| 7 | The Apoptosis and CellCycle Changes of SUD4 and DOHH2 Induced by Topotecan显示文摘Topotecan (TPT), a semisynthetic analogue of the natural product camptothecin is a cell cyciespeclrlc drug with antitumor activity. To clarify the effect of TPT on SUD4 and DOHH2 cell line in this study, we examined the apoptosis and cell cycle changes or the two human cancer cell lines by exposing to TPT for 18 hours at various concentrations. The linear relationship between apoptosis cell number and the concentration of TPT was observed by means of Flow Cytometry and Annexin V assay. Then, DOHH2 cell is much more sensitive to TPT than SUD4 cell. In addition, Cell Question Software Assay showed positive relationship between the frequency of cells accumulated in S-phase and the concentration of TPT. The least concentration of TPT to change cell cycle is 5 nmol’ L-1 in both cell lines. These results suggest that the inducing apoptosis of cancer cells is one of mechanism of TPT antitumor activity. | Feng Xiaorong Zheng Junying Deng Fengjiao Paul Smith Cat Kun(Unit of Molecular Biology, Israel National Institute of Oceanography,Tel Shikmona, P. O. B. 8030, HAIFA 31080, ISRAELA)(College of Life Sciences, Wuhan University, Wuhan 430072, China)(Departmen | 1998 | Wuhan University Journal of Natural Sciences1998，3，4： | 0 |
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