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| 1 | Molecular diagnostics in a teacup:Non-Instrumented Nucleic Acid Amplification(NINA) for rapid,low cost detection of Salmonella enterica显示文摘We report on the use of a novel non-instrumented platform to enable a Loop Mediated isothermal Amplification(LAMP) based assay for Salmonella enterica.Heat energy is provided by addition of a small amount(<150 g) of boiling water,and the reaction temperature is regulated by storing latent energy at the melting temperature of a lipid-based engineered phase change material.Endpoint classification of the reaction is achieved without opening the reaction tube by observing the fluorescence of sequence-specific FRET-based assimilating probes with a simple handheld fluorometer.At or above 22℃ ambient temperature the non-instrumented devices could maintain reactions above a threshold temperature of 61℃ for over 90 min-significantly longer than the 60 min reaction time.Using the simple format,detection limits were less than 20 genome copies for reactions run at ambient temperatures ranging from 8 to 36℃.When used with a pre-enrichment step and non-instrumented DNA extraction device,trace contaminations of Salmonella in milk close to 1 CFU/mL could be reliably detected.These findings illustrate that the non-instrumented amplification approach is a simple,viable,low-cost alternative for field-based food and agricultural diagnostics or clinical applications in developing countries. | KUBOTA Ryo LABARRE Paul WEIGL Bernhard H LI Yong HAYDOCK Paul JENKINS Daniel M | 2013 | Chinese Science Bulletin2013，58，10： | 4 |
| 2 | Identification of Differentially Expressed Genes Associated with Cotton Fiber Development in a Chromosomal Substitution Line(CS-B22sh)显示文摘One of the impediments in the genetic improvement of cotton fiber is the paucity of information about genes associated with fiber development.Availability of chromosome arm substitution line CS- | SOLIMAN Khairy M BOLTON James J SAHA Sukumar JENKINS Johnie N | 2008 | 棉花学报2008，20，S1： | 4 |
| 3 | Durability Properties of Materials and Mixture Composition of Bitumen Stabilized Materials显示文摘Bitumen Stabilized Materials (BSMs) are widely recognized as environmentally friendly through significantly reduced energy consumption,reduced emissions and reuse of high percentage of RAP and recycled pavement materials. In order for these materials to be cost effective,their life-cycle performance needs to be proven. Durability of BSMs is a long-term phenomena that requires consideration during mix design. Time is very important variable for performance consideration under field conditions. The factors that influence durability and long-term performance of BSMs include the mineral aggregates,binder and mixture compositions. In this study,the important,but less understood aspects of durability properties of BSMs were investigated. These include wear and tear of mineral aggregates,age-hardening of binder (foamed bitumen and bitumen emulsion) and moisture susceptibility of typical mix composition. The wear and tear was investigated using Durability Mill Index (DMI) test,binder ageing was investigated for short and long-term effect,and moisture susceptibility was investigated by pulsing water pressures using new device i.e. moisture induction simulation test (MIST). The accelerated moisture conditioning simulates field conditions whereafter retained shear properties are evaluated. The results found that durability of mineral aggregates is significantly influences by the type and nature of mineral composition. The ageing of binder occurred in both short and long-term conditions,with short-term effects being dominating the foamed bitumen properties during laboratory production. Moisture susceptibility is significantly influenced by the mix compositions of aggregates blends with and without RAP,with different bitumen binder types (foamed bitumen or bitumen emulsion) and active filler type (cement or lime) and contents. The evaluation and ranking of mixes which are resistant and susceptible to moisture damage was done using retained cohesion (RC) after dry and wet monotonic triaxial testing. | TWAGIRA M E JENKINS J K | 2010 | 武汉理工大学学报2010，32，17： | 2 |
| 4 | The influence of amylose on starch granule structure显示文摘 | Jenkins P J Donld A M | 1995 | Int J Biol Macromol1995，17，19： | 2 |
| 5 | Pyrochemical reduction of uranium dioxide and plutonium dioxide by lithium metal显示文摘 | T Usami M Kurata T Inoue H.E Sims S.A Beetham J.A Jenkins | 2002 | Journal of Nuclear Materials2002，，1： | 2 |
| 6 | A semi-quantitative method for measuring Crptosporidium parvum infection using polymerase chain reaction显示文摘 | Jenkins M Trout J Fayer R | 1997 | J Microbiol Methods1997，28，2： | 1 |
| 7 | Novel 1xN and NxN Integrated Optical Switches Using Self-lmaging Multimode GaAs/Al-GaAs Waveguides显示文摘 | Jenkins R M Heaton J M Wright D R Parker J T Birbeck J C H Smith G W Hilton K P | 1994 | Appl Phys Lett 71994，64，： | 1 |
| 8 | Subpectoral breast reconstruction using the biodimensional system 显示文摘 | Jenkins M P | 1997 | Ann R Coll Surg Engl1997，79，： | 1 |
| 9 | Postpattum depression and apical balloning syndrome显示文摘 | YAQUB Y JENKIN L A NUGENT K M | 2009 | J Obstet Gynaeclo Can2009，31，： | 1 |
| 10 | Secondmeal effect:low-glycemic-index foods eaten at dinner improve subsequent breakfast glycemic response显示文摘 | Wolever T M Jenkins D J Ocana A M | 1988 | Am J Clin Nutr1988，48，： | 1 |
| 11 | A taxonomy for multi-Agent robotics 显示文摘 | DUDEK G JENKIN M R M MILIOS E | 1996 | Autonomous Robots1996，4，3： | 1 |
| 12 | Eimeria acerbulian:cloning if a cDNA encoding animmunogenic region of several related meroziote surface and rhoptry proteins显示文摘 | JENKINS M C | 1990 | Expermental Parasitology1990，70，： | 1 |
| 13 | Combustion-properties of biomass 显示文摘 | Jenkins B M Baxter L L Miles T R | 1998 | Fuel Processing Technology1998，54，： | 1 |
| 14 | Protective immunization against the intestinal parasite Eimeria accervulina with recombinant coccidial against antigen显示文摘 | Jenkins M C Siberian P C Augustine P C | 1991 | Poultry Science1991，70，： | 1 |
| 15 | Closure of patent foramen ovale for paradoxical emboli: intermediate-term risk of recurrent neurological events following transcatheter device placement 显示文摘 | Hung J Landzberg M J Jenkins K J | 2000 | J Am Coll Cardiol2000，35，5： | 1 |
| 16 | Viscous and nonvis- cous fibres, nonabsorbable and low glycaemic index carbohy- drates, blood lipids and coronary heart disease显示文摘 | Jenkins D J Kendall C W Axelsen M | 2000 | Curr Opin Lipidol2000，11，1： | 1 |
| 17 | Eimeria acervulina: DNA cloning and characterization of recombinant sporozoite and merozoite antigens显示文摘 | Jenkins M C Lillehoj H S Dame J B | | Exp Parasitol0，66，1： | 1 |
| 18 | Increased tissue kallikrein levels in type 2 di- abetes显示文摘 | CAMPBELL DJ KLADIS A ZHANG Y JENKINS AJ PRIOR DL YH M | 2010 | Diabetologia2010，53，4： | 1 |
| 19 | Metabolite control overrides circadian regulation of phosphoenelpyruvate carboxylase kinase and CO2 fixation in crassulacean acid metabolism 显示文摘 | Borland A M Hartwell J Jenkins G I | 1999 | Plant Physiol1999，121，： | 1 |
| 20 | Cloning and Expression of a DNA Sequence Encoding a 41-Kilodalton Cryptosporidium parvum Oocyst wall protein 显示文摘 | Jenkins M C Trout J Murphy C | 1999 | Clin Diagn Lab Immunol1999，6，6： | 1 |
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