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| 1 | 牛肺肝素寡糖的制备显示文摘利用肝素酶 (Heparinase I,EC4 .2 .2 .7)对牛肺肝素进行控制酶解 ,混合寡糖经超滤、凝胶渗透色谱和高压液相色谱技术分离制备后 ,得到聚合度为 2 ,4 ,6 ,8,10 ,12 ,14和 2 0的寡糖纯品。各寡糖纯度采用毛细管电泳和聚丙烯酰胺凝胶电泳进行检验 ,寡糖结构采用核磁共振氢谱证实。 | 于广利 王群 管华诗 徐家敏 Robert J.Linhardt | 2002 | 青岛海洋大学学报(自然科学版)2002，32，2： | 2 |
| 2 | Immobilization of Heparin:Approaches and Applications显示文摘 | Saravanababu Murugesan Jin Xie Robert J.Linhardt | | 0，，02： | 1 |
| 3 | 硫酸皮肤素寡糖的分离与制备显示文摘利用软骨素酶 ABC (Chondroitinase ABC,EC4 .2 .2 .4 )对硫酸皮肤素 (Dermatan Sul-fate,DS)进行控制降解。对得到的混合寡糖首先采用低压凝胶渗透色谱 (L PGPC)进行分级 ,然后对每一组分再利用强阴离子交换高压液相色谱 (SAX- HPL C)进行分离 ,最终制备出聚合度为 2 ,4 ,6 ,8,10 ,12的寡糖纯品。寡糖纯度采用 SAX- HPL C,毛细管电泳 (CE)以及聚丙烯酰胺凝胶电泳(PAGE)进行检验 ,结构采用电喷雾离子化质谱 (ESI- MS)以及核磁共振波谱 (NMR) | 于广利 管华诗 Robert J.Linhardt Wang Qun Hyun OK Yang Toshihiko Toida | 2001 | 青岛海洋大学学报(自然科学版)2001，31，6： | 1 |
| 4 | 肝素酶Ⅰ对合成的肝素五糖的酶解作用及对其抗Xa因子活性的影响(英文)显示文摘研究肝素酶 (来源于 Flavobacterum heparinase,EC4 .2 .2 .7)对人工合成的且含有与抗凝血酶 特定结合位点的肝素五糖 (SHP)的酶解作用 ,并对酶解作用的动力学进行研究。利用强阴离子高效液相色谱 (SAX- HPL C)对酶解混合物进行分离 ,利用质谱 (ESIMS)和核磁共振波谱 (1H- NMR)技术对得到的二糖和三糖的结构进行确证。研究结果表明 ,这种被作为肝素反向试剂的肝素酶 可水解人工所合成的肝素五糖 ,从而使之丧失抗 Xa因子活性。 | 于广利 管华诗 Robert J.Linhardt Jawed Fareed | 2001 | 青岛海洋大学学报(自然科学版)2001，31，4： | 1 |
| 5 | E. coli K5 fermentation and the preparation of heparosan, a bioengineered heparin precursor显示文摘 | ZhenyuWang MellisaLy FumingZhang WeihongZhong AmySuen Anne MarieHickey Jonathan S.Dordick Robert J.Linhardt | 2010 | Biotechnol Bioeng2010，，6： | 1 |
| 6 | Unnatural Polyketide Analogues Selectively Target the HER Signaling Pathway in Human Breast Cancer Cells显示文摘 | Seok JoonKwon Moon IlKim BosungKu LydieCoulombel Jin‐HwanKim Joseph H.Shawky Robert J.Linhardt Jonathan S.Dordick | 2010 | Chem. Eur. J. of Chem. Bio2010，，4： | 1 |
| 7 | Bioengineered production of glycosaminoglycans and their analogues显示文摘Glycosaminoglycans(GAGs)are a class of linear polysaccharides,consisting of alternating disaccharide sequences of uronic acid and hexosamines(or galactose)with and without sulfation.They can interact with various proteins,such as growth factors,receptors and cell adhesion molecules,endowing these with various biological and pharmacological activi-ties.Such activities make GAGs useful in health care products and medicines.Currently,all GAGs,with the exception of hyaluronan,are produced by extraction from animal tissues.However,limited availability,poor control of animal tissues,impurities,viruses,prions,endotoxins,contamination and other problems have increased the interest in new approaches for GAG production.These new approaches include GAGs production by chemical synthesis,chemoenzymatic synthesis and metabolic engineering.One chemically synthesized heparin pentasaccharide,fondaparinux sodium,is in clinical use.Mostly,hyaluronan today is prepared by microbial fermentation,largely replacing hyaluronan from rooster comb.The recent gram scale chemoenzymatic synthesis of a heparin dodecasaccharide suggests its potential to replace currently used animal-sourced low molecular weight heparin(LMWH).Despite these considerable successes,such high-tech approaches still cannot meet worldwide demands for GAGs.This review gives a brief introduction on the manufacturing of unfractionated and low molecular weight heparins,the chemical synthesis and chemoenzymatic synthesis of GAGs and focuses on the progress in the bioengineered preparation of GAGs,particularly heparin. | Weihua Jin Fuming Zhang Robert J.Linhardt | 2021 | Systems Microbiology and Biomanufacturing2021，1，2： | 0 |
| 8 | N-glycans released from glycoproteins using a commercial kit and comprehensively analyzed with a hypothetical database显示文摘The glycosylation of proteins is responsible for their structural and functional roles in many cellular activities.This work describes a strategy that combines an efficient release, labeling and liquid chromatography–mass spectral analysis with the use of a comprehensive database to analyze N-glycans. The analytical method described relies on a recently commercialized kit in which quick deglycosylation is followed by rapid labeling and cleanup of labeled glycans. This greatly improves the separation, mass spectrometry(MS) analysis and fluorescence detection of N-glycans. A hypothetical database, constructed using Glyc Resoft, provides all compositional possibilities of N-glycans based on the common sugar residues found in N-glycans. In the initial version this database contains > 8,700 N-glycans, and is compatible with MS instrument software and expandable. N-glycans from four different well-studied glycoproteins were analyzed by this strategy. The results provided much more accurate and comprehensive data than that had been previously reported. This strategy was then used to analyze the N-glycans present on the membrane glycoproteins of gastric carcinoma cells with different degrees of differentiation. Accurate and comprehensive N-glycan data from those cells was obtained efficiently and their differences were compared corresponding to their differentiation states. Thus, the novel strategy developed greatly improves accuracy, efficiency and comprehensiveness of N-glycan analysis. | Xue Sun Lei Tao Lin Yi Yilan Ouyang Naiyu Xu Duxin Li Robert J.Linhardt Zhenqing Zhang | 2017 | Journal of Pharmaceutical Analysis2017，7，2： | 0 |
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