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您的检索式：作者名="Hal S.Alper"
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| 1 | Expanding beyond canonical metabolism:Interfacing alternative elements,synthetic biology,and metabolic engineering显示文摘Metabolic engineering offers an exquisite capacity to produce new molecules in a renewable manner.However,most industrial applications have focused on only a small subset of elements from the periodic table,centered around carbon biochemistry.This review aims to illustrate the expanse of chemical elements that can currently(and potentially)be integrated into useful products using cellular systems.Specifically,we describe recent advances in expanding the cellular scope to include the halogens,selenium and the metalloids,and a variety of metal incorporations.These examples range from small molecules,heteroatom-linked uncommon elements,and natural products to biomining and nanotechnology applications.Collectively,this review covers the promise of an expanded range of elemental incorporations and the future impacts it may have on biotechnology. | Kevin B.Reed Hal S.Alper | 2018 | Synthetic and Systems Biotechnology2018，3，1： | 2 |
| 2 | CRISPR-PIN:Modifying gene position in the nucleus via dCas9-mediated tethering显示文摘Spatial organization of DNA within the nucleus is important for controlling DNA replication and repair,genetic recombination,and gene expression.Here,we present CRISPR-PIN,a CRISPR/dCas9-based tool that allows control of gene Position in the Nucleus for the yeast Saccharomyces cerevisiae.This approach utilizes a cohesindockerin interaction between dCas9 and a perinuclear protein.In doing so,we demonstrate that a single gRNA can enable programmable interaction of nuclear DNA with the nuclear periphery.We demonstrate the utility of this approach for two applications:the controlled segregation of an acentric plasmid and the re-localization of five endogenous loci.In both cases,we obtain results on par with prior reports using traditional,more cumbersome genetic systems.Thus,CRISPR-PIN offers the opportunity for future studies of chromosome biology and gene localization. | Jyun-Liang Lin Holly Ekas Matthew Deaner Hal S.Alper | 2019 | Synthetic and Systems Biotechnology2019，4，2： | 0 |
| 3 | Bioproduced Proteins On Demand(Bio-POD)in hydrogels using Pichia pastoris显示文摘Traditional production of industrial and therapeutic proteins by eukaryotic cells typically requires large-scale fermentation capacity.As a result,these systems are not easily portable or reusable for on-demand protein production applications.In this study,we employ Bioproduced Proteins On Demand(Bio-POD),a F127-bisurethane methacrylate hydrogel-based technique that immobilizes engineered Pichia pastoris for preservable,on-demand production and secretion of medium-and high-molecular weight proteins(in this case,SEAP,α-amylase,and anti-HER2).The gel samples containing encapsulated-yeast demonstrated sustained protein production and exhibited productivity immediately after lyophilization and rehydration.The hydrogel platform described here is the first hydrogel immobilization using a P.pastoris system to produce recombinant proteins of this breadth.These results highlight the potential of this formulation to establish a cost-effective bioprocessing strategy for on-demand protein production. | Shuo-Fu Yuan Sierra M.Brooks Annalee W.Nguyen Wen-Ling Lin Trevor G.Johnston Jennifer A.Maynard Alshakim Nelson Hal S.Alper | 2021 | Bioactive Materials2021，6，8： | 0 |
| 4 | Metabolic pathway engineering显示文摘Organisms can be engineered to produce a wide variety of compounds by either enhancing endogenous metabolic pathways,or by introducing exogenous pathways that are either borrowed from other organism,or de novo-designed pathways unknown to nature.While overexpression of bottleneck enzymes and deletion of competing pathways remain at the core of metabolic pathway engineering,there are many other key elements that need to be considered to successfully develop strains for the production of valuable products. | Hal S.Alper JoséL.Avalos | 2018 | Synthetic and Systems Biotechnology2018，3，1： | 0 |
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