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| 1 | Epidemiology of lung cancer and approaches for its prediction:a systematic review and analysis显示文摘Background: Owing to the use of tobacco and the consumption of alcohol and adulterated food, worldwide cancer incidence is increasing at an alarming and frightening rate. Since the last decade of the twentieth century, lung cancer has been the most common cancer type. This study aimed to determine the global status of lung cancer and to evaluate the use of computational methods in the early detection of lung cancer.Methods: We used lung cancer data from the United Kingdom(UK), the United States(US), India, and Egypt. For statistical analysis, we used incidence and mortality as well as survival rates to better understand the critical state of lung cancer.Results: In the UK and the US, we found a significant decrease in lung cancer mortalities in the period of 1990–2014, whereas, in India and Egypt, such a decrease was not much promising. Additionally, we observed that, in the UK and the US, the survival rates of women with lung cancer were higher than those of men. We observed that the data mining and evolutionary algorithms were efficient in lung cancer detection.Conclusions: Our findings provide an inclusive understanding of the incidences, mortalities, and survival rates of lung cancer in the UK, the US, India, and Egypt. The combined use of data mining and evolutionary algorithm can be efficient in lung cancer detection. | Ashutosh Kumar Dubey Umesh Gupta Sonal Jain | 2016 | Chinese Journal of Cancer2016，35，9： | 37 |
| 2 | 中国2型糖尿病患者教育的长期成本效果评估显示文摘目的评价中国2型糖尿病患者教育的长期成本效果。方法使用瑞士结果研究中心(CORE)糖尿病模型,基于亚洲糖尿病治疗现状(Diabcare-Asia)调查项目中国调研(1998-2006)中2型糖尿病患者教育组和对照组的临床数据和其他公开发表的相关资料,模拟50年内糖尿病患者教育对中国2型糖尿病的疾病进程、医疗费用、患者生活质量等的影响。采用配比对照法将患者分为教育组(568名)和对照组(620名)。患者平均糖化血红蛋白(HbAlc)为7.6%±1.6%,病程为(3.2±5.6)年,平均年龄(55±11)岁,男性591例,女性597例。结果模拟结果显示接受过患者教育的2型糖尿病患者罹患严重并发症的风险降低:终末期肾病降低45.5%,糖尿病眼病降低30.9%,心肌梗死降低9.2%;预期寿命增加1.1年,质量调整生命年(QALY)增加0.76年,个人总医疗费用增加5183元;与对照组相比,接受过患者教育的患者增量成本效果比(ICER)为6784元/QALY。结论糖尿病患者教育能够提高2型糖尿病患者的预期寿命,降低糖尿病并发症的风险,并且是一项具有成本效果的干预措施。 | 郭晓蕙 Pavika Jain 于忱非 | 2012 | 中华糖尿病杂志2012，4，2： | 30 |
| 3 | Diagnosis of Helicobacter pylori : What should be the gold standard?显示文摘Since the discovery of Helicobacter pylori(H. pylori) in 1983, numerous detection methods for the presence of the bacterium have been developed. Each one of them has been associated with advantages and disadvantages. Noninvasive tests such as serology, 13 C urea breath test(UBT) and stool antigen tests are usually preferred by the clinicians. Serology has its own limitation especially in endemic areas while 13 C UBT is technically very demanding. The stool antigen detection method, although specific, is usually associated with poor sensitivity. The 13 C UBT is believed to be specific, but with present revelation of the fact that stomach is colonized by many other urease producing bacteria makes it questionable. Histology, culture, rapid urease test and polymerase chain reaction(PCR) are the tests which are carried out on antral biopsies collected by invasive means. Histology has been proposed to be very sensitive and specific but the question is how by simply looking the morphology of the bacteria in the microscope, one can claim that the curved bacterium is exclusively H. pylori. Rapid urease test(RUT), the doctor's test, is also challenged because the presence of other urease producing bacteria in the stomach cannot be denied. Moreover, RUT has been reported with poor sensitivity specially, when density of the bacterium is low. Isolation of H. pylori is essential to investigate its growth requirements, antibiotic susceptibility testing, studying virulence factor to develop vaccine and many more explorations. It has also got several disadvantages i.e., special condition for transporting, media, incubation and few days waiting for the colonies to appear, apart from the speed essentially needed to process the specimens. Till date, majority of the microbiological laboratories in the world are not equipped and trained to isolate such fastidious bacterium. The option left is PCR methods to detect H. pylori 's DNA in gastric mucosa, gastric juice, saliva, dental plaques and environmental specimens. There are speculations for false positivity due to detection of non-pylori Helicobacters due to genetic sharing; and false negativity due to low bacterial counts and presence of PCR inhibitors. However, specimen collection, transportation and processing do not require speed and special conditions. PCR based diagnosis may be considered as gold standard by designing primers extremely specific to H. pylori and targeting at least more than one conserved genes. Similarly specificity of PCR may be improved by use of internal Primers. Further, nested PCR will take care of false negatives by countering the effect of PCR inhibitors and low bacterial counts. Therefore, nested PCR based methods if performed properly, may be proposed as gold standard test. | Saurabh Kumar Patel Chandra Bhan Pratap Ashok Kumar Jain Anil Kumar Gulati Gopal Nath | 2014 | World Journal of Gastroenterology2014，20，36： | 30 |
| 4 | Outcome of open reduction and internal fixation of intraarticular calcaneal fracture fixed with locking cal- caneal plate显示文摘ObjectiveDebate 关于跟骨的破裂的管理继续,在开的减小和内部固定和关上的治疗之间。因此,我们瞄准在与锁跟骨的 plate.MethodsIn 修理的跟骨的破裂的代替的联合消沉类型评估开的减小和内部固定的放射学、功能的结果这个系列, 28 个病人( 26 单方并且 2 双边)与跟骨的破裂的联合消沉类型,作为每 Essex-Lopresti ,分类系统与在 3 星期损害以内锁跟骨的板被操作。病人们以 anteroposterior 的联系损害和 X 光被评估, calcaneum 的侧面、轴的看法。CT 扫描被做估计粉碎和关节的消沉的数量。病人们被跟随在上面临床上并且放射学地至少为 1 年。放射学的评价被 Bohler’s 角度和 Gissane’s 角度与跟骨的高度和宽度的测量一起做。功能的结果用 14.5 个月的美国 Orthopaedics 英尺和脚关节社会( AOFAS ) scale.ResultsAt 一般水准后续被估计,平均 AOFAS 分数是 86.3 (范围 66~97 ),与86%有对好结果优秀并且 2 (7.7%)并且 1 (3.7%)分别地有公平、差的结果。所有病人让稳定的脚关节与所有有的背屈和脚底的屈曲连接超过 30 °
。运动的平均 subtalar 范围是 17 °
。吝啬的 Bohler’s 角度,吝啬的 Gissane’s 角度,跟骨的高度和宽度是 25.47 °
, 121.3 °
, 4.32 厘米和 3.81 厘米分别地在最后的后续。三个病人在切口地点有拍动坏死,一个人有表面、深的感染。Subtalar 关节炎在 5 个病人被看见,而在 1 个病人的小腿腹的神经 hypoaesthesia。任何一个都没病人有分隔空间症候群,脚跟垫问题,小腿侧的 tendinitis,反射同情的 dystropy 或与锁住植入 failure.ConclusionOpen 减小和内部固定跟骨的板给声音功能的结果,即解剖地恢复高度,宽度, Bohler’s 和 calcaneum 的 Gissiane’s 角度的重建,并且允许早动员。 | Saurabh Jain Anti Kumar Jain Ish Kumar | 2013 | Chinese Journal of Traumatology2013，16，6： | 25 |
| 5 | Insulin and heparin in treatment of hypertriglyceridemia-induced pancreatitis显示文摘TO THE EDITOR We read with great interest the case report, 'Hypertriglyceridemia -induced pancreatitis: A case-based review' by Gan et al[1] in the November 2006 issue of World Journal of Gastroenterology. | Pankaj Jain Ramesh Roop Rai Harsh Udawat Sandeep Nijhawan Amit Mathur | 2007 | World Journal of Gastroenterology2007，13，18： | 23 |
| 6 | Human amniotic membrane transplantation: Different modalities of its use in ophthalmology显示文摘The amniotic membrane(AM) is the inner layer of the fetal membranes and consist of 3 different layers: the epithelium, basement membrane and stroma which further consists of three contiguous but distinct layers: the inner compact layer, middle fibroblast layer and the outermost spongy layer. The AM has been shown to have anti-inflammatory, anti-fibrotic, anti-angiogenic as well as anti-microbial properties. Also because of its transparent structure, lack of immunogenicity and the ability to provide an excellent substrate for growth, migration and adhesion of epithelial corneal and conjunctival cells, it is being used increasingly for ocular surface reconstruction in a variety of ocular pathologies including corneal disorders associated with limbal stem cell deficiency, surgeries for conjunctival reconstruction, as a carrier for ex vivo expansion of limbal epithelial cells, glaucoma surgeries and sceral melts and perforations. However indiscriminate use of human AM needs to be discouraged as complications though infrequent can occur. These include risk of transmission of bacterial, viral or fungal infections to the recipient if the donors are not adequately screened for communicable diseases, if the membrane is not processed under sterile condi-tions or if storage is improper. Optimal outcomes can be achieved only with meticulous case selection. This review explores the ever expanding ophthalmological indications for the use of human AM. | Chintan Malhotra Arun K Jain | 2014 | World Journal of Transplantation2014，4，2： | 21 |
| 7 | Delphi methodology in healthcare research:How to decide its appropriateness显示文摘The Delphi technique is a systematic process of forecasting using the collective opinion of panel members.The structured method of developing consensus among panel members using Delphi methodology has gained acceptance in diverse fields of medicine.The Delphi methods assumed a pivotal role in the last few decades to develop best practice guidance using collective intelligence where research is limited,ethically/logistically difficult or evidence is conflicting.However,the attempts to assess the quality standard of Delphi studies have reported significant variance,and details of the process followed are usually unclear.We recommend systematic quality tools for evaluation of Delphi methodology;identification of problem area of research,selection of panel,anonymity of panelists,controlled feedback,iterative Delphi rounds,consensus criteria,analysis of consensus,closing criteria,and stability of the results.Based on these nine qualitative evaluation points,we assessed the quality of Delphi studies in the medical field related to coronavirus disease 2019.There was inconsistency in reporting vital elements of Delphi methods such as identification of panel members,defining consensus,closing criteria for rounds,and presenting the results.We propose our evaluation points for researchers,medical journal editorial boards,and reviewers to evaluate the quality of the Delphi methods in healthcare research. | Prashant Nasa Ravi Jain Deven Juneja | 2021 | World Journal of Methodology2021，11，4： | 21 |
| 8 | Acute pancreatitis in pregnancy:An unresolved issue显示文摘Management of acute pancreatitis in pregnancy is based on expert opinion only,due to geographic and ethic variations.Nonbiliary causes should be sought as they are associated with worse outcomes.Alcohol as a cause of acute pancreatitis is not rare.Hemoconcentration as a marker of fluid def icit and severity should be predicted with caution and fluid resuscitation should be done carefully by closely monitoring the central venous pressure,cardiac and respiratory system.Hypercalcemia of hyperparathyroidism may be falsely lowered due to hypoalbuminemia or suppressed by magnesium tocolysis. | Pankaj Jain | 2010 | World Journal of Gastroenterology2010，16，16： | 17 |
| 9 | 分布式发电当前趋势与将来挑战(英文)显示文摘近年来国际上在可再生能源的研发方面做了大量的工作,促进了分布式发电在实际电力系统中得到越来越广泛的应用.综述了分布式发电技术,包括风力发电、太阳能、燃料电池等;阐述了不同分布式发电技术的动态模型,分析了分布式发电对电力系统运行控制及保护的影响.并探讨了增加电力系统分布式发电比例的挑战. | Naveen Jain S. N. Singh 文福拴 | 2008 | 电力科学与技术学报2008，23，4： | 16 |
| 10 | 肝移植术后晚期合并多器官功能衰竭主要死因分析显示文摘目的 探讨肝移植术后晚期导致患者死亡的主要原因 ,提出预防的方法。方法 回顾性分析1981年 2月— 1998年 4月在美国匹斯堡大学进行的生存期大于 1年的 2 94 0例肝移植长期随访结果 ,分析其主要死因与相关因素。结果 1981— 1991年 10年肝移植术后病死率呈逐年降低趋势 ,从 2 0 .4 %降到 1.9% ;术后晚期 (存活期 >1年 )死亡者 817例 ,主要死因依次为恶性肿瘤 (复发 +新生 ,2 0 .7% )、心血管并发症( 11.4 % )、各种感染 ( 11.3% )和呼吸系统并发症 ( 9.4 % )。随着免疫抑制剂使用情况的变化 ,1981— 1986年为环胞素 / Cy A,1986— 1990年为 OKT3/ Viaspan,1991— 1998年为 Tarolim us/ FK5 0 6 ,各期 1、5、10年病死率呈逐年下降趋势。术后 1、5、10、15和 18年病死率以 3~ 18岁年龄为低 ,19~ 6 0岁年龄组 10、15、18年病死率最高。病死率最高的肝移植指征为恶性肿瘤和暴发性肝功能衰竭 ,酒精性肝硬化术后随时间延长病死率逐年增加 ,13年最高 (达 76 .0 % )。结论 肝移植术后期主要死因为新生恶性肿瘤、心血管并发症、各种感染和呼吸系统并发症 ; | 潘澄 Ashok Jain John J.Fung | 2004 | 中国危重病急救医学2004，16，9： | 16 |
| 11 | Implant biomaterials: A comprehensive review显示文摘Appropriate selection of the implant biomaterial is a key factor for long term success of implants. The biologic environment does not accept completely any material so to optimize biologic performance, implants should be selected to reduce the negative biologic response while maintaining adequate function. Every clinician should always gain a thorough knowledge about thedifferent biomaterials used for the dental implants. This article makes an effort to summarize various dental biomaterials which were used in the past and as well as the latest material used now. | Monika Saini Yashpal Singh Pooja Arora Vipin Arora Krati Jain | 2015 | World Journal of Clinical Cases2015，3，1： | 15 |
| 12 | Safe laparoscopic cholecystectomy:Adoption of universal culture of safety in cholecystectomy显示文摘The incidence of biliary injury after laparoscopic cholecystectomy(LC) has shown a declining trend though it may still be twice that as with open cholecystectomy. Major biliary or vasculobiliary injury is associated with significant morbidity. As prevention is the best strategy, the concept of a culture of safe cholecystectomy has been recently introduced to educate surgeons and apprise them of basic tenets of safe performance of LC. Various aspects of safe cholecystectomy include:(1) thorough knowledge of relevant anatomy, various anatomical landmarks, and anatomical variations;(2) an understanding of the mechanisms involved in biliary/vascular injury, the most important being the misidentification injury;(3) identification of various preoperative and intraoperative predictors of difficult cholecystectomy;(4) proper gallbladder retraction;(5) safe use of various energy devices;(6) understanding the critical view of safety, including its doublet view and documentation;(7) awareness of various error traps(e.g., fundus first technique);(8) use of various bailout strategies(e.g., subtotal cholecystectomy) in difficult gallbladder cases;(9) use of intraoperative imaging techniques(e.g., intraoperative cholangiogram) to ascertain correct anatomy; and(10) understanding the concept of time-out.Surgeons should be facile with these aspects of this culture of safety in cholecystectomy in an attempt to reduce the incidence of biliary/vascular injury during LC. | Vishal Gupta Gaurav Jain | 2019 | World Journal of Gastrointestinal Surgery2019，11，2： | 15 |
| 13 | Gall bladder carcinoma: Aggressive malignancy with protean loco-regional and distant spread显示文摘The most common malignancy of biliary tract is gallbladder cancer(GBC) which is the third most common cancer in gastrointestinal tract. It is a lethal disease for most patients in spite of growing awareness and improved diagnostic techniques. GBC has a verypoor prognosis and the 5 year survival rate is < 10%. Although etiology of the carcinoma of the gallbladder is still obscure, various factors have been implicated, cholelithiasis being the most frequent. The incidence of GBC worldwide is based on the gender, geography and ethnicity which suggest that both genetic and environmental factors can cause GBC. The major route of spread of gallbladder cancer(GC) is locoregional rather than distant. It spreads by lymphatic, vascular, neural, intraperitoneal, and intraductal routes. Sonography is usually the most common imaging test to evaluate symptoms of biliary tract disease including suspected GC. With recent advances in imaging modalities like multi-detector computed tomography(CT) scanners, magnetic resonance imaging-positron emission tomography/CT diagnosis of gallbladder cancer has improved. Studies have also targeted molecular and genetic pathways. Treatment options have included extended and radical surgeries and adjuvant chemotherapy. This review article deals in detail with important aspects of carcinoma gallbladder and its manifestations and challenges. Role of various imaging modalities in characterization and accurate staging has been discussed. The loco-regional spread of this aggressive malignancy is dealt explicitly. | Amit Nandan Dhar Dwivedi Shivi Jain Ruhi Dixit | 2015 | World Journal of Clinical Cases2015，3，3： | 14 |
| 14 | Surgical treatment of proximal humerus fractures using PHILOS plate显示文摘ObjectiveTo 与经历了的 51 个病人打开的为近似肱骨 fractures.MethodsWe 的板考察了的近似肱骨的内部锁住系统(PHILOS ) 评估开的减小和内部固定的功能的结果和复杂并发症有在年之间的 PHILOS 板的减小和内部固定 2007 ~ 2012。与 38 的吝啬的年龄有 35 个男人和 16 个女人;年(范围 24-68 ) 。在年龄组有 41 个病人 < 60 在年龄组的年和 10 个病人 > 60 年。根据更旧姓的分类系统, 8, 15 和 23 个病人有 2 部分, 3 部分,和 4 部分破裂,分别地并且 5 个病人让 4 部分断裂脱臼。所有手术在我们的第三级的照顾损伤中心被执行。在最后的后续的肩膀的功能的评估用 Constant-Murley score.ResultsThe 平均数后续被做经期是 30 月(范围 12-44 月) 。二个病人被失去到后续。留下 49 个病人,所有破裂临床上并且放射学地被统一。放射学的联合的吝啬的时间是 12 星期(范围 8-20 星期) 。在最后的后续,吝啬的 Constant-Murley 分数是 79 (范围 50-100 ) 。结果在 25 个病人,是优秀的在 13 个病人好、在 6 个病人公平、在 5 个病人差。在后续期间,内翻足 malunion, subacromial 冲击的一个盒子,深感染的一个盒子, intraarticular 螺丝钉穿入的一个盒子和固定的失败的一个盒子的四个盒子被注意。avascular 坏死的没有盒子,硬件失败,松开的锁住的螺丝钉或不属于工会 noted.ConclusionPHILOS 在近似肱骨破裂提供稳定的固定。为了阻止潜在的复杂并发症,希望 avascular 坏死,保存肱骨的头的由脉管形成的过细的外科的解剖是必要的。 | GN Kiran Kumar Gaurav Sharma Vijay Sharma Vaibhav Jain Kamran Farooque Vivek Morey | 2014 | Chinese Journal of Traumatology2014，17，5： | 14 |
| 15 | 再次肝移植——挽救肝移植失败受体生命唯一的手段(附774例报告)显示文摘目的 评估肝移植 ,尤其是再次肝移植的长期随访结果及影响结果的因素。方法 对1981年 2月至 1998年 4月期间进行的、存活时间大于 2年的 40 0 0例肝移植进行随访 ,其中再次肝移植 774例。根据首次肝移植的时间 ,分为A、B、C三期。结果 774例 (19 4% )接受第 2次肝移植 ,148例 (3 7% )接受第 3次肝移植 ,2 0例 (0 5 % )接受第 4次肝移植 ,5例 (0 13% )接受第 5次及 5次以上肝移植。第 1次再移植原因主要为移植肝原发性无功能、肝动脉栓塞和排斥反应。C期再次肝移植率 (13 4% )明显低于A期 (33 4% )和B期 (2 3 7% ) ,P =0 0 0 1。结论 掌握适当的再移植指征、再次手术时机、受体的选择和手术技巧 ,再次肝移植的长期生存率明显改善。 | 朱岳 江春平 John J. Fung Ashok Jain | 2002 | 中华肝胆外科杂志2002，8，11： | 14 |
| 16 | Ciliary parathyroid hormone signaling activates transforming growth factor-βto maintain intervertebral disc homeostasis during aging显示文摘Degenerative disc disease(DDD) is associated with intervertebral disc degeneration of spinal instability. Here, we report that the cilia of nucleus pulposus(NP) cells mediate mechanotransduction to maintain anabolic activity in the discs. We found that mechanical stress promotes transport of parathyroid hormone 1 receptor(PTH1 R) to the cilia and enhances parathyroid hormone(PTH) signaling in NP cells. PTH induces transcription of integrin α_vβ_6 to activate the transforming growth factor(TGF)-β-connective tissue growth factor(CCN2)-matrix proteins signaling cascade. Intermittent injection of PTH(iPTH) effectively attenuates disc degeneration of aged mice by direct signaling through NP cells, specifically improving intervertebral disc height and volume by increasing levels of TGF-β activity, CCN2, and aggrecan. PTH1 R is expressed in both mouse and human NP cells. Importantly,knockout PTH1 R or cilia in the NP cells results in significant disc degeneration and blunts the effect of PTH on attenuation of aged discs. Thus, mechanical stress-induced transport of PTH1 R to the cilia enhances PTH signaling, which helps maintain intervertebral disc homeostasis, particularly during aging, indicating therapeutic potential of iPTH for DDD. | Liwei Zheng Yong Cao Shuangfei Ni Huabin Qi Zemin Ling Xin Xu Xuenong Zou Tianding Wu Ruoxian Deng Bo Hu Bo Gao Hao Chen Yusheng Li Jianxi Zhu Francis Tintani Shadpour Demehri Amit Jain Khaled M.Kebaish Shenghui Liao Cheryle A.Séguin Janet L.Crane Mei Wan Hongbin Lu Paul D.Sponseller Lee H.RileyIII Xuedong Zhou Jianzhong Hu Xu Cao | 2018 | Bone Research2018，6，3： | 13 |
| 17 | On-table reconstruction and fixation of Mason type III radial head fractures显示文摘 | G.N. Kiran Kumar Gaurav Sharma Kamran Farooque Vijay Sharma Vaibhav Jain Ravijot Singh Vivek Morey | 2015 | Chinese Journal of Traumatology2015，18，5： | 12 |
| 18 | Effective mass and Fermi surface complexity factor from ab initio band structure calculations显示文摘The effective mass is a convenient descriptor of the electronic band structure used to characterize the density of states and electron transport based on a free electron model.While effective mass is an excellent first-order descriptor in real systems,the exact value can have several definitions,each of which describe a different aspect of electron transport.Here we use Boltzmann transport calculations applied to ab initio band structures to extract a density-of-states effective mass from the Seebeck Coefficient and an inertial mass from the electrical conductivity to characterize the band structure irrespective of the exact scattering mechanism.We identify a Fermi Surface Complexity Factor:N_(v)^(*)K^(*) from the ratio of these two masses,which in simple cases depends on the number of Fermi surface pockets eN_(v)^(*) T and their anisotropy K^(*),both of which are beneficial to high thermoelectric performance as exemplified by the high values found in PbTe.The Fermi Surface Complexity factor can be used in high-throughput search of promising thermoelectric materials. | Zachary M.Gibbs Francesco Ricci Guodong Li Hong Zhu Kristin Persson Gerbrand Ceder Geoffroy Hautier Anubhav Jain G.Jeffrey Snyder | 2017 | npj Computational Materials2017，，1： | 11 |
| 19 | Nonunion of coronal shear fracture of femoral condyle显示文摘大腿骨的髁的孤立的花冠破裂在成年人是稀罕的并且 Hoffa 骨折不属于工会被报导仅仅在文学的一些时间。我们分析了六个案例在三年的一个时期上的 Hoffa 破裂不属于工会。三个病人保存地被对待,三个病人有固定失败。表示的延期是到一年的 2 个月。治疗协议与膝 arthrolysis 一起由开的减小, pseudoarthrosis 的切除,骨头 grafting 和内部固定组成了。联合在平均数 16 星期在所有病人被完成。治疗 Hoffa 破裂不属于工会要求小心的外科手术前的计划和过细的外科的技术。关于在破裂管理和外科的技术的争吵的文学被考察。 | Ajay Pal Singh Ish Kumar Dhammi Raju Vaishya Anil Kumar Jain Arun Pal Singh Prashant Modi | 2011 | Chinese Journal of Traumatology2011，14，3： | 11 |
| 20 | Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf显示文摘Objective:To evaluate the cytotoxicity and hepatoprotective potentials of extracts,fractions or isolated compound from the leaves of Feronia limonia(F.limonia).Methods:Qualitative phytochemical analysis of extracts,fractions or compound was performed by means of thin layer chromatography and spectroscopic assays.The%purity of compound was measured by analytical HPLC.Extracts,fractions or compound have been individually evaluated for their cytotoxicity effects(10,20,100,250,500,750 and 1 000 μg/mL).Based on the inhibitory concentration(IC_(50)) obtained from the cell viability assay,graded concentrations of extracts,fractions or isolated compound were assessed(10,20,50,100,200 μg/mL) for its hepatoprotective potential against CCl_4-induced hepatotoxicity by monitoring activity levels of serum glutamatic pyruvatic transaminase(SGPT) and serum glutamic oxaloacetic transaminase(SGOT).Results:Results indicated that the methanol extract of F.limonia was non-toxic and hepatoprotective in nature as compared with the petroleum ether extract.The acetone fraction of methanolic extract also showed similar properties but the subsequent two fractions were cytotoxic.However,the pure compound isolated from the penultimate fraction of methanolic extract was non-toxic and hepatoprotective in nature.Biochemical investigations(SCOT,SCPT) further corroborated these cytological observations.Conclusions:It can be concluded from this study that F.limonia methanol extract,some fractions and pure isolated compound herein exhibit hepatoprotective activity.However,cytotoxicity recorded in the penultimate fraction and investigation of structural details of pure compound warrants further study. | Mahendra Jain Rakhee Kapadia Ravirajsinh N Jadeja Menaka C Thounaojam Ranjitsinh V Devkar SH Mishra | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，6： | 11 |
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