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| 1 | Gelatin degradation assay reveals MMP-9 inhibitors and function of O-glycosylated domain显示文摘AIM: To establish a novel, sensitive and high-throughput gelatinolytic assay to define new inhibitors and compare domain deletion mutants of gelatinase B/matrix metalloproteinase (MMP)-9. METHODS: Fluorogenic Dye-quenched (DQ)TM-gelatin was used as a substrate and biochemical parameters (substrate and enzyme concentrations, DMSO solvent concentrations) were optimized to establish a highthroughput assay system. Various small-sized libraries (ChemDiv, InterBioScreen and ChemBridge) of hetero-cyclic, drug-like substances were tested and compared with prototypic inhibitors. RESULTS: First, we designed a test system with gelatin as a natural substrate. Second, the assay was validated by selecting a novel pyrimidine-2,4,6-trione (barbitu- rate) inhibitor. Third, and in line with present structural data on collagenolysis, it was found that deletion of the O-glycosylated region significantly decreased gelatinolytic activity (kcat/kM ± 40% less than full-length MMP-9). CONCLUSION: The DQTM-gelatin assay is useful in high-throughput drug screening and exosite targeting. We demonstrate that flexibility between the catalytic and hemopexin domain is functionally critical for gelatinolysis. | Jennifer Vandooren Nathalie Geurts Erik Martens Philippe E Van den Steen Steven De Jonghe Piet Herdewijn Ghislain Opdenakker | 2011 | World Journal of Biological Chemistry2011，2，1： | 3 |
| 2 | Does the stock market value bank diversification?显示文摘 | Lieven Baele Olivier De Jonghe Rudi Vander Vennet | 2007 | Journal of Banking and Finance2007，，7： | 2 |
| 3 | The effect of pH on heat denaturation and gel forming properties of soy proteins显示文摘 | Jacoba M.S Renkema Catriona M.M Lakemond Harmen H.J de Jongh Harry Gruppen Ton van Vliet | 2000 | Journal of Biotechnology2000，，3： | 2 |
| 4 | L’alimentation entérale précoce du traumatisé cranien显示文摘 | C De Deyne R De Jongh L Merckx J Deghislage R Heylen | 1998 | Annales francaises d’anesthesie et de reanimation1998，，2： | 2 |
| 5 | Complications of femoral and subclavian venous catheterization in critically ill patients:a randomized controlled trial显示文摘 | Merrer J De Jonghe B Golliot F | 2001 | JAMA2001，286，： | 1 |
| 6 | Thin Film Solid Oxide Fuel Cell with High Performance at Low Temperature显示文摘 | Visco S J Jonghe L C De | 1997 | Solid State Ionics1997，98，： | 1 |
| 7 | Factor stmcture and validity of the Dutch version of the Cohen-Mansfield agitation inventory (CMAI-D)显示文摘 | de Jonghe JFM Kat MG | 1996 | J Am Geriatr Soc1996，44，7： | 1 |
| 8 | Chemotherapy-induced pica and ano- rexia are reduced by common hepatic branch vagotomy in the rat 显示文摘 | De Jonghe B C Horn C C | 2008 | Am J Physiol Regul Integr Comp Physiol2008，294，3： | 1 |
| 9 | Processing and Properties of Particulate Composites from Coated Powders显示文摘 | Mitchell Tyrone D Jr De Jonghe Lutgard C | 1995 | J Am Ceram Soc1995，78，1： | 1 |
| 10 | Early enteral feeding in cranial trauma显示文摘 | De Deyne C De Jongh R Mercks L | 1998 | Ann Fr Anesth Reanim1998，17，2： | 1 |
| 11 | Combining psychotherapy and antide- pressants in the treatment of depression显示文摘 | Jonghe F Kool S Aalst' G | 2001 | Affect Disord2001，64，23： | 1 |
| 12 | Anesthesia and postoperative delirium in older adults undergoing hip surgery显示文摘 | Slor CJ de Jonghe JF Vreeswijk R | | 0，，07： | 1 |
| 13 | The physio- logical rationale of heat and moisture exchangers in post- laryngectomy pulmonary rehabilitation: a review显示文摘 | Zuur J K Muller S H de Jongh F H | 2006 | Eur Arch Otorhinolaryngol2006，263，1： | 1 |
| 14 | A test of Berggren' s model of dental fear and anxiety 显示文摘 | De Jongh A Schutjes M Aartman IHA | 2011 | Eur J Oral Sci2011，119，5： | 1 |
| 15 | Endogenous subclinical thyroid disorders, physical and cognitive function, depression, and mortality in older individuals显示文摘 | de Jongh RT Lips P van Schoor NM | 2011 | Eur J Endocrinol2011，165，4： | 1 |
| 16 | Survival and prognostic indicators in hepatitis B surface antigen-positive cirrhosis of the liv- er显示文摘 | Jongh F E Janssen H L de Man R A | 1992 | Gastroenterology1992，103，5： | 1 |
| 17 | Forensic data recovery from flash memory显示文摘 | Marcel Breeuwsma Martien de Jongh CoertKlaver etc | 2007 | Small Scale Device Forensics Journal2007，，： | 1 |
| 18 | Complications of femoral and subclavian venous catheterization in critically ill patients:a randomized controlled trial显示文摘 | Merrer J De Jonghe B Golliot F | 2001 | JAMA2001，286，6： | 1 |
| 19 | Treatment modalities in a dental fear clinic and the relation with general psychopathology and oral health variables 显示文摘 | Aartman IH de Jongh A | 1999 | Br Dent J1999，186，9： | 1 |
| 20 | Physical and chemical interactions in cold gelation of food proteins显示文摘 | ALTING A C DE JONGH H H J VISSCHERS R W | 2002 | J Agric Food Chem2002，50，16： | 1 |
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