editor,The incidence of hepatocellular carcinoma(HCC)is increasing in many countries due to hepatic virus infection and unhealthy diet habits,and the unsatisfactory overall survival rate calls for novel treatments with higher efficacy.1 Recent studies have shown that RNA modifications are likely to participate in the progression of HCC,2 suggesting new methods for tumor growth control.Specifically,5-methylcytosine(m5C)is one of the most interesting RNA modification types,as accumulating evidence suggests its role in the promotion of HCC tumorigenesis.3 The RNA methyltransferase Aly/REF export factor(ALYREF)is considered one type of“reader”protein located in the nucleus that recognizes and binds directly with m5C sites in RNA and facilitates the export of RNA from the nucleus to the cytoplasm.4 Notably,ALYREF is considered a promising target for diagnosis and prognosis prediction.5 However,until now,the low number of related studies has limited the understanding of the mechanism of the HCC-promoting effects of ALYREF. | Chen Xue Xinyu Gu Qiuxian Zheng Qingmiao Shi Xin Yuan Yuanshuai Su Junjun Jia Jianwen Jiang Juan Lu Lanjuan Li | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 2 |
| 8 | Numerical and experimental study on the diffusion property of difluoromethane (HFC-32) in leakage 显示文摘 | Li Yunong Tao Junjun Han Yuchong Han Xue Qin Jun | 2014 | Procedia Engineering2014，，71： | 1 |
| 9 | Nitrous oxide emissions from three temperate forest types in the Qinling Mountains,China显示文摘To understand soil N2O fluxes from temperate forests in a climate-sensitive transitional zone,N2O emissions from three temperate forest types(Pinus tabulaeformis,PTT;Pinus armandii,PAT;and Quercus aliena var.acuteserrata,QAT)were monitored using the static closed-chamber method from June 2013 to May 2015 in the Huoditang Forest region of the Qinling Mountains,China.The results showed that these three forest types acted as N2O sources,releasing a mean combined level of 1.35±0.56 kg N2O ha^-1 a^-1,ranging from0.98±0.37 kg N2O ha^-1 a^-1 in PAT to 1.67±0.41 kg N2O ha^-1 a^-1 in QAT.N2O emission fluctuated seasonally,with highest levels during the summer for all three forest types.N2O flux had a significantly positive correlation with soil temperature at a depth of 5 cm or in the water-filled pore space,where the correlation was stronger for temperature than for the water-filled pore space.N2O flux was positively correlated with available soil nitrogen in QAT and PAT.Our results indicate that N2O flux is mainly controlled by soil temperature in the temperate forest in the Qinling Mountains. | Wei Xue Changhui Peng Huai Chen Hui Wang Qiuan Zhu Yanzheng Yang Junjun Zhang Wanqin Yang | 2019 | Journal of Forestry Research2019，30，4： | 0 |
| 10 | Low-threshold lasing in a plasmonic laser using nanoplate InGaN/GaN显示文摘Plasmonic nanolaser as a new type of ultra-small laser,has gain wide interests due to its breaking diffraction limit of light and fast carrier dynamics characters.Normally,the main problem that need to be solved for plasmonic nanolaser is high loss induced by optical and ohmic losses,which leads to the low quality factor.In this work,InGaN/GaN nanoplate plasmonic nanolaser with large interface area were designed and fabricated,where the overlap between SPs and excitons can be enhanced.The lasing threshold is calculated to be~6.36 kW/cm^(2),where the full width at half maximum(FWHM)drops from 27 to 4 nm.And the fast decay time at 502 nm(sharp peak of stimulated lasing)is estimated to be 0.42 ns.Enhanced lasing characters are mainly attributed to the strong confinement of electromagnetic wave in the low refractive index material,which improve the near field coupling between SPs and excitons.Such plasmonic laser should be useful in data storage applications,biological application,light communication,especially for optoelectronic devices integrated into a system on a chip. | Ting Zhi Tao Tao Xiaoyan Liu Junjun Xue Jin Wang Zhikuo Tao Yi Li Zili Xie Bin Liu | 2021 | Journal of Semiconductors2021，42，12： | 0 |
| 11 | Effects of paraprobiotics on bile acid metabolism and liver health in largemouth bass(Micropterus salmoides)fed a cottonseed protein concentrate-based diet显示文摘Cottonseed protein concentrate is a sustainable fishmeal alternative in aquafeed.A 10-week experiment was conducted to investigate the effects of a cottonseed protein concentrate-based diet with and without multi-strain yeast fractions(MsYF)on growth,bile acid metabolism,and health in largemouth bass.Four hundred fish(54.0±0.0 g)were casually distributed into 16 tanks(4 replicates/diet).Fish were fed with 4 iso-nitrogen and iso-energetic diets 3 times daily,including a fishmeal diet(FM),a soy protein concentrate-based diet(SPC;replacing 81%fishmeal protein),a cottonseed protein concentrate-based diet(CPC;replacing 81%fishmeal protein),and a CPC diet supplemented with 800 mg/kg MsYF(CPCY).Results showed that the survival of SPC was the lowest,i.e.,48%,with no apparent diet effect among other treatments;we omitted the SPC in additional analyses.Fish fed cottonseed protein concentrate-based diets showed lower growth than FM(P<0.05).Fish fed CPC showed the highest nuclear dense hepatic phenotypes ratio(50%),followed by CPCY(33%)and FM(17%).Further,dietary CPC increased hepatic total cholesterol and triglyceride levels with concurrently increased cholesterol syn-thesis but decreased triglyceride synthesis-associated transcription levels(P<0.05).Furthermore,di-etary CPC increased bile acid synthesis but decreased bile acid transport-associated transcription levels(P<0.05),and then induced an increment of plasma cholic acid and hepatic chenodeoxycholic acid content and the decrement of genus Romboustia(P<0.05).Regarding the effect of MsYF,fish fed CPCY reduced hepatic lipid accumulation and total plasma bile acid content(P<0.05)compared to CPC,suggesting an improvement in liver health.Also,dietary MsYF could reverse the microbiota community structure showing a similar gut microbial composition to FM.In conclusion,81%of fishmeal protein replaced by cottonseed protein concentrate suppressed growth and liver health,while dietary MsYF might mitigate the negative impact of a high cottonseed protein concentrate level diet on liver functions via gut microbiota regulation. | Xiaoze Xie Xiaofang Liang Hao Wang Qiang Zhu Junjun Wang Ying Chang Eric Leclercq Min Xue Jie Wang | 2023 | Animal Nutrition2023，，2： | 0 |
| 12 | Intratumoral bacteria interact with metabolites and genetic alterations in hepatocellular carcinoma显示文摘Dear Editor,Hepatocellular carcinoma(HCC),which ranks globally as the third leading cause of cancer-related deaths,is highly prevalent,and most patients are diagnosed with advanced-stage cancer when treatments are largely ineffective.1 Thus,there is an urgent need for earlier diagnosis to improve HCC patient outcomes.Although many types of tumors have intratumor bacteria,the tumor microbiome is poorly characterized because of limitations in the technology for detection.Recently,Nejman et al. | Chen Xue Junjun Jia Xinyu Gu Lin Zhou Juan Lu Qiuxian Zheng Yuanshuai Su Shusen Zheng Lanjuan Li | 2022 | Signal Transduction and Targeted Therapy2022，7，10： | 0 |
| 13 | Weldable and closed-loop recyclable monolithic dynamic covalent polymer aerogels显示文摘Owing to their low density,high porosity and unique micro-nanostructures,aerogels are attractive for application in various fields;however,they suffer from shrinkage and/or cracking during preparation,mechanical brittleness,low production efficiency and non-degradation.Herein,we introduce the concept of dynamic covalent polymer chemistry to produce a new class of aerogels—referred to as DCPAs.The resulting lightweight DCPAs have the potential to be prepared on a large scale and feature high porosity(90.7%–91.3%),large degrees of compression(80%strain)and bending(diametral deflection of 30 mm)without any cracks,as well as considerable tensile properties(an elongation with a break at 32.7%).In addition,the DCPAs showcase the emergent characteristics of weldability,repairability,degradability and closed-loop recyclability that are highly desirable for providing versatile material platforms,though hardly achieved by traditional aerogels.Taking advantage of their robust porous structures,we demonstrate the potential of DCPAs for applications in thermal insulation and emulsion separation.These findings reveal that the dynamic covalent bond strategy would be generalized for the production of a new generation of aerogels with customized features for functioning in the field of intelligent and sustainable materials. | Xinhai Zhang Jun Zhao Kai Liu Guangfeng Li Dong Zhao Zhaoming Zhang Junjun Wan Xue Yang Ruixue Bai Yongming Wang Wei Zhang Xuzhou Yan | 2022 | National Science Review2022，9，9： | 0 |
| 14 | Genome-Wide Identification of the F-box Gene Family and Expression Analysis under Drought and Salt Stress in Barley显示文摘The F-box protein-encoding gene family plays an essential role in plant stress resistance.In present study,126 non-redundant F-box genes were identified in barley(Hordeum vulgare L.,Hv).The corresponding proteins contained 165–887 amino acid residues and all were amphiphilic,except 5 proteins.Phylogenetic analysis of F-box protein sequences in barley and stress-related F-box protein sequences in wheat and Arabidopsis thaliana(At)was used to classify barley F-box genes are divided into 9 subfamilies(A–I).A structure-based sequence alignment demonstrated that F-box proteins were highly conserved with a total of 10 conserved motifs.In total,124 F-box genes were unevenly distributed on 7 chromosomes;another 2 genes have not been anchored yet.The gene structure analysis revealed high variability in the number of exons and introns in F-box genes.Comprehensive analysis of expression profiles and phylogenetic tree analysis,a total of 12 F-box genes that may be related to stress tolerance in barley were screened.Of the 12 detected F-box genes,8 and 10 were upregulated after drought and salt stress treatments,respectively,using quantitative real-time polymerase chain reaction(qRT-PCR).This study is the first systematic analysis conducted on the F-box gene family in barley,which is of great importance for clarifying this family’s bioinformatic characteristics and elucidating its function in barley stress resistance.These results will serve as a theoretical reference for subsequent research on molecular regulation mechanisms,genetic breeding,and improvement. | Lantian Zhang Siyi Wang Yuyu Chen Mengyuan Dong Yunxia Fang Xian Zhang Tao Tong Ziling Zhang Junjun Zheng Dawei Xue Xiaoqin Zhang | 2020 | Phyton-International Journal of Experimental Botany2020，89，2： | 0 |
| 15 | Analysis of a landfill cover without geomembrane using varied particle sizes of recycled concrete显示文摘Previous studies have demonstrated the effectiveness of a novel three-layer landfill cover system constructed with recycled concrete aggregates(RCAs)without geomembrane in both laboratory and field.However,no systematic investigation has been carried out to optimize the combination of the particle sizes for fine-grained RCAs(FRC)and coarse-grained RCAs(CRC)that can be used for the three-layer landfill cover system.The aim of this paper is to assist engineers in designing the three-layer landfill cover system under a rainfall of 100-year return period in humid climate conditions using an easily controlled soil parameter D10 of RCAs.The numerical study reveals that when D10 of FRC increases from 0.05 mm to 0.16 mm,its saturated permeability increases by 10 times.As a result,a larger amount of rainwater infiltrates into the cover system,causing a higher lateral diversion in both the top FRC and middle CRC layers.No further changes in the lateral diversion are observed when the D10 value of FRC is larger than 0.16 mm.Both the particle sizes of FRC and CRC layers are shown to have a minor influence on the percolation under the extreme rainfall event.This implies that the selection of particle sizes for the FRC and CRC layers can be based on the availability of materials.Although it is well known that the bottom layer of the cover system should be constructed with very fine-grained soils if possible,this study provides an upper limit to the particle size that can be used in the bottom layer(D10 not larger than 0.02 mm).With this limit,the three-layer system can still minimize the water percolation to meet the design criterion(30 mm/yr)even under a 100-year return period of rainfall in humid climates. | Charles Wang Wai Ng Cheuk Lam Ng Junjun Ni Haowen Guo Qi Zhang Qiang Xue Rui Chen | 2023 | Journal of Rock Mechanics and Geotechnical Engineering2023，15，5： | 0 |
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