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| 1 | “灰区”慢性乙型肝炎患者的分布及临床特征显示文摘【据Journal of Viral Hepatitis 2021年7月报道】题:“灰区”慢性乙型肝炎患者的分布及临床特征(作者Yao KF等)临床上存在相当一部分慢性乙型肝炎(CHB)患者的免疫状态无法分期,被认为处于“灰区”(GZ)。本研究旨在探究“灰区”CHB患者的分布及其临床特征。来自南京大学医学院附属鼓楼医院感染科的Yao等回顾性纳入了2016年1月-2020年8月未接受抗病毒治疗的CHB患者4759例。根据2018年美国肝病学会乙型肝炎防治指南对入组患者的免疫状态进行评判。 | YAO KF LIU JC WANG J 姚可方 黄睿 吴超 | 2021 | 临床肝胆病杂志2021，37，10： | 33 |
| 2 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 3 | 血压变异性预测自发性脑出血患者的院内不良预后显示文摘越来越多的证据表明,较高的收缩压变异性(systolicblood pressure variability ,SBPV)可能与脑出血患者的不良预后相关。该研究探讨了 SB PV 与院内脑出血预后之间的关系。研究者在2个医疗系统中收集了10年的自发性脑出血患者的连续数据。记录人口统计学、病史、实验室测试、计算机断层扫描数据、院内治疗情况以及神经和功能评估。在入院后24h内获取血压记录。 | 刘青 叶鹏 Divani AA Liu X Di Napoli M Lattanzi S Ziai W James ML Jafarli A Jafari M Saver JL Hemphill JC Vespa PM Mayer SA Petersen A | 2019 | 中华高血压杂志2019，27，8： | 6 |
| 4 | Salvage gamma knife stereotactic radiosurgery followed by bevacizumab for recurrent glioblastoma multiforme: a case-control study显示文摘We evaluated the efficacy and safety of gamma knife stereotactic radiosurgery(GKSR)followed by bevacizumab combined withchemotherapy in 11 patients with recurrent glioblastoma multiforme who experienced tumor progression despite aggressive | Park KJ Kano H Iyer A Liu X Niranjan A Flickinger JC Lieberman FS Lunsford LD Kondziolka D | 2012 | 中国神经肿瘤杂志2012，10，4： | 3 |
| 5 | Intracerebroventricular leptin regulates hepatic but not peripheral glucose fluxes 显示文摘 | Liu L Karkanias GB Morales JC | 1998 | J Biol Chem1998，273，31： | 1 |
| 6 | Mesenteric fat thickness as an independent determinant of fatty liver显示文摘 | Liu KH Chan YL Chan JC | 2006 | Int J Obes(Lond)2006，30，5： | 1 |
| 7 | Maternal care,hippocampal synaptogenesis and cognitive development in rats显示文摘 | Liu D Diorio J Day JC | 2000 | Nat Neurosci2000，3，8： | 1 |
| 8 | The incidence of cerebrospinal fluid leak after vestibular schwannoma surgery显示文摘 | Selesnick SH Liu JC Jen A | 2004 | Otol Neurotol2004，25，3： | 1 |
| 9 | Control of inducible chemoresistance: enhanced anti-tumor therapy through increased apoptosis by inhibition of NF-kappaB 显示文摘 | Wang CY Cusack JC Jr Liu R | 1999 | Nat Med1999，5，4： | 1 |
| 10 | Neuronal mitochondrial a-melioration by feeding acetyl-L-carnitine and lipoic acid toaged rats显示文摘 | Aliev G Liu J Shenk JC | 2009 | J Cell Mol Med2009，13，2： | 1 |
| 11 | A multiplex degenerate PCR analytical approach targeting to eight genes for screening GMOs显示文摘 | Guo JC Chen LL Liu X | | 0，，03： | 1 |
| 12 | Sleep Apnea and Risk of Peptic Ulcer Bleeding: A Nationwide Population-based Study 显示文摘 | Shiao TH Liu C J Luo JC | 2013 | American Journal of Medicine2013，126，3： | 1 |
| 13 | Comparison of ultrasound and electromagnetic field effects on osteoblast growth 显示文摘 | Li JK Lin JC Liu HC | 2006 | Ultrasound Med Biol2006，32，： | 1 |
| 14 | Anti-arrhythmic effects of K-opioid receptor anti its changes in ischemia and reperfusion显示文摘 | Liu JC Yin W Yin Z | 2008 | Arch Med Res2008，39，5： | 1 |
| 15 | Antioxidant action via p53-mediated apoptosis显示文摘 | Liu M Pelling JC Ju J | 1998 | Cancer Res1998，58，8： | 1 |
| 16 | Improved dual-energymaterial discrimination for dual-source CT by means of additionalspectral filtration显示文摘 | Primak AN Ramirez Giraldo JC Liu X | 2009 | Med Phys2009，36，4： | 1 |
| 17 | Prognostic factors affecting the outcome of early cervical cancer treated with radical hysterectomy and post-operative adjuvant therapy显示文摘 | Liu MT Hsu JC Liu WS | 2008 | Eur J Cancer Care(Engl)2008，17，2： | 1 |
| 18 | Erk associates with and primes GSK-3beta for its inactivation resulting in upregulation of beta-catenin显示文摘 | Ding Q Xia W Liu JC | 2005 | Mol Cell2005，19，2： | 1 |
| 19 | The effect of repeated stretching on the force decay and compliance of vu- lcanized cis-polyisoprene orthodontic elastics显示文摘 | Liu CC Wataha JC Craig RG | 1993 | Dent Mater1993，9，1： | 1 |
| 20 | New stereotac- tic technique for percutaneous thermocoagulation upper thoracic ganglionectomy in cases ofpalmar hyperhidrosis 显示文摘 | Chuang KS Liou NH Liu JC | 1988 | Neurosurgery1988，22，： | 1 |
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