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| 1 | STX5 Inhibits Hepatocellular Carcinoma Adhesion and Promotes Metastasis by Regulating the PI3K/mTOR Pathway显示文摘Background and Aims:Syntaxin 5(STX5)is a member of the syntaxin or target-soluble SNAP receptor(t-SNARE)fam-ily and plays a critical role in autophagy.However,its function and molecular mechanism in tumor cell migration are still un-known.The role of STX5 in influencing hepatocellular carci-noma(HCC)is an important topic in our research.Methods:By using quantitative reverse transcription polymerase chain reaction(qPCR),western blotting,and immunohistochemical analysis of RNA and protein in tissues,we comprehensively evaluated data sets from public databases and clinical patient cohorts for STX5.The correlation of STX5 expression with the clinicopathological characteristics of HCC patients were assessed.In addition,we predicted signal pathways from dif-ferentially expressed genes(DEGs)and the Cancer Genome Atlas(TCGA)databases,and confirmed the prediction using integrated transcriptome and RNA-seq.We further investi-gated the underlying mechanisms of STX5 in the migration and adhesion of HCC cells both in vitro and in vivo.Results:In the TCGA dataset and our patient cohort,STX5 levels were significantly higher in HCC tissues than in adjacent normal liver tissues.At the same time,high expression of STX5 pre-dicted worse prognosis in patients with liver cancer.High ex-pression of STX5 indicates the decrease of adhesion and the increase of migration of HCC cells,and the conversion of epi-thelial-mesenchymal transition(EMT)in vitro via PI3K/mTOR pathway activation.Conversely,when Sirolimus,a phospho-inositide 3-kinase(PI3K)/AKT/mechanistic target of rapa-mycin(mTOR)inhibitor acts on cells simultaneously,STX5 overexpression-mediated enhancement of HCC metastasis is reversed.Double-negative regulation of STX5 and mTOR further enhanced the inhibitory effect of STX5 on HCC me-tastasis.In vivo,STX5 knockdown inhibited the metastasis of HCC cells.Conclusions:Our study demonstrates a novel research result that STX5 promotes HCC metastasis through PI3K/mTOR pathway.We believe that combined inhibition of STX5 and mTOR is a potential treatment for effectively pro-longing patient survival and inhibiting HCC metastasis. | Bin Zhang Ziyin Zhao Youpeng Wang Tingting Guo Mingyang He Ge Guan Pai Peng Jinzhen Cai Bingyuan Zhang Xutao Liu Qiaoling Song | 2023 | Journal of Clinical and Translational Hepatology2023，11，3： | 1 |
| 2 | CH_4 dissociation on Co(0001): A density functional theory study显示文摘CH4 dissociation on Co(0001) surfaces is an important step, which has been investigated at the level of density functional theory. It is found that CH4 is unfavorable to adsorb on Co(0001), while CH4 favores to dissociate to CH3, CH2 and CH on Co(0001) surface by sequential dehydrogenation. In the whole process of CH4 dehydrogenation, CH4 dissociate to CH3 and H is the rate-determining step. The calculated results show that CH2 and CH exist mainly on Co(0001) surface, while the dehydrogenation of CH into C and H is difficult. | Wei Huang Lulu Sun Peide Han Jinzhen Zhao | 2012 | Journal of Natural Gas Chemistry2012，21，1： | 1 |
| 3 | Contributions of Extractives and Lignin to the Fractal Geometry of Inter-nal Wood Surfaces显示文摘Internal wood surfaces can be treated as fractals, which are between Euclidean geometry and complete randomness.The fractal dimension Dfs is very informative in investigating the roughness of the internal surfaces of wood. In this study, the watersorption isotherms, including adsorption and desorption isotherm, of untreated, benzene-alcohol extracted and delignified (after ben-zene-alcohol extracted) spruce (Cuninghamia lanceolata) were measured at 30℃. On the basis of these isotherms, the Dfs valueswere calculated by FHH equation, which is based on multimolecular sorption. The results showed that both groups of Dfs values (re-spectively calculated from adsorption and desorption isotherms) of untreated, benzene-alcohol extracted and delignified wood havesame order, that is, untreated > benzene-alcohol extracted ≈ delignified. Therefore, the conclusion can be made that the ben-zene-alcohol extractives have significant contribution to the fractal geometry of internal wood surfaces. Lignin also has influence onthe fractal geometry, but this influence is very small while compared with that of the extractives. Moreover, the Dfs values calculatedfrom adsorption isotherms are bigger than those from desorption isotherms. | Jinzhen Cao Lu Ning Zhao GuangjieCollege of Material Science and Technology, Beijing Forestry University, Beijing 100083, P.R. China | 2003 | Forestry Studies in China2003，5，1： | 0 |
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