|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 工程教育的环境显示文摘学习的环境(Context)是关于理解并学习知识和技能的一整套文化环境。我们认为,产品、过程或者系统的生命周期——构思-设计-实施-操作(以下简称CDIO)即为工程教育的环境,而非内容。教育的背景,我们传授的技能和我们所表达的态度,都表明构思-设计-实施-操作是一个工程师在社会中的职责。这是有效实践的第一准则所反映的中心思想,该准则就是CDIO准则1。本文首先回顾了职业工程实践的环境,指出了社会发展和技术更新带来的不变和变化着的各个方面。然后,我们描述了CDIO的工程教育环境,并突出强调了与CDIO不同但同样可行的其它工程教育环境的模型。在特定环境中进行工程教育的原因是显而易见的,它效法职业实践,有助于学习技能,是应用在工程教育和高等教育中基于环境学习的理论和最好的实践。最后,本文结尾讨论了对于采取适当的环境开展工程教育、更有效地培养工程师的广泛社会需求。突出阐述了中国工程教育的发展,同时也介绍了瑞典和美国对工程教育的需求。 | Edward F.Crawley 查建中 Johan Malmqvist Doris R.Brodeur | 2008 | 高等工程教育研究2008，56，4： | 124 |
| 2 | Gut microbiota imbalance and colorectal cancer显示文摘The gut microbiota acts as a real organ. The symbiotic interactions between resident micro-organisms and the digestive tract highly contribute to maintain the gut homeostasis. However, alterations to the microbiome caused by environmental changes(e.g., infection, diet and/or lifestyle) can disturb this symbiotic relationship and promote disease, such as inflammatory bowel diseases and cancer. Colorectal cancer is a complex association of tumoral cells, non-neoplastic cells and a large amount of micro-organisms, and the involvement of the microbiota in colorectal carcinogenesis is becoming increasingly clear. Indeed, many changes in the bacterial composition of the gut microbiota have been reported in colorectal cancer, suggesting a major role of dysbiosis in colorectal carcinogenesis. Some bacterial species have been identified and suspected to play a role in colorectal carcinogenesis, such as Streptococcus bovis, Helicobacter pylori, Bacteroides fragilis, Enterococcus faecalis, Clostridium septicum, Fusobacterium spp. and Escherichia coli. The potential pro-carcinogenic effects of these bacteria are now better understood. In this review, we discuss the possible links between the bacterial microbiota and colorectal carcinogenesis, focusing on dysbiosis and the potential pro-carcinogenic properties of bacteria, such as genotoxicity and other virulence factors, inflammation, host defenses modulation, bacterial derived metabolism, oxidative stress and anti-oxidative defenses modulation. We lastly describe how bacterial microbiota modifications could represent novel prognosis markers and/or targets for innovative therapeutic strategies. | Johan Gagnière Jennifer Raisch Julie Veziant Nicolas Barnich Richard Bonnet Emmanuel Buc Marie-Agnès Bringer Denis Pezet Mathilde Bonnet | 2016 | World Journal of Gastroenterology2016，22，2： | 76 |
| 3 | Management of acute nonvariceal upper gastrointestinal bleeding:Current policies and future perspectives显示文摘Acute upper gastrointestinal bleeding(UGIB) is a gastroenterological emergency with a mortality of 6%-13%.The vast majority of these bleeds are due to peptic ulcers.Nonsteroidal anti-inflammatory drugs and Helicobacter pylori are the main risk factors for peptic ulcer disease.Endoscopy has become the mainstay for diagnosis and treatment of acute UGIB,and is recommended within 24 h of presentation.Proton pump inhibitor(PPI) administration before endoscopy can downstage the bleeding lesion and reduce the need for endoscopic therapy,but has no effect on rebleeding,mortality and need for surgery.Endoscopic therapy should be undertaken for ulcers with high-risk stigmata,to reduce the risk of rebleeding.This can be done with a variety of modalities.High-dose PPI administration after endoscopy can prevent rebleeding and reduce the need for further intervention and mortality,particularly in patients with high-risk stigmata. | Ingrid Lisanne Holster Ernst Johan Kuipers | 2012 | World Journal of Gastroenterology2012，18，11： | 53 |
| 4 | Clinical approaches to non-alcoholic fatty liver disease显示文摘Non-alcoholic fatty liver disease(NAFLD)ranges from simple steatosis to nonalcoholic steatohepatitis(NASH),leading to fibrosis and potentially cirrhosis,and it is one of the most common causes of liver disease worldwide.NAFLD is associated with other medical conditions such as metabolic syndrome,obesity,cardiovascular disease and diabetes.NASH can only be diagnosed through liver biopsy,but noninvasive techniques have been developed to identify patients who are most likely to have NASH or fibrosis,reducing the need for liver biopsy and risk to patients.Disease progression varies between individuals and is linked to a number of risk factors.Mechanisms involved in the pathogenesis are associated with diet and lifestyle,influx of free fatty acids to the liver from adipose tissue due to insulin resistance,hepatic oxidative stress,cytokines production,reduced very low-density lipoprotein secretion and intestinal microbiome.Weight loss through improved diet and increased physical activity has been the cornerstone therapy of NAFLD.Recent therapies such as pioglitazone and vitamin E have been shown to be beneficial.Omega 3 polyunsaturated fatty acids and statins may offer additional benefits.Bariatric surgery should be considered in morbidly obese patients.More research is needed to assess the impact of these treatments on a long-term basis.The objective of this article is to briefly review the diagnosis,management and treatment of this disease in order to aid clinicians in managing these patients. | Katherine JP Schwenger Johane P Allard | 2014 | World Journal of Gastroenterology2014，20，7： | 21 |
| 5 | 比利时高放废物处置库设计及与基岩和工程屏障体系的热–水–力性状的相关研究(英文)显示文摘在比利时,泥岩中地质处置是高放废物最终处置的首选。处置库在高放废物与生物圈之间的多重屏障基础上设计的,而 Boom 泥岩作为基岩的研究已有 20 多年历史。1980 年比利时做出重大决定,建立名为 HADES 的地下研究机构,以研究 Boom 泥岩在地下 223 m 处的力学性质,并调查和论证处置的可行性,为处置库屏障(天然和人工)提供可靠数据。在 HADES 的众多现场试验中,很多试验用来对基岩和工程屏障体系(包括封口和回填的可行性)的热–水–力性状进行研究,包括 CACTUS,ATLAS,BACCHUS 和 RESEAL 等项目。自 1995 年以来,研究开发计划向大型和示范性试验方向发展。最主要成果是运用工业技术建立地下研究设施(竖井和井巷)可行性得到了验证,且这种工业技术给研究提供一个较好机会,便于进一步认识基岩泥岩(CLIPEX 方案)的水–力性状及了解隧道开挖工程(SELFRAC 课题)对挖掘破坏区的影响。另一个重大成果是成功地实现对一种称为“OPHELE”的预制膨润土(人工屏障材料)加热和水化地面大型试验。下一步工作内容包括实现大尺寸现场加热器试验(PRACLAY 试验),此试验预计于 2006 年开始,并可持续 10 a 之久。据此,首先简要描述比利时高放废物处置库设计,然后回顾 Boom 泥岩和工程屏障体系的热–水–力性状相关试验,最后介绍下一步大规模 PRACLAY 试验。 | 李香绫 Bernier Frédéric Bel Johan | 2006 | 岩石力学与工程学报2006，25，4： | 20 |
| 6 | Evolutionary History of the Non-Specific Lipid Transfer Proteins显示文摘非特定的类脂化合物转移蛋白质(nsLTPs ) 小、基本蛋白质由一个像隧道的恐水病的洞被描绘,能够转移在类脂化合物 bilayers 之间的各种各样的类脂化合物分子。大多数 nsLTPs 与本地化蛋白质到 apoplastic 空格的 N 终端信号肽被综合。nsLTPs 仅仅在种子植物被识别了,在他们被大基因家庭编码的地方。我们从非种子陆地植物和绿水藻用 genomic 和 EST 信息开始了 nsLTP 家庭的进化历史的分析决定:(1 ) 当 nsLTP 家庭产生了时,(2 ) 经常新的 nsLTP 亚科怎么被创造了,并且(3 ) 亚科怎么在不同植物系在他们扩大和损失的模式不同。在这研究,我们寻找了顺序数据库并且发现编码 nsLTPs 的基因和抄本充满地钱,苔藓,和另外的调查陆地种的所有,然而并非在任何水藻介绍。代表性的地钱和苔藓 nsLTPs 的第三级的结构进一步与相同建模被学习。在植物征服了土地以后,结果显示 nsLTP 家庭演变。仅仅, nsLTPs 的四个主要亚科中的二个在 flowering 植物发现了在苔藓和地钱是在场的。另外的亚科以后产生了,在陆地植物进化期间。在这份报告,我们也介绍一个修改 nsLTP 分类系统。 | Monika M. Edstam Lenita Viitanen Tiina A. Salminen Johan Edqvist | 2011 | Molecular Plant2011，4，6： | 18 |
| 7 | Minimally invasive surgery for gastric cancer: A comparison between robotic, laparoscopic and open surgery显示文摘AIM To investigate the role of minimally invasive surgery for gastric cancer and determine surgical, clinical, and oncological outcomes.METHODS This is a propensity score-matched case-control study, comparing three treatment arms: robotic gastrectomy(RG), laparoscopic gastrectomy(LG), open gastrectomy(OG). Data collection started after sharing a specific study protocol. Data were recorded through a tailored and protected web-based system. Primary outcomes: harvested lymph nodes, estimated blood loss, hospital stay, complications rate. Among the secondary outcomes, there are: operative time, R0 resections, POD of mobilization, POD of starting liquid diet and soft solid diet. The analysis includes the evaluation of type and grade of postoperative complications. Detailed information of anastomotic leakages is also provided.RESULTS The present analysis was carried out of 1026 gastrectomies. To guarantee homogenous distribution of cases, patients in the RG, LG and OG groups were 1:1:2 matched using a propensity score analysis with a caliper = 0.2. The successful matching resulted in a total sample of 604 patients(RG = 151; LG = 151; OG = 302). The three groups showed no differences in all baseline patients characteristics, type of surgery(P = 0.42) and stage of the disease(P = 0.16). Intraoperative blood loss was significantly lower in the LG(95.93 ± 119.22) and RG(117.91 ± 68.11) groups compared to the OG(127.26 ± 79.50, P = 0.002). The mean number of retrieved lymph nodes was similar between the RG(27.78 ± 11.45), LG(24.58 ± 13.56) and OG(25.82 ± 12.07) approach. A benefit in favor of the minimally invasive approaches was found in the length of hospital stay(P < 0.0001). A similar complications rate was found(P = 0.13). The leakage rate was not different(P = 0.78) between groups.CONCLUSION Laparoscopic and robotic surgery can be safely performed and proposed as possible alternative to open surgery. The main highlighted benefit is a faster postoperative functional recovery. | Amilcare Parisi Daniel Reim Felice Borghi Ninh T Nguyen Feng Qi Andrea Coratti Fabio Cianchi Maurizio Cesari Francesca Bazzocchi Orhan Alimoglu Johan Gagnière Graziano Pernazza Simone D'Imporzano Yan-Bing Zhou Juan-Santiago Azagra Olivier Facy Steven T Brower Zhi-Wei Jiang Lu Zang Arda Isik Alessandro Gemini Stefano Trastulli Alexander Novotny Alessandra Marano Tong Liu Mario Annecchiarico Benedetta Badii Giacomo Arcuri Andrea Avanzolini Metin Leblebici Denis Pezet Shou-Gen Cao Martine Goergen Shu Zhang Giorgio Palazzini Vito D'Andrea Jacopo Desiderio | 2017 | World Journal of Gastroenterology2017，23，13： | 17 |
| 8 | 对气候变暖响应下土壤CO_2释放的估计过高——老龄土壤有机质的分解具耐温性显示文摘本研究预测,相对于植被的净初级生产力(NPP)来说,随着全球变暖,有机质分解速度加快,土壤有机碳的贮藏速度将减少。因为土壤碳的含量是目前大气含量的两倍,而且从土壤中分解的碳要释放到大气中,因此,土壤碳贮量的减少会大量增加大气CO_2浓度。但是,土壤碳贮量减少的预测在很大程度上依赖于这样的隐含假设:所有土镶有机质的分解像新鲜凋落物的分解一样对温度较为敏感。我们测定了土壤碳的数量和年龄,以及沿温度梯度的凋落物的分解,并用一个简单的模型来描述土壤碳的动态,以显示老土壤有机质的分解阻抗温度的变化。因此,在对气候变暖的响应下,我们估算的分解速度的加快比从前预计的明显要低,并且预测北方森林土壤碳的贮量将增加。 | Jari Liski Annikki Mkel Carl Johan Westman 李叙勇 | 1999 | 人类环境杂志1999，28，2： | 16 |
| 9 | Ehealth:Low FODMAP diet vs Lactobacillus rhamnosus GG in irritable bowel syndrome显示文摘AIM:To investigate the effects of a low fermentable,oligosaccharides,disaccharides,monosaccharides and polyols diet(LFD)and the probiotic Lactobacillus rhamnosus GG(LGG)in irritable bowel syndrome(IBS).METHODS:Randomised,unblinded controlled trial on the effect of 6-wk treatment with LFD,LGG or a normal Danish/Western diet(ND)in patients with IBS fulfilling Rome III diagnostic criteria,recruited betweenNovember 2009 and April 2013.Patients were required to complete on a weekly basis the IBS severity score system(IBS-SSS)and IBS quality of life(IBS-QOL)questionnaires in a specially developed IBS web selfmonitoring application.We investigated whether LFD or LGG could reduce IBS-SSS and improve QOL in IBS patients.RESULTS:One hundred twenty-three(median age 37years,range:18-74 years),90(73%)females were randomised:42 to LFD,41 to LGG and 40 to ND.A significant reduction in mean±SD of IBS-SSS from baseline to week 6 between LFD vs LGG vs ND was revealed:133±122 vs 68±107,133±122 vs 34±95,P<0.01.Adjusted changes of IBS-SSS for baseline covariates showed statistically significant reduction of IBS-SSS in LFD group compared to ND(IBS-SSS score75;95%CI:24-126,P<0.01),but not in LGG compared to ND(IBS-SSS score 32;95%CI:18-80,P=0.20).IBS-QOL was not altered significantly in any of the three groups:mean±SD in LFD 8±18 vs LGG 7±17,LFD 8±18 vs ND 0.1±15,P=0.13.CONCLUSION:LFD is efficacious for patients with IBS. | Natalia Pedersen Nynne Nyboe Andersen Zsuzsanna Végh Lisbeth Jensen Dorit Vedel Ankersen Maria Felding Mette Hestetun Simonsen Johan Burisch Pia Munkholm | 2014 | World Journal of Gastroenterology2014，20，43： | 16 |
| 10 | Results of percutaneous sclerotherapy and surgical treatment in patients with symptomatic simple liver cysts and polycystic liver disease显示文摘AIM: To evaluate the results of the treatment of simple liver cysts (solitary and multiple) and polycystic liver disease (PLD) using percutaneous sclerotherapy and/or surgical procedures in a single tertiary referral centre. METHODS: Retrospective analysis of 54 patients referred for evaluation and possible treatment of simple liver cysts (solitary and multiple) and PLD, from January 1997 to July 2006. RESULTS: Simple liver cysts were treated in 41 pts (76/) with a mean size of 12.6 cm. The most common reason for referral was abdominal pain or discomfort (85/). Percutaneous sclerotherapy was performed as initial treatment in 30 pts, showing cyst recurrence in 6 pts (20/). Surgical treatment was initially performed in 11 pts with cyst recurrence in 3 pts (27/). PLD was treated in 13 pts (24/) with a mean size of the dominant cyst of 13 cm. Percutaneous sclerotherapy for PLD was performed in 9 pts with recurrence in 7 pts (77.8/). Surgical treatment for PLD was undertaken in 4 pts (30.8/) with recurrence in all. Eventually, 2 pts with PLD in the presence of polycystic kidney disease underwent liver-and kidney transplantation because of deterioration of liver and kidney function. CONCLUSION: The majority of patients with simple liver cysts and PLD are referred for progressive abdominal pain. As initial treatment, percutaneous sclerotherapy is appropriate. Surgical deroofing is indicated in caseof cyst recurrence after percutaneous sclerotherapy. However, the results of percutaneous sclerotherapy and surgical treatment for PLD are disappointing. Partial liver resection is indicated when there is suspicion of a pre-malignant lesion. | Deha Erdogan Otto M van Delden Erik AJ Rauws Olivier RC Busch Johan S Lameris Dirk J Gouma Thomas M van Gulik | 2007 | World Journal of Gastroenterology2007，13，22： | 15 |
| 11 | Low-FODMAP diet reduces irritable bowel symptoms in patients with inflammatory bowel disease显示文摘AIM To investigate the effect of a low-FODMAP diet on irritable bowel syndrome(IBS)-like symptoms in patients with inflammatory bowel disease(IBD).METHODS This was a randomised controlled open-label trial of patients with IBD in remission or with mild-to-moderate disease and coexisting IBS-like symptoms(Rome III) randomly assigned to a Low-FODMAP diet(LFD) or a normal diet(ND) for 6 wk between June 2012 andDecember 2013. Patients completed the IBS symptom severity system(IBS-SSS) and short IBD quality of life questionnaire(SIBDQ) at weeks 0 and 6. The primary end-point was response rates(at least 50-point reduction) in IBS-SSS at week 6 between groups; secondary end-point was the impact on quality of life. RESULTS Eighty-nine patients, 67(75%) women, median age 40, range 20-70 years were randomised: 44 to LFD group and 45 to ND, from which 78 patients completed the study period and were included in the final analysis(37 LFD and 41 ND). There was a significantly larger proportion of responders in the LFD group(n = 30, 81%) than in the ND group(n = 19, 46%);(OR = 5.30; 95%CI: 1.81-15.55, P < 0.01). At week 6, the LFD group showed a significantly lower median IBSSSS(median 115; inter-quartile range [IQR] 33-169) than ND group(median 170, IQR 91-288), P = 0.02. Furthermore, the LFD group had a significantly greater increase in SIBDQ(median 60, IQR 51-65) than the ND group(median 50, IQR 39-60), P < 0.01.CONCLUSION In a prospective study, a low-FODMAP diet reduced IBS-like symptoms and increased quality of life in patients with IBD in remission. | Natalia Pedersen Dorit Vedel Ankersen Maria Felding Henrik Wachmann Zsuzsanna Végh Line Molzen Johan Burisch Jens Rikardt Andersen Pia Munkholm | 2017 | World Journal of Gastroenterology2017，23，18： | 15 |
| 12 | 黄芪对鲤头肾中巨噬细胞的增殖和一氧化氮产量的影响:离体研究(英文)显示文摘用密度梯度离心分离鲤头肾中的巨噬细胞和嗜中性粒细胞。用RPMI细胞培养液对分离的细胞进行原代培养,在细胞培养液中加入不同浓度的黄芪水提取液来研究黄芪对鲤头肾中免疫细胞非特异性免疫应答的影响。黄芪水提取液单独使用时能刺激鲤头肾中巨噬细胞的增殖,但不能刺激巨噬细胞的氮暴发活性。用黄芪和脂多糖(LPS)混合刺激巨噬细胞和中性粒细胞时,黄芪水提取液能显著提高脂多糖刺激所产生的一氧化氮的产量。研究结果表明,黄芪不仅能刺激巨噬细胞数量的增加,而且能协同脂多糖增强头肾中免疫细胞的功能,从而对机体的非特异性免疫起调节作用。 | 殷国俊 Geert Wiegertjes LI Yue-ming Johan Schrama Johan Verreth 徐跑 周洪琪 | 2004 | 水产学报2004，28，6： | 14 |
| 13 | Exhuming the Meso-Cenozoic Kyrgyz Tianshan and Siberian Altai-Sayan:A review based on low-temperature thermochronology显示文摘Thermochronological datasets for the Kyrgyz Tianshan and Siberian Altai-Sayan within Central Asia reveal a punctuated exhumation history during the Meso-Cenozoic.In this paper,the datasets for both regions are collectively reviewed in order to speculate on the links between the Meso-Cenozoic exhumation of the continental Eurasian interior and the prevailing tectonic processes at the plate margins.Whereas most of the thermochronological data across both regions document late Jurassic-Cretaceous regional basement cooling,older landscape relics and dissecting fault zones throughout both regions preserve Triassic and Cenozoic events of rapid cooling,respectively.Triassic cooling is thought to reflect the Qjangtang- Eurasia collision and/or rifting/subsidence in the West Siberian basin.Alternatively,this cooling signal could be related with the terminal terrane-amalgamation of the Central Asian Orogenic Belt.For the Kygyz Tianshan,late Jurassic-Cretaceous regional exhumation and Cenozoic fault reactivations can be linked with specific tectonic events during the closure of the Palaeo-Tethys and Neo-Tethys Oceans,respectively.The effect of the progressive consumption of these oceans and the associated collisions of Cimmeria and India with Eurasia probably only had a minor effect on the exhumation of the Siberian Altai-Sayan.More likely,tectonic forces from the east(present-day coordinates) as a result of the building and collapse of the Mongol-Okhotsk orogen and rifting in the Baikal region shaped the current Siberian Altai-Sayan topography.Although many of these hypothesised links need to be tested further,they allow a first-order insight into the dynamic response and the stress propagation pathways from the Eurasian margin into the continental interior. | Stijn Glorie Johan De Grave | 2016 | Geoscience Frontiers2016，7，2： | 13 |
| 14 | Implication of the intestinal microbiome in complications of cirrhosis显示文摘The intestinal microbiome(IM) is altered in patients with cirrhosis,and emerging literature suggests that this impacts on the development of complications.The Pub Med database was searched from January 2000 to May 2015 for studies and review articles on the composition,pathophysiologic effects and therapeutic modulation of the IM in cirrhosis.The following combination of relevant text words and MeS H terms were used,namely intestinal microbiome,microbiota,or dysbiosis,and cirrhosis,encephalopathy,spontaneous bacterial peritonitis,hepatorenal syndrome,variceal bleeding,hepatopulmonary syndrome,portopulmonary hypertension and hepatocellular carcinoma.The search results were evaluated for pertinence to the subject of IM and cirrhosis,as well as for quality of study design.The IM in cirrhosis is characterized by a decreased proportion of Bacteroides and Lactobacilli,and an increased proportion of Enterobacteriaceae compared to healthy controls.Except for alcoholic cirrhosis,the composition of the IM in cirrhosis is not affected by the etiology of the liver disease.The percentage of Enterobacteriaceae increases with worsening liver disease severity and decompensation and is associated with bacteremia,spontaneous bacterial peritonitis and hepatic encephalopathy.Lactulose,rifaximin and Lactobacillus-containing probiotics have been shown to partial y reverse the cirrhosis associated enteric dysbiosis,in conjunction with improvement in encephalopathy.The IM is altered in cirrhosis,and this may contribute to the development of complications associated with end-stage liver disease.Therapies such as lactulose,rifaximin and probiotics may,at least partially,reverse the cirrhosisassociated changes in the IM.This,in turn,may prevent or alleviate the severity of complications. | Mamatha Bhat Bianca M Arendt Venkat Bhat Eberhard L Renner Atul Humar Johane P Allard | 2016 | World Journal of Hepatology2016，8，27： | 13 |
| 15 | Gastric accommodation assessed by ultrasonography显示文摘胃的住所为在机能性消化不良的病理生理学的理解是重要的并且为在另外的混乱的症状产生也是相关的。术语胃的住所有至少三个不同意思:住所过程,调节反射,和住所反应。胃的住所过程是描述胃的分隔空间的尺寸怎么响应饭变化的复杂现象。电子 barostat 在估计胃的住所被认为标准答案。成像方法,包括的 MRI, SPECT,和 ultrasonography 可以也被使用,特别地在压力应答的病人,例如机能性消化不良病人,因为一个非侵略、不太导致压力的方法是赞成的。Ultrasonography 满足它不由自己弄歪的这些标准在压力应答的个人的生理的反应。 | Odd Helge Gilja Johan Lunding Trygve Hausken Hans Gregersen | 2006 | World Journal of Gastroenterology2006，12，18： | 12 |
| 16 | Colorectal anastomotic leakage: Aspects of prevention, detection and treatment显示文摘All colorectal surgeons are faced from time to time with anastomotic leakage after colorectal surgery. This complication has been studied extensively without a significant reduction of incidence over the last 30 years. New techniques of prevention, by innovative anastomotic techniques should improve results in the future, but standardization and 'teachability' should be guaranteed. Risk scoring enables intra-operative decision-making whether to restore continuity or deviate. Early detection can lead to reduction in delay of diagnosis as long as a standard system is used. For treatment options, no firm evidence is available, but future studies could focus on repair and saving of the anastomosis on the one hand or anastomotical breakdown and definitive colostomy on the other hand. | Freek Daams Misha Luyer Johan F Lange | 2013 | World Journal of Gastroenterology2013，19，15： | 11 |
| 17 | Differential expression of interleukin-1/Toll-like receptor signaling regulators in microscopic and ulcerative colitis显示文摘AIM:To investigate Toll-like receptor(TLR)signaling regulators in microscopic and ulcerative colitis patients.METHODS:Total RNA and microRNA were isolated from fresh frozen colonic biopsies of non-inflamed controls and patients with active or in-remission collagenous colitis(CC),lymphocytic colitis(LC),or ulcerative colitis(UC).We compared expressions of interleukin-1receptor-associated kinase(IRAK)-2,IRAK-M,interleukin(IL)-37,microRNA(miR)-146a,miR-155,and miR-21 using quantitative real time reverse transcription polymerase chain reaction.RESULTS:IRAK-M expression was increased in LC patients with active disease in histopathological remission(LC-HR;P=0.02)and UC patients(P=0.01),but no differences in IRAK-2 expression were detected compared to controls.miR-146a,-155 and-21 expressions were increased in LC-HR(P=0.04,0.07,and 0.004)and UC(P=0.02,0.04 and 0.03)patients.miR-146a and miR-21 expressions were significantly enhanced in UC patients compared to UC remission(UC-R;P=0.01and 0.04).Likewise,active CC patients showed significantly increased expression of miR-155(P=0.003)and miR-21(P=0.006).IL-37 expression was decreased in both CC(P=0.03)and LC(P=0.04)patients with a similar trend in UC patients but not statistically significant,whilst it was increased in UC-R patients compared to controls(P=0.02)and active UC(P=0.001).CONCLUSION:The identification of differentially expressed miRNAs,IL-37,and IRAK-M suggests different pathophysiologic mechanisms in various disease stages in LC,CC,and UC. | Sezin Gunaltay Nils Nyhlin Ashok Kumar Kumawat Curt Tysk Johan Bohr Olof Hultgren Elisabeth Hultgren Hornquist | 2014 | World Journal of Gastroenterology2014，20，34： | 11 |
| 18 | Discriminating mild from critical COVID-19 by innate and adaptive immune single-cell profiling of bronchoalveolar lavages显示文摘How the innate and adaptive host immune system miscommunicate to worsen COVID-19 immunopathology has not been fully elucidated.Here,we perform single-cell deep-immune profiling of bronchoalveolar lavage(BAL)samples from 5 patients with mild and 26 with critical COVID-19 in comparison to BALs from non-COVID-19 pneumonia and normal lung.We use pseudotime inference to build T-cell and monocyte-to-macrophage trajectories and model gene expression changes along them.In mild COVID-19,CD8^(+)resident-memory(TRM)and CD4^(+)T-helper-17(T_(H17))cells undergo active(presumably antigen-driven)expansion towards the end of the trajectory,and are characterized by good effector functions,while in critical COVID-19 they remain more naïve.Vice versa,CD4^(+)T-cells with T-helper-1 characteristics(TH1-like)and CD8^(+)T-cells expressing exhaustion markers(T_(EX)-like)are enriched halfway their trajectories in mild COVID-19,where they also exhibit good effector functions,while in critical COVID-19 they show evidence of inflammation-associated stress at the end of their trajectories.Monocyte-to-macrophage trajectories show that chronic hyperinflammatory monocytes are enriched in critical COVID-19,while alveolar macrophages,otherwise characterized by anti-inflammatory and antigen-presenting characteristics,are depleted.In critical COVID-19,monocytes contribute to an ATP-purinergic signaling-inflammasome footprint that could enable COVID-19 associated fibrosis and worsen disease-severity.Finally,viral RNA-tracking reveals infected lung epithelial cells,and a significant proportion of neutrophils and macrophages that are involved in viral clearance. | Els Wauters Pierre Van Mol Abhishek Dinkarnath Garg Sander Jansen Yannick Van Herck Lore Vanderbeke Ayse Bassez Bram Boeckx Bert Malengier-Devlies Anna Timmerman Thomas Van Brussel Tina Van Buyten Rogier Schepers Elisabeth Heylen Dieter Dauwe Christophe Dooms Jan Gunst Greet Hermans Philippe Meersseman Dries Testelmans Jonas Yserbyt Sabine Tejpar Walter De Wever Patrick Matthys CONTAGIOUS collaborators Johan Neyts Joost Wauters Junbin Qian Diether Lambrechts | 2021 | Cell Research2021，31，3： | 11 |
| 19 | Association of colorectal cancer with pathogenic Escherichia coli: Focus on mechanisms using optical imaging显示文摘AIM: To investigate the molecular or cellular mechanisms related to the infection of epithelial colonic mucosa by pks-positive Escherichia coli(E. coli) using optical imaging.METHODS: We choose to evaluate the tumor metabolic activity using a fluorodeoxyglucose analogue as 2-deoxyglucosone fluorescent probes and to correlate it with tumoral volume(mm^3). Inflammation measuring myeloperoxidase(MPO) activity and reactive oxygen species production was monitored by a bioluminescent(BLI) inflammation probe and related to histological examination and MPO levels by enzyme-linked immunosorbent assay(ELISA) on tumor specimens. The detection and quantitation of these two signals were validated on a xenograft model of human colon adenocarcinoma epithelial cells(HCT116) in nude mice infected with a pks-positive E. coli. The inflammatory BLI signal was validated intra-digestively in the colitisCEABAC10 DSS models, which mimicked Crohn's disease. RESULTS: Using a 2-deoxyglucosone fluorescent probe, we observed a high and specific HCT116 tumor uptake in correlation with tumoral volume(P = 0.0036). Using the inflammation probe targeting MPO, we detected a rapid systemic elimination and a significant increase of the BLI signal in the pks-positive E. coli-infected HCT116 xenograft group(P < 0.005). ELISA confirmed that MPO levels were significantly higher(1556 ± 313.6 vs 234.6 ± 121.6 ng/m L P = 0.001) in xenografts infected with the pathogenic E. coli strain. Moreover, histological examination of tumor samples confirmed massive infiltration of pks-positive E. coli-infected HCT116 tumors by inflammatory cells compared to the uninfected group. These data showed that infection with the pathogenic E. coli strain enhanced inflammation and ROS production in tumors before tumor growth. Moreover, we demonstrated that the intra-digestive monitoring of inflammation is feasible in a reference colitis murine model(CEABAC10/DSS).CONCLUSION: Using BLI and fluorescence optical imaging, we provided tools to better understand hostpathogen interactions at the early stage of disease, such as inflammatory bowel disease and colorectal cancer. | Julie Veziant Johan Gagnière Elodie Jouberton Virginie Bonnin Pierre Sauvanet Denis Pezet Nicolas Barnich Elisabeth Miot-Noirault Mathilde Bonnet | 2016 | World Journal of Clinical Oncology2016，7，3： | 10 |
| 20 | Prediction and diagnosis of colorectal anastomotic leakage: A systematic review of literature显示文摘Although many studies have focused on the preoperative risk factors of anastomotic leakage after colorectal surgery(CAL), postoperative delay in diagnosis is common and harmful. This review provides a systematic overview of all available literature on diagnostic tools used for CAL. A systematic search of literature was undertaken using Medline, Embase, Cochrane and Webof-Science libraries. Articles were selected when a diagnostic or prediction tool for CAL was described and tested. Two reviewers separately assessed the eligibility and level of evidence of the papers. Sixty-nine articles were selected(clinical methods: 11, laboratory tests: 12, drain fluid analysis: 12, intraoperative techniques:22, radiology: 16). Clinical scoring leads to early awareness of probability of CAL and reduces delay of diagnosis. C-reactive protein measurement at postoperative day 3-4 is helpful. CAL patients are characterized by elevated cytokine levels in drain fluid in the very early postoperative phase in CAL patients. Intraoperative testing using the air leak test allows intraoperative repair of the anastomosis. Routine contrast enema is not recommended. If CAL is clinically suspected, rectal contrast-computer tomography is recommended by a few studies. In many studies a 'no-test' control group was lacking, furthermore no golden standard for CAL is available. These two factors contributed to a relatively low level of evidence in the majority of the papers. This paper provides a systematic overview of literature on the available tools for diagnosing CAL. The study shows that colorectal surgery patients could benefit from some diagnostic interventions that can easily be performed in daily postoperative care. | Freek Daams Zhouqiao Wu Max Jef Lahaye Johannus Jeekel Johan Frederik Lange | 2014 | World Journal of Gastrointestinal Surgery2014，6，2： | 10 |
您所在的机构暂未开通此部分的功能，请联系管理员