| 1 | A small scale expression screen identifies tissue specific markers in the Dugesia japonica strain Pek-1显示文摘Freshwater planaria has tremendous capacity to reform the missing part of the body and therefore is considered as one of the most important model organism for regeneration study. At present, Schmidtea mediterranea and Dugesia japonica are the two major species utilized for laboratory manipulations. Dugesia japonica flatworms are widely distributed in the Far East including Cherry Valley region in the northwest area of Beijing, China. We reported here the establishment of an asexual Dugesia japonica strain Pek1, as a suitable system for regeneration study. Using morphological, karyotypical as well as phylogenetic analyses, we confirmed that these flatworms indeed belonged to Dugesia japonica. We went on to show that the commonly used in situ probes and immunohistochemistry reagents and protocols were applicable to the Pek1 strain. Using this strain, we carried out small scale analysis on EST, RNAi and gene expression. We identified 193 unique EST sequences and 65 of them had not been reported in planarian. By RNAi analysis, we showed that 48 genes, when downregulated individually, had no effect on regeneration. Furthermore, we identified 3 groups of tissue specific expressing genes that were useful for cell lineage analysis. We concluded that the Dugesia japonica Pek1 strain could be another suitable animal model to regeneration research. | Changxin Ma Xiaoan Wang Shuizhou Yu Guoliang Chai Hanxia Su Liang Zheng Wei Wu | 2010 | Journal of Genetics and Genomics2010，37，9： | 3 |
| 2 | The invasive potential of a hybrid species: insights from soil chemical properties and soil microbial communities显示文摘作为适应性进化,入侵物种与本土物种的自然杂交威胁着全球生物多样性。然而,杂交种的潜在入侵机制尚未得到系统研究,尤其是在土壤化学性质和土壤微生物群落方面。在一个为期两年的田间试验中,对入侵种南美蟛蜞菊(Sphagneticola trilobata)和杂交蟛蜞菊(南美蟛蜞菊与本地蟛蜞菊S.calendulacea杂交)的土壤微生物群落、土壤解钾菌和解磷菌菌落数、土壤酶活性以及叶片光饱和速率进行了测定。结果表明:杂交蟛蜞菊土壤可溶性有机碳和解磷菌菌落数显著高于南美蟛蜞菊和本地蟛蜞菊。土壤酸性磷酸酶、总PLFA、细菌PLFA、真菌PLFA、纤维素酶、脲酶在三种处理间无显著差异。杂交蟛蜞菊光饱和光合速率、光合氮、磷和钾利用效率显著高于入侵种南美蟛蜞菊,但与本地蟛蜞菊无显著差异。杂交蟛蜞菊总生物量和根生物量显著高于本地蟛蜞菊。因此,与本地蟛蜞菊相比,杂交蟛蜞菊仍然有较高的入侵性,其未来可能加剧本地蟛蜞菊的灭绝。 | Feng Sun Yuyi Ou Qiaojing Ou Lingda Zeng Hanxia Yu Jin Zheng Lei Gao Weihua Li Na Li Changlian Peng | 2020 | Journal of Plant Ecology2020，13，1： | 0 |
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