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| 1 | Long-term outcome of esophageal myotomy for achalasia显示文摘AIM:Modified Heller's myotomy is still the first choice for achalasia and the assessment of surgical outcomes is usually made based on the subjective sensation of patients.This study was to objectively assess the long-term outcomes of esophageal myotomy for achalasia using esophageal manometry, 24-hour pH monitoring,esophageal scintigraphy and fiberoptic esophagoscopy.METHODS:From February 1979 to October 2000, 176 patients with achalasia underwent modified Heller's myotomy, including esophageal myotomy alone in 146 patients, myotomy in combination with Gallone or Dor antirefiux procedure in 22 and 8 patients, respectively. Clinical score,pressure of the lower esophageal sphincter (LES),esophageal clearance rate and gastroesophageal reflux were determined before and i to 22 years after surgery.RESULTS: After a median follow-up of 14 years, 84.5% of patients had a good or excellent relief of symptoms,and clinical scores as well as resting pressures of the esophageal body and LES were reduced compared with preoperative values (P<0.001).However,there was no significant difference in DeMeester score between pre-and postoperative patients(P=0.51).Esophageal transit was improved in postoperative patients, but still slower than that in normal controls. The incidence of gastroesophageal reflux in patients who underwent esophageal myotomy alone was 63.6% compared to 27.3% in those who underwent myotomy and antirefiux procedure (P=-0.087). Three (1.7%) patients were complicated with esophageal cancer after surgery.CONCLUSION: Esophageal myotomy for achalasia can reduce the resting pressures of the esophageal body and LES and improve esophageal transit and dysphagia. Myotomy in combination with antireflux procedure can prevent gastroesophageal reflux to a certain extent,but further randomized studies should be carried out to demonstrate its efficacy. | Jun-FengLiu JunZhang Zi-QiangTian Qi-ZhangWang Bao-QingLi Fu-ShunWang Fu-MinCao Yue-FengZhang YongLi ZhaoFan Jian-JingHan HuiLiu | 2004 | World Journal of Gastroenterology2004，10，2： | 6 |
| 2 | Recombinant scFv Antibodies against E Protein and N Protein of Severe Acute Respiratory Syndrome Virus显示文摘Three single chain antibodies (scFv) against the proteins of severe acute respiratory syndrome coronavirus (SARS-CoV) were isolated by phage display from an scFv antibody library. Bio-panning was carried out against immobilized purified envelope (E) and nucleocapsid (N) proteins of SARS-CoV. Their binding activity and specificity to E or N protein of SARS-CoV were characterized by phage-ELISA. Two of them, B10 and C20, could recognize non-overlapping epitopes of the E protein according to the two-site binding test result. Clone A17 could recognize N protein. The sequence of the epitope or overlapping epitope of scFv antibody A17 was PTDSTDNNQNGGRNGARPKQRRPQ. The affinity (equilibrium dissociation constant, Kd) of SARS-CoV E protein was 5.7×10–8 M for B10 and 8.9×10–8 M for C20. The affinity of A17 for N protein was 2.1×10–6 M. All three scFv antibodies were purified with affinity chromatography and determined by Western blot. | HuiLIU Yan-LiDING WeiHAN Mei-YunLIU Rui-YangTIAN Sheng-LiYANG YiGONG | 2004 | Acta Biochimica et Biophysica Sinica2004，36，8： | 3 |
| 3 | High-efficiency carrier estimator for OFDM communications显示文摘 | HuiLiu UfukTureli | 1998 | IEEE Trans1998，46，4： | 1 |
| 4 | Molecu- larly imprinted polymer on ionic liquid-modified CdSe/ ZnS quantumdots for the highly selective and sensitive optosensing of tocopherol 显示文摘 | Liu Huiliu Fang Guozheu Li Changmo | 2012 | Journal of Materials Chemistry2012，22，19： | 1 |
| 5 | Organophosphorus Compound DEPBT as a Coupling Reagent for Oligopeptides and Peptoids Synthesis: Studies on Its Mechanism显示文摘Some oligopeptides and peptoids were synthesized by applying the organophosphorus compound DEPBT as a coupling reagent. D-Biotin-OOBt was obtained unexpectedly. A proposed reaction mechanism for DEPBT-mediated coupling was proved. | HuiLIU YunHuaYE 等 | 2002 | Chinese Chemical Letters2002，13，7： | 1 |
| 6 | Super plastic metallic glasses at room temperature显示文摘 | Yan Huiliu | 2007 | Science2007，1358，： | 1 |
| 7 | Smart Antennas in Wireless System:Uplink Multiuser Blind Channel and Sequence Detection显示文摘 | HuiLiu Xiu G H | 1997 | IEEE Trans on Communications1997，45，2： | 1 |
| 8 | Mill Gear Box of Intelligent Diagnosis Based on Support Vector Machine Parameters Optimization 显示文摘 | XIAO HuiLiu YONG GangXu DE YingGuo | 2014 | Applied Mechanics and Materials2014，697，85： | 1 |
| 9 | Fuzzy control model and simulation of supply air system in a test rig of low-temperature hot-water radiator system显示文摘 | Jili zhang Yongpan Chen Tianyi Zhao Huiliu | | 0，，03： | 1 |
| 10 | Generation and Characterization of C305,a Murine Neutralizing scFv Antibody That Can Inhibit BLyS Binding to Its Receptor BCMA显示文摘B-lymphocyte stimulator (BLyS) is a member of the tumor necrosis factor (TNF) family and a key regulator of B cell response. Neutralizing single-chain fragment variable (scFv) antibody against BLyS binding to its receptor BCMA has the potential to play a prominent role in autoimmune disease therapy. A phage display scFv library constructed on pill protein of MI 3 filamentous phage was screened using BLyS.After five rounds of panning, their binding activity was characterized by phage-ELISA. Nucleotide sequencing revealed that at least two different scFv gene fragments (C305 and D416) were obtained. The two different scFv gene fragments were expressed to obtain the soluble scFv antibodies, then the soluble scFv antibodies were characterized by means of competitive ELISA and in vitro neutralization assay. The results indicated that C305 is the neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA. | Mei-YunLIU WeiHAN Yan-LiDING Tian-HongZHOU Rui-YangTIAN Sheng-LiYANG HuiLIU YiGONG | 2005 | Acta Biochimica et Biophysica Sinica2005，37，6： | 1 |
| 11 | Evaluation and treatment of cancer pain显示文摘 | | 1995 | China Clinical Tumor1995，22，7： | 1 |
| 12 | Role of Mucin Lewis Status in Resistance to Helicobacter pylori Infection in Pediatric Patients显示文摘 | SaraLindén CristinaSemino‐Mora HuiLiu JamesRick AndreDubois | 2010 | Helicobacter2010，，4： | 1 |
| 13 | Regression of melanoma metastases after immunotherapy is associated with activation of antigen presentation and interferon‐mediated rejection genes显示文摘 | RafaelCarretero EnaWang Ana I.Rodriguez JenniferReinboth Maria L.Ascierto Alyson M.Engle HuiLiu Francisco M.Camacho Francesco M.Marincola FedericoGarrido TeresaCabrera | 2012 | Int. J. Cancer2012，，2： | 1 |
| 14 | Application of Phage-displayed Single Chain Antibodies in Western Blot显示文摘A phage display single chain fragment variable library constructed on pⅢ protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens. | Yan-LiDING Mei-YunLIU WeiHAN Sheng-LiYANG HuiLIU YiGONG | 2005 | Acta Biochimica et Biophysica Sinica2005，37，3： | 0 |
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