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| 1 | 棉花CBF2基因克隆和超表达CBF2棉花增强抗冷性显示文摘CBF(C-repeat binding factor)是一类植物特有的转录因子,其主要功能是提高植物的抗冷性。本研究从陆地棉(Gossypium hirsutum)‘柯字棉312’中克隆到1个棉花CBF基因,命名为GhCBF2。蛋白序列分析结果表明,GhCBF2与其他植物的CBF蛋白类似,含有一个由216个氨基酸组成的AP2转录因子保守结构域。在棉花幼苗受到冷处理后,GhCBF2基因的表达水平提高。在冷胁迫处理后,过量表达GhCBF2基因棉花的存活率要显著高于野生型棉花,其细胞膜的相对渗透率显著低于野生型棉花,而可溶性糖含量显著提高。以上研究表明,GhCBF2基因可能在提高棉花抗冷性发挥作用。 | 马刘峰 陈芸 任羽 司马义.巴拉提 李玲 | 2018 | 植物生理学报2018，54，2： | 7 |
| 2 | 木本植物逆境胁迫研究进展显示文摘木本植物(Woody plant)具有多年生、木质部发达和茎坚硬等特点,能抵御复杂多变的外界环境。本综述归纳了木本植物逆境胁迫的几个重要方面,例如盐胁迫、干旱胁迫和低温胁迫。归纳并总结了与应激有关的基因、蛋白质、转录因子和应激反应机制,并简要概述了木本植物在植被保护、园林绿化和土壤改良等方面的应用价值,最后对木本植物研究的局限性和存在的不足进行了讨论。蒙古柳(Salix linearistipularis)作为松嫩平原盐碱地区唯一具有群落分布的木本植物,是研究逆境胁迫的重要树种材料。因此,本综述着重介绍了耐盐碱木本植物蒙古柳对盐等逆境胁迫的研究进展,以期为木本植物逆境胁迫研究提供一定的理论基础;并针对目前研究存在的不足,为进一步研究提供了参考方向。 | 魏芝玲 韩元进 卜媛媛 | 2020 | 分子植物育种2020，18，7： | 5 |
| 3 | The direct targets of CBFs:In cold stress response and beyond显示文摘Cold acclimation in Arabidopsis thaliana triggers a significant transcriptional reprogramming altering the expression patterns of thousands of cold-responsive(COR) genes. Essential to this process is the C-repeat binding factor(CBF)-dependent pathway, involving the activity of AP2/ERF(APETALA2/ethylene-responsive factor)-type CBF transcription factors required for plant cold acclimation. In this study, we performed chromatin immunoprecipitation assays followed by deep sequencing(ChIP-seq) to determine the genomewide binding sites of the CBF transcription factors. Cold-induced CBF proteins specifically bind to the conserved C-repeat(CRT)/dehydrationresponsive elements(CRT/DRE;G/ACCGAC) of their target genes. A Gene Ontology enrichment analysis showed that 1,012 genes are targeted by all three CBFs. Combined with a transcriptional analysis of the cbf1,2,3 triple mutant, we define 146 CBF regulons as direct CBF targets. In addition, the CBF-target genes are significantly enriched in functions associated with hormone, light,and circadian rhythm signaling, suggesting that the CBFs act as key integrators of endogenous and external environmental cues. Our findings not only define the genome-wide binding patterns of the CBFs during the early cold response, but also provide insights into the role of the CBFs in regulating multiple biological processes of plants. | Yue Song Xiaoyan Zhang Minze Li Hao Yang Diyi Fu Jian Lv Yanglin Ding Zhizhong Gong Yiting Shi Shuhua Yang | 2021 | Journal of Integrative Plant Biology2021，63，11： | 3 |
| 4 | 水曲柳(Fraxinus mandshurica)COR413基因的克隆及表达模式分析显示文摘冷调节基因(cold-regulating gene,COR基因)对植物受低温胁迫后提升其低温耐受性起重要作用。本研究以水曲柳为材料,克隆得到一个COR基因,序列比对后,命名为FmCOR413,编码区全长631 bp,共编码209个氨基酸。生物信息学分析可知其含有一个WCOR413保守结构域,属于WCOR413超家族中的一员。同源比对结果显示,FmCOR413基因碱基序列与15个物种序列同源性在95%~77%之间,与油橄榄的同源性最高为95%。荧光定量PCR显示该基因的表达具有节律性;在低温胁迫后,节律性被打破,基因表达量相对于常温有显著提高,通过分析可知COR413的节律性与植物低温耐受性有很大关联。测定4℃低温处理的水曲柳叶片的叶绿素含量、丙二醛(malondialdehyde,MDA)含量、超氧化物歧化酶(superoxide dismutase,SOD)活性、过氧化物酶(peroxidase,POD)活性,得到叶绿素含量降低3.9%、MDA含量升高5.8%、SOD活性升高7.4%、POD活性升高75.5%的结果。研究结果为探明该基因在水曲柳响应低温胁迫的过程中的功能和机制提供了参考,同时为水曲柳低温耐受性的分子改良提供了依据。 | 王鹏洋 韩子昂 翟玉欢 赵晓航 曹羊 詹亚光 | 2019 | 分子植物育种2019，17，24： | 2 |
| 5 | 植物抗寒基因及ICE-CBF-COR信号转导研究进展显示文摘包括冷胁迫和冻胁迫在内的低温逆境胁迫是造成农作物减产的主要因素之一。研究转录因子CBF调节的植物低温应答途径对于揭示植物面对低温胁迫时的分子反应有重要意义。针对植物抗寒基因及ICE-CBF-COR信号通路的研究,从植物抗寒基因、信号通路和激素与组蛋白调控等多个方面对当下植物抗寒基因的研究进展进行了综述,表明ICE-CBFCOR是不同转录因子、调控因子、蛋白质、生理因子和其他操纵因子相互作用以增强冷胁迫的中心途径;同时也发现ICE-CBF-COR信号通路在针对植物生长发育、花期调节、保鲜贮藏和遗传育种等相关研究中同样起着至关重要的作用,并对未来研究与发展的前景进行了展望,以期为植物抗寒基因的研究提供参考与理论支持,同时也为植物的生长发育、花期调节、保鲜贮藏和遗传育种提供理论指导。 | 田一粟 姜明 张启宇 | 2023 | 农业工程2023，13，5： | 0 |
| 6 | Transcriptional regulation of bark freezing tolerance in apple(Malus domestica Borkh.)显示文摘Freezing tolerance is a significant trait in plants that grow in cold environments and survive through the winter.Apple(Malus domestica Borkh.)is a cold-tolerant fruit tree,and the cold tolerance of its bark is important for its survival at low temperatures.However,little is known about the gene activity related to its freezing tolerance.To better understand the gene expression and regulation properties of freezing tolerance in dormant apple trees,we analyzed the transcriptomic divergences in the bark from 1-year-old branches of two apple cultivars,“Golden Delicious”(G)and“Jinhong”(H),which have different levels of cold resistance,under chilling and freezing treatments.“H”can safely overwinter below−30℃in extremely low-temperature regions,whereas“G”experiences severe freezing damage and death in similar environments.Based on 28 bark transcriptomes(from the epidermis,phloem,and cambium)from 1-year-old branches under seven temperature treatments(from 4 to−29°C),we identified 4173 and 7734 differentially expressed genes(DEGs)in“G”and“H”,respectively,between the chilling and freezing treatments.A gene coexpression network was constructed according to this expression information using weighted gene correlation network analysis(WGCNA),and seven biologically meaningful coexpression modules were identified from the network.The expression profiles of the genes from these modules suggested the gene regulatory pathways that are responsible for the chilling and freezing stress responses of“G”and/or“H.”Module 7 was probably related to freezing acclimation and freezing damage in“H”at the lower temperatures.This module contained more interconnected hub transcription factors(TFs)and cold-responsive genes(CORs).Modules 6 and 7 contained C-repeat binding factor(CBF)TFs,and many CBF-dependent homologs were identified as hub genes.We also found that some hub TFs had higher intramodular connectivity(KME)and gene significance(GS)than CBFs.Specifically,most hub TFs in modules 6 and 7 were activated at the beginning of the early freezing stress phase and maintained upregulated expression during the whole freezing stress period in“G”and“H”.The upregulation of DEGs related to methionine and carbohydrate biosynthetic processes in“H”under more severe freezing stress supported the maintenance of homeostasis in the cellular membrane.This study improves our understanding of the transcriptional regulation patterns underlying freezing tolerance in the bark of apple branches. | Yinghai Liang Shanshan Wang Chenhui Zhao Xinwei Ma Yiyong Zhao Jing Shao Yuebo Li Honglian Li Hongwei Song Hong Ma Hao Li Bingbing Zhang Liangsheng Zhang | 2020 | Horticulture Research2020，7，1： | 0 |
| 7 | 拟南芥膜定位蛋白At CP2调控抗冻性的功能分析显示文摘COR家族成员在调控植物响应低温胁迫过程中发挥重要作用。从拟南芥中克隆一个新的低温胁迫响应基因At CP2,其属于COR基因家族中的COR413亚家族,编码区612 bp,编码203个氨基酸,分子量22.75k Da,等电点9.44。亚细胞定位和基因表达模式分析结果表明:At CP2定位在叶绿体膜上,对ABA、PEG、Na Cl和低温四种非生物胁迫均有响应。基因功能分析结果显示:功能缺失性突变体cp2冷处理后存活率明显高于野生型拟南芥(WT),相对电导率和MAD均低于WT,证明At CP2基因负向调控植物抗冻性。蛋白质组及qRT-PCR分析结果证明At CP2通过负向调控4个ABA及低温胁迫相关基因(包括ABA2、KIN1、COR47和COR15B)的表达影响植物抗冻性。总之,At CP2基因的功能分析结果对了解COR413基因家族的功能提供了参考。 | 齐欣 丁庆倩 苏晨 徐伟亚 牟永莹 葛林豪 徐兆师 陈隽 潘映红 马有志 陈明 | 2019 | 中国农业科技导报2019，21，9： | 0 |
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