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| 1 | Ultrafast photonic PCR显示文摘Nucleic acid amplification and quantification via polymerase chain reaction(PCR)is one of the most sensitive and powerful tools for clinical laboratories,precision medicine,personalized medicine,agricultural science,forensic science and environmental science.Ultrafast multiplex PCR,characterized by low power consumption,compact size and simple operation,is ideal for timely diagnosis at the point-of-care(POC).Although several fast/ultrafast PCR methods have been proposed,the use of a simple and robust PCR thermal cycler remains challenging for POC testing.Here,we present an ultrafast photonic PCR method using plasmonic photothermal light-to-heat conversion via photon–electron–phonon coupling.We demonstrate an efficient photonic heat converter using a thin gold(Au)film due to its plasmon-assisted high optical absorption(approximately 65%at 450 nm,the peak wavelength of heat source light-emitting diodes(LEDs)).The plasmon-excited Au film is capable of rapidly heating the surrounding solution to over 150℃ within 3 min.Using this method,ultrafast thermal cycling(30 cycles;heating and cooling rate of 12.7960.93℃ s^(-1) and 6.660.29℃ s^(-1),respectively)from 55℃(temperature of annealing)to 95℃(temperature of denaturation)is accomplished within 5 min.Using photonic PCR thermal cycles,we demonstrate here successful nucleic acid(λ-DNA)amplification.Our simple,robust and low cost approach to ultrafast PCR using an efficient photonic-based heating procedure could be generally integrated into a variety of devices or procedures,including on-chip thermal lysis and heating for isothermal amplifications. | Jun Ho Son Byungrae Cho SoonGweon Hong Sang Hun Lee Ori Hoxha Amanda J Haack Luke P Lee | 2015 | Light(Science & Applications)2015，4，1： | 2 |
| 2 | Practical nonsingular terminal sliding-mode control of robot manipulators for high- accuracy tracking control显示文摘 | Jin M L Lee J Hun P | 2009 | IEEE Trans on Industrial Electronics2009，56，9： | 1 |
| 3 | Generating situation aware- ness for time critical decision making 显示文摘 | TINGS P ZHOU S HUN | 2011 | Transactions on Computational Science XII2011，6670，： | 1 |
| 4 | SSR and STS markers for wheat stripe rust resistance gene Yr26显示文摘 | Wang C M Zhang Y P Hun D J Kang Z S Li G P Cao A Z Chen P D | | 0，，： | 1 |
| 5 | Functional outcomes after nonoperative management of fractures of the proximal hu- merus显示文摘 | Hun son B Neidenbach P de Boer P | 2009 | Shoulder Elbow Surg2009，4，： | 1 |
| 6 | Predictors of six-month major adverse cardiac events in 30-day survivors after acute myocardial infarction显示文摘 | Jang H L Hun S P Shung C C | 2009 | Am J Cardiol2009，104，2： | 1 |
| 7 | Cancer/testis antigens trigger epitheli- al-mesenchymal transition and genesis of cancer stem-like cells 显示文摘 | Yang P Hun Z Liao H | 2015 | Curr Pharm Des2015，21，10： | 1 |
| 8 | Cathepsin D products antimicrobial peptide Parasin I from histone H2A in the skin mucosa of fish显示文摘 | Ju Hun C In Yup P Hun Sik K | 2002 | The FASEB Jounral2002，16，： | 1 |
| 9 | A globally convergent method for nonlinear programming显示文摘 | | 1977 | Journal of Optimization Theory and Applications1977，22，3： | 1 |
| 10 | Guidelines for the validation and use of inununoas-savs for determining of introduced proteins in biotechnolo-gY enhanced crops and de-rived food ingredients显示文摘 | Lipton C R Dautlick J X Grothous G D Huns P L Ma-gin K M Mihaliak C A Rubio F M Stave J W | 2000 | Food Agric Immunol2000，，12： | 1 |
| 11 | Horseradish Peroxidase Immobilized in TiO2 Nanoparticle Films on Pyrolytic Graphite Elect rodes: Direct Elect rochemist ry 显示文摘 | ZHAN G Y HE P L HUN F | 2004 | Electrochim Acta2004，，49： | 1 |
| 12 | Effects of drought on grain growth显示文摘 | Galla Gher J N Biscoe P V Hun Ter B | 1976 | Nature1976，264，： | 1 |
| 13 | Course of Platelet a ac- tivation and platelet - leukocute interaction in cerebrovascular ischemia 显示文摘 | Hun P Fatch - Moghadam S Tomandl B | 2006 | Stroke2006，37，9： | 1 |
| 14 | Heterotopic Caesaarean scar pregnancy combined with intrauterine pregnancy successfully treated with embryo aspiration for selected embryo reduction:Case rqaort显示文摘 | Bih C H Jiann L H Hun S P | 2004 | Human Reproduction2004，19，2： | 1 |
| 15 | Chromatin remodeling in cardiovascular development and physiology显示文摘 | Hun P Hang C T Yang J Chang C P | | 0，，03： | 1 |
| 16 | Chromatin regulation by Brgl underlies heart muscle development and disease显示文摘 | Hang C T Yang J Hun P Cheng H L Shang C Ashley E Zhou B Chang C P | | 0，，： | 1 |
| 17 | Design and analysis of triangle phase diagram for preparation of new lithium manganese oxide solid solutions with stable layered crystal structure 显示文摘 | Soo P K Hun C M Jang J S | 2005 | Journal of Power Sources2005，146，： | 1 |
| 18 | Improved microcirculation by low - viscosity histidine - tryptophan - ketoglutarate graft flush and subsequent cold storage in university of wisconsin solution: Results of an orthotopic rat liver transplantation model 显示文摘 | OLSCHEWSKI P HUN OLD G EIPEL C | 2008 | Transpl Int2008，21，12： | 1 |
| 19 | Rapid access to enantiopure bupropion and its major metabolite by stereospe- cific nucleophilic substitution on an aketotriflate显示文摘 | Fang Q K Hun Z Grover P | 2000 | Tetradron : Asym - Metry2000，11，： | 1 |
| 20 | Neuron-specific enoluse as a marker of in vitro neuronal damage, Part I: Assessment of neuron-specific enliase marker of neuronal damage显示文摘 | Huns P Bonhomme V Collette J et at | 1995 | J Neurosurg Anesthesiol1995，5，： | 1 |
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