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| 1 | Prolonged high-fat-diet feeding promotes non-alcoholic fatty liver disease and alters gut microbiota in mice显示文摘BACKGROUND Non-alcoholic fatty liver disease (NAFLD) has become an epidemic largely due to the worldwide increase in obesity. While lifestyle modifications and pharmacotherapies have been used to alleviate NAFLD, successful treatment options are limited. One of the main barriers to finding safe and effective drugs for long-term use in NAFLD is the fast initiation and progression of disease in the available preclinical models. Therefore, we are in need of preclinical models that (1) mimic the human manifestation of NAFLD and (2) have a longer progression time to allow for the design of superior treatments. AIM To characterize a model of prolonged high-fat diet (HFD) feeding for investigation of the long-term progression of NAFLD. METHODS In this study, we utilized prolonged HFD feeding to examine NAFLD features in C57BL/6 male mice. We fed mice with a HFD (60% fat, 20% protein, and 20% carbohydrate) for 80 wk to promote obesity (Old-HFD group, n = 18). A low-fat diet (LFD)(14% fat, 32% protein, and 54% carbohydrate) was administered for the same duration to age-matched mice (Old-LFD group, n = 15). An additional group of mice was maintained on the LFD (Young-LFD, n = 20) for a shorter duration (6 wk) to distinguish between age-dependent and age-independent effects. Liver, colon, adipose tissue, and feces were collected for histological and molecular assessments.RESULTS Prolonged HFD feeding led to obesity and insulin resistance. Histological analysis in the liver of HFD mice demonstrated steatosis, cell injury, portal and lobular inflammation and fibrosis. In addition, molecular analysis for markers of endoplasmic reticulum stress established that the liver tissue of HFD mice have increased phosphorylated Jnk and CHOP. Lastly, we evaluated the gut microbial composition of Old-LFD and Old-HFD. We observed that prolonged HFD feeding in mice increased the relative abundance of the Firmicutes phylum. At the genus level, we observed a significant increase in the abundance of Adercreutzia, Coprococcus, Dorea, and Ruminococcus and decreased relative abundance of Turicibacter and Anaeroplasma in HFD mice. CONCLUSION Overall, these data suggest that chronic HFD consumption in mice can mimic pathophysiological and some microbial events observed in NAFLD patients. | Kandy T Velázquez Reilly T Enos Jackie E Bader Alexander T Sougiannis Meredith S Carson Ioulia Chatzistamou James A Carson Prakash S Nagarkatti Mitzi Nagarkatti E Angela Murphy | 2019 | World Journal of Hepatology2019，11，8： | 6 |
| 2 | Cytokine gene polymorphisms and graft-versus-host disease in children after matched sibling hematopoietic stem cell transplantation:a single-center experience显示文摘Various polymorphisms in cytokine genes have recently been investigated as candidate risk factors in allogeneic hematopoetic stem cell transplantation(allo-HSCT).We retrospectively analyzed specific polymorphisms in genes for interleukin(IL)-10,IL-6,tumor-necrosis factor alpha(TNF-α)and interferon gamma(IFN-c)in a pediatric cohort of 57 histocompatibility leucocyte antigen(HLA)-identical sibling myeloablative transplants.Both recipient and donor genotypes were tested for association with graft-versus-host disease(GVHD)by statistical methods including Cox regression analysis.We found a significant association between the IL-10 promoter haplotype polymorphisms at positions-1082,-819 and-592 with the occurrence of severe(grades Ⅲ–Ⅳ)acute GVHD(aGVHD).Recipients with the haplotype GCC had a statistically significant decreased risk of severe aGVHD(hazard risk(HR)50.20,95% confidence interval(CI):0.06–0.67)in comparison with patients with other IL-10 haplotypes(P50.008).Transplant-related mortality at 1 year was significantly lower in recipients with this haplotype(HR50.17,95% CI:0.012–0.320)versus other IL-10 haplotypes(P=0.03),whereas overall survival was not influenced by IL-10 haplotype polymorphisms.In multivariate analysis,the presence of the IL-10 GCC haplotype was found as the only variable associated with a statistically significant decreased hazard of severe aGVHD development(P=0.02,HR50.21,95% CI:0.05–0.78).These results suggest that pediatric patients possessing the IL-10 GCC haplotype may be protected from the occurrence of severe aGVHD in the setting of matched sibling HSCT. | Evgenios Goussetis Ioanna Varela Ioulia Peristeri Vasiliki Kitra Kleopatra Spanou Olga Moraloglou Anna Paisiou Sofia Karatasaki Alexandra Soldatou Nikki Constantinidou Stelios Graphakos | 2011 | Cellular & Molecular Immunology2011，8，3： | 2 |
| 3 | Inventory control of spare parts using a bayesian approach:a case study显示文摘 | KOSTAS-PLATON ARONIS IOULIA MAGOU ROMMERT DEKKER GEORGE TAGARAS | | 0，，3： | 1 |
| 4 | Benefits and cost implica-tions from integrating small flexible nuclear reactors with off-shore wind farms in a virtual power plant显示文摘 | S Arturs P Ioulia P | | 0，，01： | 1 |
| 5 | Benefits and cost implications from integrating small flexible nuclear reactors with off-shore wind farms in a virtual power plant显示文摘 | David Shropshire Arturs Purvins Ioulia Papaioannou Isabella Maschio | 2012 | Energy Policy2012，，： | 1 |
| 6 | Sonophotocatalytic treatment of ofloxacin in secondary treated effluent and elucidation of its transformation products 显示文摘 | Evroula H Ioulia F Despo F K | 2013 | Chemical Engineering Journal2013，224，： | 1 |
| 7 | Inventory control of spare parts using a Bayesian approach:a case study显示文摘 | Kostas-Platon Aronis Ioulia Magou Rommert Dekker George Tagaras | 2004 | European Journal of Operational Research2004，154，： | 1 |
| 8 | Dimensional changes, gel layer evolu tion and drug release studies in hydrophilic matrices loaded with drugs of different solubility 显示文摘 | Manuel E Ioulia P Marilena V | 2007 | Int J Pharm2007，339，12： | 1 |
| 9 | Glucose and tolbutamide induce apoptosis in pancreatic-cells: a process dependent on intracellular Ca2+ concentration显示文摘 | Sergei VZ Boris Z Martin K Sten O Per-Olof B | 1998 | J Biol Chem1998，273，33： | 1 |
| 10 | Inventory control of spare parts using a Bayesian approach: a case study 显示文摘 | Kostas-Platon Aronis Ioulia Magou Rommert Dekker George Tagaras | 2004 | European Journal of Operational Research2004，154，： | 1 |
| 11 | Evaluation of boronic acid disk tests for differentiating KPC - possessing Klebsiella pneumoniae isolales in the clinical laboratory 显示文摘 | Athanassios Tsakris Ioulia Kristo Aggeliki Poulou | 2009 | J Clin Microbial2009，47，2： | 1 |
| 12 | Recent progress on terahertz generation based on difference frequency generation:from power scaling to compact and portable sources显示文摘The progress achieved on power scaling and compact and portable THz sources is reviewed.By reversely stacking the GaP plates,the photon conversion efficiency is improved from 25% to 40% which corresponds to the maximum value.When the number of the plates is increased from four to five,the output power decreases because of back conversion.The THz generation is also investigated by mixing the two frequencies generated by a single Nd:YLF solid-state laser.The average output power reaches 1 μW.The introduction of two Nd:YLF crystals significantly improves the output power to 4.5 μW.This configuration facilitates the generation of different output frequencies. | Yujie J.Ding Pu Zhao Srinivasa Ragam Da Li Ioulia B.Zotova | 2011 | Chinese Optics Letters2011，9，11： | 1 |
| 13 | Effects of atorvastatin on apelin, visfatin (nampt), ghrelin and early carotid atherosclerosis in patients with type 2 diabetes显示文摘 | Nikolaos Kadoglou Nikolaos Sailer Alkistis Kapelouzou Stylianos Lampropoulos Ioulia Vitta Alkiviadis Kostakis Christos Liapis | 2012 | Acta Diabetologica2012，，4： | 1 |
| 14 | Modeling and field measurements of ph0tovoltaic units connected to LV grid显示文摘 | Ioulia T P Minas C A Charis S D | 2011 | IEEE Transactions on Power Delivery2011，，： | 1 |
| 15 | Asymmetric transesterification of secondary alcohols catalyzed by feruloyl esterase from Humicola insolens显示文摘 | Nikos S Hatzakis Ioulia Smonou | | 0，，4： | 1 |
| 16 | Inventory Control ofSpare Parts Using a Bayesian Approach:A Case Study显示文摘 | Kostas-Platon A Ioulia M Rommert D etal | 2004 | European Journal ofOperational Research2004，154，： | 1 |
| 17 | Mechanistic Studies of Mini-TAR RNA/DNA Annealing in the Absence and Presence of HIV-1 Nucleocapsid Protein显示文摘 | My-Nuong Vo George Barany Ioulia Rouzina Karin Musier-Forsyth | 2006 | Journal of Molecular Biology2006，，： | 1 |
| 18 | Modulation of p53 Function by SET8-Mediated Methylation at Lysine 382显示文摘 | Xiaobing Shi Ioulia Kachirskaia Hiroshi Yamaguchi Lisandra E. West Hong Wen Evelyn W. Wang Sucharita Dutta Ettore Appella Or Gozani | 2007 | Molecular Cell2007，，4： | 1 |
| 19 | Thermodynamics of DNA Binding and Condensation:Isothermal Titration Calorimetry and Electrostatic Mechanism显示文摘 | DAUMANTAS MATULIS IOULIA ROUZINA BLOOMFIELD VICTOR A | 2000 | J Mol Biol2000，296，： | 1 |
| 20 | Immune and microRNA responses to Helicobacter muridarum infection and indole-3-carbinol during colitis显示文摘BACKGROUND Indole-3-carbinol(I3C)and other aryl hydrocarbon receptor agonists are known to modulate the immune system and ameliorate various inflammatory and autoimmune diseases in animal models,including colitis induced by dextran sulfate sodium(DSS).MicroRNAs(miRNAs)are also gaining traction as potential therapeutic agents or diagnostic elements.Enterohepatic Helicobacter(EHH)species are associated with an increased risk of inflammatory bowel disease,but little is known about how these species affect the immune system or response to treatment.AIM To determine whether infection with an EHH species alters the response to I3C and how the immune and miRNA responses of an EHH species compare with responses to DSS and inflammatory bowel disease.METHODS We infected C57BL/6 mice with Helicobacter muridarum(H.muridarum),with and without DSS and I3C treatment.Pathological responses were evaluated by histological examination,symptom scores,and cytokine responses.MiRNAs analysis was performed on mesenteric lymph nodes to further evaluate the regional immune response.RESULTS H.muridarum infection alone caused colonic inflammation and upregulated proinflammatory,macrophage-associated cytokines in the colon similar to changes seen in DSS-treated mice.Further upregulation occurred upon treatment with DSS.H.muridarum infection caused broad changes in mesenteric lymph node miRNA expression,but colitis-associated miRNAs were regulated similarly in H.muridarum-infected and uninfected,DSS-treated mice.In spite of causing colitis exacerbation,H.muridarum infection did not prevent disease amelioration by I3C.I3C normalized both macrophage-and T cell-associated cytokines.CONCLUSION Thus,I3C may be useful for inflammatory bowel disease patients regardless of EHH infection.The miRNA changes associated with I3C treatment are likely the result of,rather than the cause of immune response changes. | Rasha Raheem Alkarkoushi Yvonne Hui Abbas S Tavakoli Udai Singh Prakash Nagarkatti Mitzi Nagarkatti Ioulia Chatzistamou Marpe Bam Traci L Testerman | 2020 | World Journal of Gastroenterology2020，26，32： | 0 |
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