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| 1 | aunalysis of the Genome Sequence of the Medicinal Plant Salvia miltiorrhiza显示文摘 | Haibin Xu Jingyuan Song Hongmei Luo Yujun Zhang Qiushi Li Yingjie Zhu Jiang Xu Ying Li Chi Song Bo Wang Wei Sun Guoan Shen Xin Zhang Jun Qian Aijia Ji Zhichao Xu Xiang Luo Liu He Chuyuan Li Chao Sun Haixia Yah Guanghong Cui Xiwen Li Xian 'en Li Jianhe Wei Juyan Liu Yitao Wang Alice Hayward David Nelson Zemin Ning Reuben J. Peters Xiaoquan Qi Shilin Chen | 2016 | Molecular Plant2016，9，6： | 70 |
| 2 | Enhanced secondary pollution offset reduction of primary emissions during COVID-19 lockdown in China显示文摘To control the spread of the 2019 novel coronavirus(COVID-19),China imposed nationwide restrictions on the movement of its population(lockdown)after the Chinese New Year of 2020,leading to large reductions in economic activities and associated emissions.Despite such large decreases in primary pollution,there were nonetheless several periods of heavy haze pollution in eastern China,raising questions about the well-established relationship between human activities and air quality.Here,using comprehensive measurements and modeling,we show that the haze during the COVID lockdown was driven by enhancements of secondary pollution.In particular,large decreases in NOx emissions from transportation increased ozone and nighttime NO_(3)radical formation,and these increases in atmospheric oxidizing capacity in turn facilitated the formation of secondary particulate matter.Our results,afforded by the tragic natural experiment of the COVID-19 pandemic,indicate that haze mitigation depends upon a coordinated and balanced strategy for controlling multiple pollutants. | Xin Huang Aijun Ding Jian Gao Bo Zheng Derong Zhou Ximeng Qi Rong Tang Jiaping Wang Chuanhua Ren Wei Nie Xuguang Chi Zheng Xu Liangduo Chen Yuanyuan Li Fei Che Nini Pang Haikun Wang Dan Tong Wei Qin Wei Cheng Weijing Liu Qingyan Fu Baoxian Liu Fahe Chai Steven JDavis Qiang Zhang Kebin He | 2021 | National Science Review2021，8，2： | 47 |
| 3 | OsCERK1-Mediated Chitin Perception and Immune Signaling Requires Receptor-like Cytoplasmic Kinase 185 to Activate an MAPK Cascade in Rice显示文摘保存联系病原体的分子的模式(PAMP ) 例如几丁质,被在激活 mitogen 的蛋白质 kinase (MAPK ) 的快速的激活串联的宿主细胞表面和扳机定位的模式识别受体(PRR ) 察觉,它为植物抵抗被要求到病原体。然而,到在植物的 MAPK 激活的从 PAMP 感觉的直接连接仍然保持大部分未知。在这研究,我们发现联系 PRR 的像受体的细胞质的 kinase Oryza sativa RLCK185 播送到通过和 MAPK kinase kinase 的相互作用表明串联的 MAPK 的从 PAMP 受体 OsCERK1 的有免疫力的发信号, OsMAPKKK,它是 MAPK 串联的起始的 kinase。OsRLCK185 交往与并且 phosphorylates OsMAPKKK 的 C 终端规章的领域。phosphomimetic OsRLCK185 和 OsMAPKKK 的 Coexpression 在 Nicotiana benthamiana 叶子激活 MAPK3/6 phosphorylation。而且, OsMAPKKK 交往与并且 phosphorylates OsMKK4,关键 MAPK kinase 那 transduces 几丁质信号。OsMAPKKK 的 Overexpression 增加导致几丁质的 MAPK3/6 激活,而 OsMAPKKK 击倒的妥协导致几丁质的 MAPK3/6 激活和抵抗到米饭强风真菌。一起拿,我们的结果在米饭从房间表面几丁质感觉建议一条发信号 phospho 小径的存在到 MAPK 串联的细胞内部的激活。 | Chao Wang Gang Wang Chi Zhang Pinkuan Zhu Huiling Dai Nan Yu Zuhua He Ling Xu Ertao Wang | 2017 | Molecular Plant2017，10，4： | 27 |
| 4 | Swelling of clay minerals in ammonium leaching of weathered crust elution-deposited rare earth ores显示文摘The effect of hydrated radius, cation valence, pH and solution concentration on the zeta potential of clay minerals was investigated, and the relation between zeta potential of clay minerals and swelling was discussed in different leaching agents as well. The results show that the zeta potential of clay minerals decreases with the hydrated ionic radius increasing. It could be seen that the zeta potential of the clay minerals in AICl_3 solution is positive,whereas that in NH_4C1, KCl and MgCl_2 solution is negative. And the zeta potential of clay minerals increases with the cation valence increasing. Moreover, the zeta potential of clay minerals decreases with the solution pH increasing,whereas that increases with the solution concentration increasing in different ammonium solutions. In addition,the swelling of clay minerals decreases while the zeta potential of clay minerals increasing in different ammonium solutions. The ability of compound ammonium to inhibit the swelling of clay minerals is lower than that of single ammonium solution. | Zhen-Yue Zhang Zheng-Yan He Fang Zhou Cheng-Bin Zhong Ning-Jie Sun Ru-An Chi | 2018 | Rare Metals2018，37，1： | 15 |
| 5 | Effects of dietary fiber on human health显示文摘Dietary fiber(DF)is believed to provide important health benefits and it has become a research hotspot.DF exists in most natural foods,including fruits(16.74%-91.24%),vegetables(6.53%-85.19%),grains(9.76%-69.20%)and so on.DF has different physicochemical properties such as water holding capacity(WHC)(2.01-25.03 g/g),water swelling capacity(WSC)(0.95-23.90 mL/g),oil holding capacity(OHC)(0.65-29.00 g/g),glucose adsorption capacity(GAC)(0.17-4.65 mmol/g),cholesterol adsorption capacity(CAC)(0.03-37.10 mg/g)and viscosity,which make it exhibits different physiological functions such as reducing the risk of obesity,diabetes,cancer,and intestinal diseases.On the basis of consulting related databases,the physicochemical properties of DF and its derived physiological functions are reviewed.It is found that DF has effect on the prevention and treatment of obesity due to good WHC,WSC,OHC and CAC.Good GAC can relieve the symptoms of diabetes.The physicochemical properties of WHC and WSC can reduce the risk of cancer.The WHC,WSC and viscosity are beneficial to intestinal health. | Yang He Bixiang Wang Liankui Wen Fengzhong Wang Hansong Yu Dongxia Chen Xin Su Chi Zhang | 2022 | Food Science and Human Wellness2022，11，1： | 15 |
| 6 | Prostate cancer in Asia:A collaborative report显示文摘The incidence of prostate cancer(PCa)within Asian population used to be much lower than in the Western population;however,in recent years the incidence and mortality rate of PCa in some Asian countries have grown rapidly.This collaborative report summarized the latest epidemiology information,risk factors,and racial differences in PCa diagnosis,current status and new trends in surgery management and novel agents for castration-resistant prostate cancer.We believe such information would be helpful in clinical decision making for urologists and oncologists,health-care ministries and medical researchers. | Rui Chen Shancheng Ren 无 Ming Kwong Yiu Ng Chi Fai Wai Sam Cheng Lap Hong Ian Seiji Naito Tadashi Matsuda Elijah Kehinde Ali Kural Jason Yichun Chiu Rainy Umbas Qiang Wei Xiaolei Shi Liqun Zhou Jian Huang Yiran Huang Liping Xie Lulin Ma Changjun Yin Danfeng Xu Kexin Xu Zhangqun Ye Chunxiao Liu Dingwei Ye Xin Gao Qiang Fu Jianquan Hou Jianlin Yuan Dalin He Tiejun Pan Qiang Ding Fengshuo Jin Benkang Shi Gongxian Wang Xiuheng Liu Dongwen Wang Zhoujun Shen Xiangbo Kong Wanhai Xu Yaoliang Deng Haibo Xia Alexa NCohen Xu Gao Chuanliang Xu Yinghao Sun | 2014 | Asian Journal of Urology2014，1，1： | 13 |
| 7 | Local concentration of middle and heavy rare earth elements in the col on the weathered crust elution-deposited rare earth ores显示文摘This present study focused on investigating the content and relative enrichment multiple of rare earth elements(REEs) in different terrain of weathered ore body, and the value of light rare earth elements/middle and heavy rare earth elements(LREEs/HREEs), in order to understand the mobilization and redistribution of HREEs, Furthermore, the abrasion pH was investigated as well, The results show that the average contents of HREEs in the knap, ridge and col are 37.26, 61.71, and 271.3 μg/g, respectively. The value of LREEs/HREEs is decreased from 16.78 to 7.914 between knap and col and the relative enrichment multiple of HREEs is as follows: C_(Col-HREEs) > C_(Ridge-HREEs) > 0. It is indicated the HREEs is concentrated in the col and the enrichment degree of HREEs is stronger than the ridge and the knap. Rare earth elements fractionation is exhibited in the different terrain of weathered ore body. Based on this study, the finding that HREEs concentrate in the lower terrain of weathered crust elution-deposited rare earth ores, such as in the col, would become a marker or indicator to search for middle and heavy rare earth enriched zone. | Zhenyue Zhang Ningjie Sun Zhengyan He Ru'an Chi | 2018 | Journal of Rare Earths2018，36，5： | 10 |
| 8 | ATF3 contributes to brucine-triggered glioma cell ferroptosis via promotion of hydrogen peroxide and iron显示文摘Ferroptotic cell death is characterized by iron-dependent lipid peroxidation that is initiated by ferrous iron and H_(2)O_(2) via Fenton reaction,in which the role of activating transcription factor 3(ATF3)remains elusive.Brucine is a weak alkaline indole alkaloid extracted from the seeds of Strychnos nux-vomica,which has shown potent antitumor activity against various tumors,including glioma.In this study,we showed that brucine inhibited glioma cell growth in vitro and in vivo,which was paralleled by nuclear translocation of ATF3,lipid peroxidation,and increases of iron and H_(2)O_(2).Furthermore,brucine-induced lipid peroxidation was inhibited or exacerbated when intracellular iron was chelated by deferoxamine(500μM)or improved by ferric ammonium citrate(500μM).Suppression of lipid peroxidation with lipophilic antioxidants ferrostatin-1(50μM)or liproxstatin-1(30μM)rescued brucine-induced glioma cell death.Moreover,knockdown of ATF3 prevented brucine-induced accumulation of iron and H_(2)O_(2) and glioma cell death.We revealed that brucine induced ATF3 upregulation and translocation into nuclei via activation of ER stress.ATF3 promoted brucine-induced H_(2)O_(2) accumulation via upregulating NOX4 and SOD1 to generate H_(2)O_(2) on one hand,and downregulating catalase and xCT to prevent H_(2)O_(2) degradation on the other hand.H_(2)O_(2) then contributed to brucine-triggered iron increase and transferrin receptor upregulation,as well as lipid peroxidation.This was further verified by treating glioma cells with exogenous H_(2)O_(2) alone.Moreover,H_(2)O_(2) reversely exacerbated brucine-induced ER stress.Taken together,ATF3 contributes to brucine-induced glioma cell ferroptosis via increasing H_(2)O_(2) and iron. | Shan Lu Xuan-zhong Wang Chuan He Lei Wang Shi-peng Liang Chong-cheng Wang Chen Li Tian-fei Luo Chun-sheng Feng Zhen-chuan Wang Guang-fan Chi Peng-fei Ge | 2021 | Acta Pharmacologica Sinica2021，42，10： | 10 |
| 9 | Identification of a Resistance Gene bls1 to Bacterial Leaf Streak in Wild Rice Oryza rufipogon Griff显示文摘Bacterial leaf streak (BLS) of rice, caused by Xanthomonas oryzae pv. oryzicola (Xoc) is a worldwide destructive disease. Development of resistant varieties is considered to be one of the most effective and eco-friendly ways to control the disease. However, only a few genes/QTLs having resistance to BLS have been identified in rice until now. In the present study, we have identified and primarily mapped a BLS-resistance gene, bls1, from a rice line DP3, derived from the wild rice species Oryza rufipogon Griff. A BC2F2 (9311/DP3//9311) population was constructed to map BLS-resistance gene in the rice line DP3. The segregation of the resistant and susceptible plants in BC2F2 in 1:3 ratio (c2=0.009, c20.05, 1=3.84, P>0.05), suggested that a recessive gene confers BLS resistance in DP3. In bulked segregant analysis (BSA), two SSR markers RM8116 and RM584 were identified to be polymorphic in resistant and susceptible DNA bulks. For further mapping the resistance gene, six polymorphic markers around the target region were applied to analyze the genotypes of the BC2F2 individuals. As a result, the BLS-resistant gene, designated as bls1, was mapped in a 4.0-cM region flanked by RM587 and RM510 on chromosome 6. | HE Wen-ai HUANG Da-hui LI Rong-bai QIU Yong-fu SONG Jian-dong YANG Hai-ning ZHENG Jia-xing HUANG Yue-yue LI Xiao-qiong LIU Chi ZHANG Yue-xiong MA Zeng-feng YANG Yong | 2012 | Journal of Integrative Agriculture2012，11，6： | 9 |
| 10 | Catalytic oxidation of benzene over nanostructured porous Co_3O_4-CeO_2 composite catalysts显示文摘Mesostructured Co3O4-CeO2 composite was found to be an effective catalytic material for the complete oxidation of benzene. The Co3O4-CeO2 catalysts with different Co/Ce ratios (mol/mol) were prepared via the nanocasting method and the mesostructure was replicated from two-dimensional (2D) hexagonal SBA-15 and three-dimensional (3D) cubic KIT-6 silicas, respectively. All the obtained Co3O4-CeO2 catalysts exhibited the similar symmetry with the parent silicas and well ordered mesostructures. The Co3O4- CeO2 catalysts with 2D mesostructure showed lower catalytic activities than the corresponding 3D materials. The Co3O4-CeO2 catalyst nanocasted from KIT-6 and with the Co/Ce ratio of 16/1 possessed the best catalytic benzene oxidation activity due to larger quantities of surface hydroxyl groups and surface oxygenated species. The mesostructured Co3O4-CeO2 material thus shows great potential as a promising eco-environmental catalyst for benzene effective elimination. | Chunyan Ma Zhen Mu Chi He Peng Li Jinjun Li Zhengping Hao | 2011 | Journal of Environmental Sciences2011，23，12： | 9 |
| 11 | Olivine-norite rock detected by the lunar rover Yutu-2 likely crystallized from the SPA-impact melt pool显示文摘Chang’E-4 landed in the South Pole-Aitken(SPA)basin,providing a unique chance to probe the composition of the lunar interior.Its landing site is located on ejecta strips in Von Karm´an crater that´possibly originate from the neighboring Finsen crater.A surface rock and the lunar regolith at 10 sites along the rover Yutu-2 track were measured by the onboard Visible and Near-Infrared Imaging Spectrometer in the first three lunar days of mission operations.In situ spectra of the regolith have peak band positions at 1 and 2μm,similar to the spectral data of Finsen ejecta from the Moon Mineralogy Mapper,which confirms that the regolith’s composition of the landing area is mostly similar to that of Finsen ejecta.The rock spectrum shows similar band peak positions,but stronger absorptions,suggesting relatively fresh exposure.The rock may consist of 38.1±5.4%low-Ca pyroxene,13.9±5.1%olivine and 48.0±3.1%plagioclase,referred to as olivine-norite.The plagioclase-abundant and olivine-poor modal composition of the rock is inconsistent with the origin of the mantle,but representative of the lunar lower crust.Alternatively,the rock crystallized from the impact-derived melt pool formed by the SPA-impact event via mixing the lunar crust and mantle materials.This scenario is consistent with fast-cooling thermal conditions of a shallow melt pool,indicated by the fine to medium-sized texture(<3 mm)of the rock and the SPA-impact melting model[Icarus 2012;220:730–43]. | Honglei Lin Zhiping He Wei Yang Yangting Lin Rui Xu Chi Zhang Meng-Hua Zhu Rui Chang Jinhai Zhang Chunlai Li Hongyu Lin Yang Liu Sheng Gou Yong Wei Sen Hu Changbin Xue Jianfeng Yang Jie Zhong Xiaohui Fu Weixing Wan Yongliao Zou | 2020 | National Science Review2020，7，5： | 9 |
| 12 | A LysM Receptor Heteromer Mediates Perception of Arbuscular Mycorrhizal Symbiotic Signal in Rice显示文摘Symbiotic microorganisms improve nutrient uptake by plants.To initiate mutualistic symbiosis with arbus-cular mycorrhizal(AM)fungi,plants perceive Myc factors,including lipochitooligosaccharides(LCOs)and short-chain chitooligosaccharides(CO4/CO5),secreted by AM fungi.However,the molecular mechanism of Myc factor perception remains elusive.In this study,we identified a heteromer of LysM receptor-like kinases consisting of OsMYR1/OsLYK2 and OsCERK1 that mediates the perception of AM fungi in rice.CO4 directly binds to OsMYR1,promoting the dimerization and phosphorylation of this receptor complex.Compared with control plants,Osmyr1 and Oscerk1 mutant rice plants are less sensitive to Myc factors and show decreased AM colonization.We engineered transgenic rice by expressing chimeric receptors that respectively replaced the ectodomains of OsMYR1 and OsCERK1 with those from the homologous Nod factor receptors MtNFP and MtL YK3 of Medicago truncatula.Transgenic plants displayed increased cal-cium oscillations in response to Nod factors compared with control rice.Our study provides significant mechanistic insights into AM symbiotic signal perception in rice.Expression of chimeric Nod/Myc recep-tors achieves a potentially important step toward generating cereals that host nitrogen-fixing bacteria. | Jiangman He Chi Zhang Huiling Dai Huan Liu Xiaowei Zhang Jun Yang Xi Chen Yayun Zhu Dapeng Wang Xiaofeng Qi Weichao Li Zhihui Wang Guoyong An Nan YU Zuhua He Yong-Fei Wang Youli Xiao Peng Zhang Ertao Wang | 2019 | Molecular Plant2019，12，12： | 9 |
| 13 | A plasmonic route for the integrated wireless communication of subdiffraction-limited signals显示文摘Perfect lenses,superlenses and time-reversal mirrors can support and spatially separate evanescent waves,which is the basis for detecting subwavelength information in the far field.However,the inherent limitations of these methods have prevented the development of systems to dynamically distinguish subdiffraction-limited signals.Utilizing the physical merits of spoof surface plasmon polaritons(SPPs),we demonstrate that subdiffraction-limited signals can be transmitted on planar integrated SPP channels with low loss,low channel interference,and high gain and can be radiated with a very low environmental sensitivity.Furthermore,we show how deep subdiffraction-limited signals that are spatially coupled can be distinguished after line-of-sight wireless transmission.For a visualized demonstration,we realize the high-quality wireless communication of two movies on subwavelength channels over the line of sight in real time using our plasmonic scheme,showing significant advantages over the conventional methods. | Hao Chi Zhang Le Peng Zhang Pei Hang He Jie Xu Cheng Qian Francisco J.Garcia-Vidal Tie Jun Cui | 2020 | Light(Science & Applications)2020，9，1： | 8 |
| 14 | KNaY-zeolite catalyzed dehydration of methyl lactate显示文摘A promising catalyst,KNaY was prepared by an ion exchange method with aqueous potassium chloride solution.Comparedwith NaY,KNaY was an effective catalyst for the dehydration of methyl lactate to methyl acrylate.Under the optimized conditions,an improved yield of 37.9 mol%was achieved. | Hai Feng Shi Yao Chi Hu Yang Wang He Huang | 2007 | Chinese Chemical Letters2007，18，4： | 8 |
| 15 | Experimental study on MSW gasification and melting technology显示文摘以便开发市政的稳固的浪费(MSW ) 气化和融化的技术,二预备试验和一个原则综合实验分别地被完成。当时, MSW 的气化特征在 500 750 ° C 被学习等价比率(嗯) 是 0.2 0.5 使用使流体化床的 gasifier。当温度是 550 700 ° C 时并且嗯是 0.2 0.4, syngas 的低热价值(LHV ) 到达 4000 12000 kJ/Nm3。苍蝇灰的融化的特征用一个改正床炉子在 1100 1460 ° C 被调查。超过 99.9% 戴奥辛能被分解,这被证明并且很重金属当温度是 1100 1300 ° C 时,能被团结。原则综合实验在使流体化床的气化和打漩融化系统被执行。MSW 在 550 650 ° C 高效地被气化,打漩融化炉子稳定地并且过去在 1200 1300 ° C 坚持说 95% 苍蝇灰能被打漩融化炉子抓住。结果提供了实际经验和基本数据开发 MSW 气化和融化的技术。 | XIAO Gang JIN Bao-sheng ZHONG Zhao-ping CHI Yong NI Ming-jiang CEN Ke-fa XIAO Rui HUANG Ya-ji HUANG He | 2007 | Journal of Environmental Sciences2007，19，11： | 7 |
| 16 | SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target显示文摘Cytokine storm and multi-organ failure are the main causes of SARS-CoV-2-related death.However,the origin of excessive damages caused by SARS-CoV-2 remains largely unknown.Here we show that the SARS-CoV-2 envelope(2-E)protein alone is able to cause acute respiratory distress syndrome(ARDS)-like damages in vitro and in vivo. | Bingqing Xia Xurui Shen Yang He Xiaoyan Pan Feng-Liang Liu Yi Wang Feipu Yang Sui Fang Yan Wu Zilei Duan Xiaoli Zuo Zhuqing Xie Xiangrui Jiang Ling Xu Hao Chi Shuangqu Li Qian Meng Hu Zhou Yubo Zhou Xi Cheng Xiaoming Xin Lin Jin Hai-Lin Zhang Dan-Dan Yu Ming-Hua Li Xiao-Li Feng Jiekai Chen Hualiang Jiang Gengfu Xiao Yong-Tang Zheng Lei-Ke Zhang Jingshan Shen Jia Li Zhaobing Gao | 2021 | Cell Research2021，31，8： | 7 |
| 17 | FOXO3a protects glioma cells against temozolomide-induced DNA double strand breaks via promotion of BNIP3-mediated mitophagy显示文摘FOXO3a(forkhead box transcription factor 3a)is involved in regulating multiple biological processes in cancer cells.BNIP3(Bcl-2/adenovirus E1B 19-kDa-interacting protein 3)is a receptor accounting for priming damaged mitochondria for autophagic removal.In this study we investigated the role of FOXO3a in regulating the sensitivity of glioma cells to temozolomide(TMZ)and its relationship with BNIP3-mediated mitophagy.We showed that TMZ dosage-dependently inhibited the viability of human U87,U251,T98G,LN18 and rat C6 glioma cells with IC_(50) values of 135.75,128.26,142.65,155.73 and 111.60μM,respectively.In U87 and U251 cells,TMZ(200μM)induced DNA double strand breaks(DSBs)and nuclear translocation of apoptosis inducing factor(AIF),which was accompanied by BNIP3-mediated mitophagy and FOXO3a accumulation in nucleus.TMZ treatment induced intracellular ROS accumulation in U87 and U251 cells via enhancing mitochondrial superoxide,which not only contributed to DNA DSBs and exacerbated mitochondrial dysfunction,but also upregulated FOXO3a expression.Knockdown of FOXO3a aggravated TMZ-induced DNA DSBs and mitochondrial damage,as well as glioma cell death.TMZ treatment not only upregulated BNIP3 and activated autophagy,but also triggered mitophagy by prompting BNIP3 translocation to mitochondria and reinforcing BNIP3 interaction with LC3BII.Inhibition of mitophagy by knocking down BNIP3 with SiRNA or blocking autophagy with 3MA or bafilomycin A1 exacerbated mitochondrial superoxide and intracellular ROS accumulation.Moreover,FOXO3a knockdown inhibited TMZ-induced BNIP3 upregulation and autophagy activation.In addition,we showed that treatment with TMZ(100 mg·kg^(-1)·d^(-1),ip)for 12 days in C6 cell xenograft mice markedly inhibited tumor growth accompanied by inducing FOXO3a upregulation,oxidative stress and BNIP3-mediated mitophagy in tumor tissues.These results demonstrate that FOXO3a attenuates temozolomide-induced DNA double strand breaks in human glioma cells via promoting BNIP3-mediated mitophagy. | Chuan He Shan Lu Xuan-zhong Wang Chong-cheng Wang Lei Wang Shi-peng Liang Tian-fei Luo Zhen-chuan Wang Mei-hua Piao Guang-fan Chi Peng-fei Ge | 2021 | Acta Pharmacologica Sinica2021，42，8： | 7 |
| 18 | IL-6 and INF-γ levels in patients with brucellosis in severe epidemic region,Xinjiang,China显示文摘Background:The incidence of brucellosis,which is caused by the Brucella species of bacteria,is rapidly rising worldwide;however,few studies have investigated the immune response to this pathogen and clinical biochemical features.In this paper,we examined the levels of various cytokines and inflammatory factors as well as clinical course characteristics in patients with brucellosis,in order to provide evidence for the diagnosis,assessment,and prognosis of this infectious disease.Methods:A total of 191 brucellosis inpatients(50 acute cases and 141 chronic cases),as well as 60 healthy control subjects,were included in the analysis.We investigated changes in the levels of six cytokines(IL-4,IL-6,IL-10,IL-17,TNF-α,INF-γ)and related clinical biochemical markers in patients with acute and chronic brucellosis in Xinjiang,China.Possible factors were statistically analyzed using the t test,χ2 test,z test and a multivariate logistic stepwise regression test.Results:We found that IL-4,IL-6,IL-10,IL-17,IFN-γ,and TNF-αlevels were higher in those with brucellosis than in controls(P<0.05).With regard to disease progression,procalcitonin(PCT)and C-reactive protein(CRP)levels were significantly higher in those with an acute infection compared to chronic cases(P<0.05).We found that the expression of all six cytokines tested was closely related to the degree of brucellosis using univariate logistic regression;however,only IL-6 and INF-γlevels were independent factors associated with the severity of brucellosis.Conclusions:Assessing cytokine levels in patients with acute and chronic brucellosis is not only useful for detecting the immune response,but can also be indicative of the severity of brucellosis.In particular,we propose IL-6 and INF-γlevels may be useful independent predictive factors in the clinical evaluation and diagnosis of brucellosis. | Zhi-Qiang Lin Guo-Yue Lin Wen-Wen He Chi Zhang Rui Zhang Yuan-Da Li Fan Wang Ying Qin Li Duan Dou-Dou Zhao Xiao-Juan Qu Hui Gao Hai Jiang | 2020 | Infectious Diseases of Poverty2020，9，3： | 7 |
| 19 | Antigenically dominant proteins within the human liver mitochondrial proteome identified by monoclonal antibodies显示文摘Analysis of the mitochondrial proteome would provide valuable insight into the function of this important organelle, which plays key roles in energy metabolism, apoptosis, free radical production, thermogenesis, and calcium signaling. It could also increase our understanding about the mechanisms that promote mitochondrial disease. To identify proteins that are antigenically dominant in human liver mitochondria, we generated >240 hybridoma cell lines from native mitochondrial proteins after cell fusion, screening, and cloning. Antibodies that recognized mitochondrial proteins were identified by screening human liver cDNA expression libraries. In this study, we identified 6 major antigens that were recognized by at least 2 different monoclonal antibodies (mAbs). The proteins that were antigenically dominant were: acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase), aldehyde dehydrogenase 1 family member A1, carbamoyl phosphate synthetase 1, dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex), enoyl coenzyme A hydratase 1, and hydroxysteroid (11-beta) dehydrogenase 1. We also determined the subcellular localizations of these enzymes within the mitochondria using immunohistocytochemistry. We believe that these well-characterized antibodies will provide a valuable resource for the Human Liver Proteome Project (HLPP), and will make studies aimed at investigating liver mitochondrial function far easier to perform in future. Our results provide strong evidence that, (i) depletion of dominant proteins from liver mitochondrial samples is possible and, (ii) the approaches adopted in this study can be used to explore or validate protein-protein interactions in this important organelle. | JU YanFang YANG JinJu LIU Rong LIU XiaoLan DU XueMei LIU Li CHEN ZhiCheng CHI Jun LIU ShuEr GAO Yuan GAO JianEn JIAO ShunChang HE FuChu SUN QiHong | 2011 | Science China(Life Sciences)2011，54，1： | 6 |
| 20 | Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen-use efficiency in the wheat cultivar Kenong 9204显示文摘Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and metabolism.By comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in Triticeae.Further studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats.To explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen conditions.Compared with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and seeds.Transcriptome data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in KN9204.In addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed maturity.Physiological and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive stage.The high NUE of KN9204 is an integrated effect controlled at different levels.Taken together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait. | Xiaoli Shi Fa Cui Xinyin Han Yilin He Long Zhao Na Zhang Hao Zhang Haidong Zhu Zhexin Liu Bin Ma Shusong Zheng Wei Zhang Jiajia Liu Xiaoli Fan Yaoqi Si Shuiquan Tian Jianqing Niu Huilan Wu Xuemei Liu Zhuo Chen Deyuan Meng Xiaoyan Wang Liqiang Song Lijing Sun Jie Han Hui Zhao Jun Ji Zhiguo Wang Xiaoyu He Ruilin Li Xuebin Chi Chengzhi Liang Beifang Niu Jun Xiao Junming Li Hong-Qing Ling | 2022 | Molecular Plant2022，15，9： | 6 |
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