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| 1 | Analysis of hepatitis B virus preS1 variability and prevalence of the rs2296651 polymorphism in a Spanish population显示文摘AIM To determine the variability/conservation of the domain of hepatitis B virus(HBV) pre S1 region that interacts with sodium-taurocholate cotransporting polypeptide(hereafter, NTCP-interacting domain) and the prevalence of the rs2296651 polymorphism(S267 F, NTCP variant) in a Spanish population. METHODS Serum samples from 246 individuals were included and divided into 3 groups: patients with chronic HBV infection(CHB)(n = 41, 73% Caucasians), patients with resolved HBV infection(n = 100, 100% Caucasians) and an HBV-uninfected control group(n = 105, 100% Caucasians). Variability/conservation of the amino acid(aa) sequences of the NTCPinteracting domain,(aa 2-48 in viral genotype D) and a highly conserved pre S1 domain associated with virion morphogenesis(aa 92-103 in viral genotype D) were analyzed by next-generation sequencing and compared in 18 CHB patients with viremia > 4 log IU/mL. The rs2296651 polymorphism was determined in all individuals in all 3 groups using an in-house real-time PCR melting curve analysis.RESULTS The HBV pre S1 NTCP-interacting domain showed a high degree of conservation among the examined viral genomes especially between aa 9 and 21(in the genotype D consensus sequence). As compared with the virion morphogenesis domain, the NTCPinteracting domain had a smaller proportion of HBV genotype-unrelated changes comprising > 1% of the quasispecies(25.5% vs 31.8%), but a larger proportion of genotype-associated viral polymorphisms(34% vs 27.3%), according to consensus sequences from Gen Bank patterns of HBV genotypes A to H. Variation/conservation in both domains depended on viral genotype, with genotype C being the most highly conserved and genotype E the most variable(limited finding, only 2 genotype E included). Of note, proline residues were highly conserved in both domains, and serine residues showed changes only to threonine or tyrosine in the virion morphogenesis domain. The rs2296651 polymorphism was not detected in any participant.CONCLUSION In our CHB population, the NTCP-interacting domain was highly conserved, particularly the proline residues and essential amino acids related with the NTCP interaction, and the prevalence of rs2296651 was low/null. | Rosario Casillas David Tabernero Josep Gregori Irene Belmonte Maria Francesca Cortese Carolina González Mar Riveiro-Barciela Rosa Maria López Josep Quer Rafael Esteban Maria Buti Francisco Rodríguez-Frías | 2018 | World Journal of Gastroenterology2018，24，6： | 5 |
| 2 | Mitochondrial markers for the detection of four duck species and the specific identification of Muscovy duck in meat mixtures using the polymerase chain reaction显示文摘 | Irene Martín Teresa García Violeta Fajardo Inés López-Calleja María Rojas Pablo E. Hernández Isabel González Rosario Martín | 2007 | Meat Science2007，，4： | 2 |
| 3 | Identification of an opd (Organophosphate Degradation) Gene in an Agrobacterium Isolate显示文摘 | Irene Horne Tara D S Rebecca L | 2002 | Applied and Environmental Microbiology2002，68，7： | 1 |
| 4 | 16S rRNA and 16S to 23S internal transcribed spacer sequence analysisreveal inter and intraspecific Bifidobacteriumphylogeny显示文摘 | Nathalie L B Herve P Irene M | 1996 | lntem J Syst Bacteriology1996，46，： | 1 |
| 5 | Influences of redox transformation, metal complexation and aggregation of fulvic acid and humic acid on Cr( Ⅵ) and As ( V ) removal by zero-valent iron 显示文摘 | MARK S H M IRENE M C L | 2011 | Chemosphere2011，84，： | 1 |
| 6 | 16S rRNA and 16S to 23 S internal transcribed spacer sequence analysis reveal inter and intraspecific Bifidobacterium phylogeny显示文摘 | Nathalie L B Helve P Irene M | 1996 | Intern J Syst Bacteriology1996，46，： | 1 |
| 7 | Comparison of calcium chloride and calcium lactate effectiveness in maintaining shelf stability and quality of fresh-cut cantaloupes 显示文摘 | IRENE L DIANE M | 2000 | Postharvest Biology and Technology2000，91，： | 1 |
| 8 | Communication in Europe on semicarbazide and baby food 显示文摘 | Anne L G Irene V G | 2006 | Journal of Fish Research2006，9，8： | 1 |
| 9 | 16S rRNA to 23S internal transcribed spacer sequence analyses reveal inter - and intraspecific Bifidobacterium phylogeny 显示文摘 | Nathalie L B Herve P Irene M | 1996 | International Journal of Systematic and Evolutionary Microbiology1996，46，1： | 1 |
| 10 | Urban environmental quality and human well--being towards a conceptual framework and demarcation of concepts: a literature study 显示文摘 | Irene K Kees L Gooitske M | 2003 | Landscape and Urban Planning2003，65，： | 1 |
| 11 | Aspartate aminotransferase in synaptic and nonsynaptic mitochondria: Differential effect of compounds that influ-ence transient hetero-enzyme complex (metabolon) format-ion 显示文摘 | Mary C Mckenna Irene B Hopkins Steven L | 2006 | Neurochemistry International2006，48，62： | 1 |
| 12 | 16S rRNA and 16S to 23S internal transcribed spacer sequence analysis reveal inter and inlraspeeific Bifidobaeterium phylogeny 显示文摘 | Nathalie L B Herve P Irene M | 1996 | Int J Sys Baeteriol1996，46，1： | 1 |
| 13 | FGF-4 or FGF-2 administration induces apoptosis,collagen type I expression,and mineralization in the developing coronal suture显示文摘 | Hans-van L Christl VK | 2001 | J Craniofac Surg2001，12，： | 1 |
| 14 | A Jess-en- abled context elicitation system for providing context-aware Web services显示文摘 | Yang Stephen J H Zhang Jia Chen Irene Y L | 2008 | Expert Systems with Application2008，34，4： | 1 |
| 15 | Peripheral intravenous therapy: key risks and implications for practlce显示文摘 | Paula I Irene L | 2005 | Nursing Standard2005，46，19： | 1 |
| 16 | Rhizoremediation: A beneficial plant-microbe interaction显示文摘 | IRENE Kuiper ELLEN L L GUIDO V B | 2004 | Mol PlantMicrobe Interact2004，17，1： | 1 |
| 17 | Effect ofillumination on the display life of fresh pork sausages packagedmodified atmosphere influence of the addition of rosemary,ascorbic acid and black pepper 显示文摘 | Martinez L Irene Cilia Jose Antonio Beltran | 2007 | Meat Science2007，，75： | 1 |
| 18 | 1,4-and 2,4-substituted-1,2,3-triazoles as potential potassium channel activators(Ⅶ)显示文摘 | Vincenzo C Irene G Oreste L | 2005 | Farmaco2005，60，： | 1 |
| 19 | Texture evolution in equal-channel angular extrusion显示文摘 | Irene J. Beyerlein László S. Tóth | 2009 | Progress in Materials Science2009，，4： | 1 |
| 20 | In vivo chromatin remodeling events leading to inflammatory gene transcription under diabetic conditions 显示文摘 | MIAO F IRENE GG LINDA L | 2004 | J Biol Chem2004，279，18： | 1 |
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