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| 1 | Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA显示文摘光周期敏感、 thermo 敏感的遗传因子的男绝育(PGMS 和 TGMS ) 是为在庄稼的混合繁殖的核心部件。基于使用的较普通活字大一倍的系统, PGMS 和 TGMS 衬里的混合大米是成功地在农业广泛地发展了并且适用。然而,位于 PGMS 和 TGMS 的控制下面的分子的机制仍然保持阴暗。在这研究,我们印射并且克隆一个主要地点, p/tms12-1 (染色体 12 上的感光性或 thermo 敏感的遗传因子的男绝育地点) 它在装饰用的梨树米饭线 Nongken 58S (NK58S ) 授与 PGMS 和在 indica 米饭的 TGMS 衬里 Peiai 64S (PA64S,源于 NK58S ) 。包含野类型的等位基因 P/TMS12-1 的 A 2.4-kb DNA 碎片能在基因互补恢复 NK58S 和 PA64S 植物的花粉富饶。P/TMS12-1 编码唯一的 noncoding RNA,它生产 21 核苷酸我们说出 osa-smR5864w 的小 RNA。在 p/tms12-1 的 C-to-G 的替换,相对 P/TMS12-1 的唯一的多型性,在变异的小 RNA 是在场的,也就是 osa-smR5864m。而且,在转基因的 NK58S 和 PA64S 植物的 P/TMS12-1 的一个 375-bp 序列的 overexpression 也生产了 osa-smR5864w 并且恢复了花粉富饶。小 RNA 在年轻圆锥花序优先地被表示,但是它的表示没被不同的天长度或温度显著地影响。我们的结果表明在 p/tms12-1 的点变化,可能为 osa-smR5864m 导致 loss-of-function,分别地在装饰用的梨树和 indica 线为 PGMS 和 TGMS 组成一个普通原因。我们的调查结果因此建议那小 RNA 基因是的这 noncoding 男开发的一个重要管理者由在基因网络和环境条件之间的串音控制了。 | Hai Zhou Qinjian Liu Jing Li Dagang Jiang Lingyan Zhou Ping Wu Sen Lu Feng Li Liya Zhu Zhenlan Liu Letian Chen Yao-Guang Liu Chuxiong Zhuang | 2012 | Cell Research2012，22，4： | 99 |
| 2 | One-step generation of knockout pigs by zygote injection of CRISPR/Cas system显示文摘 | Tang Hai Fei Teng Runfa Guo Wei Li Qi Zhou | 2014 | Cell Research2014，24，3： | 131 |
| 3 | Long-term trend and abrupt events of the Holocene Asian monsoon inferred from a stalagmiteδ^(18)O record from Shennongjia in Central China显示文摘A high-resolution oxygen-isotope record from a thorium-uranium-dated stalagmite from Shanbao Cave at Shennongjia reflects variations in the amount of monsoon precipitation for the period from 11.5 to 2.1 ka (1 ka = 1000 cal aBP). Between 11.5 and 9.3 ka, a sharp decrease in δ18O indicates a rapid increase in monsoon precipitation. An interval of generally high monsoon precipitation is observed between 9.3 and 4.4 ka. An arid period has prevailed between 4.4 and 2.1 ka. The long-term trend of Shanbao record appears to follow summer insolation at 33°N latitude. An abrupt decrease in monsoon precipitation around 4.3 ka is synchronous with the collapse of Neolithic culture in central China. This abrupt change could have resulted from the amplifi- cation of the gradually decreased summer insolation by the positive vegetation-atmosphere-aerosol feed- back. The weakened Asian monsoon events were in concert with decreased Greenland temperature dur- ing the early Holocene, centered at 8.2, 8.6, 9.3, 10.2 and 11.0 ka. This correlation suggests that changes in low-latitude monsoon are connected with climate change in high-latitude polar region. | SHAO Xiaohua WANG Yongjin CHENG Hai KONG Xinggong WU Jiangying EDWARDS R. Lawrence | 2006 | Chinese Science Bulletin2006，51，2： | 64 |
| 4 | Lake eutrophication and its ecosystem response显示文摘China is a country with many lakes,about one-third of which are freshwater mainly distributed in the middle and lower reaches of the Yangtze River.Currently most of the lakes are mesotrophic or eutrophic.Lake eutrophication has become one of the major ecological and environmental problems faced by lakes in China and can lead to a series of abnormal ecosystem responses,including extinction of submerged plants,frequent occurrence of cyanobacterial blooms,increased microbial biomass and productivity,decreased biodiversity,accelerated cycles,and a change in the efficient use of nutrients.With development of eutrophication,the whole lake ecosystem suffers decreased biodiversity,simplification of biotic community structure,instability of the ecosystem,and ultimately the clear-water,macrophyte-dominated ecosystem gradually shifts to a turbid-water,algae-dominated ecosystem.This ecosystem succession mechanism is speculated to be caused by different nutrient utilization efficiencies of macrophytes and phytoplankton.The ultimate ecosystem succession trend of seriously eutrophic lakes is that a phytoplankton-dominated autotrophic lake shifts to a heterotrophic lake dominated by micro-organisms,protozoans. | QIN BoQiang GAO Guang ZHU GuangWei ZHANG YunLin SONG YuZhi TANG XiangMing XU Hai DENG JianMing | 2013 | Chinese Science Bulletin2013，58，9： | 63 |
| 5 | Prognostic significance of expression of cyclooxygenase-2 and vascular endothelial growth factor in human gastric carcinoma显示文摘AIM: To investigate the role of cyclooxygenase-2(COX-2)and vascular endothelial growth factor (VEGF) in the development of gastric carcinoma and correlation between expression of COX-2 and VEGF and clinicopathologic features in tissues from patients with gastric carcinoma.METHODS: 281 patients with gastric carcinoma who underwent surgical resection between 1990 and 1999 at the First Affiliated Hospital, Anhui Medical University, PRC, were followed up. Expression of COX-2 and VEGF was investigated retrospectively in 232 gastric carcinoma tissues and 60noncancerous specimens by using immunohistochemistry.RESULTS: The 5-year survival rates of early gastric carcinoma (EGC) and advanced gastric carcinoma (AGC) were 93.4 %and 59.0 %, respectively. Survival time was highly correlated with lymph node metastasis, vascular invasion, depth of invasion and treatment with chemotherapy. Compared with paired noncancerous tissues, expression of COX-2 and VEGF and microvessel density (MVD) value in carcinoma tissue were significantly higher. The MVD value was much higher in COX-2-positive group and VEGF-positive group than that in COX-2-negative group and VEGF-negative group.Expression of COX-2 and VEGF, as well as MVD value were highly correlated with lymph node metastasis and vascular invasion. The 5-year survival rate of patients with expression of COX-2 or VEGF was significantly lower than that of patients without COX-2 or VEGF expression. Multivariate analysis revealed that VEGF overexpression, lymph node metastasis,COX-2 overexpression, depth of invasion and vascular invasion were all independent prognostic factors of gastric carcinoma.CONCLUSION: Overexpression of COX-2 and VEGF in patients with gastric carcinoma can enhance the possibility of invasion and metastasis, implicating a poor prognosis.They may serve as the fairly good prognostic factors to indicate biologic behaviors of gastric carcinoma. | Hai Shi Jian-Ming Xu Nai-Zhong Hu Hui-Jun Xie Department of Gastroenterology,the First Affiliated Hospital,Anhui Medical University,Hefei 23022,Anhui Province,China | 2003 | World Journal of Gastroenterology2003，9，7： | 64 |
| 6 | 采用PCR-DGGE技术分析蛋鸡肠道细菌种群结构及多样性显示文摘利用基于16S rDNA的PCR-DGGE(变性梯度凝胶电泳)图谱技术结合特异性和共性条带割胶回收DNA进行克隆和测序,对2、4、6和8周龄蛋鸡嗉囊、十二指肠、空肠、回肠和盲肠内容物细菌群落的结构和多样性进行了比较,并鉴定了8周龄蛋鸡部分特异性和共性群落成员,分析蛋鸡年龄和肠段部位对微生物群落结构和多样性的影响。肠道菌群DGGE图谱显示肠道部位对细菌种群结构影响很大,盲肠内微生物的多样性最高,其次是回肠和空肠,嗉囊和十二指肠的微生物多样性比较低。十二指肠内细菌与其它肠段相比差异最大,其次是盲肠与其它肠段细菌组成的差异。随着蛋鸡周龄的增加,消化道微生物经历了由简单(2周)到复杂(4周),再回复简单(6周)到复杂(8周)的变化过程。DGGE图谱中共性条带序列分析表明8周龄蛋鸡消化道前段的优势细菌是三得利乳杆菌(Lactobacillus suntoryeus)、索氏梭菌(Clostridium sordellii)和大肠杆菌,盲肠中的特异性条带主要是各种不可培养的细菌以及超巨巨单胞菌(Megamonas hypermegale)和梭菌属细菌(Clostridiumspp)。该研究结果表明蛋鸡肠道部位决定细菌群落的结构和多样性,蛋鸡的周龄影响肠道细菌多样性。 | 倪学勤 Joshua Gong Hai Yu 曾东 Shayan Sharif 周小秋 | 2008 | 畜牧兽医学报2008，39，7： | 56 |
| 7 | Mobius Isoparametric Hypersurfaces in S^(n+1) with Two Distinct Principal Curvatures | Hai Zhong LI Department of Mathematics, Tsinghua University. Beijing 100084. P. R. China Hui Li LIU Department of Mathematics, Northeastern University. Shenyang 110000. P. R. China Chang Ping WANG Key Laboratory of Pure and Applied Mathematics, School of Mathematical Sciences. Peking University, Beijing 100871, P. R. China Guo Song ZHAO Department of Mathematics, Sichuan University, Chengdu 610064. P. R. China | 2002 | Acta Mathematica Sinica,English Series2002，18，3： | 53 |
| 8 | Identification and genetic mapping of four novel genes that regulate leaf development in Arabidopsis显示文摘Molecular and genetic characterizations of mutants have led to a better understanding of many developmental processes in the model system Arabidopsis thaliana. However, the leaf development that is specific to plants has been little studied. With the aim of contributing to the genetic dissection of leaf development, we have performed a large-scare screening for mutants with abnormal leaves. Among a great number of leaf mutants we have generated by T-DNA and transposon tagging and ethylmethae sulfonate (EMS) mutagenesis, four independent mutant lines have been identified and studied genetically. Phenotypes of these mutant lines represent the defects of four novel nuclear genes designated LL1 (LOTUS LEAF 1), LL2 (LOTUS LEAF 2), URO (UPRIGHT ROSETTE), and EIL (ENVIRONT CONDITION INDUCED LESION). The phenotypic analysis indicates that these genes play important roles during leaf development. FOr the further genetic analysis of these genes and the map-based cloning of LL1 and LL2, we have mapped these genes to chromosome regions with an efficient and rapid mapping method. | SUN YUE WEI ZHANG FENG LING LI YING LI GUO TIAN LEI LIU HAI HUANG | 2000 | Cell Research2000，10，4： | 53 |
| 9 | Fast-track surgery could improve postoperative recovery in radical total gastrectomy patients显示文摘AIM: To assess the impact of fast-track surgery (FTS) on hospital stay, cost of hospitalization and complications after radical total gastrectomy. METHODS: A randomized, controlled clinical trial was conducted from November 2011 to August 2012 in the Department of Digestive Surgery, Xijing Hospital of Digestive Diseases, the Fourth Military Medical University. A total of 122 gastric cancer patients who met the selection criteria were randomized into FTS and conventional care groups on the first day of hospitalization. All patients received elective standard D2 total gastrectomy. Clinical outcomes, including duration of flatus and defecation, white blood cell count, postoperative pain, duration of postoperative stay, cost of hospitalization and complications were recorded and evaluated.Two specially trained doctors who were blinded to the treatment were in charge of evaluating postoperative outcomes, discharge and follow-up. RESULTS: A total of 119 patients finished the study, including 60 patients in the conventional care group and 59 patients in the FTS group. Two patients were excluded from the FTS group due to withdrawal of consent. One patient was excluded from the conventional care group because of a non-resectable tumor. Compared with the conventional group, FTS shortened the duration of flatus (79.03 ± 20.26 hvs 60.97 ± 24.40 h, P = 0.000) and duration of defecation (93.03 ± 27.95 h vs 68.00 ± 25.42 h, P = 0.000), accelerated the decrease in white blood cell count [P < 0.05 on postoperative day (POD) 3 and 4], alleviated pain in patients after surgery (P < 0.05 on POD 1, 2 and 3), reduced complications (P < 0.05), shortened the duration of postoperative stay (7.10 ± 2.13 dvs 5.68 ± 1.22 d,P = 0.000), reduced the cost of hospitalization (43783.25 ± 8102.36 RMBvs 39597.62 ± 7529.98 RMB,P = 0.005), and promoted recovery of patients. CONCLUSION: FTS could be safely applied in radical total gastrectomy to accelerate clinical recovery of gastric cancer patients. | Fan Feng Gang Ji Ji-Peng Li Xiao-Hua Li Hai Shi Zheng-Wei Zhao Guo-Sheng Wu Xiao-Nan Liu Qing-Chuan Zhao | 2013 | World Journal of Gastroenterology2013，19，23： | 44 |
| 10 | The Association of Overweight and Obesity with Blood Pressure among Chinese Children and Adolescents显示文摘Objective To examine the association between obesity and high blood pressure (BP) in Chinese children and adolescents. Methods Body mass index (BMI) and blood pressure measurements of 197 191 children aged 7-17 years were obtained from a Chinese national survey in 2010. Obesity and high BP were defined according to the reference values for Chinese children. Adjusted odds ratios (ORs) and 95% confidence intervals (CIs) of different BMI categories for high BP, as well as the population attributable risk percent (PAR%), were also calculated. Results The prevalence of high BP was 16.1% for boys and 12.9% for girls in 2010. Overweight and obese children had a significantly higher prevalence of high BP than non-overweight children in both boys and girls in each age group. ORs (95% CI) for high BP were 4.1 (3.9, 4.4) in obese boys and 4.0 (3.7, 4.3) in obese girls. The overall PAR% for high BP due to overweight and obesity was 14.4%. Conclusion Overweight and obese children have a significantly higher risk of high BP than non-overweight children. Eliminating overweight and obesity could reduce 14.4% of high BP cases. | DONG Bin MA Jun WANG Hai Jun WANG Zhi Qiang | 2013 | Biomedical and Environmental Sciences2013，26，6： | 42 |
| 11 | Anti-tumor activities of active ingredients in Compound Kushen Injection显示文摘Kushen (根值 Sophorae Flavescentis ) 在繁体中文药为肿瘤,发炎和另外的疾病的治疗有使用的长历史。复合 Kushen 注射(CKI ) 是从 Kushen 和 Baituling (根茎 Smilacis Glabrae ) 提取的自然混合物的混合物。CKI 的主要原则是 matrine (MT ) 和展出许多药理学活动的 oxymatrine (OMT ) ,包括反煽动性,反过敏,抗病毒, anti-fibrotic 和心血管的保护的效果。最近的证据证明这些混合物也产生反癌症行动,例如禁止癌症房间增长,导致房间周期拘捕,加速 apoptosis ,制止 angiogenesis ,导致房间区别,禁止癌症转移和侵略,逆行的 multidrug 抵抗,并且阻止或减少化疗--或导致放射疗法的毒性当与化学疗法的药结合了时。在这评论,我们在学习 MT, OMT 和 CKI 和他们的潜在的分子的目标的反癌症活动总结最近的进步,它为进一步的学习提供线索和参考书。 | Wei WANG Rong-li YOU Wen-jie QIN Li-na HAI Ming-jing FANG Guo-hua HUANG Rui-xia KANG Ming-hua LI Yu-feng QIAO Jian-wei LI An-ping LI | 2015 | Acta Pharmacologica Sinica2015，36，6： | 41 |
| 12 | A community-derived classification for extant lycophytes and ferns显示文摘发展史长通知了蕨类植物分类。当我们推断进化的树的能力改善了,针对认出生来的组的分类变得逐渐地预兆、稳定。这里,我们为 lycophytes 和蕨纲植物提供一个现代、全面分类,在下面,利用一条基于社区的途径类水平。我们 monophyly 用作主要标准让 taxa,而且目的识别保存两个广泛地被接受的存在 taxa 和界限并且与我们蕨类植物发展史的理解一致。总共,这个分类对待一在 337 个类, 51 个家庭, 14 目,和二个班估计了 11 916 种类。这个分类没在 lycophyte 和蕨纲植物上作为最后的词被打算分类,而是当前的假设的概括陈述,源于最好的可得到的数据并且在问题由熟悉植物的那些大多数塑造了。我们希望它将在蕨类植物上为最近的文学的那些想要的参考用作一个资源发展史和分类,为指导未来调查的一个框架,和推进讲话的刺激。 | Eric Schuettpelz Harald Schneider Alan R. Smith Peter Hovenkamp Jefferson Prado Germinal Rouhan Alexandre Salino Michael Sundue Thafs Elias Almeida Barbara Parris Emily B. Sessa Ashley R. Field Andre Luis de Gasper Carl J. Rothfels Michael D. Windham Marcus Lehnert Benjamin Dauphin Atsushi Ebihara Samuli Lehtonen Pedro Bond Schwartsburd Jordan Metzgar Li-Bing Zhang Li-Yaung Kuo Patrick J. Brownsey Masahiro Kato Marcelo Daniel Arana Francine C. Assis Michael S. Barker David S. Barrington Ho-Ming Chang Yi-Han Chang Yi-Shan Chao Cheng-Wei Chen De-Kui Chen Wen-Liang Chiou Vinicius Antonio de Oliveira Dittrich Yi-Fan Duan Jean-Yves Dubuisson Donald R. Farrar Susan Fawcett Jose Maria Gabriel y Galan Luiz Armando de Araujo Goes-Neto Jason R. Grant Amanda L. Grusz Christopher Haufler Warren Hauk Hai He Sabine Hennequin Regina Yoshie Hirai Layne Huiet Michael Kessler Petra Korall Paulo H. Labiak Anders Larsson Blanca Leen Chun-Xiang Li Fay-Wei Li Melanie Link-Perez Hong-Mei Liu Ngan Thi Lu Esteban I. Meza-Torres Xin-Yuan Miao Robbin Moran Claudine Massi Mynssens Nathalie Nagalingum Benjamin Ollgaard Alison M. Paul Jovani B. de S. Pereira Leon R. Perrie Monica Ponce Tom A. Ranker Christian Schulz Wataru Shinohara Alexander Shmakov Erin M. Sigel Filipe Soares de Souza Lana da Silva Sylvestre Weston Testo Luz Amparo Triana-Moreno Chie Tsutsumi Hanna Tuomisto IvAn A. Valdespino Alejandra Vasco Raquel Stauffer Viveros Alan Weakley Ran Wei Stina Weststrand Paul G. Wolf George Yatskievych Xiao-Gang Xu Yue-Hong Yan Liang Zhang Xian-Chun Zhang Xin-Mao Zhou | 2016 | Journal of Systematics and Evolution2016，54，6： | 44 |
| 13 | Why Lake Taihu continues to be plagued with cyanobacterial blooms through 10 years(2007–2017) efforts显示文摘With the expansion of urban, industry, and agriculture after World War II, eutrophication firstly emerged as a major water quality threat in small water bodies [1]. As the increasing magnitudes and scales of nutrient pollution and habitat alteration, many of the world's large lakes exhibit symptoms of eutrophication, e.g., toxic cyanobacterial blooms, deoxygenation, and habitat loss. | Boqiang Qin Hans W.Paerl Justin D.Brookes Jianguo Liu Erik Jeppesen Guangwei Zhu Yunlin Zhang Hai Xu Kun Shi Jianming Deng | 2019 | Science Bulletin2019，64，6： | 50 |
| 14 | Plant fertility defects induced by the enhanced expression of microRNA167显示文摘植物荷尔蒙植物生长素在调整植物生长和开发起一个关键作用。最近的进展在植物生长素反应小径的理解被做了,首先由在 Arabidopsis 的植物生长素反应异种的描述。另外, microRNAs (miRNAs ) 被显示了是为正常植物开发和生理学重要的基因的批评管理者。然而,很少对在 miRNAs 并且在正常开发期间的神经质的发信号之间的可能的相互作用被知道。这里,我们显示出那 Arabidopsis microRNA, miR167,有一个互补序列到 AUXIN 反应 FACTOR6 (ARF6 ) 和 ARF8 mRNAs 的部分,能为 ARF8,然而并非为 ARF6 引起抄本降级。我们报导 35S::MIR167b 的 phenotypic 描述转基因的线,和表演转基因的线有的那严重 35S::MIR167b arf6 arf8 的类似于那些的显型加倍异种。转基因的显型建议 miR167 可以镇压在翻译的水平的 ARF6。我们证明转基因的植物在花的机关的所有四锭盘是有缺点的。在转基因的花,细丝是反常地短的,花药不能适当地释放花粉,并且花粉谷物没发芽。我们的结果提供在表明网络支持的基因表示和植物生长素的调停 miRNA 的规章的小径之间的一个重要连接种繁殖开发。 | Peng Ru Lin Xu Hong Ma Hai Huang | 2006 | Cell Research2006，16，5： | 35 |
| 15 | A study on relationship of nitric oxide,oxidation,peroxidation,lipoperoxidation with chronic cholecystitis显示文摘AIM To study relationship of injury induced bynitric oxide,oxidation,peroxidation,lipoperoxidation with chronic cholecystitis.METHODS The values of plasma nitric oxide(P-NO),plasma vitamin C(P-VC),plasma vitamin E(P-VE),plasma β-carotene(P-β-CAR),plasmalipoperoxides(P-LPO),erythrocyte superoxidedismutase(E-SOD),erythrocyte catalase(E-CAT),erythrocyte glutathione peroxidase(E-GSH-Px)activities and erythrocyte lipoperoxides(E-LPO)level in 77 patients with chronic cholecystitisand 80 healthy control subjects were determined,differences of the above average values betweenthe patient group and the control group anddifferences of the average values betweenpreoperative and postoperative patients wereanalyzed and compared,linear regression andcorrelation of the disease course with the abovedetermination values as well as the stepwiseregression and correlation of the course with thevalues were analyzed.RESULTS Compared with the control group,theaverage values of P-NO,P-LPO,E-LPO weresignificantly increased(P<0.01),and of P-VC, P-VE,P-β-CAR,E-SOD,E-CAT and E-GSH-Pxdecreased(P<0.01)in the patient group.Theanalysis of the linear regression and correlationshowed that with prolonging of the course,thevalues of P-NO,P-LPO and E-LPO in the patientswere gradually ascended and the values of P-VC,P-VE,P-β-CAR,E-SOD,E-CAT and E-GSH-Pxdescended(P<0.01).The analysis of thestepwise regression and correlation indicated thatthe correlation of the course with P-NO,P-VE andP-β-CAR values was the closest.Compared withthe preoperative patients,the average values of P-NO,P-LPO and E-LPO were significantlydecreased(P<0.01)and the average values of P-VC,E-SOD,E-CAT and E-GSH-Px in postoperativepatients increased(P<0.01)in postoperativepatients.But there was no significant difference inthe average values of P-VE,P-β-CAR preoperativeand postoperative patients.CONCLUSION Chronic cholecystitis could inducethe increase of nitric oxide,oxidation,peroxidation and lipoperoxidation. | Jun Fu Zhou Dong Cai You Gen Zhu Jin Lu Yang Cheng Hong Peng Yang Hai Yu | 2000 | World Journal of Gastroenterology2000，6，4： | 36 |
| 16 | Buyang Huanwu decoction increases angiopoietin-1 expression and promotes angiogenesis and functional outcome after focal cerebral ischemia显示文摘 | Jian SHEN Yu ZHU Hai YU Zuo-xu FAN Feng XIAO Pan WU Qi-hui ZHANG Xiao-xing XIONG Jian-wei PAN Ren-ya ZHAN | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，3： | 36 |
| 17 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 18 | 经桡动脉穿刺的冠状动脉介入治疗显示文摘目的 探讨经桡动脉穿刺行冠状动脉介入治疗的可行性和安全性。方法 116例冠心病患者 (男 96例 ,女 2 0例 ,平均年龄 5 7 44± 9 40岁 )经桡动脉穿刺行冠状动脉介入治疗。结果 5例桡动脉穿刺失败改行股动脉穿刺 ,2例桡动脉穿刺虽成功但试行球囊扩张失败 ,其余患者均取得穿刺和介入治疗成功。共扩张病变血管 135支 (前降支 6 9支 ,回旋支 2 6支 ,右冠状动脉 39支 ,静脉桥血管1支 ) ,治疗病变 148处 (A型 15处 ,B型 10 6处 ,C型 2 7处 )。 98例患者的 10 5支血管植入支架共 110个 ,1例行旋磨术 ,1例行冠状动脉内超声检查。 2 5例患者术中须更换导引导管。术后穿刺局部大量出血需输血者 1例 ,局部较大血肿 2例 ,肘动脉分支穿孔 1例。结论 经桡动脉穿刺行冠状动脉介入治疗在临床上可行 ,但穿刺较困难 ,导引导管支撑力较差 ,应警惕局部血管损伤的可能。 | 洪涛 Koh Tian Hai Charles Chan Lim Yean-Leng | 2002 | 中国介入心脏病学杂志2002，10，3： | 39 |
| 19 | Strategies of minimally invasive treatment for intrahepatic and extrahepatic bile duct stones显示文摘 | Zongming Zhang Zhuo Liu Limin Liu Mengmeng Song Chong Zhang Hongwei Yu Baijiang Wan Mingwen Zhu Zixu Liu Hai Deng Haiming Yuan Haiyan Yang Wenping Wei Yue Zhao | 2017 | Frontiers of Medicine2017，11，4： | 37 |
| 20 | Does Helicobacter pylori infection play a role in iron deficiency anemia? A meta-analysis显示文摘AIM:To perform a meta-analysis of observational studies and randomized controlled trials(RCTs)on the association between Helicobacter pylori(H.pylori)and iron deficiency anemia(IDA).METHODS:A defined search strategy was used to search Medline,Embase,the Cochrane Library,Clinical Trials,Cochrane Central Register of Controlled Trials,Premedline and Healthstar.Odds ratio(OR)was used to evaluate observational epidemiology studies,and weighted mean difference(WMD)was used to demonstrate the difference between control and intervention groups.RESULTS:Fifteen observational studies and 5 RCTs were identified and used for calculation.The pooled OR for observational studies was 2.22(95%CI:1.52-3.24,P<0.0001).The WMD for hemoglobin(HB) was 4.06 g/L(95%CI:-2.57-10.69,P=0.01),and the WMD for serum ferritin(SF)was 9.47μg/L(95%CI:-0.50-19.43,P<0.0001).Results were heterogeneous for all comparisons.CONCLUSION:This meta-analysis on observational studies suggests an association between H.pylori and IDA.In RCTs,eradication of H.pylori can improve HB and SF levels but not significantly. | Qu, Xin-Hua Huang, Xiao-Lu Xiong, Ping Zhu, Cui-Ying Huang, You-Liang Lu, Lun-Gen Sun, Xu Rong, Lan Zhong, Liang Sun, Da-Yu Lin, Hai Cai, Ming-Ci Chen, Zhi-Wei Hu, Bing Wu, Lian-Ming Jiang, Yi-Bin Yan, Wei-Li | 2010 | World Journal of Gastroenterology2010，16，7： | 34 |
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