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| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | 16年随访的前瞻性队列中521120名个体的膳食脂肪摄入与总死亡率和特定原因死亡率的关系显示文摘饱和脂肪摄入与心血管健康相关性的证据存在争议。不饱和脂肪和总死亡率及心血管病死亡率的相关性存在不一致性,并且关于非心血管病死亡率的数据较少。该文旨在评估膳食脂肪与总死亡率和特定原因死亡率的关系。方法和结果:研究者分析了来自随访16年的美国国立卫生研究院一美国退休人员协会膳食与健康研究年龄50~71岁的参与者521 120人的数据。通过食品频率问卷评估饱和脂肪酸(saturatedfatty acid,SFA )、反式脂肪酸(trans-fatty acid,TFA)、单不饱和脂肪酸(monounsaturated fatty acid,MUFA)与多不饱和脂肪酸(polyunsaturated fattyacid,PUFA)的摄入量。 | 陈嘉睿(译) 练桂丽(审校) Zhuang P Zhang Y He W Chen X Chen J He L Mao L Wu F Jiao J | 2019 | 中华高血压杂志2019，27，4： | 7 |
| 3 | The role of the N-methyl-D-aspartate receptor in the proliferation of adult hippocampal neural stem and precursor cells显示文摘New neurons are continuously generated from resident pools of neural stem and precursor cells(NSPCs)in the adult brain.There are multiple pathways through which adult neurogenesis is regulated,and here we review the role of the N-methyl-D-aspartate receptor(NMDAR)in regulating the proliferation of NSPCs in the adult hippocampus.Hippocampal-dependent learning tasks,enriched environments,running,and activity-dependent synaptic plasticity,all potently up-regulate hippocampal NSPC proliferation.We first consider the requirement of the NMDAR in activity-dependent synaptic plasticity,and the role the induction of synaptic plasticity has in regulating NSPCs and newborn neurons.We address how specific NMDAR agonists and antagonists modulate proliferation,both in vivo and in vitro,and then review the evidence supporting the hypothesis that NMDARs are present on NSPCs.We believe it is important to understand the mechanisms underlying the activation of adult neurogenesis,given the potential that endogenous stem cell populations have for repopulating the hippocampus with functional new neurons.In conditions such as age-related memory decline,neurodegeneration and psychiatric disease,mature neurons are lost or become defective;as such,stimulating adult neurogenesis may provide a therapeutic strategy to overcome these conditions. | TAYLOR Chanel J HE RongQiao BARTLETT Perry F | 2014 | Science China(Life Sciences)2014，57，4： | 6 |
| 4 | Expression and release of IL-29 by mast cells and modulation of mast cell behavior by IL-29显示文摘 | He, S Zhang, H Chen, H Yang, H Huang, T Chen, Y Lin, J Wang, F Chen, X Li, TL Yang, P | 2010 | 南京医科大学学报(自然科学版)2010，30，11： | 6 |
| 5 | Induction of mucosal and systemic immune response by single-dose oral immunization with biodegradable microparticles containing DNA encoding HBsAg显示文摘 | He XW Wang F Jiang L Li J Liu SK Xiao ZY Jin XQ Zhang YN He Y Li K Guo YJ Sun SH | 2005 | 第二军医大学学报2005，26，10： | 5 |
| 6 | Disruption of sphingolipid homeostasis by myrioein, a myeotoxin, reduces thymie and splenic T-lymphocyte populations显示文摘 | Johnson V J He Q Osuchowski M F | 2004 | Toxicology2004，201，13： | 1 |
| 7 | prognostic value of serum CA19 - 9 levels in pancreatic adenocarcinoma 显示文摘 | Tian FZ Appert HE Mules F J | | Ann Surg0，215，4： | 1 |
| 8 | PZT-based materials with bilayered structure preparation and ferroelectric properties显示文摘 | He Z M Ma J Zhang R F | 2003 | Journal of the European Creamic Society2003，23，11： | 1 |
| 9 | Respiratory evolution of patient with mucoviscidosis treated with mucolytic agents plus domasealfa显示文摘 | Derelle J Bertolo HE Marchal F | 1998 | Arch Pediatr1998，5，4： | 1 |
| 10 | Piecewise-Koszul algebras 显示文摘 | Lu J F He J W Lu D M | 2007 | Scinece in China Series A: Mathematics2007，50，： | 1 |
| 11 | Dissolved organic matter (DOM) in recycled leachate of bioreactor landfill 显示文摘 | He P J Xue J F Shao L M | 2006 | Water Res2006，40，7： | 1 |
| 12 | Inverse application of charge simulation method in detecting faulty ceramic insulators and processing influence from tower 显示文摘 | HE W YANG F WANG J G | 2006 | IEEE Transactions on Magnetics2006，42，4： | 1 |
| 13 | Newly synthesized dyes and their polymer/Glass composites for one-and two-photon pumped solid-state cavity lasing显示文摘 | Zhao C F He G S Bhawalkar J D | 1995 | Chem Mater1995，，7： | 1 |
| 14 | Sea surface temperature variability in the north side of the South China Sea over the last 55 years revealed by coral δ^18O records显示文摘 | Peng Z C He J F Chen T G | 2003 | Geochimica et Cosmochimica Acta2003，67，18： | 1 |
| 15 | Respiratory evolution of patient with mucoviscidosis treated with mucolytic agents plus domase alfa显示文摘 | Derelle J Bertolo HE Marchal F | 1998 | Arch-pediatr1998，5，4： | 1 |
| 16 | Methotrexate-Based bilateral uterine arterial chemoem- bolizatioia for treatment of caesarean scar pregnancy 显示文摘 | Liang F He J | 2010 | Acta Obstet Gynecol Scand2010，89，： | 1 |
| 17 | China's regional difference of water resource use efficiency and influencing factors显示文摘 | Qian W J He C F | 2011 | China Population Resources and Environment2011，21，2： | 1 |
| 18 | Narcissus analysis for cooled staring IR system 显示文摘 | He F Y Cui J C Feng S L | 2007 | SPIE2007，6772，67： | 1 |
| 19 | Hemoglobin and iron handling in brain after subarachnoid hemorrhage and the effect of deferoxa- mine on early brain injury显示文摘 | LEE J Y KEEP R F HE Y | 2010 | J Cereb Blood Flow Metab2010，30，11： | 1 |
| 20 | Adaptive fast consensus algorithm for distributed sensor fusion显示文摘 | Xi F He J Liu Z | 2010 | Signal Processing2010，90，5： | 1 |
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