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| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | Molecular chaperone functions of heat shock proteins显示文摘 | Hendrick J P Hartl F U | 1993 | Annu Rev Biochem1993，62，： | 2 |
| 3 | Soil test phosphorus and clay content effects on runoff water quality显示文摘 | Cox F A Hendricks S E | 2000 | Journal of Environmental Quality2000，29，： | 1 |
| 4 | Molecular chaperone functions of the heat-shock proteins显示文摘 | Hendrick J P Hartl F U | 1993 | Annu Rev Biochem1993，62，34： | 1 |
| 5 | Assessment by transcription-mediated amplification,of virologic response in patients with chronic hepatitis C virus treated with peginterferon alpha-2a 显示文摘 | SARRAZIN C HENDRICKS DA SEDARATI F | 2001 | J Clin Microbiol2001，39，： | 1 |
| 6 | High aspect ratio microstructuring of transparent dielectrics using femtosecond laserpulses: method for optimization of the machining throughput显示文摘 | Hendricks F der Au J A Matylitsky V V | 2014 | Appl Phys A2014，117，1： | 1 |
| 7 | Molecular chaperone functions of heat-shock proteins显示文摘 | Hendrick J P Hartl F | 1993 | Annual Review of Biochemistry1993，62，： | 1 |
| 8 | An intercomparison campaign of ground-based UV-visible measurements of NO2,BrO,and OClO slant columns:Methods of analysis and results for NO2显示文摘 | Vandaele A C Fayt C Hendrick F | | 0，，： | 1 |
| 9 | Prescribing practices of primarcare veterinary practitioners in dogs diagnosed with bacterial pyoderma显示文摘 | Summers J F Hendricks A Brodbelt D C | 2014 | BMC Vet Res2014，10，： | 1 |
| 10 | Rapid chemotaxonomy of pathogenic bacteria using in situ thermal hydrolysis and methylation as a sample prepration step coupled with a field-portable membrane-inlet quadrupole ion trap mass spectrometer显示文摘 | Hendricker AD Christy AH Basile F | 1999 | Int J Mass Spectrum1999，，： | 1 |
| 11 | Molecular Chaperone Function of Heat - shock Proteins显示文摘 | Hendrick J P Hartl F U | 1993 | Annu Rev Biochem1993，，62： | 1 |
| 12 | Assessing large-sce weekly cycles in meteorological variables: A review 显示文摘 | Sanchez-Lorenzo A Laux P Hendricks F | 2012 | Atmospheric Chemistry and Physics2012，12，13： | 1 |
| 13 | The economics of hubs:The case of monopoly显示文摘 | Hendricks K Piccione M Tan G F | 1995 | The Review of Economic Studies1995，62，1： | 1 |
| 14 | A Numeri- cal-experimental Method to Characterize the Non-linear Mechanical Be- havior of Human Skin显示文摘 | HENDRICKS F M BROKKEN D OOMENS C W J | 2003 | Skin Research & Technology2003，9，3： | 1 |
| 15 | Molecdar chaperone functions of heat-shock proteins显示文摘 | HENDRICK J P HARTL F U | 1993 | Annu Rev Biochem1993，72，： | 1 |
| 16 | Molecular chaperone functions of heat-shock proteins显示文摘 | HENDRICK J P HARTL F U | 1993 | Annual Review of Biochemistry1993，，62： | 1 |
| 17 | Retrieval of stratospheric and tropospheric BrO columns from multi-axis DOAS measurements at Reunion Island ( 21° S, 56° E ) 显示文摘 | Theys N Van Roozendael M Hendrick F | 2007 | Atmospherical Chemistry and Physics2007，，7： | 1 |
| 18 | The role of molecular chaperones in protein folding显示文摘 | Hendrick J P Hartl F U | 1995 | FASEB J1995，9，15： | 1 |
| 19 | Molecular chaperone function of heat shock proteins显示文摘 | HENDRICK J P HARIL F | 1993 | Ann Rev Biochem1993，62，： | 1 |
| 20 | The influence of carbon fiber belts and composites by pyrolytic carbon deposi- tion from propylene 显示文摘 | McAllister P Hendricks J F Wolf E E | 1990 | Carbon1990，28，4： | 1 |
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