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| 1 | Different profiles of cytokine expression during mild and severe acute pancreatitis显示文摘AIM:To study secretion patterns of proand anti-in-flammatory cytokines, and activation of various cellular subsets of leukocytes in peripheral blood.METHODS: We have conducted a prospective obser-vational study. One hundred and eight patients with a diagnosis of acute pancreatitis and onset of the disease within last 72 h were included in this study. The mRNA expression of 25 different types of cytokines in white blood cells was determined by quantitative real time polymerase chain reaction. Levels of 8 different cytokines in blood serum were measured by enzymelinked immunosorbent assay. Clinical data and cytokine expression results were subjected to statistical analysis.RESULTS: Severe and necrotizing acute pancreatitis (AP) is characterized by the significant depletion of circulating lymphocytes. Severe acute pancreatitis is as-sociated with a typical systemic inflammatory response syndrome and over-expression of pro-inflammatory cy-tokines [interleukin (IL)-6, IL-8, macrophage migration inhibitory factor (MIF)]. Serum IL-6 and MIF concentra-tions are the best discriminators of severe and necrotiz-ing AP as well as possible fatal outcome during the early course of the disease. CONCLUSION: Deregulation of cellular immune sys-tem is a key event leading to severe and necrotizing AP. IL-6 and MIF could be used as early predictors of complications. | Zilvinas Dambrauskas Nathalia Giese Antanas Gulbinas Thomas Giese Pascal O Berberat Juozas Pundzius Giedrius Barauskas Helmut Friess | 2010 | World Journal of Gastroenterology2010，16，15： | 49 |
| 2 | Early recognition of abdominal compartment syndrome in patients with acute pancreatitis显示文摘AIM:To assess the value of widely used clinical scores in the early identification of acute pancreatitis(AP) patients who are likely to suffer from intra-abdominal hypertension(IAH)and abdominal compartment syndrome(ACS). METHODS:Patients(n=44)with AP recruited in this study were divided into two groups(ACS and non-ACS) according to intra-abdominal pressure(IAP)determined by indirect measurement using the transvesical route via Foley bladder catheter.On admission and at regular intervals,the severity of the AP and presence of organ dysfunction were assessed utilizing different multifactorial prognostic systems:Glasgow-Imrie score,Acute Physiology and Chronic Health Evaluation Ⅱ(APACHE-Ⅱ)score,and Multiorgan Dysfunction Score(MODS).The diagnostic performance of scores predicting ACS development,cut-off values and specificity and sensitivity were established using receiver operating characteristic(ROC)curve analysis. RESULTS:The incidence of ACS in our study population was 19.35%.IAP at admission in the ACS group was 22.0(18.5-25.0)mmHg and 9.25(3.0-12.4)mmHg in the non-ACS group(P<0.01).Univariate statistical analysis revealed that patients in the ACS group had significantly higher multifactorial clinical scores(APACHE Ⅱ,Glasgow-Imrie and MODS)on admission and highermaximal scores during hospitalization(P<0.01).ROC curve analysis revealed that APACHEⅡ,Glasgow-Imrie, and MODS are valuable tools for early prediction of ACS with high sensitivity and specificity,and that cut- off values are similar to those used for stratification of patients with severe acute pancreatitis(SAP). CONCLUSION:IAH and ACS are rare findings in patients with mild AP.Based on the results of our study we recommend measuring the IAP in cases when patients present with SAP(APACHEⅡ>7;MODS>2 or Glasgow-Imrie score>3). | Zilvinas Dambrauskas Audrius Parseliunas Antanas Gulbinas Juozas Pundzius Giedrius Barauskas | 2009 | World Journal of Gastroenterology2009，15，6： | 32 |
| 3 | Narrow line between benefit and harm: Additivity of hyperthermia to cisplatin cytotoxicity in different gastrointestinal cancer cells显示文摘AIM To investigate the response to hyperthermia and chemotherapy, analyzing apoptosis, cytotoxicity, and cisplatin concentration in different digestive system cancer cells.METHODS AGS(gastric cancer cell line), Caco-2(colon cancer cell line) and T3M4(pancreatic cancer cell line) were treated by cisplatin and different temperature setting(37 ℃ to 45 ℃) either in isolation, or in combination. Treatment lasted for one hour. 48 h after the treatment viability was evaluated by MTT, cell apoptosis by Annexin V-PE and 7 ADD flow cytometry. Intracellular cisplatin concentration was measured immediately after the treatment, using mass spectrometry. Isobologram analysis was performed to evaluate the mathematical combined effect of temperature and cisplatin.RESULTS AGS cells were the most sensitive to isolated application of hyperthermia. Hyperthermia, in addition to cisplatin treatment, did not provoke a synergistic effect at intervals from 37 ℃ to 41 ℃ in neither cancer cell line. However, a temperature of 43 ℃ enhanced cisplatin cytotoxicity for Caco-2 cells. Moreover, isobologram analysis revealed mathematical antagonistic effects of cisplatin and temperature combined treatment in AGS cells; variations between synergistic, additive, and antagonistic effects in Caco-2 cells; and additive and antagonistic effects in T3 M4 cells. Combined treatment enhanced initiation of cell apoptosis in AGS, Caco-2, and T3 M4 cells by 61%, 20%, and 19% respectively. The increase of intracellular cisplatin concentration was observed at 43 ℃ by 30%, 20%, and 18% in AGS, Caco-2, and T3 M4 cells, respectively.CONCLUSION In addition to cisplatin, hyperthermia up to 43 ℃ does not affect the viability of cancer cells in a synergistic manner. | Vaidotas Cesna Arturas Sukovas Aldona Jasukaitiene Rima Naginiene Giedrius Barauskas Zilvinas Dambrauskas Saulius Paskauskas Antanas Gulbinas | 2018 | World Journal of Gastroenterology2018，24，10： | 6 |
| 4 | HuR mediated post-transcriptional regulation as a new potential adjuvant therapeutic target in chemotherapy for pancreatic cancer显示文摘AIM: To investigate the expression of Hu R in pancreatic ductal adenocarcinoma(PDA) and to assess the effects of Hu R silencing on the expression of cyclooxygenase-2(COX-2) and heme oxygenase-1(HO-1) and the in vitro response to gemcitabine(GEM) treatment in pancreatic cell lines.METHODS: We compared the expression of Hu R,COX-2,and HO-1 in PDA and normal pancreatic tissueusing quantitative reverse transcription polymerase chain reaction(q RT-PCR) and western blot. In addition,the Hu R,COX-2 and HO-1 were analyzed in four types of cancer cell lines(Mia Paca2,Su.86.86,Capan-1,and Capan-2) with and without GEM treatment. Immunocytofluorescence analysis was used to investigate Hu R localization in cells. Cell viability and response to GEM after Hu R silencing were determined with the 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide test and the crystal violet clonogenic assay,respectively. To measure apoptosis,activation of caspases 3/7 was evaluated using immunofluorescence. RESULTS: In PDA tissue obtained from patients not treated with GEM,Hu R m RNA expression was 3.2 times lower(P < 0.05) and COX-2 and HO-1 m RNAexpression was 2.3-fold and 7.2-fold higher(P < 0.05),respectively,than normal pancreatic tissue(from organ donor). q RT-PCR analysis showed that Hu R,COX-2,and HO-1 m RNA were overexpressed in all cancer cell lines treated with the half maximal inhibitory concentration(IC50) dose of GEM compared with control cells(P < 0.05). Western blot analysis revealed that COX-2 and HO-1 levels were significantly decreased in cancer cells after Hu R silencing. Furthermore,HuR silencing in creased the response to GEM treatmentand decreased cell viability by 11.6%-53.7% compared to control cell lines. Caspases 3 and 7 were activated after Hu R silencing and GEM treatment in all pancreatic cancer cell lines. In comparison,treatment with GEM alone did not activate caspases 3 and 7 in the same cell lines. CONCLUSION: Hu R mediated post-transcriptional upregulation of COX-2 and HO-1 expression after GEM treatment in pancreatic cancer cells. Hu R silencing significantly increased the effectiveness of GEM treatment in vitro. | Aldona Jakstaite Aurelija Maziukiene Giedre Silkuniene Kristina Kmieliute Antanas Gulbinas Zilvinas Dambrauskas | 2015 | World Journal of Gastroenterology2015，21，46： | 4 |
| 5 | Fatty acids of erythrocyte membrane in acute pancreatitis patients显示文摘AIM:To evaluate changes in the fatty acid composition of erythrocyte membrane phospholipids during severe and mild acute pancreatitis(AP)of alcoholic and nonalcoholic etiology.METHODS:All consecutive patients with a diagnosis of AP and onset of the disease within the last 72 h admitted to the Hospital of Lithuanian University of Health Sciences between June and December 2007 were included.According to the Acute Physiology and ChronicHealth Evaluation(APACHEⅡ)scale,the patients were subdivided into the mild(APACHEⅡscore<7,n=22)and severe(APACHEⅡscore≥7,n=17)AP groups.Healthy individuals(n=26)were enrolled as controls.Blood samples were collected from patients on admission to the hospital.Fatty acids(FAs)were extracted from erythrocyte phospholipids and expressed as percentages of the total FAs present in the chromatogram.The concentrations of superoxide dismutase and glutathione peroxidase were measured in erythrocytes.RESULTS:We found an increase in the percentages of saturated and monounsaturated FAs,a decrease in the percentages of total polyunsaturated FAs(PUFAs)and n-3 PUFAs in erythrocyte membrane phospholipids of AP patients compared with healthy controls.Palmitic(C16:0),palmitoleic(C16:1n7cis),arachidonic(C20:4n6),docosahexaenoic(DHA,C22:6n3),and docosapentaenoic(DPA,C22:5n3)acids were the major contributing factors.A decrease in the peroxidation and unsaturation indexes in AP patients as well as the severe and mild AP groups as compared with controls was observed.The concentrations of antioxidant enzymes in the mild AP group were lower than in the control group.In severe AP of nonalcoholic etiology,the percentages of arachidic(C20:0)and arachidonic(C20:4n6)acids were decreased as compared with the control group.The patients with mild AP of nonalcoholic etiology had the increased percentages of total saturated FAs and gama linoleic acid(C18:3n6)and the decreased percentages of elaidic(C18:1n9t),eicosapentaenoic acid(EPA,C20:5n3),DPA(C22:5n3),DHA(C22:6n3)as well as total and n-3 PUFAs in erythrocyte membrane phospholipids.CONCLUSION:The composition of FAs in erythrocyte membranes is altered during AP.These changes are likely to be associated with alcohol consumption,inflammatory processes,and oxidative stress. | Irma Kuliaviene Antanas Gulbinas Johannes Cremers Juozas Pundzius Limas Kupcinskas Zilvinas Dambrauskas Eugene Jansen | 2013 | World Journal of Gastroenterology2013，19，34： | 3 |
| 6 | Fast Track—Different Implications in Pancreatic Surgery显示文摘 | P. O. Berberat H. Ingold A. Gulbinas J. Kleeff M. W. Müller C. Gutt M. Weigand H. Friess M. W. Büchler | 2007 | Journal of Gastrointestinal Surgery2007，，： | 2 |
| 7 | Meta-analysis of prophylactic parenteral antibiotic use in acute necrotizing panereatitis显示文摘 | Dambrauskas Z Gulbinas A Pundzius J | 2007 | Medicina (Kaunas)2007，43，4： | 1 |
| 8 | Radiofrequency abla- tion of liver tumors (I): biological background 显示文摘 | Vanagas T Gulbinas A Pundzius J | 2010 | Medicina (Kaunas)2010，46，1： | 1 |
| 9 | Congenital hyperinsulinism 显示文摘 | Petraitiene I Barauskas G Gulbinas A | 2014 | Medicina (Kaunas)2014，50，3： | 1 |
| 10 | The impact of morphologic and physiologic peculiarities of the pancreas on pancreas-related complications following pancreatoduodenectomy显示文摘 | Gulbinas A Barauskas G Pundzius J | 2003 | Medicina (Kaunas)2003，39，2： | 1 |
| 11 | Meta-analysis of prophylactic parenteral antibiotic use in acute necrotizing pancreatitis显示文摘 | Dambrauskas Z Gulbinas A Pundzius J | 2007 | Medicina:Kaunas2007，43，4： | 1 |
| 12 | Value of routine clinical tests in predicting the development of infected pancreatic necrosis in severe acute pancreatitis 显示文摘 | Dambrauskas Z Gulbinas A Pundzius J | 2007 | Scand ''J Gastroenterol2007，42，1： | 1 |
| 13 | Fast track--different implications in pancreatic surgery显示文摘 | Berberat PO Ingold H Gulbinas A | 2007 | J Gastrointest Surg2007，11，7： | 1 |
| 14 | Inhibition of heme oxygenase-1 increases responsiveness of pancreatic cancer cells to anticancer treatment 显示文摘 | BERBERAT P O DAMBRAUSKAS Z GULBINAS A | 2005 | Clin Cancer Res2005，11，10： | 1 |
| 15 | Radiofrequency ablation of liver tumors (11) : clinical application and outcomes 显示文摘 | Vanagas T Gulbinas A Pundzius J | 2010 | Medici- ha( Kaunas Lithuania)2010，46，2： | 1 |
| 16 | Value of the different prognostic systems and biological markers for predicting severity and progression of acute pancreatitis显示文摘 | Dambrauskas Z Gulbinas A Pundzius J | | 0，，7： | 1 |
| 17 | Value of routine clinical tests in predicting the development of infected pancreatic necrosis in severe acute pancreatitis 显示文摘 | Dambrauskas Z Gulbinas A Pundzius J | 2007 | Scand J Gastroenterol2007，42，1： | 1 |
| 18 | The impact of morphologic and physiologic peculiarities of the pancreas on pancreasrelated complications following pancreatoduodenectomy 显示文摘 | Gulbinas A Barauskas G Pundzius J | 2003 | Medicina(Kaunas)2003，39，2： | 1 |
| 19 | Inhibition of heine oxygenase-1 increases responsiveness of pancreatic cancer cells to antieancer treatment显示文摘 | Berberat P O Dambrauskas Z Gulbinas A | 2005 | Clin Cancer Res2005，11，10： | 1 |
| 20 | Value of routine clinical testsin predicting the development of infected pancreatic necrosis in severe acute pancreatitis 显示文摘 | Dambrauskas Z Gulbinas A Pundzius J | 2007 | Scand J Gastroenterol2007，42，10： | 1 |
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