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| 1 | Methylation profile of the promoter CpG islands of 31 genes that may contribute to colorectal carcinogenesis显示文摘AIM: To establish the methylation profile of the promoter CpG islands of 31 genes that might play etiological roles in colon carcinogenesis.METHODS: The methylation specific PCR in conjunction of sequendng verification was used to establish the methylationprofile of the promoter CpG islands of 31 genes in colorectal cancer (n = 65), the neighboring non-cancerous tissues (n = 5), colorectal adenoma (n = 8), and normal mucosa (n = 1). Immunohistochemically, expression of 10 genes was assessed on the home-made tissue microarrays of tissues from 58 patients. The correlation of tumor specific changes with each of clinical-pathologic features was scrutinized with relevant statistic tools.RESULTS: In comparison with the normal mucosa of the non-cancer patients, the following 14 genes displayed no tumor associated changes: breast cancer 1, early onset (BRCA1), cadherin 1, type 1, E-cadherin (epithelial) (CDH1),death-associated protein kinase 1 (DAPK1), DNA (cytosine-5-)-methyltransferase 1 (DNMT1), melanoma antigen, family A, 1 (directs expression of antigen MZ2-E) (MAGEA1), tumor suppressor candidate 3 (N33), cyclin-dependent kinase inhibitor 1A (p21, Cipl) (p21^WAF1), cyclin-dependent kinase inhibitor 1B (p27, 10pl) (p27^WAF1), phosphatase and tensin hornolog (mutated in multiple advanced cancers 1) (PTEN), retinoic acid receptor, beta (RAR-, Ras association (RaIGDS/AF-6) domain family 1 C (RASSFIC), secreted frizzled-related protein 1 (SFRP1), tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinfiammatory) (TIMP3),and von HippeI-Lindau syndrome (VHL). The rest 17 targets exhibited to various extents the tumor associated changes.As changes in methylation of the following genes occurred marginally, their impact on the formation of colorectal cancer were trivial: adenomatous polyposis coli (APC) (8%, 5165),Ras association (RaIGDS/AF-6) domain family 1A (RASSFIA) (3%, 2/65) and cyclin-dependent kinase inhibitor 2A,alternated reading frame Co14~) (6%, 4/65). The following genes exhibited moderate changes in rnethylation: O-6rnethylguanine-DNA rnethyltransferase (MGMT) (20%, 13/65),rnutL hornolog 1, colon cancer, nonpolyposis type 2 (E. coli) (hMLH1) (18%, 12/65), cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) P16^NK4a) (10%, 10/65),rnethylated in tumor 1 (MINT1) (15%, 10/65), methylated in tumor 31 (MINT31) (11%, 7/65). The rest changed greatly in the rnethylation pattern in colorectal cancer (CRC): cyclin A1 (cyclin al) (100%, 65/65), caudal type homeobox transcriptdon factor 1 (CDX1) (100%, 65/65), RAR(85%, 55/65), myogenic factor 3 (MYOD1) (69%, 45/65),cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)(p15^INK4b) (68%, 44/65), prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (COX2) (72%, 47/65), cadherin 13, H-cadherin (heart) (CDH13) (65%, 42/65), CAAX box 1 (OO~/) (58%, 38/65),tumor protein p73 (p73) (63%, 41/65) and Wilrns tumor 1 (WT/) (58%, 38/65). However, no significant correlation of changes in rnethylation with any given clinical-pathological features was detected. Furthermore, the frequent changes in rnethylation appeared to be an early phase event of colon carcinogenesis. The in situ expression of 10 genes was assessed by the irnrnunohistochernical approach at the protein level: CDH1, CDH13, COX2, cyclin A1, hMLH1,MGMT, p14^ARF, p73, RAR-, and TIMP3 genes in the context of the rnethylation status in colorectal cancer. No clear correlation between the hyperrnethylation of the promoter CpG islands and the negative expression of the genes was established.CONCLUSION: The methylation profile of 31 genes was established in patients with colon cancer and colorectal adenornas, which provides new insights into the DNA rnethylation mediated mechanisms underlying the carcinogenesis of colorectal cancer and may be of prognostic values for colorectal cancer. | Xiao-UXu JianYu Hong-YuZhang Meng-HongSun JunGu XiangDu Da-RenShi PengWang Zhen-HuaYang Jing-DeZhu | 2004 | World Journal of Gastroenterology2004，10，23： | 43 |
| 2 | Survivin antisense compound inhibits proliferation and promotes apoptosis in liver cancer cells显示文摘AIM: To evaluate the effects of survivin on cell proliferation and apoptosis in liver cancer.METHODS: MTT assay was used to generate and optimize phosphorothioate antisense oligonucleotides (ODNs)LipofectamineTM2000 (LiP) compound by varying ODNs (μg):LiP (μL) ratios from 1:0.5 to 1:5. Then, liver cancer cells (HepG2) were transfected with the compound. By using RT-PCR and Western blot, the expression levels of survivin mRNA and proteins were detected in HepG2 cells treated with antisense compounds (ODNs:LiP = 1:4), and compared with those treated with sense compounds (1:4) as control.MTT assay was applied to the determination of cell proliferation in HepG2 cells. Active caspase-3 was evaluated by flow cytometric analysis. The morphological changes were assessed by electron microscopy. Laser scanning confocal microscopy was performed to detect the subcellular localization of survivin proteins in treated and untreated cells.RESULTS: Antisense compounds (1:4) down-regulated survivin expression (mRNA and protein) in a dose-dependent manner with an IC50 of 250 nmol/L. Its maximum effect was achieved at a concentration of 500 nmol/L, at whichmRNA and protein levels were down-regulated by 80%.The similar results were found in MTT assay. Antisense compound (1:4)-treated cells revealed increased caspase3-like protease activity compared with untreated cells.Untreated cells as control were primarily negative for the presence of active-caspase-3. As shown by transmission electron microscopy, treated cells with antisense compounds (1:4) resulted in morphological changes such as blebbing and loss of microvilli, vacuolization in the cytoplasm,condensation of the cytoplasm and nuclei, and fragmented chromatin. Immunofluorescence analysis confirmed the presence of survivin protein pool inside the cytoplasm in untreated cells. Labeled-FITC immunofluorescence staining of survivin clearly showed that survivin was distributed mainly in a spotted form inside the cytoplasm. Whereas cells treated with antisense compounds were rare and weak inside the cytoplasm.CONCLUSION: Down-regulation of survivin expression induced by the antisense compounds reduces tumor growth potential, promotes apoptosis and affects the localization of survivin proteins in HepG2 cells. Furthermore, survivin protein is a key molecule associated with proliferation and apoptosis, and antisense oligonucleotides targeting survivin have a bright prospect in the therapy of liver cancer. | De-JianDai Cai-DeLu Ri-YongLai Jun-MingGuo HuaMeng Wei-ShengChen JunGu | 2005 | World Journal of Gastroenterology2005，11，2： | 30 |
| 3 | Methylation profile of the promoter CpG islands of 14 “drug-resistance”genes in hepatocellular carcinoma显示文摘AIM: To establish the DNA methylation patterns of the promoter CpG islands of 14 'drug-resistance' genes in hepatocellular carcinoma (HCC).METHODS: The methylation specific polymerase chain reaction in conjunction with sequencing verification was used to establish the methylation patterns of the 14 genes in the liver tissues of four healthy liver donors, as well as tumor and the paired non-cancerous tissues of 30 HCC patients.RESULTS: While 11 genes (ATP-binding cassette, sub-family G (WHITE), member 2(ABCG2), activating transcription factor (ATF2), beta-2-microglobulin (B2M), deoxycytidine kinase(DCK, occludin (OCLN, v-raf-1 murine leukemia viral oncogene homolog (RAF/), ralA binding protein 1 (RALBP1),splicing factor (45 kD) (SPF45), S-phase kinase-associated protein 2 (p45) (SKP2), tumor protein p53 (Li-Fraumeni syndrome) (TP53) and topoisomerase (DNA) II beta (TOP2B) maintained the unmethylated patterns, three genes displayed to various extents the hypermethylation state in tumor tissues in comparison with the normal counterparts. The catalase (CAT) was hypermethylated in tumor and the neighboring non-cancerous tissue of one case (3.3%). Both glutathione S-transferase pi (GSTp/) (80%, 24/30 in tumor and 56.7%,17/30 in the paired non-cancerous tissues) and cystic fibrosis transmembrane conductance regulator, ATP-binding cassette (sub-family C, member 7) (CF-FR) (77%, 23/30 in tumor and 50%, 15/30 in the paired non-cancerous tissues) genes were prevalently hypermethylated in HCC as well as their neighboring non-cancerous tissues. No significant difference in the hypermethylation occurrence was observed between the HCC and its neighboring non-cancerous tissues.CONCLUSION: Hypermethylation of promoter CpG islands of both CF-FR and GSTpigenes occurs prevalently in HCC,which may correlate with the low expression of these two genes at the mRNA level and has the profound etiological and clinical implications. It is likely to be specific to the early phase of HCC carcinogenesis. | ShengDing Bang-DongGong JianYu JunGu Hong-YuZhang Zu-BinShang QiFei PengWang Jing-DeZhu | 2004 | World Journal of Gastroenterology2004，10，23： | 14 |
| 4 | New Meta-Heuristic for Combinatorial Optimization Problems:Intersection Based Scaling显示文摘Combinatorial optimization problems are found in many application fields such as computer science, engineering and economy. In this paper, a new efficient meta-heuristic, Intersection-Based Scaling (IBS for abbreviation),is proposed and it can be applied to the combinatorial optimization problems. The main idea of IBS is to scale the size of the instance based on the intersection of some local optima, and to simplify the search space by extracting the intersection from the instance, which makes the search more efficient. The combination of IBS with some local search heuristics of different combinatorial optimization problems such as Traveling Salesman Problem (TSP) and Graph Partitioning Problem (GPP) is studied, and comparisons are made with some of the best heuristic algorithms and meta-heuristic algorithms. It is found that it has significantly improved the performance of existing local search heuristics and significantly outperforms the known best algorithms. | PengZou ZhiZhou Ying-YuWan Guo-LiangChen JunGu | 2004 | Journal of Computer Science & Technology2004，19，6： | 5 |
| 5 | A novel protein-DNA interaction involved with the CpG dinucleotide at -30 upstream is linked to the DNA methylation mediated transcription silencing of the MAGE-A1 gene显示文摘To understand the DNA-methylation mediated gene silencing mechanisms, we analyzed in cell culture of the promoter function of the MAGE-A1 gene, which is frequently demethylated and over-expressed in human hepatocellular carcinoma. We have established the correlation of the DNA methylation of the promoter CpG island with expression status of this gene in a panel of the established liver cancer cell lines. The crucial CpG dinucleotide(s) within the minimal promoter subjected to the control mediated by DNA methylation with profound biological functions was also delineated.Furthermore, a novel sequence-specific DNA-protein interaction at the -30 CpG dinucleotide upstream of the gene was found having a vital part to play in the DNA methylation mediated transcription silencing of the MAGE-A1 gene. Our results would not only provide new insights into the DNA methylation mediated mechanisms over transcription of the MAGE-A1 gene, but also pave the way for further defining the cross-talk among DNA methylation, histone modification and chromatin remodeling in detail. | JieZHANG JianYU JunGU BaoMeiGAO YingJunZHAO PengWANG HongYuZHANG JingDeZHU | 2004 | Cell Research2004，14，4： | 3 |
| 6 | A New Deoxygenation Method for Taxanes Using Hypophosphorous Acid显示文摘An efficient and practical radical chain deoxygenation method by phosphorus centered radicals generated from hypophosphorous acid was developed in the synthesis of analogues of paclitaxel. | JunGU MengZHANG DaLiYIN JiYuGUO XiaoTianLIANG | 2004 | Chinese Chemical Letters2004，15，6： | 1 |
| 7 | Progressive gray mat ter atrophy in lacunar patients with vascular mild cognitive irn pairment显示文摘 | Grau-Olivares M Arboix A Jungue C | 2010 | Cerebrovasc Dis2010，30，2： | 1 |
| 8 | 2-methyl selenocysteine induces apoptosis through caspase activation in HL260 cells显示文摘 | Kim T JungU Cho DY | 2001 | Carcinogenesis2001，22，4： | 1 |
| 9 | Proximity effect among cellulose-degrading enzymesdisplayed on the Saccharomyces cerevisiae cell surface显示文摘 | JUNGU BAE KOUICHI KURODA MITSUYOSHIUEDA | 2015 | Applied and Environmental Microbiology2015，81，1： | 1 |
| 10 | Progressive gray matter atrophy in lacunar patients with vascular mild cognitive impairment显示文摘 | Grau-Olivares M Arboix A Jungue C | | 0，，02： | 1 |
| 11 | Anislanding detection method for a grid-connected systembased on the goertzel algorithm显示文摘 | Kim Jaehyung Kim Jungu Ji Younghyok | 2011 | IEEE Transactions onPower Electronics2011，26，4： | 1 |
| 12 | Progressive gray matter atrophy in lacunar patients with vascular mild cognitive impairment 显示文摘 | Grau-Olivares M Arboix A Jungue C | 2010 | Cerebrovasc Dis2010，30，2： | 1 |
| 13 | 求解组合优化问题的一种新的宏启发式算法:IBS显示文摘随着科学研究和工程技术的发展,许多科学研究领域和工程应用问题都涉及到了一些组合优化问题,而这些问题中存在着大量的NP难解(NP—hard)问题,所以NP难解问题的研究具有十分重要的理论意义和广泛的应用背景,而其研究成果对科学研究的发展以及国民经济的建设都起着极大的推动作用。 | PengZou ZhiZhou Ying-YuWan Guo-LiangChen JunGu | 2004 | Journal of Computer Science & Technology2004，19，C00： | 0 |
| 14 | 变形网格结构的快速评估算法显示文摘随着超大规模集成电路技术的进步,集成电路的复杂性在急剧增加。分级设计和IP(知识产权)重用技术变得极为重要。因此,面向宏模块布局的布图规划/布局技术在近10年来成为VLSI设计自动化领域的研究热点。即使简单的矩形放置问题(将n个矩形放置在一个平面的矩形区域内。 | SongChen Xian-LongHong She-QinDong Yu-ChunMa Chung-KuanCheng JunGu | 2004 | Journal of Computer Science & Technology2004，19，C00： | 0 |
| 15 | A buffer planning algorithm for chip-level floorplanning显示文摘This paper studies the buffer planning problem for interconnect-centric floorplanning for nanometer technologies. The dead-spaces are the spaces left unused within a placement that are not held by any circuit block. In this paper, we proposed a buffer planning algorithm based on dead space redistribution to make good use of dead-spaces for buffer insertion. Associated with circuit blocks under topological representations, the dead space can be redistributed by moving freely some circuit blocks within their rooms in the placement. The total area and the topology of the placement keep unchanged while doing the dead space redistribution. The number of nets satisfying the delay constraint can be increased by redistributing the dead space all over the placement, which has been demonstrated by the experimental results. The increment of the number of nets that meet delay constraint is 9% on an average. | CHENSong HONGXianlong DONGSheqin MAYuchun CAIYici Chung-KuanCheng JunGu | 2004 | Science in China(Series F)2004，47，6： | 0 |
| 16 | A ratiometric fluorescent sensor for tracking Cu(Ⅰ) fluctuation in endoplasmic reticulum显示文摘A two-photon ratiometric fluorescent sensor for Cu^+ in endoplasmic reticulum(ER), CNSB, was developed via coumarin/ASBD integration based on FRET mechanism. In solution, CNSB shows reversible, highly-specific ratiometric response to Cu^+ .Moreover, CNSB exhibits suitable K_d value, suggesting the possibility of detecting Cu^+ in the living cells. The probe can enter the MCF-7 cells easily and specifically locates in the ER. The highly specific ratiometric response of CNSB toward Cu^+ in MCF-7 cells provides the sensor the capacity to visualize both exogenous and endogenous Cu^+ in the ER via fluorescence imaging.Next, CNSB was utilized to detect the fluctuation and distribution of Cu^+ under ER stress in MCF-7 cells, which confirmed directly the relationship between Cu^+ enhancement and ER stress. Meanwhile, the two-photon ability of coumarin facilitated the sensor to visualize Cu^+ fluctuation via two-photon fluorescence imaging. In addition, the spatial distribution of Cu^+ in the heart slice of the 14-day-old rat was demonstrated using CNSB. This study demonstrates the promising potential of CNSB in clarifying the Cu^+ -dependent signaling in the ER stress-related diseases. | Jungu Guo Hao Yuan Yuncong Chen Zhongyan Chen Menglong Zhao Liang Zou Yi Liu Zhipeng Liu Qiang Zhao Zijian Guo Weijiang He | 2019 | Science China Chemistry2019，62，4： | 0 |
| 17 | Ultramicroscopic observation on the traumainduced neurodegeneration in the newborn rat brain显示文摘 | HuiyingZhang ZhengruiHe PingZhu JunGu WenchaoLiu LihuaCheng | 2004 | 中国组织化学与细胞化学杂志2004，13，3： | 0 |
| 18 | Fast Evaluation of Bounded Slice-Line Grid显示文摘Bounded Slice-line Grid (BSG). is an elegant representation of block placement, because it is very intuitionistic and has the advantage of handling various placement constraints. However, BSG has attracted little attention because its evaluation is very time-consuming. This paper proposes a simple algorithm independent of the BSG size to evaluate the BSG representation in O(nloglogn) time, where n is the number of blocks. In the algorithm, the BSG-rooms are assigned with integral coordinates firstly, and then a linear sorting algorithm is applied on the BSG-rooms where blocks are assigned to compute two block sequences, from which the block placement can be obtained in O(n log log n) time. As a consequence, the evaluation of the BSG is completed in O(n log log n) time, where n is the number of blocks. The proposed algorithm is much faster than the previous graph-based O(n2) algorithm. The experimental results demonstrate the efficiency of the algorithm. | SongChen Xian-LongHong She-QinDong Yu-ChunMa Chung-KuanCheng JunGu | 2004 | Journal of Computer Science & Technology2004，19，6： | 0 |
| 19 | Cue-induced resumption of heroin seeking in rats after abstinence: An alternative model of craving and relapse to drug abuse显示文摘AIM : We showed previously that long-term withdrawal from heroin self-administration results in persistence and resistance to extinction of the drug seeking elicited by environmental and discrete conditioned cues previously associated with heroin. The present study characterized the patterns of resumption of drug seeking induced by contextual, | WenhuaZhou FuqiangZhang ShuaienTang HuifenLiu JunGu GuodongYang | 2004 | 中国药理通讯2004，21，2： | 0 |
| 20 | Microwave-assisted Allylic Oxidation at C-13 Position of 14-Deoxysinenxan A显示文摘Microwave-assisted allylic oxidation at C-13 position of 14-deoxysinenxan A was described. This new method (150℃/10 min/5 bar on microwave synthesizer) led to a better yield of compound 1 and shorter reaction time. | JunGU MengZHANG DaLiYIN JiYuGUO XiaoTianLIANG | 2005 | Chinese Chemical Letters2005，16，4： | 0 |
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