|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Ultrafast photonic PCR显示文摘Nucleic acid amplification and quantification via polymerase chain reaction(PCR)is one of the most sensitive and powerful tools for clinical laboratories,precision medicine,personalized medicine,agricultural science,forensic science and environmental science.Ultrafast multiplex PCR,characterized by low power consumption,compact size and simple operation,is ideal for timely diagnosis at the point-of-care(POC).Although several fast/ultrafast PCR methods have been proposed,the use of a simple and robust PCR thermal cycler remains challenging for POC testing.Here,we present an ultrafast photonic PCR method using plasmonic photothermal light-to-heat conversion via photon–electron–phonon coupling.We demonstrate an efficient photonic heat converter using a thin gold(Au)film due to its plasmon-assisted high optical absorption(approximately 65%at 450 nm,the peak wavelength of heat source light-emitting diodes(LEDs)).The plasmon-excited Au film is capable of rapidly heating the surrounding solution to over 150℃ within 3 min.Using this method,ultrafast thermal cycling(30 cycles;heating and cooling rate of 12.7960.93℃ s^(-1) and 6.660.29℃ s^(-1),respectively)from 55℃(temperature of annealing)to 95℃(temperature of denaturation)is accomplished within 5 min.Using photonic PCR thermal cycles,we demonstrate here successful nucleic acid(λ-DNA)amplification.Our simple,robust and low cost approach to ultrafast PCR using an efficient photonic-based heating procedure could be generally integrated into a variety of devices or procedures,including on-chip thermal lysis and heating for isothermal amplifications. | Jun Ho Son Byungrae Cho SoonGweon Hong Sang Hun Lee Ori Hoxha Amanda J Haack Luke P Lee | 2015 | Light(Science & Applications)2015，4，1： | 2 |
| 2 | Triglyceride concentration and ischemic heart disease:an eight-year followup in the Copenhagen Male Study显示文摘 | Jeppesen J Hein HO Suadicani P | | Circulation0，，： | 2 |
| 3 | Regulation of growth hormone receptor/binding protein, insulin- like growth factor ternary complex system in human cirrhosis显示文摘 | Donaghy A J Delhanty P J Ho K K | 2002 | J Hepatol2002，36，6： | 1 |
| 4 | The relative contribution of Chkl and Chk2 to Adriamycin induced checkpoint 显示文摘 | HO C C SIU W Y CHOW J P | 2005 | Exp Cell Res2005，304，1： | 1 |
| 5 | Positional vertigo andnystagmus of central origin显示文摘 | Watson P Barber HO Deck J | 1981 | Can J Neurol Sci1981，8，： | 1 |
| 6 | Communication between eph- rinB2 and EphB4 within the osteoblast lineage 显示文摘 | Martin T J Allan E H Ho P W | 2010 | Adv Exp Med Biol2010，658，： | 1 |
| 7 | Value at Risk: applying the extreme value approach to Asian markets in the recent financial turmoil显示文摘 | Ho L C Burridge P Caddle J | 2000 | Pacific Basin Finance Journal2000，8，： | 1 |
| 8 | Hydrogen sulfide and its possible roles in myocardial ischemia in experimental rats 显示文摘 | Zhu Y Z Wang Z J Ho P | 2007 | J Appl Physiol2007，102，1： | 1 |
| 9 | Impact of Caregiving on Health and Quality of Life : A Comparative Popu- lation-based Study of Caregivers for Elderly Persons and Noncaregivers 显示文摘 | Ho S C Chan A Woo J Chong P and Sham A | 2009 | Journals of Gerontology Series A: Biologi- cal Sciences and Medical Sciences2009，64，： | 1 |
| 10 | Variants in linkage disequilibrium with the late cornified envelope gene cluster deletion are associated with susceptibility to psoriatic arthritis显示文摘 | Bowes J Flynn E Ho P | 2010 | Ann Rheum Dis2010，69，12： | 1 |
| 11 | Tissue factor expression correlates with tumor angiogenesis and invasiveness in human hepatocellular carcinoma 显示文摘 | Poon R T Lau C P Ho J W Yu W C Fan S T Wong J | 2003 | Clin Cancer Res2003，9，： | 1 |
| 12 | Structure and properties of cast binary Ti-Mo alloys 显示文摘 | HO W F JU C P CHERN-LIN J H | 1999 | Biomaterials1999，20，： | 1 |
| 13 | Medication Adherence: Its Importance in CardiovascuLar Outcomes显示文摘 | Ho P M Bryson C L Rumsfeld J S | 2009 | Circulation2009，119，23： | 1 |
| 14 | Zincblende and wurtzite phases in InN epilayers and their respective band transitions 显示文摘 | Specht P Ho J C Xu X | 2006 | J Cryst Growth2006，288，： | 1 |
| 15 | Structure and properties of cast binary Ti-Mo alloys显示文摘 | Ju C P Chern Lin J H | 1999 | Biomaterials1999，20，： | 1 |
| 16 | A New Shared Segment Protection Method for Survivable Networks with Guaranteed Recovery Time显示文摘 | Tapolcai J Ho P H Verchere D | 2008 | IEEE Transactions on Reliability2008，5，2： | 1 |
| 17 | Narcissism, extraversionand adolescents,self - presentation on Facebook 显示文摘 | Ong E Y L Ang R P Ho J C M | 2010 | Personality andIndividual Differences2010，50，2： | 1 |
| 18 | Electronic properties of armchair carbon nanotube array显示文摘 | WU S J HO Y H CHANG C P | 2006 | Physica E2006，32，12： | 1 |
| 19 | An anticipation model of potential customers' purchasing behavior based on clustering analysis and association rules analysis显示文摘 | Chang H J Huang L P Ho C L | 2007 | Expert System with Applications2007，32，3： | 1 |
| 20 | Adv Mater 显示文摘 | Gao J Li L Ho P L Mak G C Gu H Xu B | 2006 | 18(23): 3145-31482006，18，23： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员