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| 1 | Correlation of the temporal and spatial expression patterns of HQT with the biosynthesis and accumulation of chlorogenic acid in Lonicera japonica flowers显示文摘Hydroxycinnamoyl-CoA quinate transferase(HQT)is one of the key enzymes in the biosynthesis of chlorogenic acid(CGA)in the flowers of Lonicera japonica.However,the spatiotemporal expression patterns of HQT and its relationship to the dynamics of CGA biosynthesis,transport,and storage remain largely unknown.In this study,we collected L.japonica flower samples at different growth stages(S1–S6)and examined the spatiotemporal expression pattern of HQT and the dynamic accumulation patterns of CGA using a combination of molecular and cytological techniques.Our results suggest that the spatiotemporal expression pattern of HQT is directly correlated with dynamic changes in CGA accumulation and distribution in L.japonica flowers.We further show that CGA is synthesized primarily in the cytoplasm and chloroplasts.CGA synthesized in the cytoplasm first accumulates in specialized vesicles and is then transferred to large central vacuoles for storage by fusion of CGA-containing vesicles with vacuoles.Furthermore,CGA synthesized in the chloroplasts appears to be transferred into the vacuoles for storage by direct membrane fusion between the tonoplast and the disrupted chloroplast membranes.Collectively,our results suggest that CGA is synthesized in chloroplasts and cytoplasm and finally transferred to the vacuole for long-term storage. | Yanqun Li Dexin Kong Mei Bai Hanjun He Haiyang Wang Hong Wu | 2019 | Horticulture Research2019，6，1： | 7 |
| 2 | Application and optimization of solid-state fermentation process for enhancing polygalacturonase production by Penicillium expansum显示文摘Penicillium expansum 3.5425 was applied in solid-state fermentation(SSF)of agricultural wastes for polygalacturonase biosynthesis.Among various carbon additives,apple pomace was most suitable for the biosynthesis of polygalacturonase(1440.57 U/g).Optimization of medium parameters using rotational orthogonal design(ROD)experiment combined with optimal fermentation conditions resulted in a 2.72-fold increase in the polygalacturonase production.By using ammonium sulphate precipitation,ion-exchange and gel-permeation chromatography,the polygalacturonase produced by P.expansum 3.5425 was finally purified which had specific activity of 19269 U/mg and molecular weight of 30 kDa.The enzyme was remarkably active in the pH range of 3-5 and at 50℃,which makes it more acceptable in the industrial application.Besides,partially purified polygalacturonase(875.15 U/mL)was used for apple juice clarification and the clarity at 0.4 mL/kg was maximum,which reveals a great potential of polygalacturonase in food industry. | Mingming Zhu Hongju He Mingtao Fan Hanjun Ma Haiwei Ren Jie Zeng Haiyan Gao | 2018 | International Journal of Agricultural and Biological Engineering2018，11，6： | 2 |
| 3 | LW-NIR hyperspectral imaging for rapid prediction of TVC in chicken flesh显示文摘Total viable count(TVC)is often used as an important indicator for chicken freshness evaluation.In this study,112 fresh chicken flesh samples were acquired after slaughtered and their hyperspectral images were collected in the LW-NIR(900-1700 nm)range.The full LW-NIR spectra(486 wavebands)within the images were extracted and applied to related to reference TVC values measured in different storage periods,using partial least squares regression(PLSR)algorithm,resulting in high correlation coefficients(R)and low root mean square errors(RMSE),for either raw spectra or pretreatment spectra.By using regression coefficients(RC)method,20,18,17 and 20 optimal wavebands were respectively selected from raw spectra,baseline correction(BC)spectra,Savitzky-Golay convolution smoothing(SGCS)spectra and standard normal variate(SNV)spectra and applied for the optimization of original full waveband PLSR model.By comparison,RC-PLSR model based on the SGCS spectra showed a better performance in TVC prediction with RC of 0.98 and RMSEC of 0.35 log10 CFU/g in calibration set,and RP of 0.98 and RMSEP of 0.44 log10 CFU/g in prediction set.At last,by transferring the best RC-PLSR model,the dynamic TVC change during the storage was visualized by color maps to indicate the TVC spoilage degree.The overall study revealed that LW-NIR hyperspectral imaging combined with PLSR could be used to predict the freshness of chicken flesh. | Hui Wang Hongju He Hanjun Ma Fusheng Chen Zhuangli Kang Mingming Zhu Zhengrong Wang Shengming Zhao Rongguang Zhu | 2019 | International Journal of Agricultural and Biological Engineering2019，12，3： | 2 |
| 4 | Quick assessment of chicken spoilage based on hyperspectral NIR spectra combined with partial least squares regression显示文摘Pseudomonas spp.and Enterobacteriaceae are dominant spoilage bacteria in chicken during cold storage(0°C-4°C).In this study,high resolution spectra in the range of 900-1700 nm were acquired and preprocessed using Savitzky-Golay convolution smoothing(SGCS),standard normal variate(SNV)and multiplicative scatter correction(MSC),respectively,and then mined using partial least squares(PLS)algorithm to relate to the total counts of Pseudomonas spp.and Enterobacteriaceae(PEC)of fresh chicken breasts to predict PEC rapidly.The results showed that with full 900-1700 nm range wavelength,MSC-PLS model built with MSC spectra performed better than PLS models with other spectra(RAW-PLS,SGCS-PLS,SNV-PLS),with correlation coefficient(RP)of 0.954,root mean square error of prediction(RMSEP)of 0.396 log10 CFU/g and residual predictive deviation(RPD)of 3.33 in prediction set.Based on the 12 optimal wavelengths(902.2 nm,905.5 nm,923.6 nm,938.4 nm,946.7 nm,1025.7 nm,1124.4 nm,1211.6 nm,1269.2 nm,1653.7 nm,1691.8 nm and 1693.4 nm)selected from MSC spectra by successive projections algorithm(SPA),SPA-MSC-PLS model had RP of 0.954,RMSEP of 0.397 log10 CFU/g and RPD of 3.32,similar to MSC-PLS model.The overall study indicated that NIR spectra combined with PLS algorithm could be used to detect the PEC of chicken flesh in a rapid and non-destructive way. | Shengqi Jiang Hongju He Hanjun Ma Fusheng Chen Baocheng Xu Hong Liu Mingming Zhu Zhuangli Kang Shengming Zhao | 2021 | International Journal of Agricultural and Biological Engineering2021，14，1： | 2 |
| 5 | Mining hyperspectral data for non-destructive and rapid prediction of nitrite content in ham sausages显示文摘Accurate and rapid determination of nitrite contents is an important step for guaranteeing sausage quality.This study attempted to mine hyperspectral data in the range of 900-1700 nm for non-destructive and rapid prediction of nitrite contents in sausages.The average spectra of 156 samples were collected to relate to the measured nitrite values by partial least squares(PLS)regression.Optimal wavelengths were respectively selected by successive projections algorithm(SPA)and regression coefficients(RC)to simplify the PLS model.The results indicated that PLS model established with 15 optimal wavelengths(900.5 nm,907.1 nm,908.8 nm,912.1 nm,915.4 nm,920.3 nm,922.0 nm,941.7 nm,979.6 nm,1083.2 nm,1213.2 nm,1353.0 nm,1460.2 nm,1595.6 nm and 1699.9 nm)selected by SPA had better performance with r C,r CV,r P of 0.92,0.89,0.89 and RMSEC,RMSECV,RMSEP of 0.41 mg/kg,0.89 mg/kg,0.49 mg/kg,respectively,for calibration set,cross-validation and prediction set.It was concluded that hyperspectral data could be mined by PLS&SPA for realizing the rapid evaluation of nitrite content in ham sausages. | Yadong Zhu Hongju He Shengqi Jiang Hanjun Ma Fusheng Chen Baocheng Xu Hong Liu Mingming Zhu Shengming Zhao Zhuangli Kang | 2021 | International Journal of Agricultural and Biological Engineering2021，14，2： | 1 |
| 6 | Influence of residual stress on the load–deflection curve of diaphragm springs for automobile clutches显示文摘 | Yasunori Doman Toru Fujii Kazuya Okubo Hanjun He | 2003 | JSAE Review2003，，2： | 1 |
| 7 | Influence of residual stress on the load–deflection curve of diaphragm springs for automobile clutches显示文摘 | Yasunori Doman Toru Fujii Kazuya Okubo Hanjun He | 2003 | JSAE Review2003，，2： | 1 |
| 8 | Thermal deformation of pressure plates for manual clutches显示文摘 | HE Hanjun OKUBO K TAKEMOTO Y | 2005 | SAE Technical Paper2005，，： | 1 |
| 9 | Influence of several postharvest processing methods on polyphenol oxidase activity and cichoric acid content of Echinacea purpurea roots显示文摘 | Yanli Zhang Tiexin Tang Hanjun He Hong Wu Zhuoyan Hu | 2011 | Industrial Crops & Products2011，，1： | 1 |
| 10 | An 11-bit 200 MS/s subrange SAR ADC with low-cost integrated reference buffer显示文摘This paper presents an 11-bit 200MS/s subrange S AR ADC with an integrated reference buffer in 65nm CMOS.The proposed ADC employs a 3.5-bit flash ADC for coarse conversion,and a compact timing scheme at the flash/SAR boundary to speed up the conversion.The flash decision is used to control charge compensating for the reference voltage to reduce its input-dependent fluctuation.Measurement results show that the fabricated ADC has achieved significant improvement by applying the reference charge compensation.In addition,the ADC achieves a maximum signal-to-noise-and-distortion ratio of 59.3dB at 200MS/s.It consumes 3.91mW from a 1.2V supply,including the reference buffer. | Xiuju He Xian Gu Weitao Li Hanjun Jiang Fule Li Zhihua Wang | 2017 | Journal of Semiconductors2017，38，10： | 0 |
| 11 | Domain of attraction of quasi-stationary distribution for one-dimensional diffusions显示文摘我们为在 0 点打死的一个维的散开学习 quasi-stationarity,当 0 是一条常规边界, + 是一条入口边界时。我们为存在给一个必要、足够的条件确切,一 quasistationary 分布,和我们也证明这分布吸引所有起始的分布。 | Hanjun ZHANG Guoman HE | 2016 | Frontiers of Mathematics in China2016，11，2： | 0 |
| 12 | Nondestructive determination of IMP content in chilled chicken based on hyperspectral data combined with chemometrics显示文摘This study was conducted to investigate the potential of hyperspectral imaging technique(900-1700 nm)for nondestructive determination of inosinic acid(IMP)in chicken.Hyperspectral images of chicken flesh samples were acquired,and their mean spectra within the images were extracted.The quantitative relationship between the mean spectra and reference IMP value was fitted by partial least squares(PLS)regression algorithm.A PLS model(MAS-PLS)built with moving average smoothing(MAS)spectra showed better performance in predicting IMP content,leading to correlation coefficients(RP)of 0.951,root mean square error(RMSEP)of 0.046 mg/g,and residual predictive deviation(RPD)of 3.152.Regression coefficient(RC),successive projections algorithm(SPA),stepwise,competitive adaptive reweighted sampling(CARS),and uninformative variable elimination(UVE)were used to select the optimal wavelengths to optimize the MAS-PLS model.Based on the 18 optimal wavelengths(907.14,917.02,918.67,926.90,930.20,936.78,956.54,1004.28,1135.89,1211.56,1302.07,1367.94,1397.60,1488.31,1680.17,1683.49,1686.80 and 1695.10 nm)selected from MAS spectra by SPA,the MAS-SPA-PLS model was built with R_(P) of 0.920,RMSEP of 0.056 mg/g and RPD of 3.220,which was similar to the MAS-PLS model.The overall study indicated that hyperspectral imaging in the 900-1700 nm range combined with PLS and SPA could be used to predict the IMP content in chicken flesh. | Yangyang Wang Hongju He Shengqi Jiang Hanjun Ma | 2022 | International Journal of Agricultural and Biological Engineering2022，15，1： | 0 |
| 13 | Irisin Attenuates Osteoarthritis by Inhibiting Apoptosis of Osteocytes Through Activating Erk Signaling Pathway显示文摘Osteoarthritis(OA)is an inflammatory disease involving the joints that is prevalent in the global aging population.The purpose of this study is to determine whether irisin can attenuate osteoarthritis(OA)progression in anterior cruciate ligament transection(ACLT)mice models and the mechanism of irisin therapy effect on OA by increase the resistance of apoptosis in MLO-Y4 cells induced by mechanical stretch in vitro.Methods For in vivo study,3-month-old male C57BL/6 J mice were randomized to three groups,sham-operated,anterior cruciate ligament transection(ACLT)-operated treated with vehicle,and ACLT-operated treated with irisin by intraperitoneal injection once a week.Cartilage erosion was observed by HE staining.Osteoarthritis Research Society International(OARSI)scores were evaluated according to the safranin O stai-ning.The microstructure of tibia cortical bone,trabecular bone,and subchondral bone was analyzed by micro-CT and the bone histomorphometry has been administrated including mineral apposition rate(MAR).Edu staining and cck-8 were used for the detection of the proliferation of MLO-Y4 cells.For mechanical stress,cells were seeded on the collagen-I coated chamber subjected with a peak biaxial stretch of 20%at 1 Hz for 16 hours to induce apoptosis.Flow cytometry was used for the detection of apoptosis and cell cycle.TUNNEL was used for staining the apoptotic cells and rt-PCR was applied for quantifying the expression of mRNA such as Bax,Bcl-2,SOST,c-myc,Opg.Western blot was utilized to confirm the mechanism of how irisin decrease the osteocyte apoptosis.Results In vivo,irisin can attenuate articular cartilage degeneration.Irisin maintains the proportion of hyaline cartilage and calcified cartilage and keep fewer cartilage erosions in ACLT-operated mice.For immunohistochemical(IHC)staining,irisin reduced the expression of caspase3,Bax and matrix metalloproteinase-13 in both cartilage and subchondral bone.Irisin-treated ACLT group shows higher Trabecular number(Tb.N)and bone volume fraction(BV/TV)compared to the vehicle-treated ACLT group.In vitro, irisin significantly increased the proliferation of MLO-Y4 cells detected by Edu and Ki67 staining,and irisin can protect the cells from both mechanical stretchinduced apoptosis detected by FITC-PI flow cytometry and maintain the cell activity by regulating the expression of Bax,Bcl-2,and c-myc.Transcriptome sequencing shows that irisin significantly activates the MAPK signaling pathway and we confirm the result by western blot:irisin effectively activates the Erk signaling pathway through phosphorylation and has a certain activation effect on p38 signaling pathway,no activation was observed for FAK signaling pathway.Conclusions Irisin can attenuate the progression of OA by decrease the apoptosis of osteocyte,which can improve the microarchitecture of subchondral bone.Erk pathway activation plays an important role in reducing the apoptosis of osteocyte. | Zihao He Hanjun Li Feng Zhou Jingke Du Shuhong Zhang Tingting Tang Zhifeng Yu | 2019 | 医用生物力学2019，34，A01： | 0 |
| 14 | Synthesis,microstructure,and formation mechanism of a potential neutron shielding material:WAlB显示文摘The recent re-discovery of nano-laminated,ternary transition metal borides called‘MAB’phases(M=transition metal,A=Al,and B=B)has allowed for an alternative candidate material for certain high-performance applications[1,2].The discovery of de-sirable MAB phase properties including near-room temperature magnetocaloric effects of Fe 2 AlB 2[3],high-temperature oxidation resistance of MoAlB[4],and single-layer 2D molybdenum boride sheets obtained through the chemical exfoliation of laminated compounds has resulted in an increase in research into their syn-thesis and properties[5]. | Dongya Zhang Peter Richardson Meng Wang Hanjun Tu Cong Li Linping He Haibin Zhang Liqun Shi | 2022 | Journal of Materials Science & Technology2022，，31： | 0 |
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