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| 1 | 下腔静脉滤器置入术和取出术规范的专家共识显示文摘在回顾历史、分析现状的基础上,提出下腔静脉滤器置入术和取出术的适应证与禁忌证。对围手术期处理、操作步骤、并发症防治作了归纳总结和说明。强调尽量使用临时性滤器和可取出滤器,以降低由于滤器长期置入而引起的并发症的发生率。 | Academic Group of Interventional Radiology,Radiology Branch of Chinese Medical Association.Department of Radiology,the First Hospital Affiliated to Nanjing Medical University,Nanjing 210006,China | 2011 | 介入放射学杂志2011，20，5： | 51 |
| 2 | 经颈静脉肝内门体静脉分流术治疗肝硬化门静脉高压共识意见显示文摘门静脉高压症是肝硬化发展过程中的重要病理生理环节,也是肝硬化失代偿期的重要临床表现之一。经颈静脉肝内门体静脉分流术(transjugular intrahepatic portosystemic shunt,TIPS)通过在肝静脉与门静脉之间的肝实质内建立分流道,以微创的方式,从结构上显著降低门静脉阻力,是降低肝硬化患者门静脉压力的关键措施之一。选择恰当病例,可有效减少食管胃静脉曲张再出血和腹水复发等肝硬化并发症,改善肝硬化患者生活质量,减少或延缓对肝移植的需求^[1-3]。 | Group of Gastrointestinal Intervention,Chinese Society of Gastroenterology,Chinese Medical Association | 2014 | 临床肝胆病杂志2014，30，3： | 58 |
| 3 | 早期干预促进早产儿智能发育显示文摘目的改善早产儿的预后,进行早期干预的协作研究。方法将103例早产儿随机分为干预组(52例)和常规育儿组(51例)。正常对照组(53例)。干预组从新生儿期开始接受智能早期干预,早产儿两组的围产期情况相似。结果纠正年龄1岁半、2岁时干预组精神发育指数(MDI)比常规育儿组平均高138和146(P<0.001),达正常组水平(P>0.05)。而常规育儿组MDI比正常对照组分别低115分和89分(P<0.05)。其中智力低下4例(7.8%)(4/51),干预组中无一例智力低下。结论早期干预可以促进早产儿智能发育,有利于防治其智力低下。 | Early Intervention of Premature Infant Coorperative Research Group, Beijing 100730 | 1998 | 中华儿科杂志1998，36，10： | 47 |
| 4 | 全国第二次冠心病介入性治疗病例注册登记资料分析显示文摘目的 分析 1997年和 1998年我国冠心病介入性治疗发展趋势。方法 采用填写统一发放的表格的方法 ,收集 1997、1998两年各医院冠心病介入性治疗病例资料 ,进行回顾性分析。结果共注册经皮冠状动脉腔内成形术 (PTCA)病例总数 872 5例 ,来自全国范围内 75所医院 ,总成功数 83 69例 (95 9% )。其中 1997年 3 74 0例 ,成功 3 5 5 3例 (95 0 % ) ;1998年 4 985例 ,成功 4 816例 (96 6% )。共扩张病变 10 2 18处 ,成功 965 0处 ,病变成功率为 94 4 %。急性心肌梗死 (AMI)急诊PTCA 10 4 5例 ,成功10 0 1例 (95 8% )。全组患者发生重要并发症 95 9例 (11 0 % ) ,包括死亡 3 2例 (0 3 7% )。冠状动脉支架置入术 63 2 9例 ,共置入支架 774 8只 ,其中左冠状动脉主干置入支架 86只 (占 1 1% )。置入成功7677只 (99 1% )。发生重要并发症 5 10例 (8 1% ) ,包括死亡 2 5例 (0 4 0 % )。全部患者服用噻氯匹啶 ,其中 13 8例 (1 8% )发生粒细胞减少症。另外 ,注册登记冠状动脉斑块旋磨术 93例 (94支血管 ) ,成功率为 93 6%。结论 1997年和 1998年我国冠心病介入治疗例数有大幅度增长 ,两年内完成病例数远远超过过去 12年积累病例总数 ,成功率较过去有增加趋势 ,但地区间及医院间发展仍很不平衡。 | Section of Interventional Cardiology, Chinese Society of Cardiology, Chinese Medical Association, et al. (Correspondent: GAO Runlin. Fu Wai Hospital, PUMC & CAMS, Beijing 100037, China) | 2000 | 中华心血管病杂志2000，28，1： | 50 |
| 5 | Treatment of large lumbar disc herniation with percutaneous ozone injection via the posterior-lateral route and inner margin of the facet joint显示文摘AIM:To evaluate the effects of percutaneous ozone injection via the posterior-lateral route and inner margin of the facet joint in the treatment of large lumbar disc herniation.METHODS:Fifty-eight patients with large lumbar disc herniation were treated with percutaneous injection of ozone via the posterior-lateral route and inner margin of the facet joint under digital subtraction angiography.Second injections were performed 5 d after the initial injection.All patients were followed up for 6-18 mo.A modified Macnab method was used for assessing clinical outcomes after oxygen-ozone therapy.RESULTS:Successful puncture was obtained in all patients.The overall efficacy was 91.4;the outcome was the excellent in 37 cases(63.8),good in 16 cases(27.6) and fair/poor in 5 cases(8.6) according to the Macnab criteria.No severe complications were found throughout this study.CONCLUSION:Percutaneous intradiscal ozone injection via the posterior-lateral route and inner margin of the facet joint is effective and safe for treatment of large lumbar disc herniation. | Wei Lu,Yan-Hao Li,Xiao-Feng He,Department of Interventional Radiology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,Guangdong Province,China | 2010 | World Journal of Radiology2010，2，3： | 31 |
| 6 | Mechanism of exosomal microRNA-224 in development of hepatocellular carcinoma and its diagnostic and prognostic value显示文摘BACKGROUND Exosomes contain proteins, lipids, and biological molecules such as DNA and RNA. Nucleic acids in exosomes are a group of molecules that can act as biomarkers. Currently, there are many reports on exosomal microRNAs, which are ideal biomarkers for the early diagnosis of cancer. However, there are few reports on the role of exosomal microRNAs in the diagnosis and prognosis of hepatocellular carcinoma(HCC).AIM To understand the mechanism of exosomal microRNA-224(miR-224) in the development of HCC and evaluate its diagnostic and prognostic value.METHODS Cell culture and transfection of exosomal miRNA-224, real-time quantitative PCR, luciferase reporter assay, and other methods were used to find new biomarkers related to the development of HCC that can be used to diagnose HCC and predict HCC prognosis.RESULTSBy targeting glycine N-methyltransferase, incubating exosomes with miR-224 mimic resulted in a significant increase in cell proliferation compared to that of the control group, while incubation with the miR-224 inhibitor significantly reduced cell proliferation. The same results were obtained for the cell invasion assay. Serum exosomal miR-224 did have some ability to differentiate patients with HCC from healthy controls, with an area under the curve of 0.910, and HCC patients with higher serum exosomal miR-224 expression had lower overall survival.CONCLUSION Exosomal miR-224 is a tumor promotor and can be a marker of diagnosis and prognosis of HCC patients, however, its ability to distinguish liver diseases needs further verification. | Yao Cui Hai-Feng Xu Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Interventional Therapy Department Peking University Cancer Hospital and Institute Ming-Yue Liu Yu-Jie Xu Jun-Chuang He Yun Zhou Shun-Dong Cang | 2019 | World Journal of Gastroenterology2019，25，15： | 26 |
| 7 | An implantable rat liver tumor model for experimental transarterial chemoembolization therapy and its imaging features显示文摘AIM: To establish an ideal implantable rat liver tumor model for interventional therapy study and examine its angiographic signs and MRI, CT features before and after embolization. METHODS: Forty male Wistar rats were implanted with Walker256 tumor in the left lateral lobe of liver. Digital subtraction angiography (DSA) and transarterial chemoembolization were performed on day 14 after implantation. Native computer tomography (CT, n=8) and native magnetic resonance (MR,n=40) were performed between the day 8 and day 21 after implantation. The radiological morphological characteristics were correlated with histological findings.RESULTS: Successful implantation was achieved in all forty rats, which was confirmed by CT and MRI. MR allowed tumor visualization from day 8 while CT from day 11 after implantation. The tumors were hypodensity on CT, hypointense on MR T1-weighted and hyperintense on T2-weighted. The model closely resembled human hepatocardnoma in growth pattem and the lesions were rich in vasculature on angiography and got its filling mainly from the hepatic artery. Before therapy, tumor size was 211.9±48.7 mm3. No ascites, satellite liver nodules or lung metastasis were found. One week after therapy, tumor size was 963.6±214.8 mm3 in the control group and 356.5±78.4mm3 in TACE group. Ascites (4/40), satellite liver nodules (7/40) or lung metastasis (3/40) could be seen on day 21.CONCLUSION: Walker-256 tumor rat model is suitable for the interventional experiment. CT and MRI are helpful in animal optioning and evaluating experimental results. | Xin Li Chuan-Sheng Zheng Gan-Sheng Feng Chen-Kai Zhuo Jun-Gong Zhao Xi Liu,Department of Interventional Radiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China | 2002 | World Journal of Gastroenterology2002，8，6： | 20 |
| 8 | Influence of transarterial chemoembolization on angiogenesis and expression of vascular endothelial growth factor and basic fibroblast growth factor in rat with Walker-256 transplanted hepatoma:An experimental study显示文摘AIM: After transarterial chemoembolization (TACE), the residual cancer cells are under extensive hypoxic or even anoxic environment. Hypoxia can lead to adaptive responses.For example, angiogenesis will help these cells survive. In this study, we examined the effect of TACE on angiogenesis and expression of vascular endothelial growth factor (VEGF)and basic fibroblast growth factor (b-FGF) and to assess their relevance to Walker-256 transplanted hepatoma.METHODS: Male Wistar rats were inoculated with Walker256 tumor in the left lobe of liver. Angiography and transarterial chemoembolization were performed at d14 after transplantation. Sixty rats bearing walker-256 transplanted hepatoma were randomly divided into control group, arterial infusion group and TACE group. Each group consisted of twenty rats. Normal saline, 5-Fu, 5-Fu and lipiodol were infused through hepatic artery respectively. Two weeks after the infusion, staining of factor Ⅷ, VEGF and b-FGF was performed by immunohistochemistry method in routine paraffin-embeded sections. Microvessel density(MVD) was counted in endothelial cells with positive factor Ⅷ. Their expression levels were analyzed in conjunction with the pathologic features.RESULTS: While a smaller tumor volume was found in TACE group (F=37.818, P<0.001), no statistical differences between MVD and expression of VEGF and b-FGF were found among the 3 groups. MVD of the control group, chemotherapy group and chemoemoblization group was 80.84±24.24,83.05±20.29 and 85.20±23.91 (F=0.193, P=0.873),respectively. The positive expression of VEGF and b-FGF was 75 %, 75 % , 85 % (X2=0.449, P=0.799) and 30 %,25 %, 30 % (X2=0.141, P=0.922), respectively. Statistical analysis revealed a positive correlation between the expression of VEGF and MVD (r=0.552, P<0.001).CONCLUSION: There has been little influence of lipiodol chemoembolization on the formation of tumor angiogenesis,but the development of neovascularization and expressionof VEGF play important roles in establishment of collateralcirculation and reconstruction of blood supply of residualcancer tissue. | Xin Li Gan-Sheng Feng Chuan-Sheng Zheng Chen-Kai Zhuo Xi Liu, Department of Interventional Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China | 2003 | World Journal of Gastroenterology2003，9，11： | 20 |
| 9 | Necessity and indications of invasive treatment for Budd-Chiari syndrome显示文摘BACKGROUND:The development of collaterals in Budd-Chiari syndrome has been described and these collaterals play an important role in the presentation of this disease.These collaterals are diagnostic and their use in management strategy has never been evaluated.This study aimed to investigate the indications,feasibility and necessity of invasive treatment for patients with Budd-Chiari syndrome and to determine whether such a strategy is necessary for optimal management.METHODS:Twenty-nine patients who had been treated at our unit were enrolled in this study.Based on physical and biochemical examination,and hemodynamic compensation by collaterals,18 patients underwent radiological intervention (group A),while the other 11 had no invasive treatment (group B).The related hemodynamic parameters were acquired when percutaneous angiography was performed.RESULTS:In group A,all patients underwent successfully inferior vena cava (IVC) balloon angioplasty with or without stenting.Four patients also underwent hepatic vein angioplasty.In these patients,the mean IVC pressure before and after treatment was statistically different (29.3±9.2 vs 15.1±4.6 mmHg,P<0.01).The mean IVC pressure was much lower in group B than in group A (12.9±2.4 vs 29.3±9.2 mmHg,P<0.01),but there was no difference from that of the patients after radiological treatment (12.9±2.4 vs 15.1±4.6 mmHg,P>0.05).Median follow-up was 32.3 months (mean 21.3 months;range 3-61 months).In the course of follow-up,the patients in group A survived with good systemic status except for re-stenosis in one patient who underwent re-canalization of the IVC.In group B,10 patients had good systemic status except one patient who had a meso-caval shunt because of deterioration.CONCLUSIONS:The rationale of 'early diagnosis and early treatment' is not suitable for all patients with Budd-Chiari syndrome.Satisfactory survival can be achieved in some patients without invasive treatment,who are completely compensated by rich collaterals.Nonetheless,a positive treatment procedure should be performed if the patient's situation worsens in the course of regular follow-up. | Department of General Surgery (Fu Y,Sun YL,Ma XX,Xu PQ,Feng LS,Tang Z and Luo CH),Institute of Hepatic Vascular Disease (Sun YL),Department of Radiological Intervention (Guan S and Wang ZW),First Affiliated Hospital,Zhengzhou University School of Medicine,Zhengzhou 450052,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 10 |
| 10 | Transarterial embolization ablation of hepatocellular carcinoma with a lipiodol-ethanol mixture显示文摘AIM: To determine the safety and effectiveness of transarterial embolization ablation (TEA) of hepatocellular carcinoma (HCC) with a lipiodol-ethanol mixture. METHODS: Between January 1 and December 31, 2009, 15 patients with HCC (13 men/two women, aged 38-75 years) accepted TEA treatment and were enrolled in this study, including five newly diagnosed patients and 10 with refractory disease. Two months after TEA, angiography and contrast computed tomography (CT) were performed, and responses were assessed using a modified version of Response Evaluation Criteria in Solid Tumors (RECIST version 1.1). The follow-up period was to June 30, 2010. RESULTS: Every new case was treated once. Angiography was performed immediately after TEA, and showed that the tumor-feeding vessels were completely embolized and that lipiodol was densely deposited inside tumors. Two months after treatment, contrast CT showed no enhanced lesions. Alpha fetoprotein levels returned to normal in four patients and markedly decreased in another. mean ± SD survival after treatment was 10.8 ± 4.5 mo. All five patients survived during the follow-up period. Ten patients with refractory disease were treated a total of 14 times. Angiography immediately after TEA showed that blood flow to the tumors was obviously decreased in all cases, and contrast CT showed obvious depositions of lipiodol. Two months after treatment, the tumors had shrunk (6/10) or were stable (3/10). One had progressed after 2 mo and died of tumor rupture 3 mo after TEA. mean ± SD survival after treatment was 8.6 ± 4.3 mo; two patients survived during the follow-up period. Adverse effects included reversible hepatic decompensation, upper abdominal pain, and fever. CONCLUSION: TEA is an effective therapy for patients with HCC and might be more effective than transcather arterial chemoembolization for treating refractory disease. | Yang-Kui Gu, Rong-Guang Luo, Jin-Hua Huang, Qi-Jiao Si Tu, Xiao-Xia Li, Fei Gao,State Key Laboratory of Oncology in Southern China,Department of Medical Imaging and Interventional Radiology,Cancer Center,Sun Yat-sen University,Guangzhou510060,Guangdong Province,China Department of Medical Imaging and Interventional Radiology, the First Affiliated Hospital of Nan-Chang University, 17 Yongwaizheng Street, Nanchang 330006, Jiangxi Province, China | 2010 | World Journal of Gastroenterology2010，16，45： | 9 |
| 11 | Clinical practice of transarterial chemoembolization for hepatocellular carcinoma:consensus statement from an international expert panel of International Society of Multidisciplinary Interventional Oncology(ISMIO)显示文摘Importance:Transarterial chemoembolization(TACE)has been associated with a wide range of practice variations for hepatocellular carcinoma(HCC)between the East and the West.This considerable ambiguity may lead to the heterogeneous quality in treatment and have a negative impact on the role of TACE in the overall multidisciplinary HCC treatment system.Objective:It may be a good start to establish a guideline worldwide to have this consensus from experts who represent east and west,although it does not cover all aspects of TACE.Evidence Review:An international expert panel on TACE is convened to cluster the expert’s opinions and summary a standard consensus.This panel committee consist of leading physicians in TACE on HCC from USA,France,Japan,Singapore,Korea,China,and so on.The first-round face-to-face consensus meeting was held during in Nanjing,China in October 2019.The second-round conference for revision of the consensus was held during the Annual Meeting of Chinese College of Interventionalists in August 2020 by a hybrid format of a Webinar and roundtable meeting.After several on-line revisions,the final manuscript was approved by all members of the panel in June 2021.Findings:The consensus statements were organized into the following categories:patients’selection,performing the procedure,TACE outcomes,repeat TACE,TACE failure/refractory,and TACE-based combination treatments.Conclusions and Relevance for Reviews:More and more evidences have showed the better outcomes with strategy of combined TACE with other local therapies such as ablations.The most-recently developing strategy of combined TACE with PD-1/PD-L1 plus tyrosine kinase inhibitor(TKI)agents has shined a light to the HCC patients,especially to those with high risk of tumor recurrence after treatment or TACE failure/refractory. | Jian Lu Ming Zhao Yasuaki Arai Bin-Yan Zhong Hai-Dong Zhu Xiao-Long Qi Thierry de Baere Uei Pua Hyun Ki Yoon David C.Madoff Gao-Jun Teng International Society of Multidisciplinary Interventional Oncology(ISMIO) | 2021 | Hepatobiliary Surgery and Nutrition2021，10，5： | 9 |
| 12 | Percutaneous transhepatic metal versus plastic biliary stent in treating malignant biliary obstruction: a multiple center investigation显示文摘OBJECTIVE: To compare the efficacy of metal versus plastic biliary stent implantation in the treatmentof malignant biliary obstruction.METHODS: Percutaneous transhepatic self-expandable metal stent (MS, n=61) and 10F plastic stent(PS, n=34) were placed in 95 patients with malignant biliary obstruction in three hospitals of Guangdongprovince. All patients were followed up until death or at least one year after the procedure. Kaplan-Meieranalysis was used to compare the survival of the patients and the rates of stent patency.RESULTS: The 30-day mortality rate was lower in the MS group (6/61, 9.8%) than in the PS group(9/34, 26.5%, P<0.05). The 30-day reobstruction rate and the complication rate were 15.0%, 16.4%in the MS group and 32. 4%, 29. 4% in the PS group, respectively (P<0. 01). The median patencyperiod of stents and median survival period of the patients were 230 days, 224 days in the MS group and90 days, 94 days in the PS group, respectively (P<0. 01).CONCLUSION: Metal stent is clinically superior to plastic stent in the treatment of malignant biliaryobstruction. | Yuan-Xing Guo Yan-Hao Li Yong Chen Ping-Yan Chen Peng-Fei Luo Yong Li Hong Shan Zai-Bo Jiang the Department of Interventional Radiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China Department of Medical Statistics, First Military Medical University, Guangzhou 510515, China Department of Radiology, Guangdong Provincial People’s Hospital, Guangzhou 510080, China Department of Radiology, Third Affiliated Hospital of Sun Yat-Sen University of Medical Sciences, Guangzhou 510630, China | 2003 | Hepatobiliary & Pancreatic Diseases International2003，2，4： | 8 |
| 13 | Treatment of malignant digestive tract obstruction by combined intraluminal stent installation and intra-arterial drug infusion显示文摘AIM To study the palliative treatment of malignant obstrution of digestive tract with placement of intraluminal stent combined with intra-arterial infusion of chemotherapeutic drugs.METHODS A total of 281 cases of digestive tract malignant obstruction were given per oral (esophagus,stomach, duodenum and jejunum), per anal (colon and rectum ) and percutaneous transhepatic ( biliary )installation of metallic stent. Among them, 205 cases received drug infusion by cannulation of tumor supplying artery with Seldingers technique.RESULTS Altogether 350 stents were installed in 281 cases, obstructive symptoms were relieved or ameliorated after installation. Occurrence of restenotic obstruction was 8 - 43 weeks among those with intraarterial drug infusion, which was later than 4 - 26 weeks in the group with only stent installation. The average survival time of the former group was 43 (3 - 105) weeks,which was significantly longer than 15 (3- 24) weeks of the latter group.CONCLUSION Intraluminal placement of stent combined with intra-arterial infusion chemotherapy is one of the effective palliative therapies for malignant obstruction of the digestive tract with symptomatic as well as etiological treatment. | Ai-Wu Mao Zhong-Du Gao Jia-Yu Xu Ren-Jie Yang Xiang-Seng Xiao Ting-Hui Jiang Wei-Jun Jiang Department of Interventional Radiology,Shanghai S.T,Luke’s Hospital,768 Yu Yuan Road,Shanghai 200050,ChinaDepartment of Gastroenterology Rui Jin Hospital,Shanghai Second Medical University,Departrnent of Tumor Hospital,Beijing Medical UniversityDepartment of Imaging Chang Zheng Hospital,Shanghai Second Military Medical University | 2001 | World Journal of Gastroenterology2001，7，4： | 6 |
| 14 | Experts consensus on Chinese nomenclature of Budd-Chiari syndrome显示文摘In China,Budd-Chiari syndrome has been transliterated into six names according to the pronunciation of the letters.To standardize and unify the Chinese names of the disease,multi-disciplinary experts suggest translating Budd-Chiari syndrome into hepatic vein inferior venal cava obstruction syndrome as its Chinese name after reaching a consensus through discussion. | Maoheng Zu Ke Xu Interventional Division of Radiology Society of Chinese Medical Association | 2021 | Journal of Interventional Medicine2021，4，3： | 4 |
| 15 | Therapeutic effect of transcatheter arterial chemoembolization and percutaneous injection of acetic acids on primary liver cancer显示文摘BACKGROUND: The resection rate of primary liver tumor in China is only about 20%. A lot of patients with moderate and advanced liver tumor may lose the chance of opera- tion. The objective of present research was to study the ef- ficacy of transcatheter arterial chemoembolization (TACE) combined with percutaneous injection of chemical agents and acetic acids in the treatment of patients with primary liver cancer (PLC). METHODS: Thirty-three patients with middle and ad- vanced stage of PLC were divided into two groups: percu- taneous injection of chemical agents and acetic acids (15 patients, group A) and TACE (18 patients, group B). RESULTS: Tumor diameter and serum AFP level reduced to 86.6% and 83.3% in group A, and 55.5% and 40% in group B, respectively. There was significant difference be- tween the two groups ( P < 0 . 0 1 ) . The 1-, 2-, 3-, 4-year survival rates of group A were 96.7%, 86.6%, 51.3%, 33.3%, respectively and in group B were 66.7%, 44.4%, 16.7%, 0%, respectively (P<0.01). CONCLUSION: TACE combined with percutaneous injec- tion of chemical agents and acetic acids is efficacious to in- crease the survival rate of patients with PLC. | Hong-Bin Chen, Yue Huang, Dong-Ling Dai, Xia Zhang, Zhong-Wen Huang, Qi-Kai Zhang, Hui-Hua Wang, Jun-Su Zhang and Ge Pan Sanming, China Departments of Gastroenterology , Interventional Therapy , Ultrasonography and CT , Sanming Municipal First Hospital, Sanming 365000, China Department of Gastroenterology, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400000, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，1： | 4 |
| 16 | Induced pluripotent stem cell-related genes influence biological behavior and 5-fluorouracil sensitivity of colorectal cancer cells显示文摘Objective:We aimed to perform a preliminary study of the association between induced pluripotent stem cell(iPS)-related genes and biological behavior of human colorectal cancer(CRC) cells,and the potential for developing anti-cancer drugs targeting these genes.Methods:We used real-time reverse transcriptase polymerase chain reaction(RT-PCR) to evaluate the transcript levels of iPS-related genes NANOG,OCT4,SOX2,C-MYC and KLF4 in CRC cell lines and cancer stem cells(CSCs)-enriched tumor spheres.NANOG was knockdowned in CRC cell line SW620 by lentiviral transduction.3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assays,plate colony formation,and a mouse xenograft model were used to evaluate alterations in biological behavior in NANOG-knockdown SW620 cells.Also,mock-knockdown and NANOG-knockdown cells were treated with 5-fluorouracil(5-FU) and survival rate was measured by MTT assay to evaluate drug sensitivity.Results:A significant difference in the transcript levels of iPS-related genes between tumor spheres and their parental bulky cells was observed.NANOG knockdown suppressed proliferation,colony formation,and in vivo tumorigenicity but increased the sensitivity to 5-FU of SW620 cells.5-FU treatment greatly inhibited the expression of the major stemness-associated genes NANOG,OCT4,and SOX2.Conclusions:These results collectively suggest an overlap between iPS-related genes and CSCs in CRC.Quenching a certain gene NANOG may truncate the aggressiveness of CRC cells. | Zhong SHI1,Rui BAI1,Zhi-xuan FU1,Yong-liang ZHU2,Rong-fu WANG3,Shu ZHENG1(1Cancer Institute(Key Laboratory of Cancer Prevention and Intervention,China National Ministry of Education,Key Laboratory of Molecular Biology in Medical Sciences,Zhejiang Province,China),the Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China)(2Department of Gastroenterology,the Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China)(3Center for Cell and Gene Therapy,Department of Pathology and Immunology,Baylor College of Medicine,Houston,Texas 77030,USA) | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，1： | 4 |
| 17 | A novel arterial pouch model of saccular aneurysm by concomitant elastase and collagenase digestion显示文摘Background: An ideal aneurysm model of cerebral aneurysm is of great importance for studying the pathogenesis of the lesion and testing new techniques for diagnosis and treatment. Several models have been created in rabbits and are now widely used in experimental studies; however, every model has certain intrinsic limitations. Here we report the development of a novel saccular aneurysm model in rabbits using an arterial pouch that is subject to in vitro pre-digestion with combined elastase and collagenase. Methods: A segment of right common carotid artery (CCA) was dissected out and treated with elastase (60 U/ml, 20 min) followed by type I collagenase (1 mg/ml, 15 min) in vitro. The graft was anastomosed to an arterial arch built with the left CCA and the remaining right CCA, while the other end of the graft was ligated. The dimension and tissue structure of the pouch were analysed immediately, 2 or 8 weeks after operation. Findings: Ten terminal aneurysms were produced. The gross mor-phology of the aneurysm resembles the human cerebral terminal aneurysms. We have observed the following pathological changes: (1) growth of the aneurysm (mean diameter increased from (2.0±0.1) to (3.2±0.3) mm at 2 weeks, P<0.001, n=7~10); (2) thinning of the aneurysmal wall (the mean wall thickness decreased to 44% at 2 weeks), which was accompanied by significant losses of elastic fibres, collagen and the cellular component; and (3) spontaneous rupture (3 out of 9, one aneurysm ruptured 24 h after operation with the other two at 2 and 4 weeks respectively). Conclusion: This rabbit arterial pouch model mimics human cerebral aneurysms in relation to morphology and histology. In particular, this model exhibited an increased tendency of spontaneous rupture. | YANG Xin-jian, LI Li, WU Zhong-xue (Department of Interventional Neuroradiology, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China) | 2007 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2007，8，10： | 3 |
| 18 | One patient with metastastic colorectal cancer successfully treated by combination of targeted agents after failure of chemotherpay显示文摘Either cetuximab or bevacizumab can improve the survival of patients with metastastic colorectal cancer (mCRC) if administered combided with cytotoxic agents. However, the effect of two or more target agents in combination is uncertain in these patients. Here, we reported a patient with mCRC successfully treated by a combination of target agents after the failure of chemotherapy. The patient received palliative resection of primary tumor followed by 9 cycles of postoperative XELOX regimen, cytokine-induced killer cell (CIK)-based biotherapy, traditional Chinese medicine, particle implantation in the lung metastatic lesions. The tumor progressed 20 months after the standard treatments. Then, the regimen cetuximab, bevacizumab and cefitinib was applied. During the treatment with targeted agents, grade IV acne-like rash and relatively severe parionychia of the toes occurred. Both of them recovered smoothly. The PET-CT reexamination at 40 days after the target treatment showed that the metabolism of mediastinal lymph nodes basically recovered to a normal level. The combination of multiple targeted agents obtained a progression-free survival(PFS) of 11 months and the patient with a good quality of life during this period. | Liang-Ping Xia1,2, Pei-Hong Wu1,3, Jian-Chuan Xia1,4, Bei Zhang1,2, Zhong-Zhen Guan1,5, De-Sen Wan1,6, Gui-Fang Guo1,2, Yi-Xin Zeng1,7 1 State Key Laboratory of Oncology in South China, Guangzhou, Guangdong 510060, P. R. China 2 VIP Region, Sun Yat-sen Universty Cancer Center, Guangzhou, Guangdong 510060, P. R. China 3 Department of Medical Imaging and Interventional Radiology, Sun Yat-sen Universty Cancer Center, Guangzhou, Guangdong 510060, P. R. China 4 Biotherapy Cancer, Sun Yat-sen Universty Cancer Center, Guangzhou, Guangdong 510060, P. R. China 5 Department of Medical Oncology, Sun Yat-sen Universty Cancer Center, Guangzhou, Guangdong 510060, P. R. China 6 Department of Colorectal Cancer,Sun Yat-sen Universty Cancer Center, Guangzhou, Guangdong 510060, P. R. China 7 Sun Yat-sen Universty Cancer Center, Guangzhou, Guangdong 510060, P. R. China | 2010 | Chinese Journal of Cancer2010，29，12： | 3 |
| 19 | Outcomes of loco-regional therapy for down-staging of hepatocellular carcinoma prior to liver transplantation显示文摘BACKGROUND: The number of loco-regional therapies (LRTs) for hepatocellular carcinoma (HCC) has increased dramatically during the past decade. Many patients with HCC who were beyond the Milan criteria were allowed to receive a liver transplantation (LT) once the HCC was successfully down-staged. This retrospective study aimed to analyze the outcomes of LRTs prior to LT in patients with HCC beyond the Milan criteria. METHODS: We analyzed 56 patients treated from June 2006 to March 2010: 22 met the Milan criteria (T1+T2, 39.3%), 16 had T3 tumors (28.6%), and 11 had T4a tumors (19.6%), while 7 were suspected of tumor vascular invasion (T4b, 12.5%). All patients underwent preoperative LRTs, including transcatheter arterial chemoembolization, radiofrequency ablation, percutaneous ethanol injection, liver resection, and/or microwave coagulation therapy. The number of the patients who were successfully down-staged before LT, the types of LRTs used before LT, and their outcomes after LT were recorded. RESULTS: Eleven patients had necrotic tumors (pT0, 19.6%); 6 had pT1 tumors (10.7%), 22 had pT2 tumors (39.3%), 6 had pT3 tumors (10.7%), 5 had pT4a tumors (8.9%), and 6 had pT4b tumors (10.7%). The histopathologic tumors of 39 patients (69.6%) were down-staged and met the established Milan criteria (pT0-2). Imaging-proven under-staging was present in 5 HCC patients (8.9%) who had tumors involving the intrahepatic venous system. Twenty-three patients (41.1%) had stable HCC and 10 (17.9%) died. The 1-, 3- and 4-year survival rates were 96%, 73% and 61%, respectively, with a mean survival time of 22.29±1.63 months. Six patients died of tumorrecurrence. The 1-, 3- and 4-year recurrence-free survival (RFS) rates were 88%, 75% and 66%, respectively. The 3-year RFS of patients with pT0-2 tumors was 82%, which was markedly greater than that of patients with pT3 tumors (63%, P=0.018) or pT4 tumors (17%, P=0.000). Although the 3-year RFS of patients with pT3 tumors was greater than that of patients with pT4 tumors, the difference was not significant. CONCLUSIONS: Successful down-staging of HCCs can be achieved in the majority of carefully selected patients by LRTs. Importantly, patients who are successfully down-staged and undergo LT may have a higher RFS rate. | Xian-Jie Shi, Xin Jin, Mao-Qiang Wang, Li-Xin Wei, Hui-Yi Ye, Yu-Rong Liang, Ying Luo and Jia-Hong DongDepartment of Hepatobiliary Surgery Department of Intervention Radiology Department of Pathology and Department of Radiology General Hospital of PLA, Beijing 100853, China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，2： | 3 |
| 20 | Chinese clinical practice guidelines for ultrasound-guided irreversible electroporation of liver cancer(version 2022)显示文摘Introduction Liver cancer remains a global health challenge,and its incidence is increasing worldwide.It is estimated that by 2025,more than one million individuals will be affected by liver cancer annually[1,2].In recent years,ablation has become a widely accepted treatment option for patients with primary and secondary liver malignancies[3].The commonly used ablation method for liver cancer is thermal ablation,including radiofrequency ablation. | Min Xu Li-Ting Xie Yue-Yong Xiao Ping Liang Qi-Yu Zhao Zhong-Min Wang Wei-Lu Chai Ying-Tian Wei Lin-Feng Xu Xiao-Kun Hu Ming Kuang Li-Zhi Niu Chen-Guo Yao Hai-Ying Kong Guo Tian Xiao-Yan Xie Xin-Wu Cui Dong Xu Jun Zhao Tian-An Jiang The Interventional Diagnosis and Treatment Group of the Ultrasonic Medicine Branch of Chinese Medical Association,The Nanoknife Tumor Ablation Society of the Interventional Minimally Invasive Therapy Special Committee of China Medicine Education Association | 2022 | Hepatobiliary & Pancreatic Diseases International2022，21，5： | 2 |
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