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| 1 | Combined Applications of Nitrogen and Phosphorus Fertilizers with Manure Increase Maize Yield and Nutrient Uptake via Stimulating Root Growth in a Long-Term Experiment显示文摘Imbalanced application of nitrogen(N) and phosphorus(P) fertilizers can result in reduced crop yield,low nutrient use efficiency,and high loss of nutrients and soil nitrate nitrogen(NO_3^--N) accumulation decreases when N is applied with P and/or manure;however,the effect of applications of N with P and/or manure on root growth and distribution in the soil profile is not fully understood.The aim of this study was to investigate the combined effects of different N and P fertilizer application rates with or without manure on maize(Zea mays L.) yield,N uptake,root growth,apparent N surplus,Olsen-P concentration,and mineral N(N_(min)) accumulation in a fluvo-aquic calcareous soil from a long-term(28-year) experiment.The experiment comprised twelve combinations of chemical N and P fertilizers,either with or without chicken manure,as treatments in four replicates.The yield of maize grain was 82%higher,the N uptake 100%higher,and the N_(min) accumulation 39%lower in the treatments with combined N and P in comparison to N fertilizer only.The maize root length density in the 30-60 cm layer was three times greater in the treatments with N and P fertilizers than with N fertilizer only.Manure addition increased maize yield by 50%and N uptake by 43%,and reduced N_(min)(mostly NO_3^--N) accumulation in the soil by 46%.The long-term application of manure and P fertilizer resulted in significant increases in soil Olsen-P concentration when no N fertilizer was applied.Manure application reduced the apparent N surplus for all treatments.These results suggest that combined N and P fertilizer applications could enhance maize grain yield and nutrient uptake via stimulating root growth,leading to reduced accumulation of potentially leachable NO_3^--N in soil,and manure application was a practical way to improve degraded soils in China and the rest of the world. | WEN Zhihui SHEN Jianbo Martin BLACKWELL LI Haigang ZHAO Bingqiang YUAN Huimin | 2016 | Pedosphere2016，26，1： | 29 |
| 2 | Inhibition of FOXO3a/BIM signaling pathway contributes to the protective effect of salvianolic acid A against cerebral ischemia/reperfusion injury显示文摘Salvianolic acid A(SalA) is an effective compound extracted from traditional Chinese medicine Salvia miltiorrhiza Bunge. The Forkhead box O3a(FOXO3a) signaling pathway plays crucial roles in the modulation of ischemia-induced cell apoptosis. However, no information about the regulatory effect of SalA on FoxO3a is available. To explore the anti-cerebral ischemia effect and clarify the therapeutic mechanism of SalA, SH-SY5Y cells and Sprague–Dawley rats were applied, which were exposed to oxygen glucose deprivation/reoxygenation(OGD/R) and middle cerebral artery occlusion/reperfusion(MCAO/R) injuries, respectively. The involved pathway was identified using the specific inhibitor LY294002. Results showed that SalA concentration-dependently inhibited OGD/R injury triggered cell viability loss. SalA reduced cerebral infarction, lowered brain edema, improved neurological function, and inhibited neuron apoptosis in MCAO/R rats, which were attenuated by the treatment of phosphatidylinositol-4,5-bisphosphate 3-kinase(PI3K) specific inhibitor LY294002. SalA time-and concentration-dependently upregulated the phosphorylation levels of protein kinase B(AKT) and its downstream protein FOXO3a. Moreover, the nuclear translocation of FOXO3a was inhibited by SalA both in vivo and in vitro, which was also reversed by LY294002. The above results indicated that SalA fought against ischemia/reperfusion damage at least partially via the AKT/FOXO3a/BIM pathway. | Junke Song Wen Zhang Jinhua Wang Haiguang Yang Qimeng Zhou Haigang Wang Li Li Guanhua Du | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 20 |
| 3 | An overview of the use of plastic-film mulching in China to increase crop yield and water-use efficiency显示文摘Since 1990,crop yields have stagnated in many parts of the world,posing a significant challenge to global food security[1].As such;innovative solutions are needed to increase the resource-use efficiency of cropping systems to produce more grains per unit area. | Dongbao Sun Haigang Li Enli Wang Wenqing He Weiping Hao Changrong Yan Yuzhong Li Xurong Mei Yanqing Zhang Zhanxiang Sun Zhikuan Jia Huaiping Zhou Tinglu Fan Xucheng Zhang Qin Liu Fengju Wang Chaochun Zhang Jianbo Shen Qingsuo Wang Fusuo Zhang | 2020 | National Science Review2020，7，10： | 19 |
| 4 | Waxy allelic diversity in common millet(Panicum miliaceum L.) in China显示文摘A set of 132 accessions of common millet,Panicum miliaceum L.,from 12 provinces of China were assessed for endosperm starch type(waxy or non-waxy)using I_2-KI staining,amylose and amylopectin contents using the dual-wavelength colorimetric method,and genotype of Waxy genes conditioning amylose content by gene sequencing.Endosperm starch content varied from57.69%to 74.70%,while the amylose and amylopectin contents of the starch ranged from 0 to23.29%and from 41.99%to 70.24%,respectively.Sequencing two Wx genes,including Wx-L(intron 5-exon 7 and intron 8–9)and Wx-S(exon 9–intron 10)revealed several polymorphisms(S_0,S_(-15),L_C,L_F,L_Y).Marker M5-R11 linking to the Wx-S gene may be used to discriminate waxy common millet accessions from non-waxy ones.Among the 132 accessions,68 with the S_(-15)allele had waxy endosperm starch with the amylose content range 0–2.58%and 64 accessions with the S_0allele had non-waxy endosperm starch with amylose content range 3.94%–23.29%.Five genotypes including S_(-15)/L_F(45%of the accessions),S_0/L_F(25%),S_0/L_Y(12%),S_0/L_C(11%),and S_(-15)/L_Y(7%)were identified.Six new SNPs were detected at the Wx-L locus.These results will facilitate common millet breeding,especially of cultivars free of amylose. | Ruiyun Wang Haigang Wang Xiaohuan Liu Xu Ji Ling Chen Ping Lu Minxuan Liu Bin Teng Zhijun Qiao | 2018 | The Crop Journal2018，6，4： | 10 |
| 5 | Applications of Electrical Capacitance Tomography in Two Phase Flow Visualization显示文摘The successful application of Electrical Capacitance Tomography (ECT) depends heavily on the image reconstruction speed and quality. The two requirements usually cannot be satisfied simultaneously. Also, for a large number of acquired 2D ECT images, a 3D presentation of them is much desired to track the variations of the material distribution in a third dimension. To facilitate ECT for practical flow analyses in process engineering, the authors have recently developed algorithms for 3D image presentation and for online iterative image reconstruction that only takes the same time as a linear back projection method but yields good image qualities as the Landweber iterative method does. The new methods have been successfully applied to visualize several classes of two phase flows, namely fluidization, cyclone separation, circulating rate determination in circulating fluidized beds, pneumatic conveying, rotating drum mixing. Special characteristics in each case have been discussed and valuable results are obtained, which are reported in this paper. | Shi Liu Runsheng Yan Haigang Wang Fan Jiang Xiangyun Dong | 2004 | Journal of Thermal Science2004，13，2： | 9 |
| 6 | Pathogen-inducible OsMPKKI 0.2-OsMPK6 cascade phosphorylates the Raf-like kinase OsEDRI and inhibits its scaffold function to promote rice disease resistance显示文摘Mitogen-activated protein kinase(MAPK)cascades regulate a myriad of plant biological processes,including disease resistance.Plant genomes encode a large number of MAPK kinase kinases(MAPKKKs)that can be divided into two subfamilies,namely MEKK-like kinases and Raf-like kinases.Thus far,the func-tions of MEKK-like MAPKKKs have been relatively well characterized,but the roles of Raf-like MAPKKKs in.plant MAPK cascades remain less understood.Here,we report the role of OsEDR1,a Raf-like MAPKKK,in the regulation of the MAPK cascade in rice response tp the bacterial pathogen Xanthomonas oryzae pv.oryzicola(Xoc).We found that OsEDR1 inhibits OsMPKK10.2(a MAPK kinase)activity through physical interaction.Upon Xoc infection,OsMPKK10.2 is phosphorylated at S304 to activate OsMPK6(a MAPK).Interestingly,activated OsMPK6 phosphorylates OsEDR1 at S861,which destabilizes OsEDR1 and thus releases the inhibition of OsMPKK10.2,leading to increased OsMPKK10.2 activity and enhanced resistance of rice plants to Xoc.Taken together,these results provide new insights into the functions of Raf-like ki-nases in the regulation of the MAPK cascade in plant immunity. | Haigang Ma Juan Li Ling Ma Peilun Wang Yuan Xue Ping Yin Jinghua Xiao Shiping Wang | 2021 | Molecular Plant2021，14，4： | 9 |
| 7 | Structures and catalytic performances of Me/SAPO-34(Me=Mn,Ni,Co)catalysts for low-tem perature SCR of NO_x by ammonia显示文摘Me/SAPO-34(Me=Mn,Ni,Co)series of catalysts were prepared by a wetness impregnation method and investigated for the selective catalytic reduction of nitrogen oxides with ammonia(NH 3-SCR).Among them,Mn/SAPO-34 catalyst was found as the most promising candidate based on its superior low-temperature activity.The catalysts were characterized by X-ray diffraction(XRD),transmission electron microscopy images(TEM),nuclear magnetic resonance(NMR),X-ray photoelectron spectroscopy(XPS),temperature programmed reduction and desorption(TPR and TPD),and diffuse reflectance infrared Fourier transformed spectroscopy(DRIFTS)of NH_(3)/NO_(x)adsorption.Mn/SAPO-34 is obviously different from Ni/SAPO-34 and Co/SAPO-34 in the active species state and distribution.Surface MnO_(x)species which play an essential role in NO oxidation and NO_(2)adsorption,act as better active sites than nickel and cobalt mostly in the form of the aluminates and silicates. | Xuesong Liu Zhiming Sui Hongfeng Chen Yong Chen Haigang Liu Peng Jiang Zhongyun Shen Wensheng Linghu Xiaodong Wu | 2021 | Journal of Environmental Sciences2021，33，6： | 8 |
| 8 | Influence of the intensity and loading time of direct current electric field on the directional migration of rat bone marrow mesenchymal stem cells显示文摘Exogenic 电场能有效地加速骨头愈合并且向受伤区域通过骨头髓的提高的迁居改变间充质的干细胞(BMSC ) 。这研究试图决定下列:(1 ) 在到一个直接当前的电场(DCEF ) 的暴露之上的老鼠 BMSC (rBMSC ) 移植的方向,(2 ) 最佳的 DCEF 紧张和持续时间,并且(3 ) 在由 DCEF 导致了的 rBMSC 移植的 SDF-1/CXCR4 轴的可能的规章的角色。显示出的结果 rBMSCs 移居了到 DCEF 的积极电极,并且为 4 h 的 200 mV/mm 的 DCEF 被发现在提高 rBMSC 移植最佳。这 DCEF 力量和持续时间另外 upregulated osteoblastic 基因,包括的高山和 OCN 的表示,和 upregulated 高山和 Runx2 蛋白质的表示。而且当 CXCR4 被禁止时, rBMSC 移植部分由于 DCEF 被堵住。这些调查结果显示 DCEF 能有效地导致 rBMSC 移植。因为它支持 rBMSC 移植,增长,和 osteogenic 区别的能力,为 4 h 的 200 mV/mm 的 DCEF 被推荐。表明小径的 SDF-1/CXCR4 可以在调整 rBMSCs 的导致 DCEF 的迁居起一个重要作用。 | Xiaoyu Wang Yuxuan Gao Haigang Shi Na Liu Wei Zhang Hongbo Li | 2016 | Frontiers of Medicine2016，10，3： | 7 |
| 9 | Object-level change detection based on full-scale image segmentation and its application to Wenchuan Earthquake显示文摘Aiming at object fragmentation and poor detection results caused by discontinuous segmentation scale in object-level change detection,a new object-level change detection method based on the full-scale object tree is presented in this paper.The core idea of this new algorithm is to establish the full-scale object tree based on convexity model theory and integrate full-scale image segmentation techniques and change detection into the whole process.Some Wenchuan Earthquake images are taken as an example to discuss the new method for earthquake damage detection and evaluation in urban area,landslide detection,and extraction of barrier lake boundary.The application shows that the new method is robust and it provides an advanced tool for the quantitative detection and evaluation of earthquake damage. | GONG JianYa,SUI HaiGang,SUN KaiMin,MA GuoRui & LIU JunYi State Key Laboratory of Information Engineering in Surveying,Mapping and Remote Sensing,Wuhan University,Wuhan 430072,China | 2008 | Science China(Technological Sciences)2008，51，S2： | 7 |
| 10 | REVIEW OF ADVANCED FPGA ARCHITECTURES AND TECHNOLOGIES显示文摘Field Programmable Gate Array(FPGA) is an efficient reconfigurable integrated circuit platform and has become a core signal processing microchip device of digital systems over the last decade. With the rapid development of semiconductor technology, the performance and system integration of FPGA devices have been significantly progressed, and at the same time new challenges arise. The design of FPGA architecture is required to evolve to meet these challenges, while also taking advantage of ever increased microchip density. This survey reviews the recent development of advanced FPGA architectures, including improvement of the programming technologies, logic blocks, interconnects, and embedded resources. Moreover, some important emerging design issues of FPGA architectures, such as novel memory based FPGAs and 3D FPGAs, are also presented to provide an outlook for future FPGA development. | Yang Haigang Zhang Jia Sun Jiabin Yu Le | 2014 | Journal of Electronics(China)2014，31，5： | 6 |
| 11 | Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner显示文摘Autophagy is an evolutionarily-conserved selfdegradative process that maintains cellular homeostasis by eliminating protein aggregates and damaged organelles.Recently, vesicle-associated membrane protein-associated protein B(VAPB), which is associated with the familial form of amyotrophic lateral sclerosis, has been shown to regulate autophagy. In the present study, we demonstrated that knockdown of VAPB induced the up-regulation of beclin 1 expression, which promoted LC3(microtubuleassociated protein light chain 3) conversion and the formation of LC3 puncta, whereas overexpression of VAPB inhibited these processes. The regulation of beclin1 by VAPB was at the transcriptional level. Moreover,knockdown of VAPB increased autophagic flux, which promoted the degradation of the autophagy substrate p62 and neurodegenerative disease proteins. Our study provides evidence that the regulation of autophagy by VAPB is associated with the autophagy-initiating factor beclin 1. | Dan Wu Zongbing Hao Haigang Ren Guanghui Wang | 2018 | Neuroscience Bulletin2018，34，6： | 6 |
| 12 | Familial Parkinson's Disease-Associated L166P Mutant DJ-1 is Cleaved by Mitochondrial Serine Protease Omi/HtrA2显示文摘Parkinson's disease(PD) is the most common neurodegenerative movement disorder. Mutations in the DJ-1, including L166 P, are responsible for recessive earlyonset PD. Many lines of evidence have shown that L166 P is not only a loss-of-function mutant, but also a proapoptotic-like protein that results in mitochondrial dysfunction. L166 P has been reported to be unstable and to mislocalize to mitochondria. However, the mechanisms underlying the instability of L166 P compared to wild-type DJ-1 remain largely unknown. Here, we showed that Omi/Htr A2, a mitochondrial serine protease that has also been linked to the pathogenesis of PD, contributed to L166 P instability. Omi directly interacted with and cleaved L166 P in mitochondria to decrease the L166 P level. However,Omi did not bind and cleave wild-type DJ-1. Moreover,Omi cleaved L166 P at both serine residues 3 and 121,while L166 P-induced cell death under H_2O_2 treatment was alleviated by over-expression of Omi. Our data reveal a bridge between DJ-1 and Omi, two PD-associated geneticfactors, which contributes to our understanding of the pathogenesis of PD. | Kai Fu Yanfei Wang Dongkai Guo Guanghui Wang Haigang Ren | 2017 | Neuroscience Bulletin2017，33，6： | 6 |
| 13 | Ni(OH)_(2) nanoparticles encapsulated in conductive nanowire array for high-performance alkaline seawater oxidation显示文摘Design and development of high-efficiency and durable oxygen evolution reaction(OER)electrocatalysts is crucial for hydrogen production from seawater splitting.Herein,we report the in situ electrochemical conversion of a nanoarray of Ni(TCNQ)2(TCNQ=tetracyanoquinodimethane)on graphite paper into Ni(OH)_(2) nanoparticles confined in a conductive TCNQ nanoarray(Ni(OH)_(2)-TCNQ/GP)by anode oxidation.The Ni(OH)_(2)-TCNQ/GP exhibits high OER performance and demands overpotentials of 340 and 382 mV to deliver 100 mA·cm^(−2) in alkaline freshwater and alkaline seawater,respectively.Meanwhile,the Ni(OH)_(2)-TCNQ/GP also demonstrates steady long-term electrochemical durability for at least 80 h under alkaline seawater. | Longcheng Zhang Jiaqian Wang Pengyu Liu Jie Liang Yongsong Luo Guanwei Cui Bo Tang Qian Liu Xuedong Yan Haigang Hao Meiling Liu Rui Gao Xuping Sun | 2022 | Nano Research2022，15，7： | 5 |
| 14 | An energy-conserving two-way coupled mode model for underwater acoustic propagation显示文摘In order to avoid the energy non-conservation in two-way sound field calculations,which results from certain approximation of non-horizontal boundary,an energy-conserving twoway coupled mode propagation model is developed.During the derivation of this model,the non-horizontal boundary condition is treated strictly instead of making an approximation.The coupling equations,which are easy to numerical implementation,are derived from the equation of motion and the equation of continuity.Moreover,the validity of this model is illustrated with several numerical examples of different bottom parameters and the equation of energy conservation.It is shown that a boundary correction is introduced by the strict formulizing of the boundary condition in this model.This boundary correction guarantees the coupling coefficients being symmetrical and makes the sound fields satisfy the energy conservation.Thus,the energy-conserving coupled model is able to handle non-horizontal boundaries adequately,and the two-way sound fields in a non-horizontal stratified media can be calculated exactly by this model. | MO Yaxiao PIAO Shengchun ZHANG Haigang LI Li | 2016 | Chinese Journal of Acoustics2016，35，2： | 5 |
| 15 | Experimental Study on Measurement of Flame Temperature Distribution Using the Two-Color Method显示文摘A novel optical instrumentation system for flame characterization was developed and tested. The two-color system is capable of performing on-line measurement of flame and temperature distribution, providing temporal and spatial characterization of the combustion process. In addition, the system has the advantage of being non-intrusive. The on-line measurement of temperature distribution on a methane-air combustor revealed some interesting characteristics. When air flow rate was kept constant, an increase in CH4 flow rate would enhance the combustion intensity and elevate the flame temperature. While under constant CH4 flow rate, the increase of air flow rate would lead the measured flame temperature first to increase and then decrease. The drop of temperature might partially be caused by the heat losses due to excessive air. However, with insufficient soot particles in the flame, the temperature could be under-evaluated. | Fan Jiang Shi Liu Gang Lu Yong Yan Haigang Wang Yanmin Song Renxiong Ma Zhonggang Pan | 2002 | Journal of Thermal Science2002，11，4： | 5 |
| 16 | Investigation of gas-solids flow hydrodynamics in a cold model of a dual fluidised bed gasifier using electrical capacitance tomography sensors显示文摘The flow characteristics of a dual fluidised bed gasifier(DFBG)are more complex than those of a single fluidised bed gasifier.For stable operation and appropriate control,a cold DFBG test facility with both an upper and a lower U-valve was built,and electrical capacitance tomography(ECT)sensors were installed with pressure transducers to investigate the effects of operating conditions on gas-solids flow hydrodynamics.The operating parameters included gas velocities in the riser and in the bubbling fluidised bed,aeration velocity in the lower U-valve,bed material inventory,and particle size.This is the first time that ECT was applied in different flow zones of a dual fluidised bed gasifier system.The experimental results indicated that ECT in the recycle chamber could monitor the performance of the lower U-valve under different operating conditions for early detection of gas shortcut from the riser to the bubbling bed.Three main flow regimes in the riser and the differences between the reactors were identified by two sets of ECT sensors with pressure transducers.Finally,the effects of the operating conditions on the pressure drop in different parts of the DFBG was investigated. | Zhan Luo Yonglei Lin Qiuya Tu Wuqiang Yang Haigang Wang | 2020 | Particuology2020，18，4： | 4 |
| 17 | Optical vortex copier and regenerator in the Fourier domain显示文摘The generation and manipulation of optical vortices are of fundamental importance in a variety of promising applications. Here, we develop a nonlinear optical paradigm to implement self-and cross-convolution of optical vortex arrays, demonstrating the features of a vortex copier and regenerator. We use a phase-only spatial light modulator to prepare the 1064 nm invisible fundamental light to carry special optical vortex arrays and use a potassium titanyl phosphate crystal to perform type Ⅱ second-harmonic generation in the Fourier domain to achieve 532 nm visible structured vortices. Based on pure cross-convolution, we succeed in copying arbitrary-order single vortices as well as their superposition states onto a prearranged array of fundamental Gaussian spots. Also, based on the simultaneous effect of self-and cross-convolutions, we can expand the initial vortex lattices to regenerate more vortices carrying various higher topological charges. Our presented method of realizing an optical vortex copier and regenerator could find direct applications in optical manipulation, optical imaging, optical communication, and quantum information processing with structured vortex arrays. | XIAODONG QIU FANGSHU LI HAIGANG LIU XIANFENG CHEN LIXIANG CHEN | 2018 | Photonics Research2018，6，6： | 4 |
| 18 | α-Synuclein aggregation and transmission in Parkinson’s disease: a link to mitochondria and lysosome显示文摘The presence of intraneuronal Lewy bodies(LBs) and Lewy neurites(LNs) in the substantia nigra(SN) composed of aggregatedα-synuclein(α-syn) has been recognized as a hallmark of pathological changes in Parkinson’s disease(PD). Numerous studies have shown that aggregated α-syn is necessary for neurotoxicity. Meanwhile, the mitochondrial and lysosomal dysfunctions are associated with α-syn pathogenicity. The hypothesis that α-syn transmission in the human brain contributes to the instigation and progression of PD has provided insights into PD pathology. This review will provide a brief overview of increasing researches that shed light on the relationship of α-syn aggregation with mitochondrial and lysosomal dysfunctions, and highlight recent understanding of α-syn transmission in PD pathology. | Rui Wang Hongyang Sun Haigang Ren Guanghui Wang | 2020 | Science China(Life Sciences)2020，63，12： | 4 |
| 19 | Effect of Nitrogen Fertilizer on the Accumulation and Distribution of Dry Matter in Broomcorn Millet显示文摘[Objective] To understand the effect of nitrogen application on dry matter accumulation and allocation dynamics in broomcorn millet. [Method] The accumulation and distribution of dry matter were studied using cultivars Jin Shu 7 and Huang Mizi at different levels of nitrogen fertilizer at the jointing stage. [Result] The results showed that increasing N application led to the increase of green leaf area and the delay of leaf senescence, which was beneficial to the accumulation of dry matter.Appropriate nitrogen application(90 kg/hm2) could coordinate the translocation rate of dry matter among different plant parts, thereby enhancing the yield of broomcorn millet; among different organs, the contribution rate of stem to kernel was greater than that of leaf to kernel; there was obvious correlation between dry matter and yield. For Jin Shu 7, leaf area and dry weight of spike showed significant negative correlation with yield. [Conclusion] The formation of grain yield of broomcorn millet involved the accumulation and allocation of dry matter, the appropriate amount of nitrogen application(90 kg/hm2) could improve the rates of translocation and contribution of dry matter, thereby promoting the yield of broomcorn millet. | Ling CHEN Zhijun QIAO Junjie WANG Haigang WANG Xiaoning CAO Junli DONG | 2015 | Agricultural Science & Technology2015，16，7： | 4 |
| 20 | Poly-PR in C9ORF72-Related Amyotrophic Lateral Sclerosis/Frontotemporal Dementia Causes Neurotoxicity by Clathrin-Dependent Endocytosis显示文摘GGGGCC repeat expansions in the C9 ORF72 gene are the most common cause of amyotrophic lateral sclerosis and frontotemporal dementia(c9 ALS/FTD). It has been reported that hexanucleotide repeat expansions in C9 ORF72 produce five dipeptide repeat(DPR) proteins by an unconventional repeat-associated non-ATG(RAN)translation. Within the five DPR proteins, poly-PR and poly-GR that contain arginine are more toxic than the other DPRs(poly-GA, poly-GP, and poly-PA). Here, we demonstrated that poly-PR peptides transferred into cells by endocytosis in a clathrin-dependent manner, leading to endoplasmic reticulum stress and cell death. In SH-SY5 Y cells and primary cortical neurons, poly-PR activated JUN amino-terminal kinase(JNK) and increased the levels of p53 and Bax. The uptake of poly-PR peptides by cells was significantly inhibited by knockdown of clathrin or by chlorpromazine, an inhibitor that blocks clathrin-mediated endocytosis. Inhibition of clathrin-dependent endocytosis by chlorpromazine significantly blocked the transfer of poly-PR peptides into cells, and attenuated poly-PRinduced JNK activation and cell death. Our data revealed that the uptake of poly-PR undergoes clathrin-dependentendocytosis and blockade of this process prevents the toxic effects of synthetic poly-PR peptides. | Rui Wang Xingyun Xu Zongbing Hao Shun Zhang Dan Wu Hongyang Sun Chenchen Mu Haigang Ren Guanghui Wang | 2019 | Neuroscience Bulletin2019，35，5： | 4 |
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