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| 1 | OPS法玻璃化冷冻牛卵母细胞和囊胚显示文摘本文报道了利用拉细开口型细管 (Open Pulled Straw,OPS)方法进行玻璃化冷冻保存体外成熟(IVM)的牛卵母细胞及体外生产 (IVP)的牛囊胚的实验结果。实验 1的牛卵母细胞冷冻处理分 2组 ,组 1(G1 )是将卵母细胞在 IVM2 1 h时去除部分卵丘细胞后马上进行冷冻处理。组 2 (G2 )卵母细胞在 IVM6h时去除部分卵丘细胞 ,然后继续培养至 2 2 h冷冻 ;冷冻后的卵母细胞在液氮中保存 0 .5~ 2 h后解冻再继续 IVM1 h后与正常 IVM2 4h的对照组卵母细胞一起进行体外受精 (IVF)处理。实验 2则冷冻了来自IVP的 6、 7日龄的牛囊胚。结果显示 ,G1的卵母细胞 IVF后 8d的囊胚率 (2 2 .8% ,2 6/1 1 4)和孵化囊胚率 (1 4.9% ,1 7/1 1 4)都极显著地高于 G2 (分别为 2 .9% ,5 /1 71和 0 .5 % ,1 /1 71 ,P<0 .0 0 1 ) ;但两处理组的受精卵裂率差别不大 (分别为 48.2 %和 41 .5 % ,P>0 .3 )。冷冻第 6d和第 7d的体外囊胚 ,其冻后存活率和囊胚孵化率分别为 92 .7%、 89.2 %和 83 .6%、 66.7%。结果表明 ,利用 | 谭世俭 Henrik Callesen | 2002 | 广西农业生物科学2002，21，1： | 19 |
| 2 | 从基于服务的灵活性交易到跨行业能源系统的集成设计、规划和运行:丹麦的能源互联网理念(英文)显示文摘受到信息互联网的成功激励,世界范围内的能源行业都期望通过能源互联网技术(也被称为基于Web的智能电网或智能电网2.0),为全行业提供基于先进互联及分布式能源智能管理的全新应用。作为欧洲智能电网领域的领先国家,丹麦对于能源互联网的发展有着较为独特的视角。文中在调研了丹麦大量研究与研发活动的基础上,重点介绍丹麦所秉持的两类重要的能源互联网技术理念,即基于服务的灵活性交易,以及跨行业能源系统的集成设计、规划和运行。丹麦希望基于上述理念,为能源工业的不同利益相关方带来全新的商业机会和挑战,并进一步使用新型的技术解决方案促进能源市场的转型和能源互联网的发展。 | 尤石 林今 胡俊杰 宗毅 Henrik W.BINDNER | 2015 | 中国电机工程学报2015，35，14： | 19 |
| 3 | 德国低能耗建筑技术体系及发展趋势显示文摘总结归纳了德国低能耗建筑的分类标准,分析了三种不同标准低能耗建筑相应的设计方法与技术系统;介绍了低能耗建筑技术的发展新动向和德国低能耗建筑推广策略与既有建筑节能改造的案例。 | 卢求 Henrik Wings | 2007 | 建筑学报2007，，9： | 18 |
| 4 | 与新生儿缺氧缺血性脑病相关的热点问题显示文摘 | 朱长连 Stephen A.Back Steven W.Levison Sandra Juul Richard A.Polin Henrik Hagberg George Simbruner 杜立中 | 2009 | 中国循证儿科杂志2009，4，5： | 17 |
| 5 | Low-FODMAP diet reduces irritable bowel symptoms in patients with inflammatory bowel disease显示文摘AIM To investigate the effect of a low-FODMAP diet on irritable bowel syndrome(IBS)-like symptoms in patients with inflammatory bowel disease(IBD).METHODS This was a randomised controlled open-label trial of patients with IBD in remission or with mild-to-moderate disease and coexisting IBS-like symptoms(Rome III) randomly assigned to a Low-FODMAP diet(LFD) or a normal diet(ND) for 6 wk between June 2012 andDecember 2013. Patients completed the IBS symptom severity system(IBS-SSS) and short IBD quality of life questionnaire(SIBDQ) at weeks 0 and 6. The primary end-point was response rates(at least 50-point reduction) in IBS-SSS at week 6 between groups; secondary end-point was the impact on quality of life. RESULTS Eighty-nine patients, 67(75%) women, median age 40, range 20-70 years were randomised: 44 to LFD group and 45 to ND, from which 78 patients completed the study period and were included in the final analysis(37 LFD and 41 ND). There was a significantly larger proportion of responders in the LFD group(n = 30, 81%) than in the ND group(n = 19, 46%);(OR = 5.30; 95%CI: 1.81-15.55, P < 0.01). At week 6, the LFD group showed a significantly lower median IBSSSS(median 115; inter-quartile range [IQR] 33-169) than ND group(median 170, IQR 91-288), P = 0.02. Furthermore, the LFD group had a significantly greater increase in SIBDQ(median 60, IQR 51-65) than the ND group(median 50, IQR 39-60), P < 0.01.CONCLUSION In a prospective study, a low-FODMAP diet reduced IBS-like symptoms and increased quality of life in patients with IBD in remission. | Natalia Pedersen Dorit Vedel Ankersen Maria Felding Henrik Wachmann Zsuzsanna Végh Line Molzen Johan Burisch Jens Rikardt Andersen Pia Munkholm | 2017 | World Journal of Gastroenterology2017，23，18： | 15 |
| 6 | Feasibility of cold snare polypectomy in Japan: A pilot study显示文摘AIM: To investigate the feasibility of cold snare polypectomy(CSP) in Japan.METHODS: The outcomes of 234 non-pedunculated polyps smaller than 10 mm in 61 patients who underwent CSP in a Japanese referral center were retrospectively analyzed. The cold snare polypectomies were performed by nine endoscopists with no prior experience in CSP using an electrosurgical snare without electrocautery.RESULTS: CSPs were completed for 232 of the 234 polyps. Two(0.9%) polyps could not be removed without electrocautery. Immediate postpolypectomy bleeding requiring endoscopic hemostasis occurred in eight lesions(3.4%; 95%CI: 1.1%-5.8%), but all were easily managed. The incidence of immediate bleeding after CSP for small polyps(6-9 mm) was significantly higher than that of diminutive polyps(≤ 5 mm; 15% vs 1%, respectively). Three(5%) patients complained of minor bleeding after the procedure but required no intervention. The incidence of delayed bleeding requiringendoscopic intervention was 0.0%(95%CI: 0.0%-1.7%). In total, 12% of the resected lesions could not be retrieved for pathological examination. Tumor involvement in the lateral margin could not be histologically assessed in 70(40%) lesions.CONCLUSION: CSP is feasible in Japan. However, immediate bleeding, retrieval failure and uncertain assessment of the lateral tumor margin should not be underestimated. Careful endoscopic diagnosis before and evaluation of the tumor residue after CSP are recommended when implementing CSP in Japan. | Yoji Takeuchi Takeshi Yamashina Noriko Matsuura Takashi Ito Mototsugu Fujii Kengo Nagai Fumi Matsui Tomofumi Akasaka Noboru Hanaoka Koji Higashino Hiroyasu Iishi Ryu Ishihara Henrik Thorlacius Noriya Uedo | 2015 | World Journal of Gastrointestinal Endoscopy2015，7，17： | 14 |
| 7 | The coupling of dynamics and permeability in the hydrocarbon accumulation period controls the oil-bearing potential of low permeability reservoirs:a case study of the low permeability turbidite reservoirs in the middle part of the third member of Shahejie显示文摘The relationships between permeability and dynamics in hydrocarbon accumulation determine oilbearing potential(the potential oil charge) of low permeability reservoirs.The evolution of porosity and permeability of low permeability turbidite reservoirs of the middle part of the third member of the Shahejie Formation in the Dongying Sag has been investigated by detailed core descriptions,thin section analyses,fluid inclusion analyses,carbon and oxygen isotope analyses,mercury injection,porosity and permeability testing,and basin modeling.The cutoff values for the permeability of the reservoirs in the accumulation period were calculated after detailing the accumulation dynamics and reservoir pore structures,then the distribution pattern of the oil-bearing potential of reservoirs controlled by the matching relationship between dynamics and permeability during the accumulation period were summarized.On the basis of the observed diagenetic features and with regard to the paragenetic sequences,the reservoirs can be subdivided into four types of diagenetic fades.The reservoirs experienced two periods of hydrocarbon accumulation.In the early accumulation period,the reservoirs except for diagenetic fades A had middle to high permeability ranging from 10 × 10^(-3) μm^2 to4207 × 10^(-3) μm^2.In the later accumulation period,the reservoirs except for diagenetic fades C had low permeability ranging from 0.015 × 10^(-3)~ μm^2 to 62 × 10^(-3) μm^2.In the early accumulation period,the fluid pressure increased by the hydrocarbon generation was 1.4-11.3 MPa with an average value of 5.1 MPa.and a surplus pressure of1.8-12.6 MPa with an average value of 6.3 MPa.In the later accumulation period,the fluid pressure increased by the hydrocarbon generation process was 0.7-12.7 MPa with an average value of 5.36 MPa and a surplus pressure of1.3-16.2 MPa with an average value of 6.5 MPa.Even though different types of reservoirs exist,all can form hydrocarbon accumulations in the early accumulation period.Such types of reservoirs can form hydrocarbon accumulation with high accumulation dynamics:however,reservoirs with diagenetic facies A and diagenetic fades B do not develop accumulation conditions with low accumulation dynamics in the late accumulation period for very low permeability.At more than 3000 m burial depth,a larger proportion of turbidite reservoirs are oil charged due to the proximity to the source rock.Also at these depths,lenticular sand bodies can accumulate hydrocarbons.At shallower depths,only the reservoirs with oil-source fault development can accumulate hydrocarbons.For flat surfaces,hydrocarbons have always been accumulated in the reservoirs around the oil-source faults and areas near the center of subsags with high accumulation dynamics. | Tian Yang Ying-Chang Cao Yan-Zhong Wang Henrik Friis Beyene Girma Haile Ke-Lai Xi Hui-Na Zhang | 2016 | Petroleum Science2016，13，2： | 10 |
| 8 | Nasal continuous positive airway pressure combined with surfactant and NO for treatment of respiratory distress syndrome, prevention of bronchopulmonary dysplasia, and brain protection显示文摘 | Henrik Verder | 2010 | Chinese Medical Journal2010，，20： | 8 |
| 9 | INT-767 improves histopathological features in a dietinduced ob/ob mouse model of biopsy-confirmed nonalcoholic steatohepatitis显示文摘AIM To characterize the efficacy of the dual FXR/TGR5 receptor agonist INT-767 upon histological endpoints in a rodent model of diet-induced and biopsy-confirmed non-alcoholic steatohepatitis(NASH).METHODS The effects of INT-767 on histological features of NASH were assessed in two studies using Lep^(ob/ob)(ob/ob) NASH mice fed the AMLN diet(high fat with transfat, cholesterol and fructose). In a proof-of-conceptstudy, Lep^(ob/ob)(ob/ob) NASH mice were first dosed with INT-767(3 or 10 mg/kg for 8 wk). A second ob/ob NASH study compared INT-767(3 and 10 mg/kg) to obeticholic acid(OCA)(10 or 30 mg/kg; 16 wk). Primary histological endpoints included qualitative and quantitative assessments of NASH. Other metabolic and plasma endpoints were also assessed. A comparative assessment of INT-767 and OCA effects on drug distribution and hepatic gene expression was performed in C57 Bl/6 mice on standard chow. C57 Bl/6 mice were orally dosed with INT-767 or OCA(1-30 mg/kg) for 2 wk, and expression levels of candidate genes were assessed by RNA sequencing and tissue drug levels were measured by liquid chromatography tandem-mass spectrometry.RESULTS INT-767 dose-dependently(3 and 10 mg/kg, PO, QD, 8 wk) improved qualitative morphometric scores on steatohepatitis severity, inflammatory infiltrates and fibrosis stage. Quantitative morphometric analyses revealed that INT-767 reduced parenchymal collagen area, collagen fiber density, inflammation(assessed by Galectin-3 immunohistochemistry) and hepatocyte lipid droplet area following INT-767 treatment. In a comparative study(16 wk), the FXR agonists OCA(10 and 30 mg/kg) and INT-767(3 and 10 mg/kg) both improved NASH histopathology, with INT-767 exerting greater therapeutic potency and efficacy than OCA. Mechanistic studies suggest that both drugs accumulate similarly within the liver and ileum, however, the effects of INT-767 may be driven by enhanced hepatic, but not ileal, FXR function. CONCLUSION These findings confirm the potential utility of FXR and dual FXR/TGR5 activation as disease intervention strategies in NASH. | Jonathan D Roth Michael Feigh Sanne S Veidal Louise KD Fensholdt Kristoffer T Rigbolt Henrik H Hansen Li C Chen Mathieu Petitjean Weslyn Friley Niels Vrang Jacob Jelsing Mark Young | 2018 | World Journal of Gastroenterology2018，24，2： | 7 |
| 10 | Next-Generation Stool DNA Test Accurately Detects Colorectal Cancer and Large Adenomas显示文摘 | David A. Ahlquist Hongzhi Zou Michael Domanico Douglas W. Mahoney Tracy C. Yab William R. Taylor Malinda L. Butz Stephen N. Thibodeau Linda Rabeneck Lawrence F. Paszat Kenneth W. Kinzler Bert Vogelstein Niels Chr. Bjerregaard S?ren Laurberg Henrik Toft S? | 2012 | Gastroenterology2012，，2： | 7 |
| 11 | Helicobacter pylori infection and low serum pepsinogen I level as risk factors for gastric carcinoma显示文摘AIM: To study whether examination of CagA antibodies could increase the odds ratio for gastric cancer in a casecontrol study, and how often other serum markers of gastric cancer risk could be found in Helicobacter pylorinegative patients.METHODS: H pylori CagA and parietal cell antibodies(PCAs), and serum pepsinogen Ⅰ (SPGI) levels were compared between patients with gastric cancer and controls who received endoscopic examination due to reasons other than gastrointestinal malignancy.RESULTS: The odds ratio (OR) for gastric cancer was2.9 (95% CI 1.4-5.8) in H pylori+ patients, and 2.4 (95%CI 1.2-4.9) in CagA+ patients. When results of H pylori and CagA antibodies were combined, OR increased to5.0 (95% CI 2.5-10.0). Furthermore, if cardia cancer patients were excluded, the OR increased to 6.8 (95% CI3.1-14.8). Among patients with a low SPGI level, the OR was 12.0 (95% CI 4.1-35.3). However, the risk was significant only in the older age group. The number of patients with low SPGI was significantly higher in H pylori-/CagA+ patients as compared to other cancer patients.CONCLUSION: Examination of both H pylori and CagA antibodies increases the OR for gastric cancer in our casecontrol study. CagA antibodies are important in detecting previous H pylori infection in advanced atrophic gastritis or cancer when spontaneous decline of H pylori antibodies occurs. SPGI may be helpful in screening elderly gastric cancer patients. | Arto Kokkola Johanna Louhimo Pauli Puolakkainen Henrik Alfthan Caj Haglund Hilpi Rautelin | 2005 | World Journal of Gastroenterology2005，11，7： | 6 |
| 12 | 功能性电刺激在步态功能恢复中的技术进展(英文)显示文摘回顾了近几十年功能性电刺激在步态恢复中的技术进展。功能性电刺激干预的目的是对运动障碍(例如中风、脊髓损伤、多发性硬化症及其他)的人群进行功能性地步态恢复与康复治疗。近几年由于微纳米电子科技的快速发展,此项技术已被广泛应用到实际当中。综述涵盖了在学术界研究开发和目前市场上可用的神经假肢,并对这些系统进行了深入地分析与讨论。尤其强调了传感技术和控制策略在系统中的应用,展望了该研究领域的发展前景,并提出结合功能性电刺激与机械外骨骼作为一项新兴方法来解决目前基于功能性电刺激的神经假肢研究中的困难问题。 | 孟琳 Bernd Porr Henrik Gollee | 2017 | 仪器仪表学报2017，38，6： | 6 |
| 13 | Arabidopsis Mutants and the Network of M icrotu bu le-Associated Functions显示文摘在早优核质,微导管系统从事有丝分裂,细胞内部的运输,和基于鞭子的活动性。在植物系,多细胞的身体的进化包含了一些核心函数,其它的损失,和与多细胞的植物习惯联系的新微导管函数的详尽阐述的保存。这多样化被保存的两个(animal/fungi-like ) 和小说的存在反映(似植物) 把微导管相关的功能编码为 Arabidopsis 染色体的序列。微导管异种的收集在最近的年很快成长了。这些异种介绍大量显型,与微导管系统的功能的多样化的假设一致。在这评论,我们集中于变异的分析并且特别地,为指出基因功能的小径作为一个珍贵工具讨论两倍变异的分析。未来挑战将是在植物定义微导管功能的基因、物理的相互作用的完全的网络。除了在 MAPome 的功能的分析考察最近的进步,我们在场在微导管损害的 Arabidopsis 异种的一个联机数据库工作。 | Henrik Buschmann Clive W. Lloyd | 2008 | Molecular Plant2008，1，6： | 6 |
| 14 | Effects of a novel bacterial phytase expressed in Aspergillus Oryzae on digestibility of calcium and phosphorus in diets fed to weanling or growing pigs显示文摘In 2 experiments, 48 weanling (initial BW: 13.5 ± 2.4 kg, Exp. 1) and 24 growing pigs (initial BW: 36.2 ± 4.0 kg, Exp. 2) were used to determine effects of a novel bacterial 6-phytase expressed in Aspergillus oryzae on the apparent total tract digestibility (ATTD) of phosphorus and calcium in corn-soybean meal diets fed to weanling and growing pigs. In Exp. 1 and 2, pigs were randomly allotted to 6 dietary treatments using a randomized complete block design and a balanced 2 period changeover design, respectively. In both experiments, 6 diets were formulated. The positive control diet was a corn-soybean meal diet with added inorganic phosphorus (Exp. 1: 0.42 and 0.86% standardized total tract digestible phosphorus and total calcium, respectively; Exp. 2: 0.32 and 0.79% standardized total tract digestible phosphorus and total calcium, respectively). A negative control diet and 4 diets with the novel phytase (Ronozyme HiPhos, DSM Nutritional Products Inc., Parsippany, NJ) added to the negative control diet at levels of 500, 1,000, 2,000, and 4,000 phytase units (FYT)/kg were also formulated. In Exp. 1, the ATTD of phosphorus was greater (P < 0.01) for the positive control diet (60.5%) than for the negative control diet (40.5%), but increased (linear and quadratic, P < 0.01) as phytase was added to the negative control diet (40.5% vs. 61.6%, 65.1%, 68.7%, and 68.0%). The breakpoint for the ATTD of phosphorus (68.4%) was reached at a phytase inclusion level of 1,016 FYT/kg. In Exp. 2, the ATTD of phosphorus was greater (P < 0.01) for the positive control diet (59.4%) than for the negative control diet (39.8%) and increased (linear and quadratic, P < 0.01) as phytase was added to the negative control diet (39.8% vs. 58.1%, 65.4%, 69.1%, and 72.8%). The breakpoint for the ATTD of phosphorus (69.1%) was reached at a phytase inclusion level of 801 FYT/kg. In conclusion, the novel bacterial 6-phytase improved the ATTD of phosphorus and calcium in both weanling and growing pigs. The optimum level of inclusion for this phytase is 800 to 1,000 FYT/kg of complete feed to maximize ATTD of phosphorus and calcium in weanling and growing pigs. | Ferdinando Nielsen Almeida Rommel Casilda Sulabo Hans Henrik Stein | 2013 | Journal of Animal Science and Biotechnology2013，4，2： | 6 |
| 15 | Metallic mineral deposits in the Nordic countries显示文摘 | Paer Weihed Pasi Eilu Rune B. Larsen Henrik Stendal Mikko Tontti | 2008 | Episodes2008，31，1： | 6 |
| 16 | Dual Targeting to Mitochondria and Chloroplasts: Characterization of Thr-tRNA Synthetase Targeting Peptide显示文摘 | Anna-Karin Berglund Erika Spanning Henrik Biverstahl Gianluca Maddalo Christian Tellgren-Roth Lena Maler Elzbieta Glaser | 2009 | Molecular Plant2009，2，6： | 6 |
| 17 | 围生期宫内感染与早产儿脑损伤的相关问题显示文摘 | Henrik Hagberg Carina Mallard 俞惠民 王来栓 | 2009 | 中国循证儿科杂志2009，4，4： | 6 |
| 18 | Metabolic and hepatic effects of liraglutide,obeticholic acid and elafibranor in diet-induced obese mouse models of biopsy-confirmed nonalcoholic steatohepatitis显示文摘AIM To evaluate the pharmacodynamics of compounds in clinical development for nonalcoholic steatohepatitis(NASH) in obese mouse models of biopsy-confirmedNASH.METHODS Male wild-type C57 BL/6 J mice(DIO-NASH) and Lep^(ob/ob)(ob/ob-NASH) mice were fed a diet high in trans-fat(40%), fructose(20%) and cholesterol(2%) for 30 and 21 wk, respectively. Prior to treatment, all mice underwent liver biopsy for confirmation and stratification of liver steatosis and fibrosis, using the nonalcoholic fatty liver disease activity score(NAS) and fibrosis staging system. The mice were kept on the diet and received vehicle, liraglutide(0.2 mg/kg, SC, BID), obeticholic acid(OCA, 30 mg/kg PO, QD), or elafibranor(30 mg/kg PO, QD) for eight weeks. Within-subject comparisons were performed on changes in steatosis, inflammation, ballooning degeneration, and fibrosis scores. In addition, compound effects were evaluated by quantitative liver histology, including percent fractional area of liver fat, galectin-3, and collagen 1 a1.RESULTS Liraglutide and elafibranor, but not OCA, reduced body weight in both models. Liraglutide improved steatosis scores in DIO-NASH mice only. Elafibranor and OCA reduced histopathological scores of hepatic steatosis and inflammation in both models, but only elafibranor reduced fibrosis severity. Liraglutide and OCA reduced total liver fat, collagen 1 a1, and galectin-3 content, driven by significant reductions in liver weight. The individual drug effects on NASH histological endpoints were supported by global gene expression(RNA sequencing) and liver lipid biochemistry.CONCLUSION DIO-NASH and ob/ob-NASH mouse models show distinct treatment effects of liraglutide, OCA, and elafibranor, being in general agreement with corresponding findings in clinical trials for NASH. The present data therefore further supports the clinical translatability and utility of DIO-NASH and ob/ob-NASH mouse models of NASH for probing the therapeutic efficacy of compounds in preclinical drug development for NASH. | Kirstine S Tolbol Maria NB Kristiansen Henrik H Hansen Sanne S Veidal Kristoffer TG Rigbolt Matthew P Gillum Jacob Jelsing Niels Vrang Michael Feigh | 2018 | World Journal of Gastroenterology2018，24，2： | 5 |
| 19 | Voltage rise mitigation for solar PV integration at LV grids Studies from PVNET.dk显示文摘Solar energy from photovoltaic(PV)is among the fastest developing renewable energy systems worldwide.Driven by governmental subsidies and technological development,Europe has seen a fast expansion of solar PV in the last few years.Among the installed PV plants,most of them are situated at the distribution systems and bring various operational challenges such as power quality and power flow management.The paper discusses the modelling requirements for PV system integration studies,as well as the possible techniques for voltage rise mitigation at low voltage(LV)grids for increasing PV penetration.Potential solutions are listed and preliminary results are presented. | Guangya YANG Francesco MARRA Miguel JUAMPEREZ Søren Bækhøj KJÆR Seyedmostafa HASHEMI JacobØSTERGAARD Hans Henrik IPSEN Kenn H.B.FREDERIKSEN | 2015 | Journal of Modern Power Systems and Clean Energy2015，3，3： | 5 |
| 20 | CYP24A1 inhibition facilitates the anti-tumor effect of vitamin D3 on colorectal cancer cells显示文摘AIM:The effects of vitamin D3 have been investigated on various tumors, including colorectal cancer (CRC). 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1), the enzyme that inactivates the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25-D3), is considered to be the main enzyme determining the biological halflife of 1,25-D3. During colorectal carcinogenesis, the expression and concentration of CYP24A1 increases significantly, suggesting that this phenomenon could be responsible for the proposed efficacy of 1,25-D3 in the treatment of CRC. The aim of this study was to investigate the anti-tumor effects of vitamin D3 on the human CRC cell line Caco-2 after inhibition of the cytochrome P450 component of CYP24A1 activity. METHODS:We examined the expression of CYP24A1 mRNA and the effects of 1,25-D3 on the cell line Caco-2 after inhibition of CYP24A1. Cell viability and proliferation were determined by means of sulforhodamine-B staining and bromodeoxyuridine incorporation, respectively, while cytotoxicity was estimated via the lactate dehydrogenase content of the cell culture supernatant. CYP24A1 expression was measured by realtime reverse transcription polymerase chain reaction. A number of tetralone compounds were synthesized to investigate their CP24A1 inhibitory activity. RESULTS:In response to 1,25-D3, CYP24A1 mRNA expression was enhanced significantly, in a time- and dose-dependent manner. Caco-2 cell viability and proliferation were not influenced by the administration of 1,25-D3 alone, but were markedly reduced by coadministration of 1,25-D3 and KD-35, a CYP24A1-inhibiting tetralone. Our data suggest that the mechanism of action of co-administered KD-35 and 1,25-D3 does not involve a direct cytotoxic effect, but rather the inhibition of cell proliferation. CONCLUSION:These findings demonstrate that the selective inhibition of CYP24A1 by compounds such as KD-35 may be a new approach for enhancement of the anti-tumor effect of 1,25-D3 on CRC. | János P Kósa Péter Horváth János Wlfling Dóra Kovács Bernadett Balla Péter Mátyus Evelin Horváth Gábor Speer István Takács Zsolt Nagy Henrik Horváth Péter Lakatos | 2013 | World Journal of Gastroenterology2013，19，17： | 5 |
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