| 3 | 类SCC样甲氧西林敏感性金黄色葡萄球菌的基因组学分析显示文摘金黄色葡萄球菌(Staphylococcus aureus,S.aureus),作为一种常见的致病菌常可导致严重感染性疾病.金黄色葡萄球菌的主要特点是易产生耐药性.临床上运用多重PCR方法对S.aureus耐药菌株进行分型.SCC作为一种mecA基因的转运载体,介导金黄色葡萄球菌产生耐药性.本研究报道了一株新甲氧西林敏感性金黄色葡萄球菌的全基因组序列信息,即类SCC样MSSA463菌株,经多重PCR方法,该菌株被误鉴定为耐甲氧西林金黄色葡萄球菌,结果与药敏结果相冲突.为此,运用焦磷酸测序方法完成了该菌株的全基因组测序,并与已知金黄色葡萄球菌的基因组序列信息不同,发现可读框(CZ049;AB037671)存在于attL,attR反向重复序列的临近下游.这些结果提示,在金黄色葡萄球菌与其他微生物之间可能存在一种水平基因转移,并改变了金黄色葡萄球菌对药物的敏感性,推测attL,attR反向重复序列可能作为外源性基因的插入部位而存在. | 李德志 楚亚男 任鲁风 李欣刚 袁丽娜 康禹 张伟 杨宇 王绪敏 Baillie J.Kenneth 于军 高占成 | 2013 | 中国科学:生命科学2013，43，3： | 0 |
| 4 | Complete genome sequence of methicillin-sensitive Staphylococcus aureus containing a heterogeneic staphylococcal cassette chromosome element显示文摘Staphylococcus aureus is a common human bacterium that sometimes becomes pathogenic,causing serious infections.A key feature of S.aureus is its ability to acquire resistance to antibiotics.The presence of the staphylococcal cassette chromosome(SCC) element in serotypes of S.aureus has been confirmed using multiplex PCR assays.The SCC element is the only vector known to carry the mecA gene,which encodes methicillin resistance in S.aureus infections.Here,we report the genome sequence of a novel methicillin-sensitive S.aureus(MSSA) strain:SCC-like MSSA463.This strain was originally erroneously serotyped as methicillin-resistant S.aureus in a clinical laboratory using multiplex PCR methods.We sequenced the genome of SCC-like MSSA463 using pyrosequencing techniques and compared it with known genome sequences of other S.aureus isolates.An open reading frame(CZ049;AB037671) was identified downstream of attL and attR inverted repeat sequences.Our results suggest that a lateral gene transfer occurred between S.aureus and other organisms,partially changing S.aureus infectivity.We propose that attL and attR inverted repeats in S.aureus serve as frequent insertion sites for exogenous genes. | LI DeZhi CHU YaNan REN LuFeng LI XinGang YUAN Lina KANG Yu ZHANG Wei YANG Yu WANG XuMin BAILLIE J.Kenneth YU Jun GAO ZhanCheng | 2013 | Science China(Life Sciences)2013，56，3： | 0 |
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