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1篇 您的检索式：作者名="Jennifer A.Maynard"

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		题名	作者	年代	出处	被引量
	1	Bioproduced Proteins On Demand(Bio-POD)in hydrogels using Pichia pastoris显示文摘Traditional production of industrial and therapeutic proteins by eukaryotic cells typically requires large-scale fermentation capacity.As a result,these systems are not easily portable or reusable for on-demand protein production applications.In this study,we employ Bioproduced Proteins On Demand(Bio-POD),a F127-bisurethane methacrylate hydrogel-based technique that immobilizes engineered Pichia pastoris for preservable,on-demand production and secretion of medium-and high-molecular weight proteins(in this case,SEAP,α-amylase,and anti-HER2).The gel samples containing encapsulated-yeast demonstrated sustained protein production and exhibited productivity immediately after lyophilization and rehydration.The hydrogel platform described here is the first hydrogel immobilization using a P.pastoris system to produce recombinant proteins of this breadth.These results highlight the potential of this formulation to establish a cost-effective bioprocessing strategy for on-demand protein production.	Shuo-Fu Yuan Sierra M.Brooks Annalee W.Nguyen Wen-Ling Lin Trevor G.Johnston Jennifer A.Maynard Alshakim Nelson Hal S.Alper	2021	Bioactive Materials2021，6，8：	0

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