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| 1 | Targeted mutagenesis in rice using CRISPR-Cas system显示文摘 | Jin Miao Dongshu Guo Jinzhe Zhang Qingpei Huang Genji Qin Xin Zhang Jianmin Wan Hongya Gu Li-Jia Qu | 2013 | Cell Research2013，23，10： | 103 |
| 2 | Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family显示文摘血浆膜内在的蛋白质(果仁) 是 aquaporins 的一个亚科越过膜启用水的快、控制的 translocation。在这研究,我们系统地从米饭识别了并且克隆十果仁基因。基于他们编码了的氨基酸序列的类似,这些米饭果仁基因被分类进二个组并且指定了为 OsPIP1-1 到 OsPIP1-3 和 OsPIP2-1 到在玉米跟随果仁基因的名称的 OsPIP2-7。量的 RT-PCR 分析识别了三根特定并且一叶特定的 OsPIP 基因。而且,响应盐,干旱和骆驼毛的织物处理的每 OsPIP 基因的表示侧面详细被检验。OsPIP1 (OsPIP1-1 ) 或在野类型的 Arabidopsis,腌,然而并非腌更高的集中(NaCl 的 150 公里) 的处理的显示出的提高的忍耐(NaCl 的 100 公里) 和干旱(甘露糖醇的 200 公里) 的 OsPIP2 (OsPIP2-2 ) 过去表示的转基因的植物上的分析。总起来说,这些数据响应不同压力建议每 OsPIP 基因的一个不同角色,并且应该把新层加到米饭果仁基因的生理的功能的理解。 | Lei Guo Zi Yi Wang Hong Lin Wei Er Cui Jun Chen Meihua Liu Zhang Liang Chen Li Jia Qu Hongya Gu | 2006 | Cell Research2006，16，3： | 35 |
| 3 | Disruption of phytoene desaturase gene results in albino and dwarf phenotypes in Arabidopsis by impairing chlorophyll, carotenoid, and gibberellin biosynthesis显示文摘类胡萝卜素在植物和 phytoene desaturase 基因(PDS3 ) 在许多生理的过程起一个重要作用把重要的酶之一编码为类胡萝卜素生合成小径。这里,我们报导 T-DNA 插入异种 ofPDS3 基因的鉴定和分析。功能的互补证实 thepds3 异种的白化体和矮子显型源于 PDS3 基因的功能的混乱。叶绿体开发在 pds3 异种在专业版质体阶段被逮捕。进一步的分析证明高级 ofphytoene 在 pds3 异种被积累。外长的 GA (3 ) 的增加能部分救矮子显型,建议 pds3 异种的矮子显型可能由于 GAdeficiency。Microarray 和 RT-PCR 分析证明那破坏 PDS3 基因导致了涉及至少 20 条新陈代谢的小径的基因表示变化,包括在类胡萝卜素,叶绿素,和 GA 的许多基因的抑制生合成小径。我们的数据建议在 pds3 异种的积累的 phytoene 可能在某些否定反馈起一个重要作用影响多样的细胞的小径的基因表示。 | Genji Qin Hongya Gu Ligeng Ma Yiben Peng Xing Wang Deng Zhangliang Chen Li-Jia Qu | 2007 | Cell Research2007，17，5： | 39 |
| 4 | Characterization of Arabidopsis MYB transcription factor gene AtMYB17 and its possible regulation by LEAFY and AGL15显示文摘MYB transcription factors compose one of the largest transcription factor families in Arabidopsis, which play important roles in various developmental processes as well as defense responses against environmental stresses.In this study, we report the characterization of AtMYB17 gene, a putative R2R3 type MYB gene family member in Arabidopsis.AtMYB17 was found exclusively localized in nuclear, with an activation domain at its C-terminus.AtMYB17 was highly expressed in inflorescences and siliques, especially at early flower developmental stages.The level of AtMYB17 transcripts was also found to increase after imbibition during seed germination and gradually concentrate to the shoot apex.Bioinformatics analysis identified several binding sites of LEAFY(LFY) and AGL15 in the promoter re-gion of AtMYB17.Promoter-GUS fusion analysis showed that the LFY binding sites were important in fine-tuning regulation of the spatio-temporal expression of AtMYB17 in transgenic plants.Moreover, AtMYB17 was up-regulated in 35S::AGL15 plants.Taken together, our data suggest that LFY may be involved in the regulation of AtMYB17, possibly together with AGL15, and thereafter in early inflorescence development and seed germination. | Yunfei Zhang Guangyu Cao Li-Jia Qu Hongya Gu | 2009 | Journal of Genetics and Genomics2009，36，2： | 24 |
| 5 | Over-expression of WOX1 Leads to Defects in Meristem Development and Polyamine Homeostasis in Arabidopsis显示文摘In plants,the meristem has to maintain a separate population of pluripotent cells that serve two main tasks,i.e.,self-maintenance and organ initiation,which are separated spatially in meristem.Prior to our study,WUS and WUS-like WOX genes had been reported as essential for the development of the SAM.In this study,the consequences of gain of WOX1 function are described.Here we report the identification of an Arabidopsis gain-of-function mutant wox1-D,in which the expression level of the WOX1(WUSCHEL HOMEOBOX 1) was elevated and subtle defects in meristem development were observed.The wox1-D mutant phenotype is dwarfed and slightly bushy,with a smaller shoot apex.The wox1-D mutant also produced small and dark green leaves,and exhibited a failure in anther dehiscence and male sterility.Molecular evidences showed that the transcription of the stem cell marker gene CLV3 was down-regulated in the meristem of wox1-D but accumulated in the other regions,i.e.,in the root-hypocotyl junction and at the sites for lateral root initiation.The fact that the organ size and cell size in leaves of wox1-D are smaller than those in wild type suggests that cell expansion is possibly affected in order to have partially retarded the development of lateral organs,possibly through alteration of CLV3 expression pattern in the meristem.An S-adenosylmethionine decarboxylase(SAMDC) protein,SAMDC1,was found able to interact with WOX1 by yeast two-hybrid and pull-down assays in vitro.HPLC analysis revealed a significant reduction of polyamine content in wox1-D.Our results suggest that WOX1 plays an important role in meristem development in Arabidopsis,possibly via regulation of SAMDC activity and polyamine homeostasis,and/or by regulating CLV3 expression. | Yanxia Zhang Renhong Wu Genji Qin Zhangliang Chen Hongya Gu Li-Jia Qu | 2011 | Journal of Integrative Plant Biology2011，53，6： | 23 |
| 6 | Arabidopsis AtBECLIN 1/AtAtg6/AtVps30 is essential for pollen germination and plant development显示文摘兼容丑恶纸巾的表面上的花粉萌芽是为植物授精的必要的步。这里,我们报导 Arabidopsis 变异的 bcl1 是由于花粉萌芽的失败不育的男性。我们证明 bcl1 异种等位基因不能被男配偶体播送,没有同型结合的 bcl1 异种被获得。花粉发育阶段的分析显示 bcl1 变化影响花粉萌芽然而并非花粉成熟。分子的分析证明花粉萌芽的失败被 AtBECLIN 1 的混乱引起。AtBECLIN 1 在成熟花粉主要被表示并且与重要相同编码蛋白质到在酵母为 autophagyand vacuolar 蛋白质排序的过程(VPS ) 要求的 Beclin1/Atg6/Vps30。我们也证明 AtBECLIN 1 为正常被要求植物开发,和那与 autophagy, VPS 和 glycosylphosphatidylinositolanchor 系统有关的基因,被 AtBECLIN 1 的缺乏影响。 | Genji Qin Zhiqiang Ma Li Zhang Shufan Xing Xianhui Hou Jie Deng Jingjing Liu Zhangliang Chen Li-Jia Qu Hongya Gu | 2007 | Cell Research2007，17，3： | 17 |
| 7 | Disruption of the 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) gene results in albino, dwarf and defects in trichome initiation and stomata closure in Arabidopsis显示文摘1-Deoxy-D-xylulose-5-phosphate reductoisomerase (DXR ) 是涉及 2-C-methyl-D-erythritol-4-phosphate (MEP ) 的重要的酶为 isoprenoid 生合成提供基本五碳的单位的小径。在植物开发和新陈代谢调查 MEP 小径的角色,我们在 dxr 异种(GK_215C01 ) 和二根 DXR 转基因的合作抑制线上执行了详细分析, OX-DXR-L2 和 OX-DXR-L7。我们发现 dxr 异种是白化体和矮子。它从来没跑,显著地减少了毛状体的数字,大多数 stomata 不能在叶子通常关门。二根合作抑制线没有毛状体生产了更黄的开花期和白化体萼片。涉及毛状体开始的基因的抄写层次被发现强烈被假装,包括 GLABRA1,透明种皮光洁 1, TRIPTYCHON 并且细长纤弱,哪个被 gibberellic 酸(气体) 调整的表示。而 abscisic 酸(骆驼毛的织物) 的外长的申请能救 stomata 闭合缺点, GA3 的外长的申请能部分救矮子显型和 dxr 的毛状体开始,建议 GA 和骆驼毛的织物的底层贡献在 dxr 异种的显型。我们进一步发现涉及 GA 和骆驼毛的织物的 biosynthetic 小径的基因并列地被调整。这些结果显示 plastidial MEP 小径的那混乱导致光合的颜料,气体和骆驼毛的织物的 biosynthetic 缺乏,并且这样发展畸形,并且从细胞质的 mevalonate 小径的流动不是足够的救缺乏,这由 plastidial MEP 小径的阻塞引起了。这些结果在控制 isoprenoids 的生合成为 MEP biosynthetic 小径揭示一个关键角色。 | Shufan Xing Jin Miao Shuang Li Genji Qin Si Tang Haoni Li Hongya Gu Li-Jia Qu | 2010 | Cell Research2010，20，6： | 17 |
| 8 | AtCDC5 regulates the G2 to M transition of the cell cycle and is critical for the function of Arabidopsis shoot apical meristem显示文摘作为一个房间周期管理者, Myb 相关的 CDC5 蛋白质被报导为在酵母和动物的房间周期的 G2 阶段必要,但是很少在植物对它的功能被知道。这里,我们在 Arabidopsisthaliana 报导 CDC5 基因的功能的描述。Arabidopsis CDC5 (AtCDC5 ) 主要与高细胞分裂活动在纸巾被表示,并且在整个胚胎形成的全部过程被表示。AtCDC5 loss-of-functionmutant 是胚胎的致命。以便调查 AtCDC5 体内的函数,我们 AtCDC5 的表达式被 RNA 干扰在减少的 generatedAtCDC5-RNAi 植物。我们发现到 M (G2/M ) 阶段转变的 G2 在 AtCDC5-RNAi 植物被影响,并且 thatendoreduplication 被增加。另外,射击顶端分生组织(SAM ) 的维护功能在 AtCDC5-RNAi 植物被扰乱,在哪个 WUSCHEL (WUS )-CLAVATA (CLV ) 和射击无分裂组织(STM ) 小径被损害。原位杂交分析证明 STM 的表示极大地在 AtCDC5-RNAi 植物的射击顶端细胞被减少。而且, cyclinB1 或 Histone4 被发现当时,在一些这些房间被表示 STMwas 的抄本无法发现。这些结果建议 AtCDC5 为 G2/M 阶段转变是必要的并且可以由控制 STM 和 WUS 的表示调整 SAM 的功能。 | Zhiqiang Lin Kangquan Yin Danling Zhu Zhangliang Chen Hongya Gu Li-Jia Qu | 2007 | Cell Research2007，17，9： | 13 |
| 9 | The molecular mechanism of SPOROCYTELESS/NOZZLE in controlling Arabidopsis ovule development显示文摘小卵为植物复制是必要的并且在授精以后发展成种子。当为在 Arabidopsis 的小卵开发的一个必要因素,而是系统程序设计语言功能的生物化学的性质仍然保持未解决, SPOROCYTELESS/NOZZLE (SPL/NZZ ) 以超过 15 年著名。这里,我们证明系统程序设计语言作为一个像适配器的 transcriptional 抑压者工作。我们证明禁止 CINCINNATA (CIN ) 的系统程序设计语言成员 TOPLESS/TOPLESS-RELATED (TPL/TPR ) 合作抑压者喜欢 TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP ) 抄写因素。我们表明系统程序设计语言在 C 终端结束使用它的耳朵主题到成员 TPL/TPRs 和它的 N 终端部分绑并且禁止 TCP。我们部分表明 TPL/TPRs 的那任何一个混乱或 TCP 的 overexpression phenocopies 在系统程序设计语言的 megasporogenesis 的缺点。而且, TCP 的混乱引起类似于 spl-D gain-of-function 异种的显型。这些结果为系统程序设计语言定义行动机制,它由镇压与 TPL/TPRs 一起控制小卵开发关键抄写因素的活动。我们的调查结果建议类似的基因压抑策略被植物和真菌采用到控制孢子。 | Baoye Wei Jinzhe Zhang Changxu Pang Hao Yu Dongshu Guo Hao Jiang Mingxin Ding Zhuoyao Chen Qing Tao Hongya Gu Li-Jia Qu Genji Qin | 2015 | Cell Research2015，25，1： | 9 |
| 10 | Variation at the transcriptional level among Chinese natural populations of Arabidopsis thaliana in response to cold stress显示文摘The Arabidopsis 25K GeneChip (ATH1, Affymetrix) was used to make a survey of the variation of the transcriptional profiles among 5 Chinese natural populations of Arabidopsis thaliana under cold treatment. In normal growth condition, the expression level of 2.26% (513 genes in the population from Jiujiang, Jiangxi, JXjjx) to 6.52% (1482 genes in the population from Tongliang, Chongqing, CQtlx) genes was 2-fold higher than that of Col ecotype. Under cold treatment, the expression of 12.84% (2920 genes in the population from Chenggu, Shaanxi, SXcgx) to 19.46% (4426 genes in the population from Qinghe, Xinjiang, XJqhx) genes was up- or down-regulated by at least two-fold that of their controls. In general, most of up-regulated genes might be the genes essential for plant surviving at low temperature, such as genes in CBF pathway and the genes responsible for synthesizing molecules accumulated for cold tolerance. However, each natural population had some specific genes induced under cold treatment. The data indicated that some of the cold-responding genes were differentiated among the populations distributed in the natural habitats with different climate conditions. CBF3, one of the key tran-scription factor genes in cold responding pathway, showed significant differences in expression among populations. The sequence analysis indicated that the changes in its regulation region caused the dramatic difference in the expression pattern. Further studies on the correlation of the function of the differentially expressed genes and the cold tolerance in different populations may provide some new insight into the molecular mechanism of adaptation to local environment in Arabidopsis thaliana in China. | HE Fei KANG JiuQing ZHOU Xin SU Zhen QU LiJia GU HongYa | 2008 | Chinese Science Bulletin2008，53，19： | 9 |
| 11 | Four Closely-related RING-type E3 Ligases,APD1-4,are Involved in Pollen Mitosis II Regulation in Arabidopsis显示文摘Ubiquitination of proteins is one of the critical regulatory mechanisms in eukaryotes.In higher plants,protein ubiquitination plays an essential role in many biological processes,including hormone signaling,photomorphogenesis,and pathogen defense.However,the roles of protein ubiquitination in the repro-ductive process are not clear.In this study,we identified four plant-specific RING-finger genes designated Aberrant Pollen Development 1 (APD1) to APD4,as regulators of pollen mitosis Ⅱ (PMⅡ) in Arabidopsis thaliana (L.).The apd1 apd2 double mutant showed a significantly increased percentage of bicellular-like pollen at the mature pollen stage.Further downregulation of the APD3 and APD4 transcripts in apd1 apd2 by RNA interference (RNAi) resulted in more severe abnormal bicellular-like pollen phenotypes than in apd1 apd2,suggesting that cell division was defective in male gametogenesis.All of the four genes were expressed in multiple stages at different levels during male gametophyte development.Confocal analysis using green florescence fusion proteins (GFP) GFP-APD1 and GFP-APD2 showed that APDs are associated with intracellular membranes.Furthermore,APD2 had E2-dependent E3 ligase activity in vitro,and five APD2-interacting proteins were identified.Our results suggest that these four genes may be involved,redundantly,in regulating the PMⅡ process during male gametogenesis. | Guo Luo Hongya Gu Jingjing Liu Li-Jia Qu | 2012 | Journal of Integrative Plant Biology2012，54，10： | 8 |
| 12 | Magnetic properties and large magnetocaloric effects in amorphous Gd-Al-Fe alloys for magnetic refrigeration显示文摘A series of the amorphous Gd75-55Al25-5Fe0-40 alloy ribbons were prepared by melt spinning.The structure,magnetic properties and magnetocaloric effect(MCE) of these alloys were investigated.The prepared samples have shown the characteristics of a second-order phase transition with zero hysteresis loss and the Tc can be tuned by changing the Fe contents.For the different compositions,the magnetic entropy change(--Sm) for a field change of 0-5 T reached a maximum value of 7.14 J kg-1 K-1 in the Gd70Al20Fe10 alloy near its Curie temperature(Tc) of 149 K.The non-linear composition dependence of(-ΔSm) could be caused by the competitions between Fe-Fe,Fe-Gd and Gd-Gd interactions.The refrigeration capacity(RC) values of these alloys are about 532-780 J/kg under a magnetic field change of 0-5 T.The results indicate that amorphous GdFeAl alloys can be considered as ideal candidates for a magnetic refrigerant in the temperature range of 104-222 K. | ZHENG ZhiGang ZHONG XiChun SU KunPeng YU HongYa LIU ZhongWu ZENG DeCheng | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，7： | 8 |
| 13 | TRANSLUCENT GREEN, an ERF Family Transcription Factor, Controls Water Balance in Arabidopsis by Activating the Expression of Aquaporin Genes显示文摘水是在几乎所有生活有机体的最丰富的分子。Aquaporins 是玩控制房间的水内容的批评 rolesin 的隧道蛋白质。这里,我们报导 AP2/EREBP 家庭抄写 factorin Arabidopsis thaliana 的鉴定,半透明的格林( TG )其在转基因的植物的 overexpression 给了提高的干旱忍耐并且使 leaves.TG 蛋白质成玻璃在原子核是局部性的,在 vitro 绑 DRE 和 GCC 元素,并且在酵母房间充当 transcriptional 使活跃之物。Microarray 分析揭示了 TG,五基因在之中编码 aquaporins 调整的 330 基因的一个总数。短暂表示试金证明 TG 直接绑在 threeaquaporin 基因的倡导者,例如 AtTIP1; 1, AtTIP2; 3,并且 AtPIP2; 2,直接显示那 TG 调整这些基因的表示。而且, AtTIP1 的 overexpression; 1 在转基因的 Arabidopsis 植物导致了使成玻璃的显型,类似于在 TG overexpression 线观察的那些。进野类型的叶子的水注射概括了 vitrifiedleaf 显型,它被从脉管的捆割掉水供应颠倒。一起拿,我们在通过直接激活 aquaporin genes.Key 词的表达式的 Arabidopsis 的数据 supportthat TG 控制水平衡: | Danling Zhu Zhe Wu Guangyu Cao Jigang Li Jia Wei Tomohiko Tsuge Hongya Gua Takashi Aoyama Li-Jia Qu | 2014 | Molecular Plant2014，7，4： | 8 |
| 14 | Duplication and divergent evolution of the CHS and CHS-like genes in the chalcone synthase(CHS)superfamily显示文摘The enzymes of the CHS-superfamily are responsible for biosynthesis of a wide range of natural products in plants. They are important for flower pigmentation, protection against UV light and defense against phytopathogens. Many plants were found to contain multiple copies of CHS genes. This review summarizes the recent progress in the studies of the CHS-superfamily, focusing on the duplication and divergent evolution of the CHS and CHS-like genes. Comparative analyses of gene structure, ex- pression patterns and catalytic properties revealed extensive differentiation in both regulation and func- tion among duplicate CHS genes. It is also proposed that the CHS-like enzymes in the CHS-superfamily evolved from CHS at different times in various or- ganisms. The CHS-superfamily thus offers a valuable model to study the rates and patterns of sequence divergence between duplicate genes. | YANG Ji GU Hongya | 2006 | Chinese Science Bulletin2006，51，5： | 7 |
| 15 | Overexpression of the Wounding-Responsive Gene AtMYB15 Activates the Shikimate Pathway in Arabidopsis显示文摘MYB 抄写因素基因在许多发展过程和植物的各种各样的防卫回答起重要作用。shikimate 小径是为三芳香的氨基酸和涉及植物的多重回答的另外的芳香的混合物的生产的一条主要 biosynthetic 小径,包括保护对紫外并且防卫。此处,我们在 Arabidopsis 把 R2R3-MYB 基因 AtMYB15 的描述描述为 shikimate 小径的使活跃之物。AtMYB15 蛋白质是原子的局部性并且一个 transcriptional 激活领域在它的 C 终端部分被发现。北污点证明 AtMYB15 是早创伤可诱导的基因。microarray 分析的 Resutls,使用量的即时聚合酶链反应证实,证明在转基因的植物的 AtMYB15 的 overexpression 导致了涉及 shikimate 小径的几乎所有基因的提高的表示。生物信息学分析证明至少一个 AtMYB15 有约束力的交流元素在这些 upregulated 基因的倡导者被检测。而且,在 shikimate 小径的这些基因也被发现被伤害导致。这些数据响应伤害作为 Arabidopsis shikimate 小径的一个可能的直接管理者建议 AtMYB15 的一个重要角色。 | Yanhui Chen Xiangbo Zhang Wei Wu Zhangliang Chen Hongya Gu Li-Jia Qu | 2006 | Journal of Integrative Plant Biology2006，48，9： | 7 |
| 16 | The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana显示文摘The transcription factors CBF1/2/3 are reported to play a dominant role in the cold responsive network of Arabidopsis by directly regulating the expression levels of cold responsive(COR) genes.In this study,we obtained CRISPR/Cas9-mediated loss-of-function mutants of cbf1$3.Over 3,000 COR genes identified by RNA-seq analysis showed a slight but significant change in their expression levels in the mutants compared to the wildtype plants after being treated at 4 °C for 12 h.The C-repeat(CRT) motif(50-CCGAC-30) was enriched in promoters of genes that were up-regulated by CBF2 and CBF3 but not in promoters of genes up-regulated by CBF1.These data suggest that CBF2 and CBF3 play a more important role in directing the cold response by regulating different sets of downstream COR genes.More than 2/3 of COR genes were co-regulated by two or three CBFs and were involved mainly in cellular signal transduction and metabolic processes;less than 1/3 of the genes were regulated by one CBF,and those genes up-regulated were enriched in cold-related abiotic stress responses.Our results indicate that CBFs play an important role in the trade-off between cold tolerance and plant growth through the precise regulation of COR genes in the complicated transcriptional network. | Yihao Shi Jiaying Huang Tianshu Sun Xuefei Wang Chenqi Zhu Yuxi Ai Hongya Gu | 2017 | Journal of Integrative Plant Biology2017，59，2： | 7 |
| 17 | The Arabidopsis Anaphase-Promoting Complex/Cyclosome Subunit 1 is Critical for Both Female Gametogenesis and Embryogenesis显示文摘Anaphase-promoting complex/cyclosome (APC/C), a multisubunit E3 ligase, plays a critical role in cell cycle control, but the functional characterization of each subunit has not yet been completed. To investigate the function of APC1 in Arabidopsis, we analyzed four mutant alleles of APC1, and found that mutation in APC1 resulted in significantly reduced plant fertility, accumulation of cyclin B, and disrupted auxin distribution in embryos. The three mutant alleles apc1-1, apc1-2 and apc1-3 shared variable defects in female gametogenesis including degradation, abnormal nuclear number, and disrupted polarity of nuclei in the embryo sac as well as in embryogenesis, in which embryos were arrested at multiple stages. All of these defects are similar to those previously identified in apc4. The mutant apc1-4, in which the T-DNA was inserted after the transmembrane domain at the C-terminus, showed much more severe phenotypes; that is, most of the ovules were arrested at the one-nucleate female gametophyte stage (stage FG1). In the apc1 apc4 double mutants, the fertility was further reduced by one-third in apc1-1/+ apc4-1/+, and in some cases no ovules even survived in siliques of apc1-4/+ apc4-1/+. Our data thus suggest that APC1, an essential component of APC/C, plays a synergistic role with APC4 both in female gametogenesis and in embryogenesis. | Yanbing Wang Yingnan Hou Hongya Gu Dingming Kang Zhang-Liang Chen Jingjing Liu Li-Jia Qu | 2013 | Journal of Integrative Plant Biology2013，55，1： | 7 |
| 18 | Molecular evolution of the exon 2 of CHS genes and the possibility of its application to plant phylogenetic analysis显示文摘The exon 2 of chalcone synthase (CHS) gene is relatively conserved during evolution. In this study, three exon 2 fragments from two species in gymnosperm (Cycas panzhihuaensis, Ginkgo biloba ) and seven from four species in angiosperm (Magnolia denudata, Salix babylonica, Nymphaea tetragona, Camellia japonica) have been amplified by PCR from genomic DNA and sequenced. Together with other 73 sequences of CHS collected from EMBL database and literature, these sequences, which embrace 19 families of gymnosperm and angiosperm, have been analyzed for their phylogenetic relations by parsimony method. The result indicated that sequences from the same systematic family usually grouped together except those from Theaceae, Magnoliaceae and Nymphaeaceae. The relative rate test revealed the rate heterogeneity of CHS genes among the families. For the nucleotide substitution the sequences from Asteraceae and Solanaceae evolve faster than those from the other families analyzed while the sequences from Poaceae, | WANG Jinling QU Lijia CHEN Jun GU Hongya CHEN Zhangliang | 2000 | Chinese Science Bulletin2000，45，19： | 6 |
| 19 | Arabidopsis AtVPS15 Plays Essential Roles in Pollen Germination Possibly by Interacting with AtVPS34显示文摘VPS15 蛋白质是在酵母和哺乳动物的房间的发展起一个枢轴的作用的 phosphatidylinositol 3-kinase 建筑群的一个部件。它在植物的相当或相同事物的功能的知识仍然保持有限。这里,我们报导那 AtVPS15,在 Arabidopsis 的酵母 VPS15p 的一个相当或相同事物,在花粉萌芽起一个必要作用。AtVPS15 的同型结合的 T-DNA 插入异种不能从 self-pollinated 的子孙被获得异质接合的异种。在 atvps15 异种和野类型的 Arabidopsis 之间的相互的十字表明 T-DNA 插入不能被男配偶体播送。显微镜的分析显示出的染色的 DAPI,亚历山大的污点和扫描电子那 atvps15 异质接合的植物生产了与野类型的花粉词法上难区分的花粉谷物,而 in vitro 萌芽实验揭示了那,花粉谷物的萌芽是有缺点的。染色表示 AtVPS15 倡导者驾驶的 GUS 记者基因的转基因的植物的分析的 GUS 显示出那 AtVPS15 主要在花粉谷物被表示。最后,二混血儿的分析表明了的 DUALmembrane 酵母那 AtVPS15 可能与 AtVPS34 直接交往。这些结果建议 AtVPS15 为花粉萌芽是很重要的,可能通过 PI3-kinase 的活动的调整。 | Wei-Ying Wang Li Zhang Shufan Xing Zhiqiang Ma Jingjing Liu Hongya Gu Genji Qin Li-Jia Qu | 2012 | Journal of Genetics and Genomics2012，39，2： | 6 |
| 20 | Experimental demonstration of ultra-large-scale terahertz all-dielectric metamaterials显示文摘All-dielectric metamaterials have emerged as a promising platform for low-loss and highly efficient terahertz devices. However, existing fabrication methods have difficulty in achieving a good balance between precision and cost. Here, inspired by the nano-template-assisted self-assembly method, we develop a micro-templateassisted self-assembly(MTAS) method to prepare large-scale, high-precision, and flexible ceramic microsphere all-dielectric metamaterials with an area exceeding 900 cm × 900 cm. Free from organic solvents, vacuum, and complex equipment, the MTAS method ensures low-cost and environmentally friendly fabrication. The ceramic microsphere resonators can be readily assembled into nearly arbitrary arrangements and complex aggregates, such as dimers, trimers, quadrumers, and chains. Finally, using the heat-shrinkable substrate and dipole coupling effect, a broadband reflector with a bandwidth of 0.15 THz and a reflection of up to 95% is demonstrated.This work provides a versatile and powerful platform for terahertz all-dielectric metamaterials, with potential to be applied in a wide variety of high-efficiency terahertz devices. | KE BI DAQUAN YANG JIA CHEN QINGMIN WANG HONGYA WU CHUWEN LAN YUPING YANG | 2019 | Photonics Research2019，7，4： | 5 |
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