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| 1 | Juvenile ferric iron prevents microbiota dysbiosis and colitis in adult rodents显示文摘AIM: To assess whether juvenile chronic ferric iron ingestion limit colitis and dysbiosis at adulthood in rats and mice. METHODS: Two sets of experiments were designed. In the first set, recently weaned mice were either orally administered ferrous (Fe2+) iron salt or ferric (Fe3+) microencapsulated iron for 6 wk. The last week of experiments trinitrobenzene sulfonic acid (TNBS) colitis was induced. In the second set, juvenile rats received the microencapsulated ferric iron for 6 wk and were also submitted to TNBS colitis during the last week of experiments. In both sets of experiments, animals were sacrificed 7 d after TNBS instillation. Severity of the inflammation was assessed by scoring macroscopic lesions and quantifying colonic myeloperoxidase (MPO) activity. Alteration of the microflora profile was estimated usingquantitative polymerase chain reaction (qPCR) by measuring the evolution of total caecal microflora, Bacteroidetes, Firmicutes and enterobacteria. RESULTS: Neither ferrous nor ferric iron daily exposures at the juvenile period result in any effect in control animals at adulthood although ferrous iron repeated administration in infancy limited weight gain. Ferrous iron was unable to limit the experimental colitis (1.71 ± 0.27 MPO U/mg proteinvs 2.47 ± 0.22 MPO U/mg protein in colitic mice). In contrast, ferric iron significantly prevented the increase of MPO activity (1.64 ± 0.14 MPO U/mg protein) in TNBS-induced colitis. Moreover, this positive effect was observed at both the doses of ferric iron used (75 and 150 mg/kg per day po - 6 wk). In the study we also compared, in both rats and mice, the consequences of chronic repeated low level exposure to ferric iron (75 mg/kg per day po - 6 wk) on TNBS-induced colitis and its related dysbiosis. We confirmed that ferric iron limited the TNBS-induced increase of MPO activity in both the rodent species. Furthermore, we assessed the ferric iron incidence on TNBS-induced intestinal microbiota dysbiosis. At first, we needed to optimize the isolation and quantify DNA copy numbers using standard curves to perform by qPCR this interspecies comparison. Using this approach, we determined that total microflora was similar in control rats and mice and was mainly composed of Firmicutes and Bacteroidetes at a ratio of 10/1. Ferric juvenile administration did not modify the microflora profile in control animals. Total microflora numbers remained unchanged whichever experimental conditions studied. Following TNBS-induced colitis, the Firmicutes/Bacteroidetes ratio was altered resulting in a decrease of the Firmicutes numbers and an increase of the Bacteroidetes numbers typical of a gut inflammatory reaction. In parallel, the subdominant population, the enterobacteria was also increased. However, ferric iron supplementation for the juvenile period prevented the increase of Bacteroidetes and of enterobacteria numbers consecutive to the colitis in both the studied species at adulthood.CONCLUSION: Rats and mice juvenile chronic ferric iron ingestion prevents colitis and dysbiosis at adulthood as assessed by the first interspecies comparison. | Chourouk Ettreiki Pascale Gadonna-Widehem Irène Mangin Mose Coёffier Carine Delayre-Orthez Pauline M Anton | 2012 | World Journal of Gastroenterology2012，18，21： | 3 |
| 2 | Management of venous thromboembolism in patients with advanced cancer: a systematic review and meta-analysis显示文摘 | Simon IR Noble Mike D Shelley Bernadette Coles Susan M Williams Andrew Wilcock Miriam J Johnson | 2008 | Lancet Oncology2008，，6： | 2 |
| 3 | Oxidative stress triggers cardiac fibrosis in the heart of diabetic rats显示文摘 | Aragno M Mastrocola IR Alloatti G | 2008 | Endocrinology2008，149，1： | 1 |
| 4 | Position-specificity of facial adaptation显示文摘 | Gyula Kovács Márta Zimmer Irén Harza Andrea Antal Zoltán Vidnyánszky | 2005 | NeuroReport2005，，17： | 1 |
| 5 | Cutting edge:TREM-2 attenuates macrophage activation显示文摘 | Tumbull IR Gilfillan S Cella M | 2006 | J Immunol2006，177，6： | 1 |
| 6 | Risks of non -sedating antihistamines 显示文摘 | Lindquist M Edward IR | 1997 | Lancet1997，349，9061： | 1 |
| 7 | Does sufentanil concen- tration influence isoflurane requirements during coronary artery bypass grafting显示文摘 | Thomson IR Moon M Hudson RJ | 1999 | J Cardiotborac Vasc Anesth1999，13，1： | 1 |
| 8 | Type Ⅴ protein secretion pathway:the autotransporter story显示文摘 | Henderson IR Navarro-Garcia F Desvaux M | 2004 | Microbiol Mol Biol Rev2004，68，： | 1 |
| 9 | A data mining approach for signal detection and analysis显示文摘 | Bate A Lindquist M Edward IR | 2002 | Drug saf2002，25，6： | 1 |
| 10 | Fold and function of polypeptide transport-associated domains responsible for delivering unfolded proteins to membranes显示文摘 | Knowles TJ Jeeves M Bobat S Dancea F McClelland D Palmer T Overduin M Henderson IR | | 0，，05： | 1 |
| 11 | Localization of the extracellular matrix protein SC1 to synapses in the adult rat brain 显示文摘 | Lively S Ringuette M J Brown IR | 2007 | Neurochem Res2007，32，1： | 1 |
| 12 | Adrenal mas- ses: characterization with combined unenhanced and de- layed enhanced CT显示文摘 | Caoili EM Korobkin M Francis IR | 2002 | Radiology2002，222，3： | 1 |
| 13 | Risk of nonsedating antihistamine显示文摘 | Linduist M Edwars IR | 1997 | Lancet1997，349，9061： | 1 |
| 14 | Molecular evolution and balancing selection in the flavin-containing monooxygenase 3 gene (FMO3)显示文摘 | Allerston CK Shimizu M Fujieda M Shephard EA Yamazaki H Phillips IR | 2007 | Pharmacogenet Genomics2007，17，10： | 1 |
| 15 | Effect of age at on- set of schizophrenia on white matter abnormalities显示文摘 | Kyriakopoulos M Perez IR Woolly JB | 2009 | Br J Psy- chiatry2009，195，4： | 1 |
| 16 | Type Ⅴ protein secretion pathway:the autotransporter story显示文摘 | Henderson IR Navarro-Garcia F Desvaux M | | 0，，04： | 1 |
| 17 | Demyelination during multiple sclerosis is associated with combined activation of microglia/macrophages by IFN-y and alpha B-crystallin显示文摘 | Bsibsi M Peferoen LA Holtman IR | 2014 | Acta Neuropathol2014，128，2： | 1 |
| 18 | Delayed enhanced CT for differentiation of benign from malignant adrenal masses显示文摘 | Korobkin M Brodeur FJ Francis IR | 1996 | Radiology1996，200，3： | 1 |
| 19 | Glyp ican23 : a novelserum and histochemical marker for hepatocellular carcinoma 显示文摘 | CapurroM Wanless IR Sherman M | 2003 | Gastroenterology2003，125，： | 1 |
| 20 | Estrogen-induced activation of mitogen-activated protein kinase in cerebral Cortical explants convengence of estrogen and neurotrophin signaling pathways显示文摘 | Singh M Ir Gs Guan XP | 1999 | Indothology1999，19，： | 1 |
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