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| 1 | Plasmacytoid dendritic cell deficiency in neonates enhances allergic airway inflammation via reduced production of IFN-α显示文摘Allergic asthma,a chronic inflammatory airway disease associated with type 2 cytokines,often originates in early life.Immune responses at an early age exhibit a Th2 cell bias,but the precise mechanisms remain elusive.Plasmacytoid dendritic cells(pDCs),which play a regulatory role in allergic asthma,were shown to be deficient in neonatal mice.We report here that this pDC deficiency renders neonatal mice more susceptible to severe allergic airway inflammation than adult mice in an OVA-induced experimental asthma model.Adoptive transfer of pDCs or administration of IFN-αto neonatal mice prevented the development of allergic inflammation in wild type but not in IFNAR1−/−mice.Similarly,adult mice developed more severe allergic inflammation when pDCs were depleted.The protective effects of pDCs were mediated by the pDC-/IFN-α-mediated negative regulation of the secretion of epithelial cell-derived CCL20,GM-CSF,and IL-33,which in turn impaired the recruitment of cDC2 and ILC2 cells to the airway.In asthmatic patients,the percentage of pDCs and the level of IFN-αwere lower in children than in adults.These results indicate that impairment of pDC-epithelial cell crosstalk in neonates is a susceptibility factor for the development of allergeninduced allergic airway inflammation. | Min Wu Liuchuang Gao Miao He Hangyu Liu Han Jiang Ketai Shi Runshi Shang Bing Liu Shan Gao Hebin Chen Feili Gong Erwin WGelfand Yafei Huang Junyan Han | 2020 | Cellular & Molecular Immunology2020，17，5： | 3 |
| 2 | A 3D bio-printed spheroids based perfusion in vitro liver on chip for drug toxicity assays显示文摘Liver is the foremost organ of human being for drug metabolism,and it played a significant role in toxicity evaluation of drugs.Establishing a liver model in vitro can accelerate the process of the drug screening and new drug research and development.We provide a 3D printing based hepatic sinusoid-on-a-chip microdevice that reconstitutes organ-level liver functions to create a drug screening model of toxicity evaluation on chip.The microfluidic device,which recapitulates the hepatic sinusoid microenvironment,consists of PET polyporous membranes which mimic the perisinusoidal space,and experience fluid flow to mimic the hepatic arterial capillaries.The PET membrane was used to separate the hepatocyte and endotheliocyte.The endotheliocyte was cultured on the downside of the membrane and the hepatocyte were 3D seeded on the membrane via the 3D printer.This device was used to reproduce the in vitro liver model for drug toxicity assays.The expression of several biomarkers of liver was compared with the monoculture and 2D cultured conditions,and the results reveal that this organ-on-a-chip microdevice mimics the drug hepatoxicity that has not been possible by 2D cell-based and animal models,providing a useful platform for screening the drugs and developing an effective therapy in hepatopathy. | Tian Tian Yuhan Ho Chen Chen Hebin Sun Janan Hui Panhui Yang Yuqing Ge Ting Liu Jian Yang Hongju Mao | 2022 | Chinese Chemical Letters2022，33，6： | 1 |
| 3 | Exploration and Practice on the Construction Mode of Biopharmaceutical Training Base in Application-based Colleges and Universities显示文摘By using the methods of literature analysis,field investigation,exploration and research,experience summary,comparative inductive analysis,etc.,through the search of the latest literature and policy materials,production enterprises and domestic application-based undergraduate universities with related majors have been investigated on the spot.By analyzing the failed cases and drawing lessons from the successful experience,this paper explores the transformation and practical construction of the biopharmaceutical training base in application-based colleges and universities.This paper innovates and designs a new construction mode of biopharmaceutical training base in application-based universities,which provides reliable experience and valuable research results for the construction or transformation of biopharmaceutical training base in application-based colleges and universities.It has a very important potential value for promoting the social and economic benefits. | Zhongwei CHEN Hebin LI Ying ZENG Qihuang LIN Ruonan HE Xuan HONG | 2021 | Medicinal Plant2021，12，2： | 0 |
| 4 | Occurrence and Removal of Antibiotic Resistance in Nationwide Hospital Wastewater Deciphered by Metagenomics Approach-China,2018-2022显示文摘Summary What is already known about this topic?Hospital wastewater represents a significant conduit for the dissemination of antibiotic resistance genes(ARGs),including those that are of clinical importance.Yet,the variation in antibiotic resistance on a national level,which can be influenced by regional differences,has not been thoroughly elucidated. | Jin Huang Zhongyu Wang Zihan Chen Hebin Liang Xiaoyan Li Bing Li | 2023 | China CDC weekly2023，5，46： | 0 |
| 5 | Substitution of His260 residue alters the thermostability of Pseudoalteromonas carrageenovora arylsulfatase显示文摘This study aimed to improve the thermostability of arylsulfatase from Pseudoalteromonas carrageenovora. A library of P. carrageenovora arylsulfatase mutants was constructed by introducing random mutagenesis using error-prone PCR. After screening, two mutants of H260L and D84A/H260L showed enhanced thermal stability than the wild-type predecessor (WT). Site-directed mutagenesis demonstrated that only amino acid residue at Position 260 plays an important role in the thermostability of P. carrageenovora arylsulfatase. Thermal inactivation analysis showed that the half-life (t1/2) values at 55°C for H260L, H260I, H260Q, H260F and H260R were 40.6, 48.4, 30.9, 29.1 and 34.5 min, respectively, while that of WT was 9.1 min. Structure modeling demonstrated that the additional hydrogen bonds and/or optimization of surface charge-charge interactions could be responsible for the increased thermostability imparted by H260L, H260I, H260Q, H260F and H260R. | Yanbing Zhu Xiaoqian Yin Han Liu Hebin Li Yanhong Chen Lijun Li Anfeng Xiao Hui Ni | 2019 | Acta Oceanologica Sinica2019，38，6： | 0 |
| 6 | Energy Supplies Ensured in Earthquake Region显示文摘China is releasing oil products from its state reserve stockpiles to areas stricken by earthquake on May 12 to ensure energy supply. PetroChina has also | Chen Hebin | 2008 | China Oil & Gas2008，15，2： | 0 |
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